295 research outputs found
Kinetostatic Analysis and Solution Classification of a Planar Tensegrity Mechanism
Tensegrity mechanisms have several interesting properties that make them
suitable for a number of applications. Their analysis is generally challenging
because the static equilibrium conditions often result in complex equations. A
class of planar one-degree-of-freedom (dof) tensegrity mechanisms with three
linear springs is analyzed in detail in this paper. The kinetostatic equations
are derived and solved under several loading and geometric conditions. It is
shown that these mechanisms exhibit up to six equilibrium configurations, of
which one or two are stable. Discriminant varieties and cylindrical algebraic
decomposition combined with Groebner base elimination are used to classify
solutions as function of the input parameters.Comment: 7th IFToMM International Workshop on Computational Kinematics, May
2017, Poitiers, France. 201
Co-evolution of RDF Datasets
Linking Data initiatives have fostered the publication of large number of RDF
datasets in the Linked Open Data (LOD) cloud, as well as the development of
query processing infrastructures to access these data in a federated fashion.
However, different experimental studies have shown that availability of LOD
datasets cannot be always ensured, being RDF data replication required for
envisioning reliable federated query frameworks. Albeit enhancing data
availability, RDF data replication requires synchronization and conflict
resolution when replicas and source datasets are allowed to change data over
time, i.e., co-evolution management needs to be provided to ensure consistency.
In this paper, we tackle the problem of RDF data co-evolution and devise an
approach for conflict resolution during co-evolution of RDF datasets. Our
proposed approach is property-oriented and allows for exploiting semantics
about RDF properties during co-evolution management. The quality of our
approach is empirically evaluated in different scenarios on the DBpedia-live
dataset. Experimental results suggest that proposed proposed techniques have a
positive impact on the quality of data in source datasets and replicas.Comment: 18 pages, 4 figures, Accepted in ICWE, 201
Discordant bioinformatic predictions of antimicrobial resistance from whole-genome sequencing data of bacterial isolates: an inter-laboratory study.
Antimicrobial resistance (AMR) poses a threat to public health. Clinical microbiology laboratories typically rely on culturing bacteria for antimicrobial-susceptibility testing (AST). As the implementation costs and technical barriers fall, whole-genome sequencing (WGS) has emerged as a 'one-stop' test for epidemiological and predictive AST results. Few published comparisons exist for the myriad analytical pipelines used for predicting AMR. To address this, we performed an inter-laboratory study providing sets of participating researchers with identical short-read WGS data from clinical isolates, allowing us to assess the reproducibility of the bioinformatic prediction of AMR between participants, and identify problem cases and factors that lead to discordant results. We produced ten WGS datasets of varying quality from cultured carbapenem-resistant organisms obtained from clinical samples sequenced on either an Illumina NextSeq or HiSeq instrument. Nine participating teams ('participants') were provided these sequence data without any other contextual information. Each participant used their choice of pipeline to determine the species, the presence of resistance-associated genes, and to predict susceptibility or resistance to amikacin, gentamicin, ciprofloxacin and cefotaxime. We found participants predicted different numbers of AMR-associated genes and different gene variants from the same clinical samples. The quality of the sequence data, choice of bioinformatic pipeline and interpretation of the results all contributed to discordance between participants. Although much of the inaccurate gene variant annotation did not affect genotypic resistance predictions, we observed low specificity when compared to phenotypic AST results, but this improved in samples with higher read depths. Had the results been used to predict AST and guide treatment, a different antibiotic would have been recommended for each isolate by at least one participant. These challenges, at the final analytical stage of using WGS to predict AMR, suggest the need for refinements when using this technology in clinical settings. Comprehensive public resistance sequence databases, full recommendations on sequence data quality and standardization in the comparisons between genotype and resistance phenotypes will all play a fundamental role in the successful implementation of AST prediction using WGS in clinical microbiology laboratories
Growth dynamics and the evolution of cooperation in microbial populations
Microbes providing public goods are widespread in nature despite running the
risk of being exploited by free-riders. However, the precise ecological factors
supporting cooperation are still puzzling. Following recent experiments, we
consider the role of population growth and the repetitive fragmentation of
populations into new colonies mimicking simple microbial life-cycles.
Individual-based modeling reveals that demographic fluctuations, which lead to
a large variance in the composition of colonies, promote cooperation. Biased by
population dynamics these fluctuations result in two qualitatively distinct
regimes of robust cooperation under repetitive fragmentation into groups.
First, if the level of cooperation exceeds a threshold, cooperators will take
over the whole population. Second, cooperators can also emerge from a single
mutant leading to a robust coexistence between cooperators and free-riders. We
find frequency and size of population bottlenecks, and growth dynamics to be
the major ecological factors determining the regimes and thereby the
evolutionary pathway towards cooperation.Comment: 26 pages, 6 figure
Gridshell as Formwork: Proof of Concept for a New Technique for Constructing Thin Concrete Shells Supported by Gridshell as Formwork
This paper documents an empirical experiment conducted in August 2014 as proof of concept for a new method of constructing concrete shells. An idea initially presented by the first author in 2012, it uses redeployable gridshells onto which fabric is midstressed and concrete applied. Primarily, this system addresses key issues that led to their decline in use: construction methods/formwork systems were not reusable, nor were they easily customizable to create different shapes. Employing 27 man-hours over seven days, two concrete shells were achieved using the same reusable and reconfigurable formwork. Lightweight (0.6 kg) PVC gridshell formwork supported 106.92 kg of concrete to create a concrete shell that covered 1.11 m2 (floor area). The construction verifies a low-cost (ÂŁ6.06/m2) efficiency and material utilization in the construction of very strong wide-spanning thin concrete structures. Detailed analysis of formwork behavior during construction and detailed measurements of resultant shell results prove this new method of deployable gridshells as a reusable and reconfigurable formwork to construct very strong concrete shells very quickly. Whilst the emphasis of the research focused on the construction process, the vaults were tested and sustained a failure load of 4.2 kN (4.32 times their deadweight), applied as a point load at the crown
The fickle Mutation of a Cytoplasmic Tyrosine Kinase Effects Sensitization but not Dishabituation in Drosophila Melanogaster
fickle is a P-element mutation identified from a screen for defects in courtship behavior and disrupts the fly homolog of Bruton's tyrosine kinase (Btk) gene (Baba et al., 1999). Here, we show that habituation of the olfactory jump reflex also is defective in fickle. Unlike, the prototypical memory mutants, rutabaga and dunce, which habituate more slowly than normal, fickle flies habituate faster than normal. fickle's faster-than-normal response decrement did not appear to be due to sensorimotor fatigue, and dishabituation of the jump response was normal. Based on a long-standing âtwo opponent processâ theory of habituation, these data suggested that behavioral sensitization might be defective in fickle. To test this hypothesis, we designed a olfactory sensitization procedure, using the same stimuli to habituate (odor) and dishabituate (vortexing) flies. Mutant flies failed to show any sensitization with this procedure. Our study reveals a âgenetic dissectionâ of sensitization and dishabituation and, for the first time, provides a biological confirmation of the two opponent process theory of habituation
Role of the electro-thermo-mechanical multiple coupling on the operation of RF microswitch
A phenomenological approach is proposed to identify some effects occurring within the structure of the microswitch conceived for radio frequency application. This microsystem is operated via a nonlinear electromechanical action imposed by the applied voltage. Unfortunately, it can be affected by residual stress, due to the microfabrication process, therefore axial and flexural behaviors are strongly coupled. This coupling increases the actuation voltage required to achieve the so-called ââpull-in'' condition. Moreover, temperature may strongly affect strain and stress distributions, respectively. Environmental temperature, internal dissipation of material, thermo-elastic and Joule effects play different roles on the microswitch flexural isplacement. Sometimes buckling phenomenon evenly occurs. Literature show that all those issues make difficult an effective computation of ââpull-in'' and ââpull-out'' voltages for evenly distinguishing the origin of some failures detected in operation. Analysis, numerical methods and experiments are applied to an industrial test case to investigate step by step the RF-microswitch operation. Multiple electro-hermomechanical coupling is first modeled to have a preliminary and comprehensive description of the microswitch behavior and of its reliability. ââPull-in'' and ââpull-out'' tests are then performed to validate the proposed models and to find suitable criteria to design the RF-MEM
- âŠ