Does the anaesthetic influence behavioural transmission of the monogenean Gyrodactylus gasterostei Glaser, 1974 off the host?

The aim of this study was to investigate the use of the anaesthetic 2-phenoxyethanol on the transmission factors of gyrodactylid and to ascertain how this may affect in the colonisation of new hosts using the Gyrodactylus gasterostei Glaser, 1974 - Gasterosteus aculeatus L. model which is a simple and successful system to examine aspects of transmission of parasites from live and dead fish. Laboratory experiments include determining the maturity (presence of male copulate organ) and reproductive (presence of daughter) status of transmitting worms, in order to consider the factors that influence parasite option to migrate to a new individual of the same host species. This study demonstrates that parasites with a Male Copulate Organ (MCO) present are more likely to abandon the host and attempt a host transfer. The use of the anaesthetic 2-phenoxyethanol does not affect transmission of gyrodactylids which leave the host to colonise a new host. Finally, the use of other anaesthetic although its relative importance with respect to transmission remains uncertain.Department of the Environment, Food and Rural Affairs (Defra); Overseas Research Students Awards Scheme (ORSAS) UK; Consejo Nacional Ciencia y Tecnologia, Mexico CONACyT [171032]info:eu-repo/semantics/publishedVersio

Indirect ELISA for Detection of Fascioliasis IgG Antibodies in Human Sera

Currently, coprological examination based on egg detection in stool samples is used as the most ideal standard for the diagnosis of human fascioliasis. However, this method has been proven not to be adequate when being employed in the acute phase of the disease, and presents a poor sensitivity during the chronic phase. Serodiagnosis has become an excellent alternative to coprological examination in efforts to combat the effects of fascioliasis on human and animal health. Human fascioliasis is usually recognized as an infection of the bile ducts and liver caused by Fasciola hepatica, known to affect over 2 million humans. In this research, Indirect Enzyme-linked Immunosorbent Assay (ELISA) was performed to discern between positive and negative IgG antibody titers in sera in collaboration with a lab in Peru. A batch of approximately 325 samples of human sera of endemic Fasciola hepatica from regions in Peru was gathered and sent to the laboratory in order to be examined with the method explained in the following paragraph. In the search for a test for the diagnosis of fascioliasis on humans, indirect ELISA started to be employed in order to determine positive and negative values. In the indirect ELISA test, the sample antibody is sandwiched between the antigen coated on the plate and an enzyme-labeled, anti-species globulin conjugate. The addition of an enzyme substrate-chromogen reagent causes color to develop. This color is directly proportional to the amount of bound sample antibody. The more antibody present in the sample, the stronger the color development in the test wells. Positive samples presented a very strong optical density value, while negative samples were clear or low optical density value, measured with the aid of a spectrophotometer

Specifying Exposure Classification Parameters for Sensitivity Analysis: Family Breast Cancer History

One of the challenges to implementing sensitivity analysis for exposure misclassification is the process of specifying the classification proportions (eg, sensitivity and specificity). The specification of these assignments is guided by three sources of information: estimates from validation studies, expert judgment, and numerical constraints given the data. The purpose of this teaching paper is to describe the process of using validation data and expert judgment to adjust a breast cancer odds ratio for misclassification of family breast cancer history. The parameterization of various point estimates and prior distributions for sensitivity and specificity were guided by external validation data and expert judgment. We used both nonprobabilistic and probabilistic sensitivity analyses to investigate the dependence of the odds ratio estimate on the classification error. With our assumptions, a wider range of odds ratios adjusted for family breast cancer history misclassification resulted than portrayed in the conventional frequentist confidence interval.Children's Cancer Research Fund, Minneapolis, MN, US

Traveling wave solutions for wave equations with two exponential nonlinearities

We use a simple method that leads to the integrals involved in obtaining the traveling wave solutions of wave equations with one and two exponential nonlinearities. When the constant term in the integrand is zero, implicit solutions in terms of hypergeometric functions are obtained while when that term is nonzero all the basic traveling wave solutions of Liouville, Tzitzeica and their variants, as well as sine/sinh-Gordon equations with important applications in the phenomenology of nonlinear physics and dynamical systems are found through a detailed study of the corresponding elliptic equationsComment: 9 pages, 7 figures, 42 references, version matching the published articl

A binocular-type atmospheric interaction generating sequential exotic features

A cosmic-ray induced nuclear event is presented, which is of clear binocular-type and contains several exotic features through its passage in the atmosphere and the emulsion chamber