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Proximal chemical analysis and secondary metabolites in Washingtonia robusta fruit (Arecaceae): relevance for the feeding of wildlife and human
Background: Washingtonia robusta fruits are a staple for birds, small and medium-size mammals, and humans. However, quantitative data of their nutritional contribution has remained unknown. This is the first report on the phytochemical components found in fruit pulp and seeds. Questions: Does pulp compounds favor its consumption and contribute with nutrients for wildlife and humans? Do seed contents favor ecological interactions for germination and plantlet development? Species study: Washingtonia robusta H. Wendl. Study site and dates: Sierra El Aguaje, Sonora, Mexico, February - April 2016 Methods: Chemical and secondary metabolite analyses were carried out from extracts via phytochemical coloring tests and ultra-resolution liquid chromatography. Antioxidant activity was analyzed by DPPH. Results: Washingtonia robusta seeds contain 73 % sugars, 7.4 % protein, 8.4 % humidity, 4.3 % ashes, 8.7 % fat and 0.2 % calcium, while cyanogenic glycosides content was 0.8 mu g/g. Pulp has 71 % sugars, 10.8 % protein, 1.6 % humidity, 5.5 % ashes, 9.4 % fat and 1.5 % calcium, and cyanogenic glycosides reached 0.2 mu g/g. Alkaloids, tannins, saponins, phenols, and flavonoids were more abundant in seeds than in pulp. The DPPH assay expressed in Trolox equivalents indicated antioxidant capacity. Conclusions: Washingtonia robusta pulp is an important source of sugars and natural antioxidants for wildlife and human consumption. Occurrence of secondary metabolites prevents pathogens in seeds, also they could be beneficial for germination and initial plantlet development.Open access journalThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at [email protected]
Rigorous Analysis of Available Data from Cerro Prieto and Las Tres Virgenes Geothermal Fields with Calculations for Expanded Electricity Generation
Effect of parsley (Petroselinum crispum, Apiaceae) juice against cadmium neurotoxicity in albino mice (Mus Musculus)
Mother-fetus transference of lead and cadmium in rats, involvement of metallothionein
This study was designed to assess the effect
of Cadmium (Cd) and lead (Pb) exposure during
pregnancy in rats and their correlation with
metallothionein (MT). Rats were exposed to either 10
ppm Cd or 300 ppm Pb through drinking water during
pregnancy. Both metals were measured in placenta, fetus
brain and fetal and maternal blood. MT was quantified in
placenta and fetus brain and it was also observed in
placenta by immunohistochemical technique. Offspring
weight was found to be significantly lower for the Cd
exposure group than for the control group. A Cd increase
in the placenta of the exposed group was accompanied
by MT induction; these effects were related to a limited
accumulation of Cd in fetus brain. In contrast, dam Pb
exposure caused an accumulation of Pb in the fetus brain
and induced damage to placenta. The results account for
differences in the transference of these metals during
pregnancy that could be related to their toxicity
Morphometric analysis of brain lesions in rat fetuses prenatally exposed to low-level lead acetate: correlation with lipid peroxidation
The effect of prenatal lead acetate exposure
was studied microscopically together with the
concentration of lead and lipid fluorescent products
(LFP) in the brain of rat fetuses. Wistar rats were
intoxicated with a lead solution containing either 160 or
320 ppm of lead acetate solution during 21 days through
drinking water. The control group (ten rats) received
deionized water for the same period. The rats were killed
on gestation day 21 and fetuses were obtained; the
placenta, umbilical cord and parietal cortex (Cx),
striatum (St), thalamus (Th) and cerebellum (Ce) were
collected for measuring tissue lead concentration, LFP as
an index of lipid peroxidation and histopathologic
examination. Lead contents were increased in placenta,
umbilical cord, St, Th and Cx in both lead-exposed
groups. Lead exposure increased (LFP) in placenta and
umbilical cord, St, Th and Ce as compared to the control
group. Histopathological examination showed severe
vascular congestion in placenta, the Cx, St, Th and Ce
with hyperchromatic and shrunken cells. Interstitial
oedema was found in all regions studied of both lead
exposed groups. The morphometric evaluation of the
studied brain regions showed an absolute decrease in
total cell number and increased number of damaged cells and interstitial oedema. Our results show that
morphological changes in rat brain are correlated with
increased lipid peroxidation, and the lead levels of the
umbilical cord, however it is not clear whether oxidative
stress is the cause or the consequence of these
neurotoxic effects of lead
Differences in firing efficiency, chromatin, and transcription underlie the developmental plasticity of the Arabidopsis DNA replication origins
Eukaryotic genome replication depends on thousands of DNA replication origins (ORIs). A major challenge is to learn ORI biology in multicellular organisms in the context of growing organs to understand their developmental plasticity. We have identified a set of ORIs of Arabidopsis thaliana and their chromatin landscape at two stages of post-embryonic development. ORIs associate with multiple chromatin signatures including transcription start sites (TSS) but also proximal and distal regulatory regions and heterochromatin, where ORIs colocalize with retrotransposons. In addition, quantitative analysis of ORI activity led us to conclude that strong ORIs have high GC content and clusters of GGN trinucleotides. Development primarily influences ORI firing strength rather than ORI location. ORIs that preferentially fire at early developmental stages colocalize with GC-rich heterochromatin, but at later stages with transcribed genes, perhaps as a consequence of changes in chromatin features associated with developmental processes. Our study provides the set of ORIs active in an organism at the post-embryo stage that should allow us to study ORI biology in response to development, environment, and mutations with a quantitative approach. In a wider scope, the computational strategies developed here can be transferred to other eukaryotic systems.This research was supported by Secretaría de Estado de Investigación, Desarrollo e Innovación, grants BFU2012-34821, BFU2013-50098-EXP, and BFU2015-68396-R to
C.G., BIO2016-79043-P to U.B. and AGL2013-43244-R to J.M.C., as well as by institutional grants from Fundacion Ramon Areces and Banco de Santander to the CBMSO.Peer reviewe