1,610 research outputs found
Comparative studies of the nucleotide sequences of pea seed storage protein genes
Nucleotide sequence data from several pea (Piswn sativum L.) seed storage protein genes was obtained. Of two legumin genes sequenced, one was shown to be a pseudogene, apparently once coding for a polypeptide belonging to the 'major' legumin class, whilst the other was shown to be a functional gene coding for a polypeptide of the 'minor' legumin class. Sequence data was also obtained for two vicilin genes. Complete sequencing of one revealed it to be truncated by sequence of unknown origin at its 3' end, whilst partial sequence for the other suggested the presence of a stop codon in the coding region. These findings implied that both vicilin genes are no longer functional. Additionally, various comparisons of nucleic acid and amino acid sequence data were made between these genes and also with other legume seed storage protein genes. Results showed these genes conform with the major structural features of eukaryotic genes, and also revealed the presence of potential tissue -specific regulatory elements in the 5' flanking regions of the genes. Dendrograms for legume 11S and 7S classes of globulin seed storage protein genes clearly supported the model theory of each class having arisen by successive duplications from a common ancestral gene
Evaluating the Rapid Divergence of Male Genitalia in Sibling Drosophila Species
The rapid divergence of male genitalia in a variety of animal groups is a well documented phenomenon for which there exists no universal explanation. The three prevalent hypotheses for the divergence of genitalia, the lock and key, pleiotropy and sexual selection, have all been tested in individual model organisms, but never have individual experiments been performed in one species pair to allow for direct comparison. Drosophila simulans and D. mauritiana have long been thought to be an example of the sensory lock and key model, but no concrete data has ever been presented to verify the validity of the model. This work looks to employ three different techniques to investigate each of the hypotheses, disproving the long held lock and key model and instead supporting the more generally accepted sexual selection hypothesis. These techniques could help to apply a more general explanation for the rapid divergence of male genitalia
Polaron relaxation in ferroelectric thin films
We report a dielectric relaxation in ferroelectric thin films of the ABO3
family. We have compared films of different compositions with several growth
modes: sputtering (with and without magnetron) and sol-gel. The relaxation was
observed at cryogenic temperature (T<100K) for frequencies from 100Hz up to
10MHz. This relaxation activation energy is always lower than 200meV. It is
very similar to the polaron relaxation that we reported in the parent bulk
perovskites. Being independent of the materials size, morphology and texture,
this relaxation can be a useful probe of defects in actual integrated
capacitors with no need for specific shapin
Tidal currents, winds and the morphology of phytoplankton spatial structures
Chlorophyll a, nutrients and salinity distributions were studied at two spatial scales (10 cm and 0.25 to 2.5 km) in the St. Lawrence Estuary (Quebec, Canada), in order to investigate the role of tidal currents and winds in the formation and maintenance of spatial structures. Data were collected according to a synoptic sampling pattern using three sampling platforms simultaneously, and they were analyzed using analysis of variance. The sampling pattern was repeated on four occasions during July 1980.Analyses of variance indicated significant spatial heterogeneities of about the same magnitude at the two scales studied for chlorophyll and nutrients, whereas salinity showed only large–scale variability. At the kilometer scale, the frequency distribution spectra of patch length for chlorophyll showed the existence of patches of various dimensions between 0.2 and 6.0 km with a dominance of small patches (≤0.5 km). Frequency maxima were usually observed at the smaller (≤0.5 km) and larger (≥2.0 km) scales for the nutrients and only at larger (≥2.0 km) scale for salinity. The distribution spectra of patch dimensions were characteristic for each sampling experiment, depending on tidal currents and prevailing wind conditions. Estimated patch dimensions were larger parallel to the current direction than perpendicular to current direction, implying that spatial structures are elongated in the sense of the current direction. Higher winds have, first, a tendency to increase the small–scale structure of the environment by breaking up larger patches into smaller patches, before structures are completely eliminated. The implication of these findings is that different results could be obtained depending on the sampling strategy used (sampling either at anchor stations or at random, independent of current direction), which could lead to different conclusions
Interactions entre les structures d'échappement et les structures à grande échelle dans l'écoulement turbulent des rivières à lit de graviers
Dans les rivières graveleuses, il est établi que les structures d'échappement formées dans la zone de recirculation à l'aval d'amas de galets génèrent d'intenses échanges turbulents. Le mécanisme responsable de l'échappement demeure par contre mal connu. Peu d'études sur la dynamique des structures d'échappement ont été réalisées dans des écoulements où le nombre de Reynolds est élevé comme c'est le cas en rivières. De plus, les connaissances actuelles ne tiennent pas compte des découvertes récentes sur la turbulence en rivière à lit de graviers où on a observé des structures de forte et de faible vitesse occupant toute la profondeur de l'écoulement et pouvant durer plusieurs secondes. Ces structures à grande échelle devraient jouer un rôle sur le mécanisme d'échappement étant donné l'influence de la vitesse ambiante sur la dynamique de la zone de recirculation. Nous rapportons les résultats de deux expériences originales sur les liens dynamiques entre les structures à grande échelle et le mécanisme d'échappement en aval d'un amas de galets. La première expérience repose sur l'analyse de corrélations croisées entre des séries de vitesses obtenues au sommet et à l'aval proximal d'un amas de galets. Les résultats montrent que les fortes fluctuations dans le sens de l'écoulement au sommet de l'obstacle sont liées, quelques instants plus tard, à de fortes fluctuations vers l'amont dans la zone de recirculation. La seconde expérience utilise la visualisation des structures d'échappement et la mesure simultanée des vitesses de l'écoulement. L'analyse combinée des images vidéo et de séries de vitesse suggère une relation entre le passage des structures à grande échelle et les manifestations de l'échappement. Ces résultats nous permettent de présenter un modèle où, lors du passage d'un front de haute vitesse, une structure d'échappement se développe et prend de l'expansion vers le lit et vers la surface en se propageant vers l'aval alors que, lors du passage d'un front de faible vitesse, elle s'élève vers la surface de manière plus cohérente. Cette étude propose un nouveau mécanisme d'échappement et révèle le rôle que joue la structure de l'écoulement ambiant sur le développement de structures dans les cours d'eau à lit graveleux.The flow structure in a gravel-bed river is closely related to the presence of protruding clasts and of pebble clusters. It is well known that shedding motions from the lee side of large clasts and clusters are a recurrent process that explains the strong exchanges of momentum in river flows. However, shedding has yet to be fully characterised for high Reynolds number flows such as those found in gravel-bed rivers. Moreover, our current understanding of shedding mechanisms does not include the recent discovery that large-scale flow structures in the form of high- and low-speed wedges occupy the entire flow depth over a gravel-bed river. From two original experiments, this paper investigates the influence of these wedges on the nature of shedding in the lee of a pebble cluster. The interactions between the large-scale wedges and shedding may be a key element for understanding flow organisation at the river reach scale. The first experiment provides an analysis of the space-time correlation of velocity time series obtained downstream from a pebble cluster in a natural river. Two pairs of one-minute time series were sampled. The first series of each pair was located in the region of flow separation downstream from the obstacle whereas the second was located at its crest. Results show that a significant negative correlation occurs with a negative time lag for the downstream velocity component. This reveals that a strong downstream velocity vector at the crest of the obstacle is followed 1 to 4 seconds later by a strong upstream velocity vector in the region of flow separation. The strength of the recirculation motion responds to the velocity fluctuations above the cluster. This is a crucial process in the development of vortex shedding. The second experiment aimed at visualising the shedding motion downstream from an obstacle. An underwater camera was used to obtain images of fluid motion in the lee of a pebble cluster while three electromagnetic current meters measured streamwise and vertical velocity fluctuations along a vertical profile downstream from the obstacle. A white tracer was injected in the region of flow separation to depict the development of flow structures that are shed into the flow. Despite the high Reynolds number of the flow, we have obtained good quality images revealing the presence of different modes of vortex shedding initiated in the region of flow separation. From the velocity records, it was possible to identify the large-scale flow wedges and to show that the type of vortex shedding is controlled by high- and low-speed wedges.Based on these results, we propose a model having two steps: when a high-speed wedge approaches the pebble cluster, the shedding motion develops vertically both towards the water surface and towards the bed as the structures convect downstream; when a low-speed wedge passes, the shedding motion advects mainly towards the surface and it conserves a stronger coherence. This response of the shedding motion to the type of flow wedge is a recurrent and fundamental phenomenon. The results and the model presented herein shed light on the complex nature of vortex shedding in flows at high Reynolds number such as those found in rivers
Quantitative estimates of sinking sea ice particulate organic carbon based on the biomarker IP<inf>25</inf>
Sea ice-derived particulate organic carbon (iPOC) represents an important contribution of carbon to Arctic ecosystems, yet our ability to obtain realistic quantitative estimates of iPOC outside the sea ice matrix is currently somewhat limited. To address this challenge, we applied a novel approach to quantifying iPOC within the water column under melting sea ice by first measuring the proportion of the sea ice diatom biomarker IP25 within iPOC in bottom ice samples obtained from Resolute Passage during spring 2012. We then compared this value with corresponding values obtained from a time series of water samples. Together, these reflected a period of ice melt and rapid release of iPOC, indicated by changing ice temperature and thickness, in addition to changes in the stable carbon isotope composition and concentration of iPOC, IP25 and chlorophyll a within bottom ice. Estimates of iPOC in seawater were highest (0.15 to 0.22 mg l-1) in the upper 2 m, coincident with the reduction of iPOC in sea ice near the beginning of sampling, with iPOC accounting for an estimated 84 to 125% of total POC (tPOC). Collectively, this biomarker approach yielded realistic estimates of %iPOC, both numerically and in the context of melting sea ice following a spring bloom in the Canadian Arctic. We describe some assumptions of this approach and consider the impacts of possible caveats on quantitative estimates of iPOC derived using this methodology
Near-equivalence of the role of structural unpinning number, basicity and reciprocal average electronegativity in determining the conductivity of glasses
The chemical approach made to investigate the origin of fast ion conduction in Agl-based fast ion conducting (FIC) glasses has been extended to various ionically conducting systems containing Na+ ion. An index known as structural unpinning number (SUN), S, has been defined for this purpose based on the unscreened nuclear charge on the cation and the average electronegativity of all the anions. Variation of the log(conductivity), at a given temperature, as a function of structural unpinning number, optical basicity, λ, and the reciprocal average electronegativity of all the anions, l/χa, has been examined for a number of Na+-ion conducting glasses and a nearly identical variation has been noticed in all the cases. The equivalence of these chemical parameters as determinants of the conductivity behavior of glasses has thus been established and the origin of this equivalence has been discussed
Extraspinal sciatica revealing late metastatic disease from parotid carcinoma
Sciatica is a clinical symptom usually caused by a disk herniation and less often by other conditions such as tumors, infections, or inflammatory diseases. We report the case of a woman in whom sciatica led to the identification of a large pelvic metastasis from a carcinoma of the parotid gland
Implications of sea-ice biogeochemistry for oceanic production and emissions of dimethyl sulfide in the Arctic
Sea ice represents an additional oceanic source of the climatically active gas dimethyl sulfide (DMS) for the Arctic atmosphere. To what extent this source contributes to the dynamics of summertime Arctic clouds is, however, not known due to scarcity of field measurements. In this study, we developed a coupled sea ice–ocean ecosystem–sulfur cycle model to investigate the potential impact of bottom-ice DMS and its precursor dimethylsulfoniopropionate (DMSP) on the oceanic production and emissions of DMS in the Arctic. The results of the 1-D model simulation were compared with field data collected during May and June of 2010 in Resolute Passage. Our results reproduced the accumulation of DMS and DMSP in the bottom ice during the development of an ice algal bloom. The release of these sulfur species took place predominantly during the earlier phase of the melt period, resulting in an increase of DMS and DMSP in the underlying water column prior to the onset of an under-ice phytoplankton bloom. Production and removal rates of processes considered in the model are analyzed to identify the processes dominating the budgets of DMS and DMSP both in the bottom ice and the underlying water column. When openings in the ice were taken into account, the simulated sea–air DMS flux during the melt period was dominated by episodic spikes of up to 8.1 µmol m−2 d−1. Further model simulations were conducted to assess the effects of the incorporation of sea-ice biogeochemistry on DMS production and emissions, as well as the sensitivity of our results to changes of uncertain model parameters of the sea-ice sulfur cycle. The results highlight the importance of taking into account both the sea-ice sulfur cycle and ecosystem in the flux estimates of oceanic DMS near the ice margins and identify key uncertainties in processes and rates that should be better constrained by new observations
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