24 research outputs found

    An example of suppression of spurious stop-bands of EBG band-stop filter

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    Based on the well known theory of infinite periodic structures, analytical theory of EBG (electromagnetic band gap) cells suppressing 6 higher (spurious) stop-bands is developed. Using such cells in a cascade the straight-forward procedure for design of the corresponding EBG band-stop filter is proposed, with possibility to control the width and the depth of the stopband. The analytical theory is confirmed by the EM simulation of the filter realized in the microstrip technology

    Analysis of axially symmetric wire antennas by the use of exact kernel of electric field integral equation

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    The paper presents a new method for the analysis of wire antennas with axial symmetry. Truncated cones have been applied to precisely model antenna geometry, while the exact kernel of the electric field integral equation has been used for computation. Accuracy and efficiency of the method has been further increased by the use of higher order basis functions for current expansion, and by selecting integration methods based on singularity cancelation techniques for the calculation of potential and impedance integrals. The method has been applied to the analysis of a typical dipole antenna, thick dipole antenna and a coaxial line. The obtained results verify the high accuracy of the method. [Projekat Ministarstva nauke Republike Srbije, br. TR-32005

    Complete genome sequence of the cyanophage S-PRM1 isolated from Singapore coastal waters

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    Here, we report the genomic sequence and comparative analysis of the cyanophage S-PRM1 which was recently isolated from Singapore coastal waters using Synechococcus sp. strain WH7803. The genome of 144,311ā€Æbp was sequenced using a combination of Illumina and Oxford Nanopore technologies. The calculated OrthoANI (Average Nucleotide Identity by Orthology) values of S-PRM1 were between 65.24% and 89.39% when compared with previously sequenced cyanomyoviruses with the highest identity with cyanophage S-CAM9. Auxiliary metabolic genes (AMGs) involved in photosynthesis, carbon metabolism, phosphate acquisition, DNA biosynthesis were found in the genome of S-PRM1.NRF (Natl Research Foundation, Sā€™pore)Accepted versio

    RNA viruses in aquatic ecosystems through the lens of ecological genomics and transcriptomics

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    Massive amounts of data from nucleic acid sequencing have changed our perspective about diversity and dynamics of marine viral communities. Here, we summarize recent metatranscriptomic and metaviromic studies targeting predominantly RNA viral communities. The analysis of RNA viromes reaffirms the abundance of lytic (+) ssRNA viruses of the order Picornavirales, but also reveals other (+) ssRNA viruses, including RNA bacteriophages, as important constituents of extracellular RNA viral communities. Sequencing of dsRNA suggests unknown diversity of dsRNA viruses. Environmental metatranscriptomes capture the dynamics of ssDNA, dsDNA, ssRNA, and dsRNA viruses simultaneously, unravelling the full complexity of viral dynamics in the marine environment. RNA viruses are prevalent in large size fractions of environmental metatranscriptomes, actively infect marine unicellular eukaryotes larger than 3 Āµm, and can outnumber bacteriophages during phytoplankton blooms. DNA and RNA viruses change abundance on hourly timescales, implying viral control on a daily temporal basis. Metatranscriptomes of cultured protists host a diverse community of ssRNA and dsRNA viruses, often with multipartite genomes and possibly persistent intracellular lifestyles. We posit that RNA viral communities might be more diverse and complex than formerly anticipated and that the influence they exert on community composition and global carbon flows in aquatic ecosystems may be underestimated.Agency for Science, Technology and Research (A*STAR)National Research Foundation (NRF)Published versionThis work was supported by the National Research Foundation, Primeā€™s Ministerā€™s Office, Singapore under its Intra-CREATE Seed Collaboration Grant (Award NRF2018-ITS004-0001) and Competitive Research Programme (Award CRP21-2018-0005). Sandra Kolundzija is supported by a Singapore International Graduate Award (SINGA) from the Agency for Science, Technology & Research (A*STAR)

    In vitro antitumor activity, metal uptake and reactivity with ascorbic acid and BSA of some gold(III) complexes with N,N '-ethylenediamine bidentate ester ligands

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    Four novel gold(III) complexes of general formulae [AuCl2{(S,S)-R(2)eddl}]PF6 (R(2)eddl = O,O'-dialkyl-(S,S)-ethylenediamine-N,N'-di-2-(4-methyl)pentanoate, R= n-Pr, n-Bu, n-Pe, i-Bu; 1-4, respectively), were synthesized and characterized by elemental analysis, UV/Vis, IR, and NMR spectroscopy, as well as high resolution mass spectrometry. Density functional theory calculations pointed out that (R,R)-N,N'-configuration diastereoisomers were energetically the most favorable. Duo to high cytotoxic activity complex 3 was chosen for stability study in DMSO, no decomposition occurs within 24 h, and for the reaction with ascorbic acid in which was reduced immediately. Additionally, 3 interacts with bovine serum albumin (BSA) as proven by UV/Vis spectroscopy. In vitro antitumor activity was determined against human cervix adenocarcinoma (HeLa), human myelogenous leukemia (K562), and human melanoma (Fem-x) cancer cell lines, as well as against non-cancerous human embryonic lung fibroblast cells MRC-5. The highest activity was observed against K562 cells (IC50: 5.04-6.51 mu M). Selectivity indices showed that these complexes are less toxic than cisplatin. 3 had a similar viability kinetics on HeLa cells as cisplatin. Drug accumulation studies in HeLa cells showed that the total gold uptake increased much faster than that of cisplatin pointing out that 3 more efficiently enters the cells than cisplatin. Furthermore, morphological and cell cycle analysis reveal that gold(III) complexes induced apoptosis in time- and dose-dependent manner.Peer-reviewed manuscript: [http://cherry.chem.bg.ac.rs/handle/123456789/3070]Supplementary material: [http://cherry.chem.bg.ac.rs/handle/123456789/3071
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