30 research outputs found

    Purified F-ATP synthase forms a Ca2+-dependent high-conductance channel matching the mitochondrial permeability transition pore

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    The molecular identity of the mitochondrial megachannel (MMC)/permeability transition pore (PTP), a key effector of cell death, remains controversial. By combining highly purified, fully active bovine F-ATP synthase with preformed liposomes we show that Ca2+ dissipates the H+ gradient generated by ATP hydrolysis. After incorporation of the same preparation into planar lipid bilayers Ca2+ elicits currents matching those of the MMC/PTP. Currents were fully reversible, were stabilized by benzodiazepine 423, a ligand of the OSCP subunit of F-ATP synthase that activates the MMC/PTP, and were inhibited by Mg2+ and adenine nucleotides, which also inhibit the PTP. Channel activity was insensitive to inhibitors of the adenine nucleotide translocase (ANT) and of the voltage-dependent anion channel (VDAC). Native gel-purified oligomers and dimers, but not monomers, gave rise to channel activity. These findings resolve the long-standing mystery of the MMC/PTP and demonstrate that Ca2+ can transform the energy-conserving F-ATP synthase into an energy-dissipating device

    A first measurement of the interaction cross section of the tau neutrino

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    The DONuT experiment collected data in 1997 and published first results in 2000 based on four observed ντ\nu_\tau charged-current (CC) interactions. The final analysis of the data collected in the experiment is presented in this paper, based on 3.6×10173.6 \times 10^{17} protons on target using the 800 GeV Tevatron beam at Fermilab. The number of observed ντ\nu_\tau CC interactions is 9, from a total of 578 observed neutrino interactions. We calculated the energy-independent part of the tau-neutrino CC cross section (ν+νˉ\nu + \bar \nu), relative to the well-known νe\nu_e and νμ\nu_\mu cross sections. The ratio σ(ντ)\sigma(\nu_\tau)/σ(νe,μ)\sigma(\nu_{e,\mu}) was found to be 1.37±0.35±0.771.37\pm0.35\pm0.77. The ντ\nu_\tau CC cross section was found to be 0.72±0.24±0.36×10380.72 \pm 0.24\pm0.36 \times 10^{-38} cm2GeV1^{2}\rm{GeV}^{-1}. Both results are in agreement the Standard Model.Comment: 37 pages, 15 figure
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