102 research outputs found
Stationary rotary force waves on the liquid–air core interface of a swirl atomizer
A one-dimensional wave equation, applicable to the waves on the surface of the air-core of a swirl atomizer is derived analytically, by analogy to the similar one-dimensional wave equation derivation for shallowwater gravity waves. In addition an analogy to the flow of water over a weir is used to produce an analytical derivation of the flow over the lip of the outlet of a swirl atomizer using the principle of maximum flow. The principle of maximum flow is substantiated by reference to continuity of the discharge in the direction of streaming. For shallowwater gravity waves, the phase velocity is the same expression as for the critical velocity over the weir. Similarly, in the present work, the wave phase velocity on the surface of the air-core is shown to be the same expression as for the critical velocity for the flow at the outlet. In addition, this wave phase velocity is shown to be the square root of the product of the radial acceleration and the liquid thickness, as analogous with the wave phase velocity for shallow water gravity waves, which is the square root of the product of the acceleration due to gravity and the water depth. The work revisits the weirs and flumes work of Binnie et al. but using a different methodology. The results corroborate with the work of Binnie. High speed video, Laser Doppler Anemometry and deflected laser beam experimental work has been carried out on an oversize Perspex (Plexiglas) swirl atomizer. Three distinctive types of waves were detected: helical striations, low amplitude random ripples and low frequency stationary waves. It is the latter wave type that is considered further in this article. The experimentally observed waves appear to be stationary upon the axially moving flow. The mathematical analysis allows for the possibility of a negative value for the phase velocity expression. Therefore the critical velocity and the wave phase velocity do indeed lead to stationary waves in the atomizer. A quantitative comparison between the analytically derived wave phase velocity and that measured experimentally, for this stationary pulsating wave, show very good agreement within a few percent
The ter Mutation in the Rat Dnd1 Gene Initiates Gonadal Teratomas and Infertility in Both Genders
A spontaneous mutation leading to the formation of congenital ovarian and testicular tumors was detected in the WKY/Ztm rat strain. The histological evaluation revealed derivatives from all three germ layers, thereby identifying these tumors as teratomas. Teratocarcinogenesis was accompanied by infertility and the underlying mutation was termed ter. Linkage analysis of 58 (WKY-ter×SPRD-Cu3) F2 rats associated the ter mutation with RNO18 (LOD = 3.25). Sequencing of candidate genes detected a point mutation in exon 4 of the dead-end homolog 1 gene (Dnd1), which introduces a premature stop codon assumed to cause a truncation of the Dnd1 protein. Genotyping of the recessive ter mutation revealed a complete penetrance of teratocarcinogenesis and infertility in homozygous ter rats of both genders. Morphologically non-tumorous testes of homozygous ter males were reduced in both size and weight. This testicular malformation was linked to a lack of spermatogenesis using immunohistochemical and histological staining. Our WKY-Dnd1ter/Ztm rat is a novel animal model to investigate gonadal teratocarcinogenesis and the molecular mechanisms involved in germ cell development of both genders
Mouse Apolipoprotein B Editing Complex 3 (APOBEC3) Is Expressed in Germ Cells and Interacts with Dead-End (DND1)
encoded protein, DND1, is able to bind to the 3′-untranslated region (UTR) of messenger RNAs (mRNAs) to displace micro-RNA (miRNA) interaction with mRNA. Thus, one function of DND1 is to prevent miRNA mediated repression of mRNA. We report that DND1 interacts specifically with APOBEC3. APOBEC3 is a multi-functional protein. It inhibits retroviral replication. In addition, recent studies show that APOBEC3 interacts with cellular RNA-binding proteins and to mRNA to inhibit miRNA-mediated repression of mRNA.Here we show that DND1 specifically interacts with another cellular protein, APOBEC3. We present our data which shows that DND1 co-immunoprecipitates APOBEC3 from mammalian cells and also endogenous APOBEC3 from mouse gonads. Whether the two proteins interact directly remains to be elucidated. We show that both DND1 and APOBEC3 are expressed in germ cells and in the early gonads of mouse embryo. Expression of fluorescently-tagged DND1 and APOBEC3 indicate they localize to the cytoplasm and when DND1 and APOBEC3 are expressed together in cells, they sequester near peri-nuclear sites.The 3′-UTR of mRNAs generally encode multiple miRNA binding sites as well as binding sites for a variety of RNA binding proteins. In light of our findings of DND1-APOBEC3 interaction and taking into consideration reports that DND1 and APOBEC3 bind to mRNA to inhibit miRNA mediated repression, our studies implicate a possible role of DND1-APOBEC3 interaction in modulating miRNA-mediated mRNA repression. The interaction of DND1 and APOBEC3 could be one mechanism for maintaining viability of germ cells and for preventing germ cell tumor development
Selection of stable growth conditions for the parabolic dendrite tip in crystallization of multicomponent melts
Evidence for dendritic fragmentation in as-solidified samples of deeply undercooled melts
The congruently melting, single phase intermetallic β-Ni3Ge has been subject to rapid solidification via drop-tube processing. We establish that the rapidly solidified material growing during the recalescence phase of solidification can be distinguished from the post-recalescence material in the as-solidified sample by the degree of chemical ordering displayed. This can in turn be used to visualize the material from the recalescence phase of solidification. At intermediate cooling rates this recalescence material consists of fragmented dendrites. The occurrence of fragmentation is compared against established theoretical models based on the growth of Rayleigh instabilities with excellent agreement being found. EBSD mapping is used to establish the relationship between these dendritic fragments and the final grain size distribution. The dendritic fragments are found to be poor nuclei for new grains and the fragmented dendrites do not consistently give rise to classical grain refined structures
Boundary-Layer Formulation of Dendritic Growth: Existence of a Family of Steady-State Needle Solutions
FWN – Publicaties zonder aanstelling Universiteit Leide
Boundary-layer approaches to dendritic growth
FWN – Publicaties zonder aanstelling Universiteit Leide
Temperature Distribution in a Cylindrical Rod Moving From a Chamber at One Temperature to a Chamber at Another Temperature
Determination of symmetric end-loadings from the bulk stress on the lateral surface of a rectangular prism
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