Landslide monitoring by fixed-base terrestrial stereo-photogrammetry

Photogrammetry has been used since long to periodically control the evolution of landslides; however, true monitoring is reserved to robotic total stations and ground based InSAR systems, capable of high frequency, high accurate 24h/day response. This paper presents the first results of a fixed terrestrial stereo photogrammetric system developed to monitor shape changes of the scene. The system is made of two reflex cameras, each contained in a sealed box with a control computer that periodically acquires an image and send it to a host computer; once an image pair is received from the two cameras, the DSM of the scene is generated by image correlation and made available for archiving or analysis. The system has been installed and is being tested on the Mont de la Saxe landslide, where several monitoring system are active. Some instability of the camera attitude has been noticed and is corrected with an automated procedure. First comparisons with InSAR data show a good agreement

MULTI-TEMPORAL IMAGE CO-REGISTRATION OF UAV BLOCKS: A COMPARISON OF DIFFERENT APPROACHES

Abstract. Traditionally, data co-registration of survey epochs in photogrammetry relied on Ground Control Points (GCP) to keep the reference system unchanged. In the last years, Unmanned Aerial Systems (UAV) are increasingly used in photogrammetric environmental monitoring. The diffusion of affordable UAV platforms equipped with GNSS (Global Navigation Satellite System) centimetre-grade receivers might reduce, but not eliminate, the need for GCP. Conversely, if GNSS-assisted orientation cannot be used or if additional ground control and reliability checks are required, alternatives to repeated GCP survey have been proposed, taking advantage of Structure from Motion (SfM) photogrammetry. In particular, co-registering different epochs image blocks together, identifying corresponding features, has been demonstrated as a viable and efficient approach. In this paper four different strategies easily implementable in a generic commercial photogrammetric software are presented and compared considering three different test sites in Italy subject to different amounts of environmental changes. The influence of the amount and distribution of inter-epoch corresponding points on the accuracy of the reconstruction is investigated. The results show that some of the tested strategies obtains very good results and can be used (although not needed) also in RTK centimetre-grade UAV surveys, leveraging the additional information coming from previous epochs survey to actually increase the survey accuracy and reliability

Evaluation of cytological diagnostic accuracy for canine splenic neoplasms : an investigation in 78 cases using STARD guidelines

Cytology represents a useful diagnostic tool in the preliminary clinical approach to canine splenic lesions, and may prevent unnecessary splenectomy. However, few studies have evaluated diagnostic accuracy of cytology in the diagnosis of canine splenic neoplasms. The aim of this study was to determine overall accuracy, sensitivity, specificity, positive and negative predictive values (i.e. diagnostic accuracy indexes) of cytology for canine splenic neoplasms following Standards for the Reporting of Diagnostic Accuracy Studies (STARD) guidelines. A consecutive series of canine splenic cytological samples was retrospectively retrieved from the database of the Diagnostic Pathology Service of the Department of Veterinary Medicine (DIMEVET\u2014University of Milan). Histopathology was set as the diagnostic reference standard. Cytological cases were enrolled when slides were available for review and when the same lesion was submitted for histopathology. Seventy-eight (78) lesions were included in the study. By histopathology, 56 were neoplastic and 22 were non-neoplastic. Cytology had an overall accuracy of 73.08% (95% C.I. 61.84%-82.50%), sensitivity of 64.29% (95% C.I. 50.36%-76.64%), specificity of 95.45% (95% C.I. 77.16%-99.88%), and positive and negative predictive values of 97.3% (95% C.I. 84.01%-99.60%) and 51.22% (95% C.I. 42.21%-60.15%), respectively. Low sensitivity and negative predictive value were balanced by very high specificity and positive predictive value. When positive for neoplasia, cytology represents a useful diagnostic tool to rule in splenic neoplasia, prompting surgery independently from other diagnostic tests. Conversely, a negative cytological result requires additional investigations to confirm the dog to be disease free

A Panel of Eight miRNAs Is Deregulated in HTLV-2 Infected PBMCs and BJABGu Cell Line

Despite human T-cell leukemia virus type 1 (HTLV-1) and HTLV-2 being retroviruses closely related at a genomic level, HTLV-2 differs from HTLV-1 in terms of pathogenicity in both single infection and coinfection contexts. Moreover, the HTLV-2 association with clinical outcomes is still debated and several mechanisms underlying HTLV-2 infection remain unexplored as well. Cellular miRNAs are key factors in the post-transcriptional regulation of gene expression and they are known to be potential targets for several pathogens to control the host microenvironment and, in particular, escape immune responses. Here, we identified a HTLV-2-related signature of eight miRNAs (miR-125a-3p, miR-381-3p, miR-502-5p, miR-708-5p, miR-548d-5p, miR-548c-5p, miR-1-3p, and miR-511-5p) in both HTLV-2 infected PBMC and BJABGu cell lines. Altered miRNA expression patterns were correlated with the impairment of Th cell differentiation and signaling pathways driven by cytokines and transcriptional factors such as the Runt-related transcription factor (RUNX) family members. Specifically, we demonstrated that the RUNX2 protein was significantly more expressed in the presence of Tax-2 compared with Tax-1 in an in vitro cell model. To the best of our knowledge, these data represent the first contribution to elucidating the HTLV-2 mediated alteration of host cell miRNA profiles that may impact on HTLV-2 replication and persistent infection

Non-lethal effects of N-acetylcysteine on xylella fastidiosa strain De Donno biofilm formation and detachment

This study investigated in-vitro the non-lethal eects of N-acetylcysteine (NAC) on Xylella fastidiosa subspecies pauca strain De Donno (Xf-DD) biofilm. This strain was isolated fromthe olive trees aected by the olive quick decline syndrome in southern Italy. Xf-DD was first exposed to non-lethal concentrations of NAC from 0.05 to 1000 M. Cell surface adhesion was dramatically reduced at 500 M NAC (47%), hence, this concentration was selected for investigating the eects of pre-, postand co-treatments on biofilm physiology and structural development, oxidative homeostasis, and biofilm detachment. Even though 500 MNAC reduced bacterial attachment to surfaces, compared to the control samples, it promoted Xf-DD biofilm formation by increasing: (i) biofilm biomass by up to 78% in the co-treatment, (ii) matrix polysaccharides production by up to 72% in the pre-treatment, and (iii) reactive oxygen species levels by 3.5-fold in the co-treatment. Xf-DD biofilm detachment without and with NAC was also investigated. The NAC treatment did not increase biofilm detachment, compared to the control samples. All these findings suggested that, at 500 M, NAC diversified the phenotypes in Xf-DD biofilm, promoting biofilm formation (hyper-biofilm-forming phenotype) and discouraging biofilm detachment (hyper-attachment phenotype), while increasing oxidative stress level in the biofilm

USE OF UAS IN A HIGH MOUNTAIN LANDSCAPE: THE CASE OF GRAN SOMMETTA ROCK GLACIER (AO)

Photogrammetry has been used since long time to periodically control the evolution of landslides, either from aerial images as well as from ground. Landslides control and monitoring systems face a large variety of cases and situations: in hardly accessible environments, like glacial areas and high mountain locations, it is not simple finding a survey method and a measurement control system, which are capable to reliably assess, with low costs, the expected displacement and its accuracy. For this reason, the behaviour of these events presents the geologists and the surveyor each time with different challenges. The use of UAS (Unmanned Aerial System) represents, in this context, a recent and valid option to perform the data acquisition both in safety and quickly, avoiding hazards and risks for the operators while at the same time containing the costs. The paper presents an innovative monitoring system based on UAS-photogrammetry, GNSS survey and DSM change detection techniques to evaluate the Gran Sommetta rock glacier surface movements over the period 2012-2014. Since 2012, the surface movements of the glacier are monitored by ARPAVdA (a regional environmental protection agency) as a case study for the impact of climate change on high-mountain infrastructures. In such scenarios, in fact, a low-cost monitoring activity can provide important data to improve our knowledge about glacier dynamics connected to climate changes and to prevent risks in anthropic Alps areas. To evaluate the displacements of the rock glacier different techniques were proposed: the most reliable uses the orthophoto of the area and rely on a manual identification of corresponding features performed by a trained operator. To further limit the costs and improve the density of displacement information two automatic procedures were developed as well

Landslide monitoring by fixed-base terrestrial stereo-photogrammetry

Photogrammetry has been used since long to periodically control the evolution of landslides; however, true monitoring is reserved to robotic total stations and ground based InSAR systems, capable of high frequency, high accurate 24h/day response. This paper presents the first results of a fixed terrestrial stereo photogrammetric system developed to monitor shape changes of the scene. The system is made of two reflex cameras, each contained in a sealed box with a control computer that periodically acquires an image and send it to a host computer; once an image pair is received from the two cameras, the DSM of the scene is generated by image correlation and made available for archiving or analysis. The system has been installed and is being tested on the Mont de la Saxe landslide, where several monitoring system are active. Some instability of the camera attitude has been noticed and is corrected with an automated procedure. First comparisons with InSAR data show a good agreement