1,517 research outputs found
Alternatively spliced exons of the beta tropomyosin gene exhibit different affinities for F-actin and effects with nonmuscle caldesmon
The rat beta-tropomyosin (TM) gene expresses two isoforms via alternative RNA splicing, namely skeletal muscle beta-TM and fibroblast TM-1. The latter is also expressed in smooth muscle where it corresponds to smooth muscle beta-TM. Skeletal muscle beta-TM contains exons 7 and 10, whereas exons 6 and 11 are used in fibroblasts and smooth muscle. In order to study the properties of the alternatively spliced proteins, recombinant TMs derived from bacterial and insect cell expression systems were produced, including the normal beta gene products, fibroblast TM-1 and beta skeletal muscle TM, two carboxy-terminal chimeric TMs, TM-6/10 and TM-7/11, as well as a carboxyl-truncated version of each, TM-6Cla and TM-7Cla. The purified TM isoforms were used in actin filament association studies. The apparent TM association constants (Ka) were taken as the free concentration at half saturation and were found to be 6 microM for beta Sk TM, 8.5 for TM-6/10, 25 microM for TM-1, and 30 microM for TM-7/11 at an F-actin concentration of 42 microM. For the truncated TMs, the values determined were higher still but the binding was not carried out to full saturation. Isoforms were also produced using the baculovirus-insect cell system which produces proteins with an acetylated amino terminus as is normally found in vivo. This modification significantly enhanced the F-actin association of TM-1 but not the beta skeletal TM or the other isoforms. Fibroblast TM-2 or TM-3, both products of the alpha gene, enhanced the affinity of TM-1 for F-actin, demonstrating different isoforms can act cooperatively on binding to actin. This effect was not detected with the other expressed beta gene products. The presence of 83 kDa nonmuscle caldesmon was found to enhance the binding of TM-1 for F-actin. This effect was dependent on the presence of both exons 6 and 11, as caldesmon had little effect on the other beta gene products. Collectively these results demonstrate TMs differ in their affinity for F-actin, which can be altered by other TMs or actin-binding proteins. The beta tropomyosin isoforms were fluorescently-tagged and microinjected into cultured cells to study their in vivo localization where it was found that each of the full-length TMs bound to microfilaments but, at the light microscopy level, the isoforms were not differentially localized in these fibroblasts
MIDAS, prototype Multivariate Interactive Digital Analysis System for large area earth resources surveys. Volume 1: System description
A third-generation, fast, low cost, multispectral recognition system (MIDAS) able to keep pace with the large quantity and high rates of data acquisition from large regions with present and projected sensots is described. The program can process a complete ERTS frame in forty seconds and provide a color map of sixteen constituent categories in a few minutes. A principle objective of the MIDAS program is to provide a system well interfaced with the human operator and thus to obtain large overall reductions in turn-around time and significant gains in throughput. The hardware and software generated in the overall program is described. The system contains a midi-computer to control the various high speed processing elements in the data path, a preprocessor to condition data, and a classifier which implements an all digital prototype multivariate Gaussian maximum likelihood or a Bayesian decision algorithm. Sufficient software was developed to perform signature extraction, control the preprocessor, compute classifier coefficients, control the classifier operation, operate the color display and printer, and diagnose operation
MIDAS, prototype Multivariate Interactive Digital Analysis System, Phase 1. Volume 2: Diagnostic system
The MIDAS System is a third-generation, fast, multispectral recognition system able to keep pace with the large quantity and high rates of data acquisition from present and projected sensors. A principal objective of the MIDAS Program is to provide a system well interfaced with the human operator and thus to obtain large overall reductions in turn-around time and significant gains in throughout. The hardware and software generated in Phase I of the over-all program are described. The system contains a mini-computer to control the various high-speed processing elements in the data path and a classifier which implements an all-digital prototype multivariate-Gaussian maximum likelihood decision algorithm operating 2 x 105 pixels/sec. Sufficient hardware was developed to perform signature extraction from computer-compatible tapes, compute classifier coefficients, control the classifier operation, and diagnose operation. Diagnostic programs used to test MIDAS' operations are presented
Methodology for designing accelerated aging tests for predicting life of photovoltaic arrays
A methodology for designing aging tests in which life prediction was paramount was developed. The methodology builds upon experience with regard to aging behavior in those material classes which are expected to be utilized as encapsulant elements, viz., glasses and polymers, and upon experience with the design of aging tests. The experiences were reviewed, and results are discussed in detail
TeV Gamma Rays from Geminga and the Origin of the GeV Positron Excess
The Geminga pulsar has long been one of the most intriguing MeV-GeV gamma-ray
point sources. We examine the implications of the recent Milagro detection of
extended, multi-TeV gamma-ray emission from Geminga, finding that this reveals
the existence of an ancient, powerful cosmic-ray accelerator that can plausibly
account for the multi-GeV positron excess that has evaded explanation. We
explore a number of testable predictions for gamma-ray and electron/positron
experiments (up to ~100 TeV) that can confirm the first "direct" detection of a
cosmic-ray source.Comment: 4 pages and 3 figures; Minor revisions, accepted for publication in
Physical Review Letter
A Survey About Nothing: Monitoring a Million Supergiants for Failed Supernovae
Extragalactic transient searches have historically been limited to looking
for the appearance of new sources such as supernovae. It is now possible to
carry out a new kind of survey that will do the opposite, that is, search for
the disappearance of massive stars. This will entail the systematic observation
of galaxies within a distance of 10 Mpc in order to watch ~10^6 supergiants.
Reaching this critical number ensures that something will occur yearly, since
these massive stars must end their lives with a core collapse within ~10^6
years. Using deep imaging and image subtraction it is possible to determine the
fates of these stars whether they end with a bang (supernova) or a whimper
(fall out of sight). Such a survey would place completely new limits on the
total rate of all core collapses, which is critical for determining the
validity of supernova models. It would also determine the properties of
supernova progenitors, better characterize poorly understood optical
transients, such as eta Carina-like mass ejections, find and characterize large
numbers of Cepheids, luminous blue variables and eclipsing binaries, and allow
the discovery of any new phenomena that inhabit this relatively unexplored
parameter space.Comment: final version, 7 pages, 5 figures, ApJ in pres
Gamma-ray signatures of annihilation to charged leptons in dark matter substructure
Due to their higher concentrations and small internal velocities, Milky Way
subhalos can be at least as important as the smooth halo in accounting for the
GeV positron excess via dark matter annihilation. After showing how this can be
achieved in various scenarios, including in Sommerfeld models, we demonstrate
that, in this case, the diffuse inverse-Compton emission resulting from
electrons and positrons produced in substructure leads to a nearly-isotropic
signal close to the level of the isotropic GeV gamma-ray background seen by
Fermi. Moreover, we show that HESS cosmic-ray electron measurements can be used
to constrain multi-TeV internal bremsstrahlung gamma rays arising from
annihilation to charged leptons.Comment: 8 pages, 4 figures; minor updates to match published versio
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