704 research outputs found
The Pierre Auger Observatory: Results on Ultra-High Energy Cosmic Rays
The focus of this article is on recent results on ultra-high energy cosmic
rays obtained with the Pierre Auger Observatory. The world's largest instrument
of this type and its performance are described. The observations presented here
include the energy spectrum, the primary particle composition, limits on the
fluxes of photons and neutrinos and a discussion of the anisotropic
distribution of the arrival directions of the most energetic particles.
Finally, plans for the construction of a Northern Auger Observatory in
Colorado, USA, are discussed.Comment: Proceedings of the International Workshop on Advances in Cosmic Ray
Science, Waseda University, Shinjuku, Tokyo, Japan, March 2008; to be
published in the Journal of the Physical Society of Japan (JPSJ) supplemen
THUMP from archaeal tRNA:m(2)(2)G10 methyltransferase, a genuine autonomously folding domain
The tRNA:m(2)(2)G10 methyltransferase of Pyrococus abyssi (PAB1283, a member of COG1041) catalyzes the N(2),N(2)-dimethylation of guanosine at position 10 in tRNA. Boundaries of its THUMP (THioUridine synthases, RNA Methyltransferases and Pseudo-uridine synthases)—containing N-terminal domain [1–152] and C-terminal catalytic domain [157–329] were assessed by trypsin limited proteolysis. An inter-domain flexible region of at least six residues was revealed. The N-terminal domain was then produced as a standalone protein (THUMPα) and further characterized. This autonomously folded unit exhibits very low affinity for tRNA. Using protein fold-recognition (FR) methods, we identified the similarity between THUMPα and a putative RNA-recognition module observed in the crystal structure of another THUMP-containing protein (ThiI thiolase of Bacillus anthracis). A comparative model of THUMPα structure was generated, which fulfills experimentally defined restraints, i.e. chemical modification of surface exposed residues assessed by mass spectrometry, and identification of an intramolecular disulfide bridge. A model of the whole PAB1283 enzyme docked onto its tRNA(Asp) substrate suggests that the THUMP module specifically takes support on the co-axially stacked helices of T-arm and acceptor stem of tRNA and, together with the catalytic domain, screw-clamp structured tRNA. We propose that this mode of interactions may be common to other THUMP-containing enzymes that specifically modify nucleotides in the 3D-core of tRNA
Next-Generation Proteomics Reveals a Greater Antioxidative Response to Drought in Coffea arabica Than in Coffea canephora
Drought is a major threat to coffee, compromising the quality and quantity of its production.
We have analyzed the core proteome of 18 Coffea canephora cv. Conilon Clone 153 and C. arabica
cv. Icatu plants and assessed their responses to moderate (MWD) and severe (SWD) water deficits.
Label-free quantitative shotgun proteomics identified 3000 proteins in both genotypes, but less than
0.8% contributed to ca. 20% of proteome biomass. Proteomic changes were dependent on the severity
of drought, being stronger under SWD and with an enrolment of different proteins, functions, and
pathways than under MWD. The two genotypes displayed stress-responsive proteins under SWD,
but only C. arabica showed a higher abundance of proteins involved in antioxidant detoxification
activities. Overall, the impact of MWD was minor in the two genotypes, contrary to previous
studies. In contrast, an extensive proteomic response was found under SWD, with C. arabica having
a greater potential for acclimation/resilience than C. canephora. This is likely supported by a wider
antioxidative response and an ability to repair photosynthetic structures, being crucial to develop
new elite genotypes that assure coffee supply under water scarcity levelsinfo:eu-repo/semantics/publishedVersio
Final results of the EDELWEISS-II WIMP search using a 4-kg array of cryogenic germanium detectors with interleaved electrodes
The EDELWEISS-II collaboration has completed a direct search for WIMP dark
matter with an array of ten 400-g cryogenic germanium detectors in operation at
the Laboratoire Souterrain de Modane. The combined use of thermal phonon
sensors and charge collection electrodes with an interleaved geometry enables
the efficient rejection of gamma-induced radioactivity as well as near-surface
interactions. A total effective exposure of 384 kg.d has been achieved, mostly
coming from fourteen months of continuous operation. Five nuclear recoil
candidates are observed above 20 keV, while the estimated background is 3.0
events. The result is interpreted in terms of limits on the cross-section of
spin-independent interactions of WIMPs and nucleons. A cross-section of
4.4x10^-8 pb is excluded at 90%CL for a WIMP mass of 85 GeV. New constraints
are also set on models where the WIMP-nucleon scattering is inelastic.Comment: 23 pages, 5 figures; matches published versio
A search for low-mass WIMPs with EDELWEISS-II heat-and-ionization detectors
We report on a search for low-energy (E < 20 keV) WIMP-induced nuclear
recoils using data collected in 2009 - 2010 by EDELWEISS from four germanium
detectors equipped with thermal sensors and an electrode design (ID) which
allows to efficiently reject several sources of background. The data indicate
no evidence for an exponential distribution of low-energy nuclear recoils that
could be attributed to WIMP elastic scattering after an exposure of 113 kg.d.
For WIMPs of mass 10 GeV, the observation of one event in the WIMP search
region results in a 90% CL limit of 1.0x10^-5 pb on the spin-independent
WIMP-nucleon scattering cross-section, which constrains the parameter space
associated with the findings reported by the CoGeNT, DAMA and CRESST
experiments.Comment: PRD rapid communication accepte
Axion searches with the EDELWEISS-II experiment
We present new constraints on the couplings of axions and more generic
axion-like particles using data from the EDELWEISS-II experiment. The EDELWEISS
experiment, located at the Underground Laboratory of Modane, primarily aims at
the direct detection of WIMPs using germanium bolometers. It is also sensitive
to the low-energy electron recoils that would be induced by solar or dark
matter axions. Using a total exposure of up to 448 kg.d, we searched for
axion-induced electron recoils down to 2.5 keV within four scenarios involving
different hypotheses on the origin and couplings of axions. We set a 95% CL
limit on the coupling to photons GeV in
a mass range not fully covered by axion helioscopes. We also constrain the
coupling to electrons, , similar to the more
indirect solar neutrino bound. Finally we place a limit on , where is the
effective axion-nucleon coupling for Fe. Combining these results we
fully exclude the mass range keV for DFSZ axions and
keV for KSVZ axions
- …