Identification of host genes potentially implicated in the Malus pumila and ‘Candidatus Phytoplasma mali’ interactions

Two‘Candidatus Phytoplasma mali’ strains (AP and AT), were studied in experimentally infected apple trees to analyze transcriptional profiles during interaction with phytoplasmas. Three groups of sample combinations were employed: healthy - infected, symptomatic - non-symptomatic, and AP-infected - AT-infected sample. The majority of genes were differently expressed between healthy and infected samples. Changes in gene expression involved a wide spectrum of biological functions, including processes of metabolism, cell defence, photosynthesis, transport, transcription, signal transduction and protein synthesis. The possible effect of phytoplasma infection on these processes and their relationships with disease development, symptom appearance and possible plant defence system is discussed. Keywords: Apple, phytoplasmas, ‘Ca. P. mali’, gene expression, transcriptom

Preliminary evaluation of antimicrobial activity of some chemicals on in vitro apple shoots infected by 'Candidatus Phytoplasma mali'.

peer reviewe

Development Of A New Probe For Specific And Sensitive Detection Of 'Candidatus Phytoplasma Mali' In Inoculated Apple Trees

peer reviewe

Adaptation of real-time PCR assay for specific detection of apple proliferation phytoplasma.

peer reviewe

Preliminary study on some ornamental plant phytoplasma diseases in north of Iran

Because of the importance of ornamental plants culture in north of Iran, and the outbreak of suspicious symptoms of phytoplasma diseases on these plants, a study was carried out in order to detect and identify phytoplasmas associated with them. The presence of phytoplasma in samples of cockscomb, tagetes and rose plants was shown by direct and nested-PCR assays using the phytoplasma-specific primers, P1/P7 and R16F2n/R2. Sequences of cockscomb, Indian marigold and rose phytoplasmas have high homology with the members of aster yellows phytoplasma group (16SrI). Phylogenetic analysis showed that cockscomb, tagetes and rose phytoplasmas are closely related to 16SrI-M the first one and 16SrI-B the latter two, respectively. To our knowledge, this is the first report of a member of 16SrI group phytoplasma infection in cockscomb in Iran. Moreover, the present study is the first report of phytoplasma disease outbreak on rose in north of Iran by subgroup classification of the associated phytoplasma