10 research outputs found
Análisis Numérico del Efecto de la Termoforesis para la Separación de Diferentes Poblaciones de Exosomas
Los sistemas microfluídicos han demostrado ser una alternativa prometedora ante procesos convencionales de separación y caracterización. Este trabajo muestra el estudio numérico del fenómeno de la termoforesis en un microdispositivo para la separación de diferentes poblaciones de exosomas (vesículas entre 40-250nm liberadas por la mayoría de las células) en base a su tamaño. Empleando el software ANSYS Fluent v.16.0 se han analizado diferentes modelos geométricos del microdispositivo, gradientes de temperatura entre la pared superior e inferior y flujos de entrada, con el fin de determinar la trayectoria de los exosomas a través del dispositivo microfluídico y extraer las diferentes poblaciones. En base a los resultados obtenidos se muestra el dispositivo propuesto para la fabricación de un dispositivo eficiente
Reaction Mechanism and Chemoselectivity of Intermolecular Cycloaddition Reactions between Phenyl-Substituted Cyclopropenone Ketal and Methyl Vinyl Ketone
Microfluidic separation processes using the thermodiffusion effect
The numerical and experimental results presented in this work show that a relatively small temperature gradient
(5K), has great potential to generate a separation in interesting mixtures for biological purpose. In fact, it improves molecular diffusion separation process of a protective cryo (10% of dimethyl sulfoxide (DMSO) in phosphate buffered saline (PBS)) for cryopreserved cells, by 10%. This separation process was analyzed both numerically and experimentally, for which it was necessary to determine experimentally the thermophysical and transport properties of the mixture of 10% of DMSO in PBS. Experimental tests were done in a microdevice with a working section of 500μ m × 25 mm and a length of 75 mm, which was designed, constructed and first of all validated with the mixture of reference H2O-Isopropanol at a mass fraction of 50%. Experimental test with the protective cryo were done with in flow rates between 1000μL/min and 4000μL/min. The results confirm that the effect of thermodiffusion must be considered in handling processes of biological fluid mixtures because the
existence of a thermal gradient could improve the efficiency of the separation process in microdevices
Al(H2PO4)3 as an efficient and reusable catalyst for the multi-component synthesis of highly functionalized piperidines and dihydro-2-oxypyrroles
An experimental and numerical investigation of the effect of macro-textured tool surfaces in hot stamping
Fluorescent bicolour sensor for low-background neutrinoless double β decay experiments
Observation of the neutrinoless double β decay is the only practical way to establish that neutrinos are their own antiparticles. Because of the small masses of neutrinos, the lifetime of neutrinoless double β decay is expected to be at least ten orders of magnitude greater than the typical lifetimes of natural radioactive chains, which can mimic the experimental signature of neutrinoless double β decay. The most robust identification of neutrinoless double β decay requires the definition of a signature signal—such as the observation of the daughter atom in the decay—that cannot be generated by radioactive backgrounds, as well as excellent energy resolution. In particular, the neutrinoless double β decay of Xe could be established by detecting the daughter atom, Ba, in its doubly ionized state. Here we demonstrate an important step towards a ‘barium-tagging’ experiment, which identifies double β decay through the detection of a single Ba ion. We propose a fluorescent bicolour indicator as the core of a sensor that can detect single Ba ions in a high-pressure xenon gas detector. In a sensor made of a monolayer of such indicators, the Ba dication would be captured by one of the molecules and generate a Ba-coordinated species with distinct photophysical properties. The presence of such a single Ba-coordinated indicator would be revealed by its response to repeated interrogation with a laser system, enabling the development of a sensor able to detect single Ba ions in high-pressure xenon gas detectors for barium-tagging experiments.We also acknowledge support from the following agencies and institutions: the European Research Council (ERC) under Advanced Grant 339787-NEXT; the Ministry of Science and Innovation of Spain and FEDER under grants FIS2014-53371-C04, FIS2016-76163-R, MAT2016-78293-C6-5-R, MINECO/FEDER CT2016-80955-P, CTQ2016-80375-P and CTQ2014-51912-REDC; Interred PCTEFA V-A Spain/France/Andorra Program (EFA
194/16/TNSI); the Basque Government (GV/EJ) under grants IT-1346-19 and IT-1180-19; andAgencia de Ciencia y Tecnología de la Región de Murcia (19897/GERM/15). The authors also thank the SGI/IZO-SGIker UPV/EHU, Fundación Séneca and DIPC for computational and analytical resources