Avaliação da influência do flunixin meglumine sobre os níveis séricos de progesterona em novilhas e vacas mestiças.

Edição dos resumos da 20. Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Araxá, MG, agosto 2006

Avaliação de diferentes métodos de colheita de embriões bovinos.

Edição dos resumos da 20. Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Araxá, MG, agosto 2006

Targeted replacement of mouse apolipoprotein A-I with human ApoA-I or the mutant ApoA-IMilano. Evidence of APOA-IM impaired hepatic secretion

Despite a pro-atherogenic profile, individuals carrying the molecular variant (R173C) of apolipoprotein (apo)A-I, named apoA-I(Milano) (apoA-I(M)), appear to be at reduced risk for cardiovascular disease. To develop an in vivo system to explore, in a controlled manner, the effects of apoA-I(M) on lipid metabolism, we have used the gene targeting technology, or "gene knock-in" (gene k-in), to replace the murine apoA-I gene with either human apoA-I or apoA-I(M) genes in embryonic stem cells. As in human carriers, mice expressing apoA-I(M) (A-I(M) k-in) are characterized by low concentrations of the human apolipoprotein and reduced high density lipoprotein cholesterol levels, compared with A-I k-in animals. The aim of the present study was to investigate the basic mechanisms of hypoalphalipoproteinemia associated with the apoA-I(M) mutation. ApoA-I and apoA-I(M) mRNA expression, as assessed by Northern blot analysis and quantitative real time reverse transcription-PCR, did not exhibit significant differences in either liver or intestine. Moreover, human apolipoprotein synthesis rates were similar in the k-in lines. When the secretion rate of the human apolipoproteins was assessed in cultured hepatocytes from the mouse lines, secretion from apoA-I(M)-expressing cells was markedly reduced (42% for A-I(M) k-in and 36% for A-I/A-I(M) k-in mice) as compared with that of A-I k-in hepatocytes. These results provide the first evidence that the hypoalphalipoproteinemia in apoA-I(M) human carriers may be partially explained by impaired apoA-I(M) secretion

Avaliação dos efeitos do flunixim meglumine sobre a concentração sérica de progesterona e ciclo estral em novilhas e vacas mestiças.

Resumo: Baseando-se nos mecanismos de interrupção da síntese de PGF2a em bovinos, objetivou-se com este estudo avaliar os efeitos do flunixin meglumine (FM) sobre o comprimento do ciclo estral e sobre os níveis de progesterona, em novilhas e vacas mestiças. Após sincronização dos estros, os animais foram divididos em grupos: controle (cinco vacas e quatro novilhas) e tratado (seis vacas e seis novilhas), sendo submetidos à colheita diária de sangue, até o estro subseqüente. Do 13o ao 18o dia, aplicou-se nos animais do grupo tratado 1,65 mg/kg intramuscular de FM, e nos do grupo controle, mesmo volume de solução fisiológica. A determinação da concentração sérica de progesterona foi realizada por RIA. Os resultados do comprimento do ciclo estral foram comparados entre os grupos e entre animais, pelo teste t de Student, enquanto a concentração de progesterona foi normalizada em relação à luteólise, e analisada entre os grupos pelo teste t de Tukey (SAS). Não se observou diferença (P>0,05) no comprimento do ciclo estral entre grupos e entre novilhas e vacas, tratadas e não tratadas. O dia do ciclo estral em relação à concentração de progesterona máxima e inferior a 1,0ng/mL, e a concentração sérica de progesterona entre os animais do grupo controle e tratado, também foram semelhantes entre os grupos (P>0,05). No entanto, observou-se diferença (P0.05). However, there was no difference concerning the concentration of progesterone decrease among theexperimental groups. It was concluded that, under the conditions this study was carried out, even though the effect of the treatment was observed, its action would be better characterized if the sample population were bigger.(AU

Improved Control Strategy of 2-Sliding Controls Applied to a Flexible Robot Arm

International audienceControlling a flexible robot arm driven by McKibben artificial muscles with direct transmission is delicate. The usual PID controller rapidly reveals itself to be inadequate and robust control tools are unavoidable. Classical sliding control, although robust, generates chatter. Several solutions are available to attenuate this phenomenon, among them the twisting and super twisting algorithms, which belong to the 2-sliding control set. It will be shown when to use the equivalent control and the effect of a noised sensor signal on control performance. Also, the use of an additional discontinuous term that increases robustness, performance and stability is put forward. Experimental results are presented and discussed

An Interdisciplinary Capstone Design Experience on Critical Embedded Systems using Agile Methods

International audienceThis paper relates a capstone design project conceived through an engineering problem-based learning approach. The project is applied to critical embedded systems and the Scrum Agile method is used. The focus of the paper is on the pedagogical experience, so the Intended Learning Outcomes and pedagogical sequence over one semester are presented. Two platforms (the Parrot AR.Drone 2 and a toy car) were used for the project. Pros and cons of such platforms are debated. Tools like Git for the version control and iceScrum for the Agile management are presented. The different roles of teachers as clients, counselors, and evaluators are detailed. This paper shows that an interdisciplinary teaching team is very important in this kind of project, so is the teachers' engagement in the Agile philosophy. Feedback, analyses and some suggested improvements are given, based on our 5 years' experience in such projects and surveys.Cet article relate notre expérience de mise en oeuvre d’un apprentissagebasé sur la résolution de problèmes d’ingénierie au travers d’unprojet interdisciplinaire. Ce projet a pour domaine d’application lessystèmes embarqués critiques. La méthode agile Scrum est utiliséecomme méthode de gestion de projet. L’article porte principalement sur notre expérience pédagogique : il présente les acquis d’apprentissagevisés et la séquence pédagogique tout au long du semestre.Deux plates-formes (le Parrot AR.Drone 2 et une voiturette pour enfants)ont été successivement utilisées pour ce projet. Les avantageset les inconvénients des deux plates-formes sont débattus. Des outilstels que Git pour le contrôle de version et iceScrum pour la gestionagile sont présentés. Les différents rôles des enseignants en tantque clients, conseillers et évaluateurs sont détaillés. Cet article montrequ’une équipe pédagogique interdisciplinaire est très importante dansce type de projet, de même que l’engagement des enseignants dans laphilosophie Agile. L’article donne un retour d’expériences, des analysessur les résultats obtenus, des suggestions d’amélioration sur la base denos cinq années d’expérience et sur des sondages effectués auprès desdifférentes cohortes d’étudiants.Mots-clés : projet interdisciplinaire, systèmes embarqués, méthode agile,apprentissage par résolution de problèmes d’ingénieri

Alterações morfológicas nos órgãos genitais de cães e gatos provenientes de vilas rurais na região de Umuarama, PR.

Edição dos resumos da 20. Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Araxá, MG, agosto 2006

The GLP-1 analog liraglutide attenuates acute liver injury in mice

Introduction and objectives: Acute liver injury is a current health problem with few effective treatments. The present study investigated the hepatoprotective and curative potential of the glucagon-like peptide-1 analog liraglutide against carbon tetrachloride (CCl4)-induced hepatotoxicity. Materials and methods: Male Swiss mice were subjected to two protocols. The first protocol (Pretreatment) consisted of intraperitoneal (i.p.) treatment with liraglutide (0.057 and 0.118 mg kg−1) or vehicle (distilled water) once daily for 7 days. On days 6 and 7, the animals were challenged with 2% CCl4 (5 mg kg−1, i.p.). The second protocol (Late treatment) began with an injection of 5% CCl4 (5 mg kg−1, i.p.) and subsequent treatment with liraglutide (0.057 mg kg−1) or vehicle (distilled water) for 1 day. In both protocols, 24 h after the last administration, blood and bile were collected from anesthetized animals, followed by euthanasia and liver collection. Plasma and bile underwent biochemical analyses, and histological, oxidative stress, and metabolic parameters were evaluated in the liver. Results: Both liraglutide treatment protocols attenuated hepatotoxicity that was induced by CCl4, decreasing plasma levels of hepatic enzymes, stimulating the hepatic antioxidant system, and decreasing centrilobular necrosis, hepatic glycogen, and lipid accumulation. CCl4 tended to reduce bile lipid excretion, but liraglutide did not influence this parameter. Conclusions: The present results demonstrated the hepatoprotective and therapeutic effects of liraglutide, which may be attributable to a decrease in liver oxidative stress and the preservation of metabolism. Liraglutide may have potential as a complementary therapy for acute liver injury

Dulaglutide as a demethylating agent to improve the outcome of breast cancer

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