Case Report: Giant cystic schwannoma of the middle mediastinum with cervical extension

Schwannomas (neurilemmomas) are benign tumors arising from the Schwann cells of the neural sheath. They are typically, well-encapsulated lesions which rarely adhere to the adjacent structures. In the chest, schwannomas are often seen within the posterior mediastinum and commonly originating along intercostal nerves. Several operative approaches have previously been described for the resection of these tumors, including thoracoscopic techniques and posterolateral thoracotomy. We report in this case a giant cystic mediastinal schwannoma of the left recurrent laryngeal nerve with cervical extension, unresectable by the usual described approaches, which was completely removed through a cervical approach.Keywords: mediastinal tumor; schwannoma; thoracotomy; cervicotom

Improved high gain observer design for a class of disturbed nonlinear systems

International audienceAbstract This paper provides a redesigned version of the Standard High Gain Observer (SHGO) to cope with the peaking phenomenon occurring during the transient periods as well as the sensitivity to high frequency measurement noise. The observer design is performed for a class of uniformly observable systems with noise free as well as noisy output measurements and the resulting observer is referred to as Non Peaking Filtered High Gain Observer (NPFHGO). The NPFHGO shares the same structure as its underlying SHGO and differs only by its corrective term which is still parameterized by a unique positive scalar up to an appropriate expression involving nested saturations. Of a fundamental interest, the power of the scalar parameter does not exceed one unlike in the case of the SHGO where this power grows from 1 to the system dimension. Moreover, it is shown that the equations of the NPFHGO become identical to those of the SHGO after a transient time horizon that can made arbitrarily small for sufficiently high values of the design parameter. A particular emphasis is put on the case of systems with noisy output measurements. It is shown how a multiple integrator of the corrupted outputs can be cascaded with the original system leading to an augmented system included in the class of systems for which the NPFHGO has been designed. The performance and main properties of the NPFHGO are highlighted and compared to those of its underlying SHGO through simulation results involving a single link robot arm system

Quantifying Intracellular Nanoparticle Distributions with Three-Dimensional Super-Resolution Microscopy

Super-resolution microscopy can transform our understanding of nanoparticle–cell interactions. Here, we established a super-resolution imaging technology to visualize nanoparticle distributions inside mammalian cells. The cells were exposed to metallic nanoparticles and then embedded within different swellable hydrogels to enable quantitative three-dimensional (3D) imaging approaching electron-microscopy-like resolution using a standard light microscope. By exploiting the nanoparticles’ light scattering properties, we demonstrated quantitative label-free imaging of intracellular nanoparticles with ultrastructural context. We confirmed the compatibility of two expansion microscopy protocols, protein retention and pan-expansion microscopy, with nanoparticle uptake studies. We validated relative differences between nanoparticle cellular accumulation for various surface modifications using mass spectrometry and determined the intracellular nanoparticle spatial distribution in 3D for entire single cells. This super-resolution imaging platform technology may be broadly used to understand the nanoparticle intracellular fate in fundamental and applied studies to potentially inform the engineering of safer and more effective nanomedicines

Quantifying Intracellular Nanoparticle Distributions with Three-Dimensional Super-Resolution Microscopy

Super-resolution microscopy can transform our understanding of nanoparticle–cell interactions. Here, we established a super-resolution imaging technology to visualize nanoparticle distributions inside mammalian cells. The cells were exposed to metallic nanoparticles and then embedded within different swellable hydrogels to enable quantitative three-dimensional (3D) imaging approaching electron-microscopy-like resolution using a standard light microscope. By exploiting the nanoparticles’ light scattering properties, we demonstrated quantitative label-free imaging of intracellular nanoparticles with ultrastructural context. We confirmed the compatibility of two expansion microscopy protocols, protein retention and pan-expansion microscopy, with nanoparticle uptake studies. We validated relative differences between nanoparticle cellular accumulation for various surface modifications using mass spectrometry and determined the intracellular nanoparticle spatial distribution in 3D for entire single cells. This super-resolution imaging platform technology may be broadly used to understand the nanoparticle intracellular fate in fundamental and applied studies to potentially inform the engineering of safer and more effective nanomedicines

Quantifying Intracellular Nanoparticle Distributions with Three-Dimensional Super-Resolution Microscopy

Super-resolution microscopy can transform our understanding of nanoparticle–cell interactions. Here, we established a super-resolution imaging technology to visualize nanoparticle distributions inside mammalian cells. The cells were exposed to metallic nanoparticles and then embedded within different swellable hydrogels to enable quantitative three-dimensional (3D) imaging approaching electron-microscopy-like resolution using a standard light microscope. By exploiting the nanoparticles’ light scattering properties, we demonstrated quantitative label-free imaging of intracellular nanoparticles with ultrastructural context. We confirmed the compatibility of two expansion microscopy protocols, protein retention and pan-expansion microscopy, with nanoparticle uptake studies. We validated relative differences between nanoparticle cellular accumulation for various surface modifications using mass spectrometry and determined the intracellular nanoparticle spatial distribution in 3D for entire single cells. This super-resolution imaging platform technology may be broadly used to understand the nanoparticle intracellular fate in fundamental and applied studies to potentially inform the engineering of safer and more effective nanomedicines