Nonvolatile read/write memory element - A concept

Memory, with limited number of programming cycles, is achieved by using verticle, fusible links in series with oxide breakthrough elements. Memory elements are fabricated with integrated circuit technology and are ideal for low power digital computer application

Non-perturbative Renormalization of Improved Staggered Bilinears

We compute Z-factors for general staggered bilinears on fine (a \approx 0.09 fm) MILC ensembles using both asqtad and HYP-smeared valence actions, comparing the results to the predictions of one-loop perturbation theory. This is an extension of previous work on the coarse (a \approx 0.12 fm) MILC ensembles. It provides a laboratory for studying NPR methodology in the staggered context, and is an important stepping stone for fully non-perturbative matching factors in ongoing computations of B_K and other weak matrix elements. We also implement non-exceptional RI/SMOM renormalization conditions using the asqtad action and present first results.Comment: 7 pages, 4 figures. Contribution to the 30th International Symposium on Lattice Field Theory, June 24-29, 2012, Cairns, Australi

Effects of an Acute Strength and Conditioning Bout on Dual Energy X-ray Absorptiometry Results

Dual Energy X-ray Absorptiometry (DXA) scans to assess body composition have become increasingly popular, especially in athletic populations. Acute factors, such as hydration status and food intake have been shown to alter DXA results (Tinsley, MSSE 2016). It is currently not known if prior strength and conditioning bouts may alter fat mass, lean mass, and bone density results. PURPOSE: To determine if a strength and conditioning (S&C) bout, similar to what athletes regularly engage in, will alter the fat mass, lean mass, and bone content results of a DXA scan. METHODS: Fourteen strength-trained subjects (10 men, 4 women, age 24 ± 2 yrs, height 176.7 ± 8.1 cm, weight 88.8 ± 14.7 kg) who were enrolled in an athletic strength and conditioning course volunteered to participate in this study. Each subject underwent two DXA scans on the same day. The first scan was performed prior to the S&C bout. The second scan was completed within 45 minutes after completion of the S&C bout. Participants were instructed to consume their normal, free living breakfast prior to scan one. A food and water log was distributed during the informed consent process and was maintained by the participants for 24 hours prior to all DXA scans. Nutritional information was analyzed via a commercial nutrition software for macronutrients, micronutrients, and hydration status. All DXA scans were performed and analyzed by the same trained technician. After the first scan, subjects were instructed to avoid all food intake until completion of the second scan. Subjects were encouraged to drink water ad libitum during the S&C bout from individually assigned 1-liter bottles; the volume consumed during the bout was measured by weight. RESULTS: No significant difference was found (correlated t-test a = 0.05) on any of the body composition measures between pre and post DXA body composition measurements after a S&C bout (changes pre to post: fat mass 46.5-46.0 kg, lean mass 64.8-64.9 kg, bone content 3.3-3.3 kg). CONCLUSION: Based on the results of the present study, S&C bouts do not need to be considered to ensure accuracy when performing DXA scans. The physiological changes that occur in response to a single S&C bout do not affect body composition analysis of DXA scans

APRIL:TACI axis is dispensable for the immune response to rabies vaccination.

There is significant need to develop a single-dose rabies vaccine to replace the current multi-dose rabies vaccine regimen and eliminate the requirement for rabies immune globulin in post-exposure settings. To accomplish this goal, rabies virus (RABV)-based vaccines must rapidly activate B cells to secrete antibodies which neutralize pathogenic RABV before it enters the CNS. Increased understanding of how B cells effectively respond to RABV-based vaccines may improve efforts to simplify post-exposure prophylaxis (PEP) regimens. Several studies have successfully employed the TNF family cytokine a proliferation-inducing ligand (APRIL) as a vaccine adjuvant. APRIL binds to the receptors TACI and B cell maturation antigen (BCMA)-expressed by B cells in various stages of maturation-with high affinity. We discovered that RABV-infected primary murine B cells upregulate APRIL ex vivo. Cytokines present at the time of antigen exposure affect the outcome of vaccination by influencing T and B cell activation and GC formation. Therefore, we hypothesized that the presence of APRIL at the time of RABV-based vaccine antigen exposure would support the generation of protective antibodies against RABV glycoprotein (G). In an effort to improve the response to RABV vaccination, we constructed and characterized a live recombinant RABV-based vaccine vector which expresses murine APRIL (rRABV-APRIL). Immunogenicity testing in mice demonstrated that expressing APRIL from the RABV genome does not impact the primary antibody response against RABV G compared to RABV alone. In order to evaluate the necessity of APRIL for the response to rabies vaccination, we compared the responses of APRIL-deficient and wild-type mice to immunization with rRABV. APRIL deficiency does not affect the primary antibody response to vaccination. Furthermore, APRIL expression by the vaccine did not improve the generation of long-lived antibody-secreting plasma cells (PCs) as serum antibody levels were equivalent in response to rRABV-APRIL and the vector eight weeks after immunization. Moreover, APRIL is dispensable for the long-lived antibody-secreting PC response to rRABV vaccination as anti-RABV G IgG levels were similar in APRIL-deficient and wild-type mice six months after vaccination. Mice lacking the APRIL receptor TACI demonstrated primary anti-RABV G antibody responses similar to wild-type mice following immunization with the vaccine vector indicating that this response is independent of TACI-mediated signals. Collectively, our findings demonstrate that APRIL and associated TACI signaling is dispensable for the immune response to RABV-based vaccination

B-meson decay constants: a more complete picture from full lattice QCD

We extend the picture of $B$-meson decay constants obtained in lattice QCD beyond those of the $B$, $B_s$ and $B_c$ to give the first full lattice QCD results for the $B^*$, $B^*_s$ and $B^*_c$. We use improved NonRelativistic QCD for the valence $b$ quark and the Highly Improved Staggered Quark (HISQ) action for the lighter quarks on gluon field configurations that include the effect of $u/d$, $s$ and $c$ quarks in the sea with $u/d$ quark masses going down to physical values. For the ratio of vector to pseudoscalar decay constants, we find $f_{B^*}/f_B$ = 0.941(26), $f_{B^*_s}/f_{B_s}$ = 0.953(23) (both $2\sigma$ less than 1.0) and $f_{B^*_c}/f_{B_c}$ = 0.988(27). Taking correlated uncertainties into account we see clear indications that the ratio increases as the mass of the lighter quark increases. We compare our results to those using the HISQ formalism for all quarks and find good agreement both on decay constant values when the heaviest quark is a $b$ and on the dependence on the mass of the heaviest quark in the region of the $b$. Finally, we give an overview plot of decay constants for gold-plated mesons, the most complete picture of these hadronic parameters to date.Comment: 20 pages, 9 figures. Minor updates to the discussion in several places and some additional reference

Electron Transfer Precedes ATP Hydrolysis during Nitrogenase Catalysis

The biological reduction of N2 to NH3 catalyzed by Mo-dependent nitrogenase requires at least eight rounds of a complex cycle of events associated with ATP-driven electron transfer (ET) from the Fe protein to the catalytic MoFe protein, with each ET coupled to the hydrolysis of two ATP molecules. Although steps within this cycle have been studied for decades, the nature of the coupling between ATP hydrolysis and ET, in particular the order of ET and ATP hydrolysis, has been elusive. Here, we have measured first-order rate constants for each key step in the reaction sequence, including direct measurement of the ATP hydrolysis rate constant: kATP = 70 s−1, 25 °C. Comparison of the rate constants establishes that the reaction sequence involves four sequential steps: (i) conformationally gated ET (kET = 140 s−1, 25 °C), (ii) ATP hydrolysis (kATP = 70 s−1, 25 °C), (iii) Phosphate release (kPi = 16 s−1, 25 °C), and (iv) Fe protein dissociation from the MoFe protein (kdiss = 6 s−1, 25 °C). These findings allow completion of the thermodynamic cycle undergone by the Fe protein, showing that the energy of ATP binding and protein–protein association drive ET, with subsequent ATP hydrolysis and Pi release causing dissociation of the complex between the Feox(ADP)2 protein and the reduced MoFe protein