6 research outputs found
Investigating Embryonic Expression Patterns and Evolution of AHI1 and CEP290 Genes, Implicated in Joubert Syndrome
Joubert syndrome and related diseases (JSRD) are developmental cerebello-oculo-renal syndromes with phenotypes
including cerebellar hypoplasia, retinal dystrophy and nephronophthisis (a cystic kidney disease). We have utilised the MRCWellcome
Trust Human Developmental Biology Resource (HDBR), to perform in-situ hybridisation studies on embryonic
tissues, revealing an early onset neuronal, retinal and renal expression pattern for AHI1. An almost identical pattern of
expression is seen with CEP290 in human embryonic and fetal tissue. A novel finding is that both AHI1 and CEP290
demonstrate strong expression within the developing choroid plexus, a ciliated structure important for central nervous
system development. To test if AHI1 and CEP290 may have co-evolved, we carried out a genomic survey of a large group of
organisms across eukaryotic evolution. We found that, in animals, ahi1 and cep290 are almost always found together;
however in other organisms either one may be found independent of the other. Finally, we tested in murine epithelial cells
if Ahi1 was required for recruitment of Cep290 to the centrosome. We found no obvious differences in Cep290 localisation
in the presence or absence of Ahi1, suggesting that, while Ahi1 and Cep290 may function together in the whole organism,
they are not interdependent for localisation within a single cell. Taken together these data support a role for AHI1 and
CEP290 in multiple organs throughout development and we suggest that this accounts for the wide phenotypic spectrum
of AHI1 and CEP290 mutations in man
<i>CEP290</i> is expressed in the developing cerebellum, spinal cord and eye.
<p>(A, G) Negative control hybridisation with <i>CEP290</i> sense RNA probe and (B–F) hybridisation with <i>CEP290</i> antisense RNA probe to transverse sections at CS22. <i>CEP290</i> transcripts are abundant in the developing telencephalon (B, C, E), especially the cortical neuroepithelium (C), and neural retina (D). <i>CEP290</i> expression is demonstrated within the epithelium of the choroid plexus (CP) (E, F) at CS22. Prominent expression is seen in metencephalon (including cerebellum), myelencephalon and mesencephalon at CS19 (H, I, J). Strong expression is detected at the rhombic lip in the developing cerebellum (H, J), the mesencephalic (I) and cerebellar neuroepithelium (J). Prominent staining is demonstrated within the alar plate of spinal cord at CS16 (K) and CS22 (L). Ap, alar plate; Bp, basal plate; CP: choroid plexus; CPE, choroid plexus epithelium; Mes, mesencephalon (midbrain); Met, metencephalon; Mye, myelencephalon; NEP, neuroepithelium; Cor.NEP: cortical neuroepithelium; Cere.NEP: cerebellar neuroepithelium; Mes.NEP: mesencephalic (midbrain) neuroepithelium; NR, neural retina; RPE, retinal pigment epithelium, Sc, spinal cord; SG: spinal ganglion; SyG: sympathetic ganglia; T, telencephalon, Ton, tongue; LRhoL: lower rhombic lip; URhoL: upper rhombic lip; Scale bar: A, B, G, H = 2 mm, C, D, F, K = 500 µm; E = 1 mm; L = 500 µm; I, J = 250 µm.</p
Cep290 does not require Ahi1 for localisation to centrosomes.
<p>Immunofluorescence of IMCD3 monolayers co-transfected with siGLO and either negative-control siRNA (A and B), or siRNA against <i>Ahi1</i> (A and C). (A) Graph to show centrosome position (gamma tubulin immunofluorescence, graph expressed as mean +/− s.e.m.) from apical to basal in control and <i>Ahi1</i>-silenced cells. Apical refers to the upper 1.5–2 µm of the cell (the distance between the nuclear envelope and the plasma membrane in these cells; this is unchanged following silencing of <i>Ahi1</i>, data not shown). Arrows denote the apical and mid-cell positions used in B and C. (B and C) Confocal maximum intensity projections of control (B) and <i>Ahi1</i>-silenced (C) cells showing Cep290 (red), gamma tubulin (green) and DNA (blue). Transfected cells were identified using siGLO (yellow). In each case, the top panel represents an apically-oriented centrosome (position 1 on the graph, A) and the bottom panel represents a centrosome from the mid-cell region (position 2 on the graph). Scale bar: 5 µm.</p
<i>AHI1</i> expression during nephrogenesis.
<p>(A, C, E) <i>AHI1</i> sense RNA probe and (B, D, F) <i>AHI1</i> antisense RNA probe hybridised to sections from (A, B) CS16; (C, D) CS22 and (E, F) 9 PCW. <i>AHI1</i> transcripts are abundant in the mesonephros (Meso) and metanephros (Meta) of the developing kidney as well as the spinal cord (Sc), liver and embryonic gonad. (G, H). A series of sections at different stages of mesonephric and metanephric development. <i>AHI1</i> expression is seen in (G) mesonephric excretory unit - a mesonephric tubule, glomerulus and duct at CS14, CS16 and CS22. By CS23 expression is visible in degenerating glomeruli (DG). By 9 PCW expression is detectable in the mesonephric tubule, ducts and paramesonephric duct. <i>AHI1</i> expression is seen in (H) early permanent metanephric kidney with intense staining in the metanephric cap and ureteric bud (CS14 and CS16). By CS22 and later developing glomeruli, tubules and collecting ducts are seen to strongly express <i>AHI1</i> (CS22, CS23 and 9 PCW). In 9 PCW human fetal sections, <i>AHI1</i> transcripts are abundant in the developing nephrons and collecting ducts of renal cortex and there is weak expression in medulla/renal pelvis. CD, collecting duct; DG, degenerating glomerulus; Gl, glomerulus; H, hindgut; LL, lower limb; Mc, metanephric cap; Md, mesonephric duct; Med, renal medulla; Meso, mesonephros; Meta, metanephros; Mt, mesonephric tubule; P, renal pelvis; Pmeso, paramesonephric duct; Sc, spinal cord; St, stomach; UB, ureteric bud; UL, upper limb. Scale bars: A–F = 2 mm; G: CS16 = 500 µm; CS14, CS23 & 9WPC = 250 µm; CS22 = 125 µm; H: CS22 & 9WPC = 500 µm; CS14, CS16 & CS23 = 250 µm.</p
<i>CEP290</i> is expressed in the developing kidney.
<p>Hybridisation with <i>CEP290</i> antisense RNA probe (A–F) to transverse sections. (A) CS12 and (B) CS14; (C) CS15; (D) CS16. <i>CEP290</i> transcripts are abundant in the mesonephros (Meso) and metanephros (Meta) of the developing kidney as well as the spinal cord (Sc). (E, F) A series of sections through mesonephric and metanephric development, respectively, from CS14 to 9 PCW. Hybridisations were also carried out with <i>CEP290</i> sense probe as a negative control and no signals were detected (data not shown). CD, collecting duct; Gl, glomerulus; H, hindgut; Meso, mesonephros; DMeso, degenerating mesonephros; Meta, metanephros; Sc, spinal cord; St, stomach; UL, upper limb; LL, lower limb. P, renal pelvis. Scale bar: A–D = 1 mm; E: CS12–CS16 = 500 µm; CS23 = 125 µm F: CS 14 = 250 µm; CS17 = 125 µm; CS20 = 250 µm; CS22 and 9 PCW = 125 µm.</p