Device-independent, real-time identification of bacterial pathogens with a metal oxide-based olfactory sensor

A novel olfactory method for bacterial species identification using an electronic nose device called the MonoNose was developed. Differential speciation of micro-organisms present in primary cultures of clinical samples could be performed by real-time identification of volatile organic compounds (VOCs) produced during microbial replication. Kinetic measurements show that the dynamic changes in headspace gas composition are orders of magnitude larger than the static differences at the end of fermentation. Eleven different, clinically relevant bacterial species were included in this study. For each of the species, two to eight different strains were used to take intra-species biodiversity into account. A total of 52 different strains were measured in an incubator at 37°C. The results show that the diagnostic specificities varied from 100% for Clostridium difficile to 67% for Enterobacter cloacae with an overall average of 87%. Pathogen identification with a MonoNose can be achieved within 6–8 h of inoculation of the culture broths. The diagnostic specificity can be improved by broth modification to improve the VOC production of the pathogens involved

Assessment of genetic parameters of degradability in maize grains due to indentation scores

Maize (Zea mays L.) cultivars with dent grains present higher starch and dry matter ruminal degradability than those with flint grains. This study aimed to assess genetic parameters of degradability in maize grains of different textures and to verify whether there is an association between grain indentation score and ruminal dry matter (RDM) degradability. The evaluated progenies were obtained from the cross between the varieties Cristal (flint) and Cunha (dent), previously selected for grain hardness. Progenies were evaluated for an indentation score using a visual grading scale ranging from 1 (flint) to 5 (dent). To assess the RDM degradability, 5 g were cut into halves, weighed (five grams), stored in bags made of 100% "failet" cloth and incubated for 24 h in the rumen of three ruminally cannulated cows. There was genetic variability for RDM degradability and heritability was 46%, indicating the possibility of success using a selection. There was no association between indentation score and RDM degradability percentage; thus, visual evaluation not always reflects grain hardness per se since it indicates the pericarp aspect but not the endosperm constitution.Cultivares de milho (Zea mays L.) com grãos do tipo dentado possuem maior porcentagem de amido e degradabilidade ruminal da matéria seca do que os com grãos do tipo duro. Estimaram-se parâmetros genéticos da degradabilidade de grãos de milho com diferentes texturas e verificou-se se ocorre associação entre o escore de identação dos grãos visualmente avaliados e a degradabilidade in situ da matéria seca (DISMS). Foram avaliadas progênies oriundas do cruzamento entre a variedade Cristal, de grãos duros, e a variedade Cunha de grãos dentados, previamente selecionadas para a dureza dos grãos. As progênies foram avaliadas com relação ao escore de identação por meio de uma escala visual de notas variando de 1 (duro) a 5 (mole). Para a avaliação da DISMS os grãos foram cortados ao meio, pesados 5 g colocados em saquinhos de tecido "failet" e incubados no rúmen, por 24 h, em três vacas portadoras de cânula ruminal. Há variabilidade genética para a DISMS, com herdabilidade de 46%, indicando ser possível o sucesso com a seleção. Não há associação entre o escore de identação e a porcentagem de DISMS. Assim, a avaliação visual nem sempre reflete a dureza per se dos grãos, pois o que se vê é o aspecto do pericarpo e não a constituição do endosperma