University textbook on oral mucosal diseases

This textbook is primarily intended for students of dental medicine, it can also be useful for the students of general medicine whom it can help in clinical and field practice. The term “diseases of the oral mucosa” describes a diverse group of diseases of various aetiologies and seriousness and this issue is closely related to dermatovenerological, otorhinolaryngological, ophthalmological, rheumatological and internal diseases. The dentist may often be the first physician noticing and possibly diagnosing a general disease based on symptoms of the oral cavity. The new extended edition reflects the improving knowledge of the etiopathological processes of oral mucosal diseases on the molecular level and the ever increasing range of diagnostic laboratory tests and/or therapeutic options

Platelet-rich plasma, platelet-rich fibrin, and enamel matrix derivative for oral mucosal wound healing

Different approaches to enhance healing of hard or soft tissues include the use of cytokines and growth factors to modify cellular behaviour. Numerous growth factors are found in autologous blood concentrates – platelet rich plasma (PRP) and platelet rich fibrin (PRF). Enamel matrix derivative (EMD) may improve tissue healing via amelogenins. Bilayered collagen matrix (CM) is used for soft tissue augmentation. The aim of the present study was to assess potential benefits of PRP, PRF and EMD in combination with bilayered collagen matrix or CM alone in treatment of oral mucosal defects in rabbits. Twenty-seven New Zealand white rabbits were included in this randomized controlled trial. Artificial oral mucosal defects were treated with one of these five approaches: PRP+CM, PRF+CM, EMD+CM, CM alone, or left untreated as a negative control (CO). The animals were euthanized 1 day, 7 days, or 28 days after surgery and necropsies were harvested. Histological and molecular biological analyses were performed. All defects were healed by day 28. No differences between PRP+CM, PRF+CM, CM alone and CO groups were recorded at any time point. Slower angiogenesis and a higher presence of inflammatory infiltrate were observed in the EMD+CM group 28 days after surgery. Molecular biological analyses did not reveal any statistically significant changes. In conclusion, no improvement in mucosal healing of wounds covered with a collagen membrane and PRP, PRF, or EMD was observed, compared with CM alone or untreated controls.Different approaches to enhance healing of hard or soft tissues include the use of cytokines and growth factors to modify cellular behaviour. Numerous growth factors are found in autologous blood concentrates – platelet rich plasma (PRP) and platelet rich fibrin (PRF). Enamel matrix derivative (EMD) may improve tissue healing via amelogenins. Bilayered collagen matrix (CM) is used for soft tissue augmentation. The aim of the present study was to assess potential benefits of PRP, PRF and EMD in combination with bilayered collagen matrix or CM alone in treatment of oral mucosal defects in rabbits. Twenty-seven New Zealand white rabbits were included in this randomized controlled trial. Artificial oral mucosal defects were treated with one of these five approaches: PRP+CM, PRF+CM, EMD+CM, CM alone, or left untreated as a negative control (CO). The animals were euthanized 1 day, 7 days, or 28 days after surgery and necropsies were harvested. Histological and molecular biological analyses were performed. All defects were healed by day 28. No differences between PRP+CM, PRF+CM, CM alone and CO groups were recorded at any time point. Slower angiogenesis and a higher presence of inflammatory infiltrate were observed in the EMD+CM group 28 days after surgery. Molecular biological analyses did not reveal any statistically significant changes. In conclusion, no improvement in mucosal healing of wounds covered with a collagen membrane and PRP, PRF, or EMD was observed, compared with CM alone or untreated controls

Repetitorium onemocnění sliznice ústní dutiny

Title in English: University textbook on oral mucosal diseases. The textbook is primarily intended for students of dental medicine, it can also be useful for the students of general medicine whom it can help in clinical and field practice. The term “diseases of the oral mucosa” describes a diverse group of diseases of various aetiologies and seriousness and this issue is closely related to dermatovenerological, otorhinolaryngological, ophthalmological, rheumatological and internal diseases. The dentist may often be the first physician noticing and possibly diagnosing a general disease based on symptoms of the oral cavity. The new extended edition reflects the improving knowledge of the etiopathological processes of oral mucosal diseases on the molecular level and the ever increasing range of diagnostic laboratory tests and/or therapeutic options

Granulation tissue enriched by aspirin and omega-3 fatty acids in healing experimental periodontal lesion

Aims. Granulation tissue (GT) and specialized proresolving mediators such as lipoxins and resolvins are key elements in the successful resolution of periodontitis. Aspirin triggered lipoxins and resolvins are even more powerful than their natural analogues. Their biosynthesis can be accelerated by omega3 fatty acids. The aim of this study was to evaluate the use of GT enriched by aspirin and omega‑3 fatty acids during the surgical treatment of periodontitis in an experimental animal model (rabbit). Methods. In each of 24 rabbits, two experimental periodontal defects were created. In total, 47 defects were treated with open flap debridement and one of three procedures: (1) GT extracted and soaked with aspirin and omega‑3 fatty acids (ASA+OMEGA3 group); (2) GT soaked with saline (PLACEBO group); or (3) GT left untreated (CONTROL group). Then, the GT was replaced in situ. Primary evaluated criteria were the probing pocket depth (PPD) and the clinical at‑ tachment level (CAL). Necropsies were harvested 2, 6, and 12 weeks after surgery. The samples were used for histological and molecular biological assessment. Results. A trend of greater PPD and CAL in the ASA+OMEGA3 group was observed at 6 weeks. However, there was no significant difference between them. During the observation period, tissue levels of FGF‑7, IL1β and TIMP1 showed a statistically significant decrease (P<0.05). For the other variables, the ASA+OMEGA3 group was comparable with the PLACEBO and CONTROL groups. Conclusion. This experiment did not demonstrate the superiority of the proposed approach. However, the enriched granulation tissue did not impair healing outcomes

Onemocnění sliznice ústní dutiny – repetitorium a atlas

Multimediální publikace Onemocnění sliznice ústní dutiny – repetitorium a atlas zahrnuje učební text a k němu příslušející obrazovou databázi patologických stavů na sliznicích dutiny ústní. Úvodní kapitoly zahrnují obecné poznatky o ústních sliznicích. Dále následují kapitoly týkající se jednotlivých onemocnění včetně diferenciálně diagnostických poznámek. Publikace je určena studentům programu Zubní lékařství a Všeobecné lékařství.This electronic publication includes a textbook and an associated image database of pathological conditions on the mucous membranes of the oral cavity. The introductory chapters include general knowledge about the oral mucosa. The following are chapters on individual diseases, including differential diagnostic notes

The Use of Confocal Laser Endomicroscopy in Diagnosing Barrett’s Esophagus and Esophageal Adenocarcinoma

Confocal laser endomicroscopy (CLE) is a diagnostic technique that enables real-time microscopic imaging during microscopic examination and evaluation of epithelial structures with 1000-fold magnification. CLE can be used in the diagnosis of various pathologies, in pneumology, and in urology, and it is very widely utilized in gastroenterology, most importantly in the diagnosis of Barrett’s esophagus (BE), esophageal adenocarcinoma (EAC), biliary strictures, and cystic pancreatic lesions. A literature search was made in MEDLINE/PubMed and Google Scholar databases while focusing on diagnostics using CLE of BE and EAC. We then examined randomized and observational studies, systematic reviews, and meta-analyses relating to the utilization of CLE in BE and EAC diagnostics. Here, we discuss whether CLE can be a suitable diagnostic method for surveillance of BE. Even though many studies have proven that CLE increases diagnostic accuracy in detecting neoplastic transformation of BE, CLE is still not used as a standard diagnostic tool in BE surveillance due to a deficiency of scientific evidence. More studies and data are needed if CLE is to find a place as a new technique in BE surveillance