Effects of different feeds and stocking densities on growth and survival rates of mud crab (<em>Scylla paramamosain</em>) at the stage from megalopa to crablet-1

Mud crabs (Scylla genus) are luxury foods in high demand internationally. The efficient techniques for mud crab hatcheries are vital for providing breeds for their aquaculture, which is rapidly growing in many countries. This study aims to investigate the effects of different feeds and stocking densities on mud crabs' growth and survival rates (Scylla paramamosain) in the stage from megalopa to crablet-1 stage. Two separate experiments were conducted indoors in the 60-liter round plastic tanks (containing 50 liters of water at a 28‰ salinity). Experiment 1 investigated four feeds: frozen Artemia biomass, pureed shrimp meat, Lansy pellet feed (48% protein), and NRD pellet feed (55% protein). Megalopae (mean weight of 5.8 mg) were stocked at a density of 10/L. In experiment 2, the megalopae (mean weight of 5.4 mg) were stocked at densities of 20, 30, and 40/L and were fed the Lansy pellet feed, which was the best one selected from experiment 1. High survival rates were obtained at all four feeds (82.2–87.5%) and three stocking densities (88.4–90.1%). The growth performances in Lansy feed and frozen Artemia biomass were better than those in pureed shrimp meat and NRD pellet feed, which was seen through higher indicators of daily weight gain (DWG) and specific growth rate in weight (SGRw) (p p > 0.05). The investigated feeds and stocking densities suit the nursing mud crab (S. paramamosain) megalopa. In contrast, the Lansy pellet feeds had a stocking density of 20/L, resulting in the highest nursing efficiency

Unexpected cases in field diagnosis of African swine fever virus in Vietnam: The needs consideration when performing molecular diagnostic tests

Background: The first confirmed case of African swine fever (ASF) in Vietnam was reported officially in February 2019. To date, ASF virus (ASFV) have been detected in 63/63 provinces in Vietnam. Currently, real-time polymerase chain reaction (PCR) is considered to be a powerful tool for viral detection in field samples, including ASFV. However, some recent reports have suggested that mismatches in primer and probe binding regions may directly affect real-time PCR qualification, leading a false-negative result.Aim: This study aims to further examine a conflicting result obtained from two OIE recommended methods, conventional PCR and real-time PCR, for ASFV detection.Methods: Two ASF suspected pigs from different provinces in the north of Vietnam were selected for this study based on clinical signs and postmortem lesions. The different results obtained by OIE-recommended conventional PCR and real-time PCR were further analyzed by the Sanger sequencing method and virus isolation in combination with hemadsorption (HAD) test using porcine alveolar macrophages cells.Results: The results showed that when the primer sequence matched perfectly with the sequences of field isolates, a mutation in probe binding region was found, indicating that a single mismatch in the probe binding site may cause a false-negative result by real-time PCR in detecting ASFV in clinical samples in Vietnam. An agreement between conventional PCR, using PPA1/PPA2 primers and two golden standard methods, virus isolation in combination with HAD assay, and sequencing method was observed in this study.Conclusion: A single mismatch in the probe binding site caused a failse-negative result by realtime PCR method in field diagnosis of ASFV. The needs consideration when selecting the appropriate molecular diagnostic methods is based on the current databases of ASFV sequences,&nbsp; particularly for epidemiological surveillance of ASF. Keywords: African swine fever, PCR, Pigs, Real-time PCR, Vietna

Gauge Bosons in the 3-3-1 Model with Three Neutrino Singlets

We show that the mass matrix of electrically neutral gauge bosons in the recent proposed  model based on $\mathrm{SU}(3)_C\otimes \mathrm{SU}(3)_L\otimes \mathrm{U}(1)_X$ group with three neutrino singlets [9] has two exact eigenvalues and corresponding eigenvectors. Hence the neutral non-Hermitian gauge boson $X^0_\mu$ is properly determined.With extra vacuum expectation values of the Higgs fields, there are mixings among charged gauge bosons $W^\pm$ and $Y^\pm$ as well as amongneutral gauge bosons $Z, Z'$ and $X^0$. From the $W$ boson decay width, we get lower bound on  scale of the model about few TeVs

ARtVista: Gateway To Empower Anyone Into Artist

Drawing is an art that enables people to express their imagination and emotions. However, individuals usually face challenges in drawing, especially when translating conceptual ideas into visually coherent representations and bridging the gap between mental visualization and practical execution. In response, we propose ARtVista - a novel system integrating AR and generative AI technologies. ARtVista not only recommends reference images aligned with users' abstract ideas and generates sketches for users to draw but also goes beyond, crafting vibrant paintings in various painting styles. ARtVista also offers users an alternative approach to create striking paintings by simulating the paint-by-number concept on reference images, empowering users to create visually stunning artwork devoid of the necessity for advanced drawing skills. We perform a pilot study and reveal positive feedback on its usability, emphasizing its effectiveness in visualizing user ideas and aiding the painting process to achieve stunning pictures without requiring advanced drawing skills. The source code will be available at https://github.com/htrvu/ARtVista.Comment: CHI 202

Vehicle Type Classification with Small Dataset and Transfer Learning Techniques

This study delves into the application of deep learning training techniques using a restricted dataset, encompassing around 400 vehicle images sourced from Kaggle. Faced with the challenges of limited data, the impracticality of training models from scratch becomes apparent, advocating instead for the utilization of pre-trained models with pre-trained weights. The investigation considers three prominent models—EfficientNetB0, ResNetB0, and MobileNetV2—with EfficientNetB0 emerging as the most proficient choice. Employing the gradually unfreeze layer technique over a specified number of epochs, EfficientNetB0 exhibits remarkable accuracy, reaching 99.5% on the training dataset and 97% on the validation dataset. In contrast, training models from scratch results in notably lower accuracy. In this context, knowledge distillation proves pivotal, overcoming this limitation and significantly improving accuracy from 29.5% in training and 20.5% in validation to 54% and 45%, respectively. This study uniquely contributes by exploring transfer learning with gradually unfreeze layers and elucidates the potential of knowledge distillation. It highlights their effectiveness in robustly enhancing model performance under data scarcity, thus addressing challenges associated with training deep learning models on limited datasets. The findings underscore the practical significance of these techniques in achieving superior results when confronted with data constraints in real-world scenario

Assessment of the Effectiveness of Ich Tam Khang as a Supportive Therapy for Chronic Heart Failure

Background: Heart failure is a chronic disease needing lifelong management. Despite the advances that have been made in the treatment of the disease, both the longevity and quality of life for those with chronic heart failure remain impaired. A more effective therapeutic approach with less negative side effects is still needed. In this study, we evaluate Ich Tam Khang (ITK), the poly-ingredient herbal and nutritional preparation with multiple physiological actions, as a supportive therapy for patients with chronic heart failure.Aims of Study: To evaluate the effectiveness and safety of Ich Tam Khang as an adjunctive treatment of chronic heart failure.Methods: A total of 60 patients with chronic congestive heart failure were enrolled in this open label, cross-sectional and prospective study. All patients were treated with a conventional regimen (digoxin, diuretics, angiotensin-converting-enzyme (ACE) inhibitors or angiotensin II receptor blockers (ARBs), beta blockers) for at least 4 weeks before being divided into two equal groups. In the treated patients with ITK, patients received conventional therapy plus 4 tablets ITK per day added in two divided doses. In the control patients, all patients kept the same conventional regimen without ITK. All patients were followed up for 3 months for clinical and para-clinical outcomes.Result: The symptoms of heart failure (dyspnea, palpitation, peripheral edema, neck vein distention, heptojugular reflex) decreased. Heart rate and blood pressure stabilized during treatment in the treated patients with ITK. Additionally, total cholesterol and HDL-cholesterol normalized in the patients treated with ITK. Most of echocardiography parameters in the ITK treated patients were superior to the control patients. ITK is safe and it has no side effects.Conclusion: ITK as a combination of herbal and nutritional preparation is effective in reducing heart failure symptoms, improving patient's quality of life for the patients with decompensated heart failure and reducing total cholesterol and LDL-C

Beyond First/Last Mile Active Transportation - BikeShare@UH

Bike sharing is a new green transportation solution that has been developed and adopted at various cities around the world. In this paper, we present the process and results of the design and prototypes that a group of undergraduate students developed for a BikeShare@UH program during Summer 2017. After presenting the detailed results of four project teams focusing on customer discovery, bike share station (BSS) location identification, cloud-based mobile computing platform for user engagement and bike share program operation and management, smart lock, and alternative energy source based on PV panel. With the phase one implementation at the University planned in Spring 2018, we anticipate gathering real time data and feedback to improve the system.Cockrell School of Engineerin

Polyculture of red seaweed (<i>Gracilaria tenuistipitata</i>) with different stocking densities of whiteleg shrimp (<i>Litopenaeus vannamei</i>): Effects on water quality and shrimp performance

This study was conducted to determine the impact of coculturing red seaweed (Gracilaria tenustipitata) with different densities of whiteleg shrimp (Litopenaeus vannamei) on water quality and shrimp performance. The experiment was carried out in indoor tank systems in triplicate. Five shrimp densities (100, 200, 300, 400, and 500 shrimp/m3) were integrated with red seaweed (2 kg/m3) for 60 days. The results demonstrated that polyculture of red seaweed with shrimp maintained appropriate levels of TAN and NO2- in rearing tanks at high shrimp densities ranging from 100 to 300 shrimp/m3. Shrimp growth rates tended to decline as density increased, but there were no statistical differences (p>0.05) between treatments at stocking densities ranging from 100 to 300 shrimp/m3. Shrimp survival declined dramatically as stocking densities increased from 400 shrimp/m3 upward. The lowest and maximum shrimp yields were achieved at densities of 100 and 300 shrimp/m3, respectively. Furthermore, the sensory quality of cooked shrimp meat was highly rated by the panelists. These findings demonstrated that polyculture of red seaweed with whiteleg shrimp can be applied at stocking densities up to 300 shrimp/m3 while maintaining appropriate water quality parameters and improved production efficiency in the culture unit

Brza serotipizacija virusa afričke kuge svinja zasnovana na analizi kratkih fragmenata EP402R gena koji kodira CD2-like protein

The first confirmed case of African swine fever (ASF) in Vietnam was officially reported in February 2019. To date, the ASF virus (ASFV) has been detected in all 63 cities/provinces in Vietnam. In order to get a better understanding of the potential role of the EP402R gene in a grouping of ASFV serotypes, thirty ASFV sequences of EP402R genes (accession numbers: MN711757-86) from North Central Coast of Vietnam and 68 well-known references of serotype groups from previous studies were further analyzed. Interestingly, we found that a short fragment of 90 nucleotides was very typical for 8 serological groups of ASFVs. A primer set was designed to amplicon the short fragment of 90 nucleotides using the Primer3 program to establish a simplified method for the serotyping of ASFV. Our results indicated that phylogenetic analysis of the short fragment (90 nucleotides) of the EP402R gene is a very specific and useful method for ASFV serotyping when compared to the previous method using a long fragment (816 nucleotides) of this gene and well-known serotype references based on haemadsorption inhibition (HAI) assay.Prvi potvrđen slučaj afričke kuge svinja (ASF) u Vijetnamu bio je zvanično prijavljen u februaru 2019. godine. Do danas, ASF virus (ASFV) je detektovan u 63 provincije Vijetnama. Radi boljeg razumevanja potencijalne uloge EP402R gena u grupisanju ASFV serotipova, analizirano je trideset ASFV sekvenci EP402R gena (pristupni brojevi: MN711757-86) poreklom iz Centralne severne obale Vijetnama i 68 referentnih uzoraka serotip grupa koji su dobro poznati iz prethodnih studija. Interesantan je bio nalaz da je kratak fragment od 90 nukleotida bio tipičan za 8 seroloških grupa ASF virusa. Dizajniran je set prajmera sa ciljem amplifi kacije kratkih fragmenata (90 nukleotida), korišćenjem Primer3 programa, a za uspostavljanje jednostavne metode serotipiziranja ASFV. Rezultati ukazuju da je fi logenetska analiza kratkih fragmenata (90 nukleotida) EP402R gena je veoma specifična i predstavlja korisnu metodu serotipizacije ASFV u poređenju sa prethodno opisanim metodama koje su koristile duge fragmente (816 nukleotida) ovog gena kao i dobro poznate referentne serotipove dobijene metodom inhibicije hemadsorbcije

Network Coding with Multimedia Transmission and Cognitive Networking: An Implementation based on Software-Defined Radio

Network coding (NC) is considered a breakthrough to improve throughput, robustness, and security of wireless networks. Although the theoretical aspects of NC have been extensively investigated, there have been only few experiments with pure NC schematics. This paper presents an implementation of NC under a two-way relay model and extends it to two non-straightforward scenarios: (i) multimedia transmission with layered coding and multiple-description coding, and (ii) cognitive radio with Vandermonde frequency division multiplexing (VFDM). The implementation is in real time and based on software-defined radio (SDR). The experimental results show that, by combining NC and source coding, we can control the quality of the received multimedia content in an on-demand manner. Whereas in the VFDM-based cognitive radio, the quality of the received content in the primary receiver is low (due to imperfect channel estimation) yet retrievable. Our implementation results serve as a proof for the practicability of network coding in relevant applications