The Vehicle, Fall 2009

Table of Contents Poetry AliveRashelle McNairpage 3 Train of ThoughtsJeanette Saribekianpage 4 Biding the TideMarlee Lutzpage 5 Rotten HarvestJessyca Revillapage 15 Nostalgia ODJustine Fittonpage 16 Beyond WordsAshley Wrightpage 26 Don\u27tMelinda Knightpage 27 Happy HourStephen Garciapage 35 UntitledDaniel Paquinpage 37 Vibrant SensationsAshton Tembypage 38 Scarecrow Sally on a Saturday NightDaniel Davispage 45 The FarmAshley Wrightpage 49 Anything ButJustine Fittonpage 51 CrashDanielle Shirtinopage 53 Weathering SatisfactionRashelle McNairpage 54 SeminarDaniel Davispage 71 Nature\u27s Mood SwingsJeanette Saribekianpage 72 The PerformanceMelinda Knightpage 68 AmaterasuMarlee Lutzpage 82 Prose AirLauren Davidsonpage 6 The Twang of OrangesJ.T. Dawsonpage 18 ListenStephani Pescitellipage 29 The Rise and Fall of NickNickolas Alexanderpage 30 LossSimyona Deanovapage 39 Like DiamondsMark Rheaumepage 42 Moral FixationBryan Rolfsenpage 47 Reflections in College AlgebraNicole Reichertpage 52 LeashDaniel Paquinpage 56 I Lost My KeysJustine Fittonpage 75 A Third Grade EssayMark Rheaumepage 69 Be Careful, They BiteDaniel Davispage 84 Art Limb BurgAlycia Rockeycover AvesSamantha Flowerspage 14 Life-LuminescenceStephani Pescitellipage 25 MonopolyMegan Mathypage 28 Carousel NostalgiaAlycia Rockeypage 36 ShoesSarah Olsonpage 41 Waimea BayJarrod Taylorpage 50 Peacock Plumage Alycia Rockeypage 55 Building a HouseStephani Pescitellipage 70 ShellMegan Mathypage 74 From the VacationSamantha Flowerspage 73 Chicago CanopyAlycia Rockeypage 83 Features Editor\u27s NoteLindsey Durbinpage 1 LazarusDr. David Radavichpage 2 James K. Johnson Creative Writing Awardpage 88 Winning Entries (Poetry)Matthew J. Schumakepage 89 Winning Entry (Nonfiction)Jennifer O\u27Neilpage 92 Interview, 2009 Chapbook WinnerDaniel Davispage 95 Contributorspage 99https://thekeep.eiu.edu/vehicle/1090/thumbnail.jp

The Vehicle, Fall 2009

Table of Contents Poetry AliveRashelle McNairpage 3 Train of ThoughtsJeanette Saribekianpage 4 Biding the TideMarlee Lutzpage 5 Rotten HarvestJessyca Revillapage 15 Nostalgia ODJustine Fittonpage 16 Beyond WordsAshley Wrightpage 26 Don\u27tMelinda Knightpage 27 Happy HourStephen Garciapage 35 UntitledDaniel Paquinpage 37 Vibrant SensationsAshton Tembypage 38 Scarecrow Sally on a Saturday NightDaniel Davispage 45 The FarmAshley Wrightpage 49 Anything ButJustine Fittonpage 51 CrashDanielle Shirtinopage 53 Weathering SatisfactionRashelle McNairpage 54 SeminarDaniel Davispage 71 Nature\u27s Mood SwingsJeanette Saribekianpage 72 The PerformanceMelinda Knightpage 68 AmaterasuMarlee Lutzpage 82 Prose AirLauren Davidsonpage 6 The Twang of OrangesJ.T. Dawsonpage 18 ListenStephani Pescitellipage 29 The Rise and Fall of NickNickolas Alexanderpage 30 LossSimyona Deanovapage 39 Like DiamondsMark Rheaumepage 42 Moral FixationBryan Rolfsenpage 47 Reflections in College AlgebraNicole Reichertpage 52 LeashDaniel Paquinpage 56 I Lost My KeysJustine Fittonpage 75 A Third Grade EssayMark Rheaumepage 69 Be Careful, They BiteDaniel Davispage 84 Art Limb BurgAlycia Rockeycover AvesSamantha Flowerspage 14 Life-LuminescenceStephani Pescitellipage 25 MonopolyMegan Mathypage 28 Carousel NostalgiaAlycia Rockeypage 36 ShoesSarah Olsonpage 41 Waimea BayJarrod Taylorpage 50 Peacock Plumage Alycia Rockeypage 55 Building a HouseStephani Pescitellipage 70 ShellMegan Mathypage 74 From the VacationSamantha Flowerspage 73 Chicago CanopyAlycia Rockeypage 83 Features Editor\u27s NoteLindsey Durbinpage 1 LazarusDr. David Radavichpage 2 James K. Johnson Creative Writing Awardpage 88 Winning Entries (Poetry)Matthew J. Schumakepage 89 Winning Entry (Nonfiction)Jennifer O\u27Neilpage 92 Interview, 2009 Chapbook WinnerDaniel Davispage 95 Contributorspage 99https://thekeep.eiu.edu/vehicle/1090/thumbnail.jp

MeCP2 binds to nucleosome free (linker DNA) regions and to H3K9/H3K27 methylated nucleosomes in the brain

Methyl-CpG-binding protein 2 (MeCP2) is a chromatin-binding protein that mediates transcriptional regulation, and is highly abundant in brain. The nature of its binding to reconstituted templates has been well characterized in vitro. However, its interactions with native chromatin are less understood. Here we show that MeCP2 displays a distinct distribution within fractionated chromatin from various tissues and cell types. Artificially induced global changes in DNA methylation by 3-aminobenzamide or 5-aza-2′-deoxycytidine, do not significantly affect the distribution or amount of MeCP2 in HeLa S3 or 3T3 cells. Most MeCP2 in brain is chromatin-bound and localized within highly nuclease-accessible regions. We also show that, while in most tissues and cell lines, MeCP2 forms stable complexes with nucleosome, in brain, a fraction of it is loosely bound to chromatin, likely to nucleosome-depleted regions. Finally, we provide evidence for novel associations of MeCP2 with mononucleosomes containing histone H2A.X, H3K9me2 and H3K27me3 in different chromatin fractions from brain cortex and in vitro. We postulate that the functional compartmentalization and tissue-specific distribution of MeCP2 within different chromatin types may be directed by its association with nucleosomes containing specific histone variants, and post-translational modifications