Stomatal abundance and distribution in Prosopis strombulifera plants growing under different Iso-Osmotic salt

Changes in several environmental parameters are thought to affect stomatal development. Under salt stress, plants can regulate their transpiration flux through a better control of the stomatal opening (as a short-term response) and through modifications of leaf anatomy (as a long-term response). We investigate how leaf micromorphology (stomatal abundance and distribution) of the halophyte Prosopis strombulifera (a spiny shrub particularly abundant in high-salinity areas of central Argentina) responds to different water status when plants are subjected to different salt treatments (NaCl, Na2SO4 and their iso-osmotic mixture). Different salt treatments on P. strombulifera plants influenced leaf micromorphological traits differently. In this study, Na2SO4-treated plants showed an increase in stomatal density (SD) and epidermal cell density (ECD) (with smaller stomata) at moderate and high salinity (−1.9 and −2.6 MPa), whereas in NaCl and NaCl + Na2SO4 treated plants, a decrease in these variables was observed. In Na2SO4-treated plants, transpiration was the highest at moderate and high salinity, with the highest content of ABA registered. A possible explanation is that, despite of these high ABA levels, there is no inhibition in stomatal opening, resulting in increased water loss, growth inhibition, and acceleration of senescence processes. We demonstrate that P. strombulifera responds to progressive salt stress by different salts changing the leaf development, particularly in Na2SO4-treated plants, leading to structural modifications in leaf size and micro-morphology of leaf cells.Fil: Reginato, Mariana Andrea. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular. Laboratorio de Fisiología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Reinoso, Herminda Elmira. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; ArgentinaFil: Llanes, Analia Susana. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular. Laboratorio de Fisiología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Luna, Maria Virginia. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular. Laboratorio de Fisiología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

Vínculos entre a filosofia e a didática: práticas de ensino nas escolas secundárias argentinas

This paper presents the advances of the theoretical framework of an ongoing research, aiming to analyze the didactic practices in two secondary schools from Entre Ríos, Argentina. The philosophical contributions of Arendt and Rancière are used to analyze the development and concretization of decisions about teaching and, particularly, how times are instituted for this practice in the school scenarios under study. Among the results presented as conclusions, it is pointed out that the conditions that traditionally determined secondary education underwent a series of transformations from which new practices emerged, characterized by the instability of the institutional organizational frameworks that, in the case of the analyzed schools, show their dependence on the responsiveness of the subjects involved.El artículo presenta avances del marco teórico de una investigación en curso, cuyo objetivo es analizar las prácticas didácticas en dos escuelas secundarias de Entre Ríos, Argentina. Los aportes filosóficos de Arendt y Rancière son usados para analizar cómo se gestan y se concretan decisiones sobre la enseñanza, en particular, cómo se instituyen los tiempos para esta práctica en los escenarios escolares estudiados. Entre los resultados que se presentan como conclusiones se señala que las condiciones que tradicionalmente organizaron la enseñanza media sufrieron transformaciones de las cuales surgieron nuevas prácticas caracterizadas por la inestabilidad de los marcos organizativos institucionales que, en el caso de las escuelas analizadas, evidencian su dependencia de la capacidad de respuesta de los sujetos involucrados.O artigo apresenta avanços do marco teórico de uma pesquisa em curso, cujo objetivo é analisar as práticas didáticas em duas escolas de secundária de Entre Rios, Argentina. As contribuições filosóficas de Arendt e Rancière são utilizadas para analisar como são conformadas e concretadas as decisões sobre o ensino, especificamente, como são instruídos os temos para esta prática nos cenários escolares estudados. Entre os resultados apresentados como conclusões, assinala-se que as condições que tradicionalmente organizaram o ensino na média sofreram transformações das que surgiram novas práticas caraterizadas pela instabilidade dos quadros organizativos institucionais que, no caso das escolas analisadas, evidenciam sua dependência da capacidade de resposta dos sujeitos envolvidos

Physiological responses of a halophytic shrub to salt stress by Na2SO4 and NaCl: oxidative damage and the role of polyphenols in antioxidant protection

Salt stress conditions lead to increased production of reactive oxygen species (ROS) in plant cells. Halophytes have the ability to reduce these toxic ROS by means of a powerful antioxidant system that includes enzymatic and non-enzymatic components. In this research, we used the halophytic shrub Prosopis strombulifera to investigate whether the ability of this species to grow under increasing salt concentrations and mixtures was related to the synthesis of polyphenolic compounds and to the maintenance of leaf pigment contents for an adequate photosynthetic activity. Seedlings of P. strombulifera were grown hydroponically in Hoagland's solution, gradually adding Na2SO4 and NaCl separately or in mixtures until reaching final osmotic potentials of −1, −1.9 and −2.6 MPa. Control plants were allowed to develop in Hoagland's solution without salt. Oxidative damage in tissues was determined by H2O2 and malondialdehyde content. Leaf pigment analysis was performed by high-performance liquid chromatography with ultraviolet, and total phenols, total flavonoids, total flavan-3-ols, condensed tannins, tartaric acid esters and flavonols were spectrophotometrically assayed. Treatment with Na2SO4 increased H2O2 production and lipid peroxidation in tissues and induced a sharp increase in flavonoid compounds (mainly flavan-3-ols) and consequently in the antioxidant activity. Also, Na2SO4 treatment induced an increased carotenoid/chlorophyll ratio, which may represent a strategy to protect photosystems against photooxidation. NaCl treatment, however, did not affect H2O2 content, lipid peroxidation, pigments or polyphenols synthesis. The significant accumulation of flavonoids in tissues under Na2SO4 treatment and their powerful antioxidant activity indicates a role for these compounds in counteracting the oxidative damage induced by severe salt stress, particularly, ionic stress. We demonstrate that ionic interactions between different salts in salinized soils modify the biochemical and morpho-physiological responses of P. strombulifera plants to salinity.Fil: Reginato, Mariana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquímicas y Naturales. Cátedra de Fisiología Vegetal; ArgentinaFil: Castagna, Antonella. Università degli Studi di Pisa; ItaliaFil: Furlan, Ana Laura. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; ArgentinaFil: Castro, Stella Maris. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; ArgentinaFil: Ranieri, Annamaria. Università degli Studi di Pisa; ItaliaFil: Luna, Maria Virginia. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquímicas y Naturales. Cátedra de Fisiología Vegetal; Argentin

Foliar application of phytohormones enhances growth of maize and soybean seedlings

Phytohormones such as gibberellins, auxins and cytokinins are plant growth promoting factors which added to foliar fertilizers can modulate plant growth and development of agricultural species. This work was performed to study the effects of exogenously applied phytohormones both alone and in mixtures, on a legume and a cereal growing in chambers with controlled conditions of humidity, temperature and light/dark cycle. It was found that application of phytohormones resulted in a considerable increase in growth of soybean and maize plants. The mixture of phytohormones formulated with the lowest concentration of each required to enhance plant growth, allowed a significant improvement on several growth parameters involved in productivity. Thus, the addition of this mixture to commercial products as foliar fertilizers may render potential improvement of legume and cereal yields.Las fitohormonas tales como giberelinas, auxinas y citoquininas son compuestos promotores del crecimiento que, adicionados a fertilizantes foliares, podrían contribuir a mejorar el crecimiento y desarrollo de la planta. Este trabajo fue realizado para evaluar los efectos de la aplicación de fitohormonas exógenas, tanto solas como en mezclas, en leguminosas y gramíneas creciendo en cámaras con condiciones controladas de humedad, temperatura y ciclos de luz/oscuridad. La aplicación de fitohormonas ocasionó una considerable mejora en el crecimiento de plantas de soja y maíz. El formulado a base de la mezcla de fitohormonas, cada una en la concentración mínima requerida para lograr un efecto cuantificable, permitió mejorar significativamente las variables de crecimiento consideradas de importancia para aumentar la productividad. Así, la adición de esta mezcla de fitohormonas a productos comerciales utilizados como fertilizantes para aplicaciones foliares podría mejorar el crecimiento y rendimiento en leguminosas y gramíneas.Gerencia de Comunicación e Imagen Institucional, DNA SICC, INTAFil: Llanes, Analía. Universidad de Río Cuarto. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Departamento de Ciencias Naturales. Laboratorio de Fisiología Vegetal; ArgentinaFil: Iparraguirre, Julia. Universidad de Río Cuarto. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Departamento de Ciencias Naturales. Laboratorio de Fisiología Vegetal; ArgentinaFil: Masciarelli, Oscar. Universidad de Río Cuarto. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Departamento de Ciencias Naturales. Laboratorio de Fisiología Vegetal; ArgentinaFil: María, N. Universidad de Río Cuarto. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Departamento de Ciencias Naturales. Laboratorio de Fisiología Vegetal; ArgentinaFil: Luna, Maria Virginia. Universidad de Río Cuarto. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Departamento de Ciencias Naturales. Laboratorio de Fisiología Vegetal; Argentin

Physiological and biochemical responses to drought stress and subsequent rehydration in the symbiotic association peanut-Bradyrhizobium sp

Drought stress is one of the most important environmental factors that regulate plant growth and development and limit its production. Peanut (Arachis hypogaea L.) is an agriculturally valuable plant with widespread distribution in the world serving as a subsistence food crop as well as a source of various food products. The aims of this work were to evaluate growth and nodulation as well as some physiological and biochemical stress indicators in response to drought stress and subsequent rehydration in the symbiotic association peanut-Bradyrhizobium sp. SEMIA6144. Drought stress affected peanut growth reducing shoot dry weight, nodule number, and dry weight as well as nitrogen content, but root dry weight increased reaching major exploratory surface. Besides, this severe water stress induced hydrogen peroxide production associated with lipid and protein damage; however, the plant was able to increase soluble sugar and abscisic acid contents as avoidance strategies to cope with drought stress. These physiological and biochemical parameters were completely reversed upon rehydration, in a short period of time, in the symbiotic association peanut-Bradyrhizobium sp. Thus, the results provided in this work constitute the initial steps of physiological and biochemical responses to drought stress and rehydration in this nodulated legume.Fil: Furlan, Ana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; ArgentinaFil: Llanes, Analia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; ArgentinaFil: Luna, Maria Virginia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Castro, Stella Maris. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; Argentin

Germination responses of lycium humile, an extreme halophytic Solanaceae: Understanding its distribution in saline mudflats of the southern puna

Although knowledge about halophytic Solanaceae is scarce, it is known that several species within genus Lycium tolerate salinity. Lycium humile grows in highly saline soils in mudflats near saline Andean lakes. This study evaluated the germination responses of L. humile under different scarification methods, photoperiods, temperatures and saline conditions and, simultaneously, tested seedling survival under different iso-osmotic conditions. Dormancy and germination were found to be regulated by interactions with different factors, with the highest germination percentages being obtained by immersion in sulfuric acid, with a temperature of 25 °C and a temperature regime of 5/25 °C, under which seeds were neutrally photoblastic. As osmotic potential of saline solutions decreased, germination also decreased drastically but the seedling survival percentage was higher than 30 % at 600 mM NaCl. No seeds germinated in any of the polyethylene glycol (PEG) solutions and no seedling survival was observed from-1.2 MPa PEG solutions. More than 90 % of seeds incubated in NaCl were able to recover germination after being transferred to distilled water, independently of NaCl treatments. We concluded that the effects of extreme environmental conditions on germination responses and seed tolerance to salinity may determine the occurrence and restricted distribution of L. humile.Fil: Palchetti, Maria Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Llanes, Analia Susana. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones Agrobiotecnológicas - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Agrobiotecnológicas; ArgentinaFil: Reginato, Mariana Andrea. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones Agrobiotecnológicas - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Agrobiotecnológicas; ArgentinaFil: Barboza, Gloria Estela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Luna, María Virginia. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones Agrobiotecnológicas - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Agrobiotecnológicas; ArgentinaFil: Cantero, Juan Jose. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentin

Growth enhancement and drought tolerance of hybrid poplar upon inoculation with endophyte consortia

With increasing effects of global climate change, there is a strong interest in developing biofuels from trees such as poplar (Populus sp.) that have high C sequestration rates and relatively low chemical inputs. Using plant-microbe symbiosis to maximize plant growth and increase host stress tolerance may play an important role in improving the economic viability and environmental sustainability of poplar as a feedstock. Based on our previous research, a total of ten endophyte strains were selected as a consortium to investigate the effects of inoculation on commercial hardwood cuttings of Populus deltoides x P. nigra clone OP-367. After one and a half months of growth under non-stress conditions followed by one month under water stress, there was substantial growth promotion with improved leaf physiology of poplar plants in response to the endophyte inoculation. Furthermore, inoculated plants demonstrated reduced damage by reactive oxygen species (ROS) indicating a possible mechanism for symbiosis-mediated drought tolerance. Production of important phytohormones by these endophytes and identification of microbial genes involved in conferring drought tolerance suggests their potential roles in the modulation of the plant host stress response.Fil: Khan, Zareen. University of Washington; Estados UnidosFil: Rho, Hyungmin. University of Washington; Estados UnidosFil: Firrincieli, Andrea. Università degli Studi della Tuscia; ItaliaFil: Hung, Shang Han. University of Washington; Estados UnidosFil: Luna, Maria Virginia. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Investigaciones Agrobiotecnologicas. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Investigaciones Agrobiotecnologicas.; ArgentinaFil: Masciarelli, Oscar Alberto. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Ciencias Naturales; ArgentinaFil: Kim, Soo-Hyung. University of Washington; Estados UnidosFil: Doty, Sharon L.. University of Washington; Estados Unido

Comparative transcriptomic analysis reveals novel roles of transcription factors and hormones during the flowering induction and floral bud differentiation in sweet cherry trees (Prunus avium L. Cv. Bing)

In sweet cherry trees, flowering is commercially important because the flowers, after fertilization, will generate the fruits. In P. avium, the flowering induction and flower organogensis are the first developmental steps towards flower formation and they occur within specialized organs known as floral buds during the summer, nine months before blooming. During this period the number of floral buds per tree and the bud fruitfulness (number of flowers per bud) are stablished affecting the potential yield of orchards and the plant architecture. The floral bud development is sensitive to any type of stress and the hotter and drier summers will interfere with this process and are calling for new adapted cultivars. A better understanding of the underlying molecular and hormonal mechanisms would be of help, but unlike the model plant Arabidopsis, very little is known about floral induction in sweet cherry. To explore the molecular mechanism of floral bud differentiation, high-throughput RNA sequencing was used to detect differences in the gene expression of P. avium floral buds at five differentiation stages. We found 2,982 differentially expressed genes during floral bud development. We identified genes associated with floral initiation or floral organ identity that appear to be useful biomarkers of floral development and several transcription factor families (ERF, MYB, bHLH, MADS-box and NAC gene family) with novel potential roles during floral transition in this species. We analyzed in deep the MADS-box gene family and we shed light about their key role during floral bud and organs development in P. avium. Furthermore, the hormonal-related signatures in the gene regulatory networks and the dynamic changes of absicic acid, zeatin and indolacetic acid contents in buds suggest an important role for these hormones during floral bud differentiation in sweet cherry. These data provide a rich source of novel informacion for functional and evolutionary studies about floral bud development in sweet cherry and new tools for biotechnology and breeding.Fil: Villar, Luis. Comision Nacional de Investigacion Cientifica y Tecnologica. Centro de Investigacion Regional. Centro de Estudios Avanzados En Fruticultura.; ChileFil: Lienqueo, Ixia. Comision Nacional de Investigacion Cientifica y Tecnologica. Centro de Investigacion Regional. Centro de Estudios Avanzados En Fruticultura.; ChileFil: Llanes, Analia Susana. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones Agrobiotecnológicas - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Agrobiotecnológicas; ArgentinaFil: Rojas, Pamela. Comision Nacional de Investigacion Cientifica y Tecnologica. Centro de Investigacion Regional. Centro de Estudios Avanzados En Fruticultura.; ChileFil: Perez, Jorge. Comision Nacional de Investigacion Cientifica y Tecnologica. Centro de Investigacion Regional. Centro de Estudios Avanzados En Fruticultura.; ChileFil: Correa, Francisco. Comision Nacional de Investigacion Cientifica y Tecnologica. Centro de Investigacion Regional. Centro de Estudios Avanzados En Fruticultura.; ChileFil: Sagredo, Boris. Comision Nacional de Investigacion Cientifica y Tecnologica. Centro de Investigacion Regional. Centro de Estudios Avanzados En Fruticultura.; ChileFil: Masciarelli, Oscar Alberto. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones Agrobiotecnológicas - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Agrobiotecnológicas; ArgentinaFil: Luna, Maria Virginia. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones Agrobiotecnológicas - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Agrobiotecnológicas; ArgentinaFil: Almada, Rubén. Comision Nacional de Investigacion Cientifica y Tecnologica. Centro de Investigacion Regional. Centro de Estudios Avanzados En Fruticultura.; Chil

Trends in crop reference evapotranspiration and climatological variables across Ceará state: Brazil.

Irrigation has a substantial share in total world water demand. At the global level, the withdrawal ratio for agriculture is 69 percent. Irrigation is necessary to compensate evapotranspiration (ET) deficit due to insufficient precipitation. Knowing the impacts of climatic changes on meteorological variables that directly affect the ET is important for successful climate adaptation. This paper analyzes annual trends in measured meteorological variables and in the crop reference evapotranspiration (ET0), at eight climatological stations in Ceará State, Brazil. Two statistical tests for trend analysis were used - Mann-Kendall and linear regression. The results indicate positive trend, statistically significant, in the maximum air temperature in five of eight stations. Minimum air temperature showed positive trend in three stations. Wind speed, sunshine hours and relative humidity presented positive and negative trends. These irregular patterns directly impacted ET0 in three stations. It seems that the increasing trend in ET0 was probably due to a significant increase detected in maximum temperature and minimum air temperature, not fully offset by the decrease in wind speed and relative humidity. The warning from these results is that water demand for irrigation is expected to significantly increase over the next decades on in Jaguaribe River Basin

Galectin-1 expression imprints a neurovascular phenotype in proliferative retinopathies and delineates responses to anti-VEGF

Neovascular retinopathies are leading causes of irreversible blindness. Although vascular endothelial growth factor (VEGF) inhibitors have been established as the mainstay of current treatment, clinical management of these diseases is still limited. As retinal impairment involves abnormal neovascularization and neuronal degeneration, we evaluated here the involvement of galectin-1 in vascular and non-vascular alterations associated with retinopathies, using the oxygen-induced retinopathy (OIR) model. Postnatal day 17 OIR mouse retinas showed the highest neovascular profile and exhibited neuro-glial injury as well as retinal functional loss, which persisted until P26 OIR. Concomitant to VEGF up-regulation, galectin-1 was highly expressed in P17 OIR retinas and it was mainly localized in neovascular tufts. In addition, OIR induced remodelling of cell surface glycophenotype leading to exposure of galectin-1-specific glycan epitopes. Whereas VEGF returned to baseline levels at P26, increased galectin-1 expression persisted until this time period. Remarkably, although anti-VEGF treatment in P17 OIR improved retinal vascularization, neither galectin-1 expression nor non-vascular and functional alterations were attenuated. However, this functional defect was partially prevented in galectin-1-deficient (Lgals1-/-) OIR mice, suggesting the importance of targeting both VEGF and galectin-1 as non-redundant independent pathways. Supporting the clinical relevance of these findings, we found increased levels of galectin-1 in aqueous humor from patients with proliferative diabetic retinopathy and neovascular glaucoma. Thus, using an OIR model and human samples, we identified a role for galectin-1 accompanying vascular and non-vascular retinal alterations in neovascular retinopathies.Fil: Ridano, Magali Evelin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Subirada Caldarone, Paula Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Paz, Maria Constanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Lorenc, Valeria Erika. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Stupirski, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Gramajo, Ana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Unidad Ejecutora Instituto de Nanociencia y Nanotecnología. Unidad Ejecutora Instituto de Nanociencia y Nanotecnología - Nodo Bariloche | Comisión Nacional de Energía Atómica. Unidad Ejecutora Instituto de Nanociencia y Nanotecnología. Unidad Ejecutora Instituto de Nanociencia y Nanotecnología - Nodo Bariloche; ArgentinaFil: Luna, José Domingo. Clinica de Ojos Romagosa, Fundacion Ver; ArgentinaFil: Croci Russo, Diego Omar. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Rabinovich, Gabriel Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad Nacional de Córdoba. Facultad de Cs.exactas Físicas y Naturales. Departamento de Química. Cátedra de Química Biologica; ArgentinaFil: Garcia Sanchez, Maria Candela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentin