1,536 research outputs found
Vacuolar ATPase depletion contributes to dysregulation of endocytosis in bloodstream forms of Trypanosoma brucei
BACKGROUND
Vacuolar H-ATPase (V-ATPase) is a highly conserved protein complex which hydrolyzes ATP and pumps protons to acidify vacuolar vesicles. Beyond its role in pH maintenance, the involvement of V-ATPase in endocytosis is well documented in mammals and plants but is less clear in Trypanosoma brucei.
METHODS
In this study, the subcellular localization of V-ATPase subunit B (TbVAB) of T. brucei was assessed via in situ N-terminal YFP-tagging and immunofluorescence assays. Transgenic bloodstream forms (BSF) of T. brucei were generated which comprised either a V-ATPase subunit B (TbVAB) conditional knockout or a V-ATPase subunit A (TbVAA) knockdown. Acridine orange and BCECF-AM were employed to assess the roles of V-ATPase in the pH regulation of BSF T. brucei. The endocytic activities of three markers were also characterized by flow cytometry analyses. Furthermore, trypanosomes were counted from trypanolysis treatment groups (either containing 1% or 5% NHS) and endocytosed trypanosome lytic factor (TLF) was also analyzed by an immunoblotting assay.
RESULTS
TbVAB was found to localize to acidocalcisomes, lysosomes and probably also to endosomes of BSF of T. brucei and was demonstrated to be essential for cell growth. TbVAB depletion neutralized acidic organelles at 24 hours post-tetracycline depletion (hpd), meanwhile the steady state intracellular pH increased from 7.016 ± 0.013 to 7.422 ± 0.058. Trypanosomes with TbVAB depletion at 24 hpd were found to take up more transferrin (2.068 ± 0.277 fold) but less tomato lectin (49.31 ± 22.57%) by endocytosis, while no significant change was detected in dextran uptake. Similar endocytic dysregulated phenotypes were also observed in TbVAA knockdown cells. In addition, TbVAB depleted trypanosomes showed a low uptake of TLF and exhibited less sensitive to lysis in both 1% and 5% NHS treatments.
CONCLUSIONS
TbVAB is a key component of V-ATPase and was found to play a key function in endocytosis as well as exhibiting different effects in a receptor/cargo dependent manner in BSF of T. brucei. Besides vacuolar alkalinization, the dysregulation of endocytosis in TbVAB depleted T. brucei is considered to contribute to the reduced sensitivity to lysis by normal human serum
Dynamics of inelastically colliding rough spheres: Relaxation of translational and rotational energy
We study the exchange of kinetic energy between translational and rotational
degrees of freedom for inelastic collisions of rough spheres. Even if
equipartition holds in the initial state it is immediately destroyed by
collisions. The simplest generalisation of the homogeneous cooling state allows
for two temperatures, characterizing translational and rotational degrees of
freedom separately. For times larger than a crossover frequency, which is
determined by the Enskog frequency and the initial temperature, both energies
decay algebraically like with a fixed ratio of amplitudes, different
from one.Comment: 5 pages, RevTeX, 2 eps figures, slightly expanded discussion, new
figures with dimensionless units, added references, accepted for publication
in PRE as a Rapid Com
First and Second Sound Modes of a Bose-Einstein Condensate in a Harmonic Trap
We have calculated the first and second sound modes of a dilute interacting
Bose gas in a spherical trap for temperatures () and for
systems with to particles. The second sound modes (which exist
only below ) generally have a stronger temperature dependence than the
first sound modes. The puzzling temperature variations of the sound modes near
recently observed at JILA in systems with particles match
surprisingly well with those of the first and second sound modes of much larger
systems.Comment: a shorten version, more discussions are given on the nature of the
second sound. A long footnote on the recent work of Zaremba, Griffin, and
Nikuni (cond-mat/9705134) is added, the spectrum of the (\ell=1, n_2=0) mode
is included in fig.
Homogeneous cooling of rough, dissipative particles: Theory and simulations
We investigate freely cooling systems of rough spheres in two and three
dimensions. Simulations using an event driven algorithm are compared with
results of an approximate kinetic theory, based on the assumption of a
generalized homogeneous cooling state. For short times , translational and
rotational energy are found to change linearly with . For large times both
energies decay like with a ratio independent of time, but not
corresponding to equipartition. Good agreement is found between theory and
simulations, as long as no clustering instability is observed. System
parameters, i.e. density, particle size, and particle mass can be absorbed in a
rescaled time, so that the decay of translational and rotational energy is
solely determined by normal restitution and surface roughness.Comment: 10 pages, 10 eps-figure
Spinor Bose Condensates in Optical Traps
In an optical trap, the ground state of spin-1 Bosons such as Na,
K, and Rb can be either a ferromagnetic or a "polar" state,
depending on the scattering lengths in different angular momentum channel. The
collective modes of these states have very different spin character and spatial
distributions. While ordinary vortices are stable in the polar state, only
those with unit circulation are stable in the ferromagnetic state. The
ferromagnetic state also has coreless (or Skyrmion) vortices like those of
superfluid He-A. Current estimates of scattering lengths suggest that the
ground states of Na and Rb condensate are a polar state and a
ferromagnetic state respectively.Comment: 11 pages, no figures. email : [email protected]
Granular cooling of hard needles
We have developed a kinetic theory of hard needles undergoing binary
collisions with loss of energy due to normal and tangential restitution. In
addition, we have simulated many particle systems of granular hard needles. The
theory, based on the assumption of a homogeneous cooling state, predicts that
granular cooling of the needles proceeds in two stages: An exponential decay of
the initial configuration to a state where translational and rotational
energies take on a time independent ratio (not necessarily unity), followed by
an algebraic decay of the total kinetic energy . The simulations
support the theory very well for low and moderate densities. For higher
densities, we have observed the onset of the formation of clusters and shear
bands.Comment: 7 pages, 8 figures; major changes, extended versio
Spherically Symmetric Gravitational Collapse of General Fluids
We express Einstein's field equations for a spherically symmetric ball of
general fluid such that they are conducive to an initial value problem. We show
how the equations reduce to the Vaidya spacetime in a non-null coordinate
frame, simply by designating specific equations of state. Furthermore, this
reduces to the Schwarzschild spacetime when all matter variables vanish. We
then describe the formulation of an initial value problem, whereby a general
fluid ball with vacuum exterior is established on an initial spacelike slice.
As the system evolves, the fluid ball collapses and emanates null radiation
such that a region of Vaidya spacetime develops. Therefore, on any subsequent
spacelike slice there exists three regions; general fluid, Vaidya and
Schwarzschild, all expressed in a single coordinate patch with two
free-boundaries determined by the equations. This implies complicated matching
schemes are not required at the interfaces between the regions, instead, one
simply requires the matter variables tend to the appropriate equations of
state. We also show the reduction of the system of equations to the static
cases, and show staticity necessarily implies zero ``heat flux''. Furthermore,
the static equations include a generalization of the Tolman-Oppenheimer-Volkoff
equations for hydrostatic equilibrium to include anisotropic stresses in
general coordinates.Comment: 11 pages, 3 figures, submitted to Phys. Rev.
Sistem Informasi Strategis Mendayagunakan Sistem Informasi untuk Mencapai Keunggulan Kompetitif
While most companies are content to develop and implement computer-based information systems to improve their operational and managerial effectiveness, a few also rely on innovative systems to give them an edge over the competition. An information system that is specifically designed with the aim to create a competitive advantage for its firm is called a strategic information system.To build a strategic information system, one should have a good understanding as to what factors contribute towards competitive advantage. It is here that the analyses by Michael E. Porter on this subject are particularly invaluable. Furthermore, one should also realize what the modern computer excels at fast processing time, fast data retrieval, fast communications, and reliability.There are also risks inherent to strategic information systems. These include the large capital outlay involved, as well as the reality that any competitive advantage thus achieved is not sustainable for long
Is There Only One Solution of the Dyson-Schwinger Equation for Quark Propagator in the Case of Non-zero Current Quark Mass
In this letter it is shown on general ground that there exist two
qualitatively distinct solutions of the Dyson-Schwinger equation for the quark
propagator in the case of non-zero current quark mass. One solution corresponds
to the ``Nambu-Goldstone'' phase and the other one corresponds to the
``Wigner'' phase in the chiral limit.Comment: 7 page
Dual neutral variables and knot solitons in triplet superconductors
In this paper we derive a dual presentation of free energy functional for
spin-triplet superconductors in terms of gauge-invariant variables. The
resulting equivalent model in ferromagnetic phase has a form of a version of
the Faddeev model. This allows one in particular to conclude that spin-triplet
superconductors allow formation of stable finite-length closed vortices (the
knotted solitons).Comment: Replaced with version published in PRL (added a discussion of the
effect of the coupling of the fields {\vec s} and {\vec C} on knot
stability). Latest updates of the paper and miscellaneous links related to
knotted solitons are also available at the homepage of the author
http://www.teorfys.uu.se/PEOPLE/egor/ . Animations of knotted solitons by
Hietarinta and Salo are available at
http://users.utu.fi/h/hietarin/knots/c45_p2.mp
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