Multidrug resistance 1 gene polymorphisms may determine Crohn's disease behavior in patients from Rio de Janeiro

OBJECTIVES: Conflicting data from studies on the potential role of multidrug resistance 1 gene polymorphisms in inflammatory bowel disease may result from the analysis of genetically and geographically distinct populations. Here, we investigated whether multidrug resistance 1 gene polymorphisms are associated with inflammatory bowel diseases in patients from Rio de Janeiro. METHODS: We analyzed 123 Crohn's disease patients and 83 ulcerative colitis patients to determine the presence of the multidrug resistance 1 gene polymorphisms C1236T, G2677T and C3435T. In particular, the genotype frequencies of Crohn's disease and ulcerative colitis patients were analyzed. Genotype-phenotype associations with major clinical characteristics were established, and estimated risks were calculated for the mutations. RESULTS: No significant difference was observed in the genotype frequencies of the multidrug resistance 1 G2677T/A and C3435T polymorphisms between Crohn's disease and ulcerative colitis patients. In contrast, the C1236T polymorphism was significantly more common in Crohn's disease than in ulcerative colitis (p = 0.047). A significant association was also found between the multidrug resistance 1 C3435T polymorphism and the stricturing form of Crohn's disease (OR: 4.13; p = 0.009), whereas no association was found with penetrating behavior (OR: 0.33; p = 0.094). In Crohn's disease, a positive association was also found between the C3435T polymorphism and corticosteroid resistance/refractoriness (OR: 4.14; p = 0.010). However, no significant association was found between multidrug resistance 1 gene polymorphisms and UC subphenotypic categories. CONCLUSION: The multidrug resistance 1 gene polymorphism C3435T is associated with the stricturing phenotype and an inappropriate response to therapy in Crohn's disease. This association with Crohn's disease may support additional pathogenic roles for the multidrug resistance 1 gene in regulating gut-microbiota interactions and in mediating fibrosis. Understanding the effects of several drugs associated with multidrug resistance 1 gene variants may aid in the selection of customized therapeutic regimens

A ABORDAGEM DO FEMINICÍDIO E SUA FORMA DE REGISTRO: PROTOCOLO DE REVISÃO DE ESCOPO

Este artigo apresenta o protocolo de revisão de escopo acerca da abordagem do feminicídio e sua forma de registro. O protocolo tem por objetivo identificar e explorar a terminologia do feminicídio no contexto científico, por meio de um mapeamento das pesquisas provenientes da aplicação da metodologia de revisão de escopo, com objetivo secundário de investigar as características do sistema operacional de registro de dados e notificação, ou seja, as fontes de informação usadas na identificação de ocorrências de feminicídios, orientado pelas diretrizes do Joanna Briggs Institute (JBI). O protocolo da revisão foi elaborado seguindo os itens do PRISMA Extension for Scopings Reviews (PRISMA-ScR): Checklist and Explanation.O protocolo foi registrado no Open Science Framework

UBE2C Is a Transcriptional Target of the Cell Cycle Regulator FOXM1

FOXM1 (forkhead box protein M1) is a transcription factor that participates in all stages of tumor development, mainly through the control of cell cycle and proliferation, regulating the expression of genes involved in G1/S and G2/M transition and M phase progression. The ubiquitin conjugating enzyme E2 (UBE2C) is a member of the anaphase promoting complex/cyclosome, promoting the degradation of several target proteins along cell cycle progression, during metaphase/anaphase transition. FOXM1 and UBE2C have been found overexpressed in a wide range of different solid tumors. Therefore, the aim of this study was to investigate whether UBE2C is a transcriptional target of FOXM1, using esophageal squamous cell carcinoma (ESCC) as a model, in addition to several cancer-deposited data. Our results show that FOXM1 and UBE2C expression present a positive correlation in normal tissues and in 25 distinct tumor types, including ESCC, where these genes are overexpressed. Moreover, FOXM1 binds to UBE2C promoter region in ESCC cell line and transcriptionally activates it, leading to UBE2C upregulation. In conclusion, this study provides evidences that FOXM1 transcriptionally regulates UBE2C expression in ESCC and their deregulation may be a general phenomenon in human neoplasias

Respuestas cardiovasculares de mujeres con obesidad mórbida sometidas a un test ergoespirométrico con ergómetro de brazo

Introducción: la obesidad mórbida es una enfermedad de etiología multifactorial, de gran prevalencia en el mundo, que guarda relación directa con otras comorbilidades. Objetivo: analizar las respuestas cardiovasculares de pacientes mujeres con obesidad mórbida, a través de un test de esfuerzo máximo para la estratificación del riesgo y la prescripción de ejercicio. Método: se incluyeron trece pacientes con obesidad grado III, de sexo femenino, con edad entre 20 a 40 años, que fueron sometidas a un test ergoespirométrico en el cicloergómetro de miembros superiores para la verificación y el análisis de la frecuencia cardiaca, la presión arterial y el doble producto. Resultados: las pacientes con media de edad de 34,7 ± 5,1 años tenían índice de masa corporal de 46,5 ± 3,8 kg/m2. Alcanzaron una frecuencia cardíaca máxima de 168,2 ± 14,6 lpm, sin respuesta hipertensiva al esfuerzo, y presión arterial de 171,1 ± 22,2 mm Hg (sistólica) x 87,5 ± 4,2 mm Hg (diastólica). El doble producto máximo encontrado fue de 28.662,2 ±3.644,1 mm Hg.lpm. Conclusión: de acuerdo con los resultados de este estudio se observó una reducción de la frecuencia cardiaca, la presión arterial y el doble producto cardiaco, compatible con el esfuerzo esperado para el test