Distribuição espacial de dengue, chikungunya e Zika e os determinantes socioeconômicos em um município da Bahia

Introdução: desde 1996, o município de Feira de Santana, na Bahia, apresenta sucessivas epidemias de dengue, sendo as mais importantes, do ponto de vista de incidência da doença, as relatadas em 2002 e 2009, com elevados números de casos registrados, hospitalizações e óbitos. Em 2015, houve destaque no registro de casos das três arboviroses, sendo que além da dengue, observa-se a introdução da chikungunya em 2014 e da Zika em 2015. O índice alto de infestação do Aedes aegypti nas áreas urbana e rural do município pode ter contribuído para a dispersão do vírus.  Objetivo: analisar a distribuição espacial da incidência das três arboviroses nas áreas urbana (bairros) e rural (distritos) do município do estudo e avaliar a relação entre os casos das arboviroses e os determinantes socioeconômicos. Metodologia: estudo descritivo realizado em Feira de Santana, no período de 2009 a 2017. Os dados foram distribuídos com auxílio do QGIS por bairros e distritos, apresentados em mapas temáticos. Utilizou-se o programa estatístico Stata versão 14 para análise de dados. Resultados: o estudo mostrou que os bairros localizados na periferia do município e os distritos apresentaram as maiores incidências e as piores condições socioeconômicas, além da co-circulação das três arboviroses na mesma área geográfica e no mesmo período. Conclusão: faz-se necessário ter uma rede de diagnóstico e de vigilância epidemiológica e entomológica consolidada a fim de melhorar o controle destas arboviroses e aprimorar a assistência à saúde

ATLANTIC EPIPHYTES: a data set of vascular and non-vascular epiphyte plants and lichens from the Atlantic Forest

Epiphytes are hyper-diverse and one of the frequently undervalued life forms in plant surveys and biodiversity inventories. Epiphytes of the Atlantic Forest, one of the most endangered ecosystems in the world, have high endemism and radiated recently in the Pliocene. We aimed to (1) compile an extensive Atlantic Forest data set on vascular, non-vascular plants (including hemiepiphytes), and lichen epiphyte species occurrence and abundance; (2) describe the epiphyte distribution in the Atlantic Forest, in order to indicate future sampling efforts. Our work presents the first epiphyte data set with information on abundance and occurrence of epiphyte phorophyte species. All data compiled here come from three main sources provided by the authors: published sources (comprising peer-reviewed articles, books, and theses), unpublished data, and herbarium data. We compiled a data set composed of 2,095 species, from 89,270 holo/hemiepiphyte records, in the Atlantic Forest of Brazil, Argentina, Paraguay, and Uruguay, recorded from 1824 to early 2018. Most of the records were from qualitative data (occurrence only, 88%), well distributed throughout the Atlantic Forest. For quantitative records, the most common sampling method was individual trees (71%), followed by plot sampling (19%), and transect sampling (10%). Angiosperms (81%) were the most frequently registered group, and Bromeliaceae and Orchidaceae were the families with the greatest number of records (27,272 and 21,945, respectively). Ferns and Lycophytes presented fewer records than Angiosperms, and Polypodiaceae were the most recorded family, and more concentrated in the Southern and Southeastern regions. Data on non-vascular plants and lichens were scarce, with a few disjunct records concentrated in the Northeastern region of the Atlantic Forest. For all non-vascular plant records, Lejeuneaceae, a family of liverworts, was the most recorded family. We hope that our effort to organize scattered epiphyte data help advance the knowledge of epiphyte ecology, as well as our understanding of macroecological and biogeographical patterns in the Atlantic Forest. No copyright restrictions are associated with the data set. Please cite this Ecology Data Paper if the data are used in publication and teaching events. © 2019 The Authors. Ecology © 2019 The Ecological Society of Americ

Estudo do polimorfismo genético dos virus linfotrópicos de células-T humanas dos tipos I e II (HTLV-I e HTLV-II) em Salvador, Bahia, Brasil e em tribos indígenas brasileiras

Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2013-11-26T17:14:41Z No. of bitstreams: 1 Luiz Carlos Alcantara Junior Estudo...2002.pdf: 5220779 bytes, checksum: b03dd8ec1b6c884b8aa5fd2db703f677 (MD5)Made available in DSpace on 2013-11-26T17:14:41Z (GMT). No. of bitstreams: 1 Luiz Carlos Alcantara Junior Estudo...2002.pdf: 5220779 bytes, checksum: b03dd8ec1b6c884b8aa5fd2db703f677 (MD5)Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, BrasilPoucos estudos analisaram a diversidade genética de HTLV em Salvador que têm características sócio-demográficas similares a algumas cidades africanas e apresenta a mais elevada prevalência para o HTLV-I (1,35%) em doadores de sangue do Brasil. Nós investigamos a real prevalência nesta população, 1,76% (23/1385). As taxas de infecção foram 1,2% e 2,0% para homens e mulheres, respectivamente, que aumentou com a idade. Quando nós analisamos uma coorte constituída por gestantes, encontramos uma freqüência de 0,9% (57 de 6754). Através de análise filogenética de parte do gene LTR virai, nós investigamos na população geral, gestantes, indivíduos com HAM/TSP e UDIs os subtipos de HTLV-I circulantes em Salvador. Demonstramos que a maioria das amostras pertence ao grupo da América Latina, subgrupo A (subtipo a). RFLP de fragmentos gênicos da globina p em 34 destes indivíduos demonstrou que 29.4% dos cromossomos são do haplótipo tipo CAR (Banto), sugerindo que Salvador recebeu também africanos do sul da África durante o tráfico de escravos. Assim, nossos resultados corroboram as hipóteses de múltiplas introduções pós- Colombianas do subgrupo A em Salvador. Nós também analisamos o polimorfismo do HTLV-ll em UDIs de Salvador e demonstramos que a maioria deles está relacionada à variante molecular Brasileira do subtipo lia. Assim, demonstramos pela primeira vez no Brasil a presença de um subtipo 11a, de UDIs, relacionado aos isolados 11a da América do Norte/Europa. Para investigar aspectos da epidemiología molecular das infecções causadas pelos HTLV-1, HTLV- II, e HlV-1 em populações indígenas brasileiras, testamos 683 e 321 soros de índios das tribos Tiriyo e Waiampi, respectivamente. As infecções pelo HIV-1 e HTLV-ll foram detectadas com prevalências muito baixas entre os Tiriyos, enquanto somente HTLV-I estava presente, com baixa prevalência, entre o Waiampis. Análise filogenética do gene env do HTLV-ll isolado dos Tiriyos mostrou que estas seqüências se agrupam mais próximas dos isolados de HTLV-ll de UDIs que vivem em áreas urbanas do sul do Brasil, do que aos isolados da tribo Kayapo. Isto confirma que a maioria das cepas brasileiras de HTLV-lia compreende um grupo filogenético com um considerável grau de diversidade.Few studies have analyzed the genetic diversity of HTLV in Salvador that have sociodemographic characteristics similar to some African cities and presents the highest HTLV-I prevalence rate (1.35%) of the Brazil. We investigated the real HTLV-1 prevalence in the Salvador population and demonstrated the overall prevalence of 1.76% (23/1385). The Infection rates were respectively 1.2% and 2.0% for males and females. Specific seroprevalence shows a trend with increasing age. When we analyzed the pregnant women cohort, we found a frequency relatively high (57 of 6754 women). We also studied in other cohort the circulating HTLV-1 strains in Salvador and the phylogenetic analysis from part of the LTR fragments showed that most of the samples belongs to the Latin American cluster of the subgroup A (subtype a). The p-globin RFLP in 34 of these individuals demonstrated that 29,4% of the chromosomes are CAR-haplotype (Bantu) suggesting that Salvador also received slaves from South Africa in the Atlantic slave trade. In addition, our results support the hypotheses of multiple post- Colombian introductions of African HTLV-la strains in Salvador. We also analyzed the HTLV-11 polymorphism in IDUs from Salvador and demonstrated that the majority of them are related to the subtype I la Brazilian molecular variant. Interestingly, we demonstrated for the first time in Brazil the presence of a subtype lla, from IDUs, closely related to the isolates from North America/Europe. To investigate serological, epidemiological and molecular aspects of HTLV-1, HTLV- II, and HIV-1 infections in Amerindian populations in Brazil, we tested 683 and 321 sera from Tiriyo and Waiampi Indians respectively. Both HIV-1 and HTLV-11 infections were detected in very low prevalences among the Tiriyos while only HTLV-1 was present among the Waiampis, also in low prevalence. Phylogenetic analyze of HTLV-ll-env gene from Tiriyo Indians showed that these sequences cluster closer to HTLV-II isolates from IDUs living in urban areas of Southern Brazil than the isolates from another Brazilian tribe, the Kayapos. Our results confirm that most of the HTLV-1 la Brazilian strains comprise a phylogenetic group harboring a considerable degree of diversity

CD4+ T-cell Count may not be a Useful Strategy to Monitor Antiretroviral Therapy Response in HTLV-1/HIV Co-infected Patients

Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2018-06-14T18:09:26Z No. of bitstreams: 1 Vandormael_A CD4+T-cell....pdf: 818800 bytes, checksum: 636c1967fdf550be45175f85dbb24022 (MD5)Approved for entry into archive by Ana Maria Fiscina Sampaio ([email protected]) on 2018-06-14T18:09:45Z (GMT) No. of bitstreams: 1 Vandormael_A CD4+T-cell....pdf: 818800 bytes, checksum: 636c1967fdf550be45175f85dbb24022 (MD5)Made available in DSpace on 2018-06-14T18:09:45Z (GMT). No. of bitstreams: 1 Vandormael_A CD4+T-cell....pdf: 818800 bytes, checksum: 636c1967fdf550be45175f85dbb24022 (MD5) Previous issue date: 2017Wellcome Trust, UK (082384/Z/07/Z). The Hlabisa HIV Treatment and Care programme was made possible by the support of the United States Agency for International Development (USAID) and the President’s Emergency Plan. Research was supported by a South African Medical Research Council (MRC) Flagship grant (MRC-RFA-UFSP-01-2013/UKZN HIVEPI) and by Brazil’s Ciencia Sem Fronteiras Project from CNPQ.University of KwaZulu-Natal. Africa Health Research Institute. Durban, South Africa / University of KwaZulu-Natal. Nelson R Mandela School of Medicine. College of Health Sciences. Durban, South Africa.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.University of KwaZulu-Natal. Africa Health Research Institute. Durban, South Africa.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.University of Minnesota. Center for Infectious Disease and Microbiology Translational Research. Minnesota, USA.University of KwaZulu-Natal. Nelson R Mandela School of Medicine. College of Health Sciences. Durban, South Africa / University of KwaZulu-Natal. Centre for the AIDS Programme of Research in South Africa. Durban, South Africa.Background: HTLV-1/HIV co-infection is known to elevate the CD4+ T-cell counts of treatment-naïve persons. We investigated whether HTLV-1/HIV co-infected patients continued to have elevated CD4+ T-cell counts after developing virologic failure on antiretroviral therapy (ART). Methods: The data comes from a drug resistance study located in the KwaZulu-Natal province of South Africa. All participants (N=383) presented for repeated CD4+ T-cell count and HIV viral load level testing between January 2006 and March 2014. We used a random-coefficient model to estimate the change in CD4+ T-cell count and HIV viral load level by HTLV-1/HIV co-infection status over time, adjusting for age, sex, and duration of virologic failure. Results: HTLV-1/HIV co-infected participants (n=8) had higher CD4+ T-cell counts, with a positive difference of 117.2 cells/μL at the ART initiation date (p-value=0.001), 114.7 cells/μL (p-value<0.001) 12 months after this date, and 112.3 cells/μL (p-value=0.005) 24 months after this date, holding all else constant. In contrast, there was no difference in the HIV viral load level by HTLV-1/HIV co-infected status throughout the observation period. Conclusions: We show that HTLV-1/HIV co-infected participants continued to have elevated CD4+ T-cell counts after developing virologic failure on ART, despite no difference in their HIV viral load levels when compared with HIV mono-infected participants. Our results indicate that CD4+ T-cell count testing may not be a useful strategy to monitor ART response in the presence of HTLV-1/HIV co-infection

Prevalência da infecção pelo vírus linfotrópico humano de células T e fatores de risco associados à soropositividade em doadores de sangue da cidade de Rio Branco, AC, Brasil (1998-2001) / Seroprevalence of human T cell lymphotropic virus infection and associated factors of risk in blood donors of Rio Branco city, AC, Brazil (1998-2001)

Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2012-12-13T21:21:51Z No. of bitstreams: 1 Colin, Denise Duizit Prevalencia da ....pdf: 97854 bytes, checksum: 8a8ed086eb73c2df172c8d76d02d791a (MD5)Made available in DSpace on 2012-12-13T21:21:51Z (GMT). No. of bitstreams: 1 Colin, Denise Duizit Prevalencia da ....pdf: 97854 bytes, checksum: 8a8ed086eb73c2df172c8d76d02d791a (MD5) Previous issue date: 2002Fundação Hospital Estadual do Acre. Rio Branco, AC, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilCentro de Hematologia e Hemoterapia do Estado do Acre. Rio Branco, AC, BrasilUniversidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, BrasilPara cada doador de sangue soropositivo (ELISA, Abbott®) para HTLV-I/II, de dezembro de 1998 a março de 2001, também foram selecionados dois soronegativos. As amostras séricas foram re-testadas pelo ELISA (Murex®) e aquelas que permaneceram soropositivas foram testadas pelo Western Blot e pela PCR. Das 11.121 amostras séricas, 73 (0,66%) foram positivas (Abbott®), mas somente 12 (0,11%) permaneceram positivas (Murex®), enquanto que as 146 soronegativas foram confirmadas, apesar de ser sofrível o índice de concordância entre os dois ELISA. O Western Blot confirmou as 12 amostras como soropositivas: 8 (0,07%) HTLV-I; duas (0,02%) HTLV-II e duas (0,02%) indeterminadas – sendo pela PCR uma pelo HTLV-I e a outra pelo HTLV-II. Em conclusão, nessa população da Amazônia Ocidental foi muito baixa a soroprevalência de HTLV-I/II, apesar de ser esperada maior prevalência do HTLV-II devido a grande miscigenação racial indígena.Between December 1998 and March 2001, for each HTLV-I/II seropositive blood donor (ELISA, Abbott®), two HTLV-I/II seronegative blood donors were also chosen. The blood samples were re-tested by ELISA (Murex®), and those seropositives were also tested by Western Blot and PCR. Of the 11,121 blood samples, 73 (0.66%) were positives (Abbott®), but only 12 (0.11%) remained positives (Murex®), whereas the 146 seronegatives were confirmed, even though the concordance index between these two ELISA tests was hopeless. The Western Blot confirmed the twelve blood samples as seropositives: 8 (0.07%) HTLV-I; two (0.02%) HTLV-II and two (0.02%) indeterminate, being by PCR, one HTLV-I and the other HTLV-II. Concluding, in this Western Amazon population the HTLV-I/II seroprevalence was too low, in spite of the greater prevalence of HTLV-II expected due to a great indigenous racial mixture

A Fully Annotated Genome Sequence of Human T-Cell Lymphotropic Virus Type 1 (HTLV-1)

Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2017-09-25T18:10:38Z No. of bitstreams: 1 Barreto FK A fully annotated....pdf: 472001 bytes, checksum: 7713907ae6e4a766b43c82e6e14974c5 (MD5)Approved for entry into archive by Ana Maria Fiscina Sampaio ([email protected]) on 2017-09-25T18:22:35Z (GMT) No. of bitstreams: 1 Barreto FK A fully annotated....pdf: 472001 bytes, checksum: 7713907ae6e4a766b43c82e6e14974c5 (MD5)Made available in DSpace on 2017-09-25T18:22:35Z (GMT). No. of bitstreams: 1 Barreto FK A fully annotated....pdf: 472001 bytes, checksum: 7713907ae6e4a766b43c82e6e14974c5 (MD5) Previous issue date: 2017Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Bahian School of Medicine and Public Health. Salvador, BA, BrazilCatholic University of Salvador. Salvador, BA, BrazilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, BrasilIn the next generation of genome sequencing, sequence annotation plays an important role with respect to genome evaluation. The aim of annotation is to identify key features in the genome, such as genes and their products. Although annotation tools are available and some sequence features have been published, annotation information for many complete and partial genomes of Human T-Cell Lymphotropic Virus Type 1 (HTLV-1) remains unavailable from GenBank. Sequence analysis is critical to the understanding of the pathogenesis of HTLV-1, and a well-annotated reference sequence is an essential component in this analysis. More accurate and complete information about the HTLV-1 genome can assist the scientific community in investigations on possible therapeutic and prophylactic vaccines, as well as aid studies on the pathogenesis of HTLV-1-associated diseases. Here we describe for the first time the complete nucleotide position annotation of the frequently used HTLV-1 reference sequence, ATK1 (accession number: J02029.1)

Inferences about the global scenario of human T-cell lymphotropic virus type 1 infection using data mining of viral sequences

Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2014-06-04T17:50:24Z No. of bitstreams: 1 Araujo TH Inferences about....pdf: 432396 bytes, checksum: 7e7a0ebdfa463339e9afa88c8c9f9e45 (MD5)Made available in DSpace on 2014-06-04T17:50:24Z (GMT). No. of bitstreams: 1 Araujo TH Inferences about....pdf: 432396 bytes, checksum: 7e7a0ebdfa463339e9afa88c8c9f9e45 (MD5) Previous issue date: 2014Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, BrasilHuman T-cell lymphotropic virus type 1 (HTLV-1) is mainly associated with two diseases: tropical spastic paraparesis/HTLV-1-associated myelopathy (TSP/HAM) and adult T-cell leukaemia/lymphoma. This retrovirus infects five-10 million individuals throughout the world. Previously, we developed a database that annotates sequence data from GenBank and the present study aimed to describe the clinical, molecular and epidemiological scenarios of HTLV-1 infection through the stored sequences in this database. A total of 2,545 registered complete and partial sequences of HTLV-1 were collected and 1,967 (77.3%) of those sequences represented unique isolates. Among these isolates, 93% contained geographic origin information and only 39% were related to any clinical status. A total of 1,091 sequences contained information about the geographic origin and viral subtype and 93% of these sequences were identified as subtype “a”. Ethnicity data are very scarce. Regarding clinical status data, 29% of the sequences were generated from TSP/HAM and 67.8% from healthy carrier individuals. Although the data mining enabled some inferences about specific aspects of HTLV-1 infection to be made, due to the relative scarcity of data of available sequences, it was not possible to delineate a global scenario of HTLV-1 infection

Ethnic differences in the distribution of interleukin-6 polymorphisms among three brazilian ethnic groups

Submitted by Martha Silveira Berbert ([email protected]) on 2011-08-22T02:24:35Z No. of bitstreams: 1 R - Ethnic Differences in the Distribution of Interleukin-6.pdf: 245010 bytes, checksum: fdd52e0ea82c0c8195598f5d702c7bfa (MD5)Made available in DSpace on 2011-08-22T02:24:35Z (GMT). No. of bitstreams: 1 R - Ethnic Differences in the Distribution of Interleukin-6.pdf: 245010 bytes, checksum: fdd52e0ea82c0c8195598f5d702c7bfa (MD5) Previous issue date: 2005Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA. BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA. BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA. BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA. BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA. BrasilPolymorphisms in the interleukin-6 promoter region have been associated with diseases. In this study we investigated the -634G/C and -174G/C IL-6 promoter polymorphisms in three Brazilian ethnic groups. We verified that the allele frequencies of the two polymorphisms and haplotype frequencies varied significantly between the population

Diversidade genética do vírus da imunodeficiência humana tipo 1 (HIV-1) em mulheres infectadas de uma cidade do nordeste do Brasil / Genetic diversity of human immunodeficiency virus type-1 (HIV-1) in infected women from a northeast city of Brazil

Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2013-01-07T19:26:31Z No. of bitstreams: 1 Santos, Edson de souza et al. Genetic diversity of human....pdf: 136382 bytes, checksum: de52000efab95336cedacae15cd0a137 (MD5)Made available in DSpace on 2013-01-07T19:26:31Z (GMT). No. of bitstreams: 1 Santos, Edson de souza et al. Genetic diversity of human....pdf: 136382 bytes, checksum: de52000efab95336cedacae15cd0a137 (MD5) Previous issue date: 2009Secretaria de Saúde de Feira de Santana. Centro de Referência em DST/HIV/Aids. Feira de Santana, BA, Brasil / Fundação Bahiana para o Desenvolvimento das Ciências. Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Bahiana para o Desenvolvimento das Ciências. Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, BrasilOBJETIVO: descrever a diversidade genética dos isolados de HIV-1 de mulheres soropositivas acompanhadas em um centro de referência. MÉTODOS: estudo transversal, no qual foram incluídas 96 mulheres com dois testes sorológicos ELISA e um teste confirmatório Western Blot. Das amostras de sangue periférico, foram determinadas a carga viral pelo kit b-DNA e a contagem de linfócitos T CD4 e T CD8 pela citometria de fluxo excalibur. A extração e purificação do DNA pró-viral foi realizada pela reação em cadeia da polimerase (PCR), utilizando o kit QIAamp Blood (Qiagen Inc., Chatsworth, CA, USA). O sequenciamento da região pol foi realizado em 52 isolados com o (3100 Genetic Analyzer, Applied Biosystems Inc., Foster City, CA) e a genotipagem foi investigada pela ferramenta Rega (Rega Subtyping Tool). O padrão de resistência aos antirretrovirais (ARV) foi inferido pelo algoritmo do banco de dados Stanford HIV Resistance. Os estágios clínicos das participantes foram definidos como A, B ou C segundo os critérios do Center for Diseases Control (CDC). Para a análise estatística dos dados, foram utilizados os testes do χ2 para as variáveis categóricas e o teste t de Student para as variáveis numéricas. RESULTADOS: a média de idade da amostra, o tempo médio de doença e de tratamento foram: 33,7; 3,8 e 2,5 anos, respectivamente. A média da carga viral foi log10 2,3 cópias/mL; a dos linfócitos T CD4 e T CD8 foi 494,9 células/μL e 1126,4 células/μL. Sobre o estágio clínico, 30 mulheres estavam no estádio A, 47 no B e 19 no C. O sequenciamento dos 52 isolados encontrou 33 do subtipo B, quatro do F, um do C e 14 do recombinante BF. A análise da resistência aos ARV mostrou 39 (75,0%) isolados susceptíveis, 13 (25,0%) resistentes aos inibidores da transcriptase reversa (INTR) e três (5,7%) aos inibidores da protease (IP). CONCLUSÕES: Houve grande diversidade do HIV-1 e elevado percentual de isolados resistentes aos ARV na amostra estudada.PURPOSE: to describe the genetic diversity of HIV-1 isolates from serum positive women followed up at a reference center. METHODS: transversal study, including 96 women with two ELISA serological tests and a Western Blot confirmatory test. The viral charge was determined by the b-DNA kit, and the counting of T CD4 and T CD8 lymphocytes, by the Excalibur flow cytometry, from the samples of peripheral blood. The extraction and purification of pro-viral DNA was performed by the polymerase (PCR) chain reaction, using the QIAamp Blood kit (Qiagen Inc., Chatsworth, CA, U.S.A.). Sequencing of the pol region was done in 52 isolates with the 3100 Genetic Analyzer (Applied Biosystems Inc., Foster City, CA), and the genotyping was assessed by the Rega Subtyping Tool. The resistance pattern to anti-retrovirals (ARV) was inferred by the algorithm from the Stanford HIV Resistance data bank. Participants’ clinical stages were defined as A, B or C, according to the criteria established by the Center for Diseases Control (CDC). For statistical analysis, the χ2 test was used for the categorical variables and the Student’s t test, for the numerical variables. RESULTS: The average age of the sample, the disease and treatment average duration were respectively: 33.7 years old, 3.8 and 2.5 years. The viral charge average was log10 2.3 copies/mL; the T CD4 e T CD8 lymphocytes, 494.9 cells/μL and 1126.4 cells/μL. Concerning the clinical stage, 30 women were in stage A, 47 in B and 19 in C. Sequencing from the 52 isolates found 33 of B subtype, 4 of F, 1 of C and 14 of BF recombinant. The analysis of resistance to AR