Velocity of Ultrasonic Waves in Solutions of Electrolytes

List of the genes involved in CVDs according to DisGeNET database. For each gene all the related diseases and the putative EV-MiRNAs targeting it are indicated both as list and as number of occurrences. (PDF 1206 kb

Titanium and Zirconium Levels Are Associated with Changes in MicroRNAs Expression: Results from a Human Cross-Sectional Study on Obese Population

<div><p>Objectives</p><p>In this study on 90 individuals we aimed at evaluating the microRNAs (miRNAs) expression profile associated with personal levels of Titanium (Ti) and Zirconium (Zr) traced in hair samples. Ti and Zr materials are broadly used for dental implants but the biological reactions triggered by a long term presence of these materials in the oral cavity still need to be assessed. MiRNAs are mechanisms that need to be investigated as they play a fundamental role in the control of gene expression following external stimuli and contribute to a wide range of pathophysiological processes.</p><p>Methods</p><p>Using the TaqMan^® Low-Density Array, we assessed the expression levels of 377 human miRNAs in peripheral blood of 90 subjects. Hair samples were analyzed for Ti and Zr content using Inductively Coupled Plasma-Mass Spectrometry. We performed multivariable regression analysis to investigate the effects of Ti and Zr exposure on miRNA expression levels. We used the Ingenuity Pathway Analysis (IPA) software to explore the functional role of the investigated miRNAs and the related target genes.</p><p>Results</p><p>Seven miRNAs (miR-99b, miR-142-5p, miR-152, miR-193a-5p, miR-323-3p, miR-335, miR-494) resulted specifically associated with Zr levels. The functional target analysis showed that miRNAs are involved in mechanisms such as inflammation, skeletal and connective tissue disorders.</p><p>Conclusions</p><p>Our data suggest that Zr is more bioactive than Ti and show that miRNAs are relevant molecular mechanisms sensitive to Zr exposure.</p></div

Characteristics of the study participants and exposure levels (n = 90).

<p>Characteristics of the study participants and exposure levels (n = 90).</p

Disease and biological function associated with target genes selected with IPA for 7 miRNAs.

<p>Disease and biological function associated with target genes selected with IPA for 7 miRNAs.</p

List of miRNAs associated with Zirconium levels.

<p>List of miRNAs associated with Zirconium levels.</p

Cellular location of targets shared by miR-152, miR-335 and miR-494.

<p>Cellular location of targets shared by miR-152, miR-335 and miR-494.</p

Additional file 1: Figure S1. of Extracellular vesicle-packaged miRNA release after short-term exposure to particulate matter is associated with increased coagulation

Diagram describing the two-stage, split-sample study design for miRNA analysis. (PDF 307 kb