Regeneration of plants from apical meristem tips and nodal segments of Arachis pintoi

The in vitro regeneration potential of shoot apical tips (2 to 3 mm in length), meristems (0.3 to 0.5 mm in length), and nodal segments (4 to 7 mm long with an axillary bud) of diploid (2n = 2x = 20) and triploid (2n = 3x = 30) cytotypes of Arachis pintoi was evaluated. Explants were cultured on MS medium supplemented with different concentrations and combinations of naphthaleneacetic acid (NAA) and benzyladenine (BA). In one experiment the effect of gibberellic acid was tested. The cultures were done in liquid and solid media. Plant regeneration can be readily achieved from all explants in one step of 30 d culture on MS + 0.01 mg/L each of NAA and BA or two steps consisting of 1) shoots regeneration through culture of explants on MS + 0.01 mg/L each of NAA and BA, and 2) induction of rooting in regenerated shoots by reculture on MS + 0.01 mg/L NAA. The plantlets were successfully transferred to pots in a greenhouse.Fil: Rey de Badaró, Hebe Yolanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Mroginski, Luis Amado. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentin

Cryopreservation of plant germplasm in Argentina

This review describes the current status of development of methods for cryopreservation (at -196ºC) of plants germplasm in Argentina. Arachis pintoi, a forage legume, has been maintained as seeds using vitrification method. Additionally, apical meristems, shoot tips, and somatic embryos have been cryopreserved using encapsulation-dehydration. Zygotic embryos, encapsulated and dehydrated, have permitted the cryopreservation of seven species of the genus Ilex. Various explants (apical meristems, uninodal segments, buds and somatic embryos) of Melia azedarach have been cryopreserved using the encapsulation-dehydration method. Protocols based in encapsulation-dehydration have also been developed for shoot tips of Citrus sinensis, seeds and protocorms of Oncidum bifolium and anthers of Oryza sativa. Vitrification protocols have been developed forcryopreservation of shoot tips of Solanum tuberosum and seeds of Toona ciliata.Fil: Mroginski, Luis Amado. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Rey de Badaró, Hebe Yolanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentin

Organogenesis and plant regeneration of Arachis villosa Benth. (Leguminosae) through leaf culture

With the aim of developing an efficient plant regeneration protocol, leaflet explants of three accessions of Arachis villosa Benth. (S2866, S2867 and L97) were cultured on basic Murashige and Skoog medium supplemented with different combinations of plant growth regulators: α-naphthalenacetic acid, indole-3-butyric acid, 6-benzylaminopurine, kinetin and thidiazuron. The accession L97 was the only one able to differentiate buds through indirect organogenesis. The most suitable combination for bud regeneration was the basic medium added with 13.62 μM thidiazuron and 4.44 μM 6-benzylaminopurine. These results show the important role of the genotype in morphogenetic responses and the organogenetic effect of thidiazuron in Arachis villosa accession L97. A thidiazuron lacking media (only 0.54 μM α-naphthalenacetic acid, 13.95 μM kinetin and 13.32 μM 6-benzylaminopurine were added) promoted the elongation of the regenerated buds. Adventitious rooting was achieved 90 days after the isolated shoots were transferred to a rooting medium containing 0.54 μM α-naphthalenacetic acid.Fil: Fontana, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Mroginski, Luis Amado. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Rey de Badaró, Hebe Yolanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentin

About speedup improvement of classical genetic algoritms using CUDA environment

Due to the increasing computational cost required for the numerical solution of evolutionary systems and problems based on topological design, in the last years, many parallel algorithms have been developed in order to improve its performance. Perhaps, the main numerical tool used to solve heuristic problems is known as Genetic Algorithm (GA), deriving its name from the similarity to the evolutionary theory of Darwing. During the last decade, Graphic Processing Unit (GPU) has been used for computing acceleration due to the intrinsic vector-oriented design of the chip set. This gave race to a new programming paradigm: the General Purpose Computing on Graphics Processing Units (GPGPU). Which was replaced then by the Compute Unified Device Architecture (CUDA) environment in 2007. CUDA environment is probably the parallel computing platform and programming model that more heyday has had in recent years, mainly due to the low acquisition cost of the graphics processing units (GPUs) compared to a cluster with similar functional characteristics. Consequently, the number of GPU-CUDAs present in the top 500 fastest supercomputers in the world is constantly growing. In this work, a numerical algorithm developed in the NVIDIA CUDA platform capable of solving classical optimization functions usually employed as benchmarks (De Jong, Rastring and Ackley functions) is presented. The obtained results using a GeForce GTX 750 Ti GPU shown that the proposed code is a valuable tool for acceleration of GAs, improving its speedup in about 130%.Fil: Mroginski, Javier Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional del Nordeste. Facultad de Ingeniería; ArgentinaFil: Castro, Hugo Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Modelado e Innovación Tecnológica. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Modelado e Innovación Tecnológica; ArgentinaXXII Congreso de Métodos Numéricos y sus AplicacionesCórdobaArgentinaUniversidad Tecnológica Nacional. Facultad Regional CórdobaAsociación Argentina de Mecánica Computaciona

A metaheuristic optimization algorithm for multimodal benchmark function in a GPU architecture

It is well known that the numerical solution of evolutionary systems and problems based on topological design requires a high computational power. In the last years, many parallel algorithms have been developed in order to improve its performance. Among them, genetic algorithms (GAs) are one of the most popular metaheuristic algorithms inspired by Darwin´s evolution theory. From the High Performance Computing (HPC) point of view, the CUDA environment is probably the parallel computing platform and programming model that more heyday has had in recent years, mainly due to the low acquisition cost of graphics processing units (GPUs) compared to a cluster with similar functional characteristics. Consequently, the number of GPU-CUDAs present in the top 500 fastest supercomputers in the world is constantly growing. In this paper, a numerical algorithm developed in the NVIDIA CUDA platform capable of solving classical optimization functions usually employed as benchmarks is presented. The obtained results demonstrate that GPUs are a valuable tool for acceleration of GAs and may enable its use in much complex problems. Also, a sensitivity analysis is carried out in order to show the relative weight of each GA operator in the whole computational cost of the algorithm.Fil: Mroginski, Javier Luis. Universidad Nacional del Nordeste. Facultad de Ingeniería; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaFil: Castro, Hugo Guillermo. Universidad Tecnológica Nacional. Facultad Reg. Resistencia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Modelado e Innovación Tecnológica. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Modelado e Innovación Tecnológica; Argentin

Veinticinco años con el cultivo de tejidos de leguminosas

En la presente comunicación se detallan las investigaciones llevadas a cabo por el autor en el estudio de cultivos de leguminosas.Contiene bibliografíaAcademia Nacional de Agronomía y Veterinari

Enhanced seed germination of Ilex dumosa R. (Aquifoliaceae) through in vitro culture of cut pyrenes

An in vitro culture protocol was developed that increased the germination percentage and decreased the lag time to germination for Ilex dumosa R. pyrenes as a tool for replacing the laborious task of embryo rescue technique. This method involves transversely cutting surface-sterilized pyrenes with a scalpel blade, then placing the micropylar one-third end with the rudimentary embryo (0.25 mm long) on solidified (agar 0.65%) quarter-strength salts and vitamins of Murashige and Skoog, 1962 medium with 3% sucrose, and incubating in a growth room at 27 ± 2 8C with a 14-h photoperiod (116 mmolm?2 s?1). Most of the cut pyrenes (greater than 50%) germinated within the first month after inoculation and achieved maximum germination (70%) in 2 months compared with whole pyrenes, which began to germinate 3 months after sowing and required more than 8 months for maximum germination (37%). Moreover, the germination percentage of cut pyrenes was significantly higher than the germination of isolated embryos (34%). Thus, the cut pyrenes culture is a simpler and more effective technique than embryo rescue. Easily, on average, a trained operator is able to culture ~1000 cut pyrenes per day instead of ~100 isolated embryos.Fil: Dolce, Natalia Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; ArgentinaFil: Mroginski, Luis Amado. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; ArgentinaFil: Rey, Hebe Yolanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentin

Preliminaries for a new mathematical framework for modelling tumour growth using stress state decomposition technique

The main goal of the present paper is to present a mathematical framework for modelling tumour growth based on stress state decomposition technique (SSDT). This is a straightforward extension of the model for multi-phase non-saturated soil consolidation with pollutant transport presented by the authors and may be regarded as an alternative to classical frameworks based on TCAT theory. In this preliminary work, the Representative Volume Element (RVE) for tumour is proposed along with its comparison with the corresponding one for soils modelling developed formerly by the authors. Equations standing for tumour phase are flawlessly brought into correspondence with those of gaseous phase in the soil problem showing that a similar task may be carried out for the remainders phases taking part in both RVEs. Furthermore, stresses induced by nonlinear saturation and permeability dependence on suction for soil interstitial fluids transport finds its counterpart on the contact between the cancer cell membrane and interstitial fluids rendering a higher primary variables coupling degree than what was attained in TCAT theory. From these preliminaries assessments, it may be put forward that likewise the stress state decomposition procedure stands for an alternative for modelling multi-phase nonsaturated soil consolidation with pollutant transport; it does for modelling cancer as well.Fil: Di Rado, Hector Ariel. Universidad Nacional del Nordeste. Facultad de Ingeniería; ArgentinaFil: Beneyto, Pablo Alejandro. Universidad Nacional del Nordeste. Facultad de Ingeniería; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Modelado e Innovación Tecnológica. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Modelado e Innovación Tecnológica; ArgentinaFil: Mroginski, Javier Luis. Universidad Nacional del Nordeste. Facultad de Ingeniería; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentin

Embryogenic cell suspensions from different explants and cultivars of Eragrostis curvula (Schrad.) Nees

The aim of this work was the establishment of embryogenic calli and cell suspensions from different explants and cultivars ofweeping lovegrass, Eragrostis curvula (Schrad.) Nees, to be used as targets for biolistic transformation. Calli were initiated from immature inflorescences, seeds, embryos, leafbases and root tips. Modified MS medium (Murashige and Skoog, 1962) was used for calli induction and proliferation. Cell suspensions were established and maintained in AAF medium (Wang et al., 1993). Morphogenic calli, embryogenic cell suspensions of moderate growth rate - consisting mainly of compact proembryogenic cell c1usters- and green plants were obtained from all the explants and cultivars assayed, except root tips. Both, explant and genotype were very important factors to be considered in order to obtain a morphogenic response and to establish cell suspensions from this grass. The statistical analysis detected interaction between both factors, explants and genotypes. Immature inflorescences were the best source of explant and Kromdraai was the cultivar that showed the best morphogenic response (expressed as the percentage of calli/explant and the percentage of calli with green spots -every green spot developed into green plants-) with inflorescences, embryos and leafbases. For Morpa and Don Pablo embryos as explants were less responsive than seeds and leafbases. There were no differences in leafbases for all the three cultivars analysed.Fil: Echenique, Carmen Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Díaz, Marina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; ArgentinaFil: Polci, Pablo Aldo. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Mroginski, Luis Amado. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentin

Thermodynamic gradient-based poroplastic theory for concrete under high temperatures

Concrete materials subjected to long term exposures to high temperatures suffer severe degradations in its mechanical properties (cohesion, friction, strength and stiffness) and changes in their failure mechanisms. These degradations may lead to irreversible damage or sudden collapse of the related structures. From the predictive analysis stand point, accurate constitutive theories are required to simulate the variations of concrete mechanical failure behavior under high and durable temperature fields. In the realm of the smeared crack approach, non-local model strategies are required to objectively reproduce failure behaviors under coupled thermo-mechanical loading conditions, while realistic descriptions of the involved characteristic lengths are needed to objectively reproduce the variation from ductile to brittle failure modes depending on the acting confining pressure and temperature. In this work, a thermodynamically consistent gradient poroplastic model for concrete subjected to high temperatures is proposed. A particular and simple form of gradient-based poroplasticity is considered whereby the state variables are the only ones of non-local character. The degradations of these variables due to coupled thermo-mechanical effects are described in the framework of the thermodynamic approach. After describing the material formulation, numerical analyses are presented which demonstrate the predictive capabilities of the proposed constitutive theory for different stress paths and thermal conditions.Fil: Ripani, Marianela. Universidad de Buenos Aires. Facultad de Ingenieria. Departamento de Construcciones y Estructuras. Laboratorio de Materiales y Estructuras; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Etse, Jose Guillermo. Universidad de Buenos Aires. Facultad de Ingenieria. Departamento de Construcciones y Estructuras. Laboratorio de Materiales y Estructuras; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Vrech, Sonia Mariel. Universidad de Buenos Aires. Facultad de Ingenieria. Laboratorio de Metodos Numericos En Ingenieria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Mroginski, Javier Luis. Universidad de Buenos Aires. Facultad de Ingenieria. Laboratorio de Metodos Numericos En Ingenieria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin