26 research outputs found

    Evaluación de la capacidad probiótica de una cepa de Lactobacillus murinus y su uso en el tratamiento de diarreas virales en caninos

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    Los probioticos son microorganismos vivos que administrados en cantidades adecuadas, confieren beneficios a la salud del huésped. Su uso clínico toma cada vez más importancia en la medida que se van comprobando sus efectos tanto en salud como en enfermedad del hombre y los animales dejando de ser paulatinamente una terapia alternativa para convertirse en parte de la terapéutica convencional. Para poder ser clasificado como probiótico, el microrganismo debe cumplir determinados requisitos in vitro e in vivo para lograr resultados específicos. Microorganismos provenientes de las microbiotas nativas serían útiles en el restablecimiento del equilibrio interno ya que se encuentran adaptados al medio sobre el cual se pretende actuar. En el presente trabajo se empleó la cepa de Lactobacillus murinus LbP2 aislada previamente de heces de caninos para ser evaluada como potencial probiótico. El objetivo general fue evaluar la persistencia en el medio intestinal, su efecto sobre parámetros generales, su capacidad probiótica por medio de la modulación de IgA fecal y su efecto en el tratamiento de las diarreas virales por Distemper. En términos generales, los resultados indicaron que la cepa en cuestión presenta propiedades como probiótico y que ejerció efectos beneficiosos sobre las poblaciones de caninos empleadas en el estudio. En el futuro de evaluarán aspectos adicionales vinculados al potencial probiótico de L. murinus LbP2 con el fin de avalar su administración en caninos

    Sinopsis de las Asteraceae de la Provincia de Catamarca

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    Fil: Freire, Susana Edith. Instituto de Botánica Darwinion. Buenos Aires; ArgentinaFil: Bayón, Néstor D.. Área de Botánica, Departamento de Ciencias Biológica Facultad de Ciencias Agrarias y Forestales. Universidad Nacional de La Plata; ArgentinaFil: Monti, Claudia. Área de Botánica. Departamento de Ciencias Biológica. Facultad de Ciencias Agrarias y Forestales. Universidad Nacional de La Plata; ArgentinaFil: Giuliano, Daniel A.. Área de Botánica. Departamento de Ciencias Biológica. Facultad de Ciencias Agrarias y Forestales. Universidad Nacional de La Plata; ArgentinaFil: Ariza Espinar, Luis. Museo Botánico. Facultad de Ciencias Exactas, Físicas y Naturales. Universidad Nacional de Córdoba; ArgentinaFil: Sáenz, Alcides A.. División Plantas Vasculares. Facultad de Ciencias Naturales y Museo. Universidad Nacional de La Plata; ArgentinaFil: Perea, Mario V.. Facultad de Ciencias Exactas y Naturales (UNCA). San Fernando del Valle de Catamarca; ArgentinaFil: Delucchi, Gustavo. División Plantas Vasculares. Facultad de Ciencias Naturales y Museo. Universidad Nacional de La Plata; Argentin

    New records of Asteraceae for the province of Catamarca (Argentina)

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    Se mencionan los siguientes nuevos registros de Asteráceas para la provincia de Catamarca, Argentina: Baccharis acaulis, B. boliviensis var. latifolia, B. flexuosa, B. glutinosa var. angustissima, B. stenophylla (Tribu Astereae), Eupatorium patens var. tomentosum (Tribu Eupatorieae), Cosmos bipinnatus, C. peucedanifolius var. peucedanifolius, Helianthus annuus (Tribu Heliantheae), Gamochaeta erythractis, G. longipedicellata, Gnaphalium lacteum, Tessaria integrifolia var. integrifolia (Tribu Inuleae), Chuquiraga ruscifolia (Tribu Mutisieae), Senecio crepidifolius, S. subulatus var. subulatus, Xenophyllum incisum var. incisum (Tribu Senecioneae). Se citan además el hábitat de los taxones y las provincias fitogeográficas que integran. Se propone una nueva combinación, Baccharis glutinosa Pers. var. angustissima (DC.) Giuliano.The following Asteraceae are mentioned for the province of Catamarca: Baccharis acaulis, B. boliviensis var. latifolia, B. flexuosa, B. glutinosa var. angustissima, B. stenophylla (Tribe Astereae), Eupatorium patens var. tomentosum (Tribe Eupatorieae), Cosmos bipinnatus, C. peucedanifolius var. peucedanifolius, Helianthus annuus (Tribe Heliantheae), Gamochaeta erythractis, G. longipedicellata, Gnaphalium lacteum, Tessaria integrifolia var. integrifolia (Tribe Inuleae), Chuquiraga ruscifolia (Tribe Mutisieae), Senecio crepidifolius, S. subulatus var. subulatus, Xenophyllum incisum var. incisum (Tribe Senecioneae). The habitat and phytogeographic provinces of the taxa are mentioned. The following new combination is proposed: Baccharis glutinosa Pers. var. angustissima (DC.) Giuliano.Facultad de Ciencias Agrarias y ForestalesFacultad de Ciencias Naturales y Muse

    Trastornos de salud mental en docentes bonaerenses

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    El sistema de revisión en juntas médicas de los docentes bonaerenses con trastornos de salud transcurrió por un período de insuficiente actividad. Ante esas circunstancias que generaban importante disfunción institucional, el Ministerio de Educación de la Provincia de Buenos Aires convenió con la Facultad de Ciencias Médicas la realización de las juntas médicas. En este poster se presentan resultados preliminares de los hallazgos durante la ejecución del convenio.Facultad de Ciencias Médica

    Distribution and conservation status of Phytolacca tetramera (Phytolaccaceae), a rare endemic plant of the Argentine Pampas

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    Introducción y objetivos: El ombusillo, Phytolacca tetramera, es una planta rara, endémica del noreste de la provincia de Buenos Aires, Argentina. El objetivo de este trabajo es proporcionar información detallada y actualizada del área de distribución de la especie, evaluar su estado de conservación y discutir las amenazas para su conservación. M&M: Los sitios de ocurrencia de la especie se obtuvieron a partir de material de herbario, bases de datos, bibliografía y viajes de campo. Delimitamos núcleos geográficos (potenciales subpoblaciones) para los grupos de ombusillos. Relevamos las posibles amenazas mediante observaciones a campo y evaluamos el estado de conservación usando el criterio B1 de la UICN (extensión de presencia). Resultados: Phytolacca tetramera está restringida en la actualidad al litoral del Río de la Plata entre las cotas de 4 y 15 msnm, desde el partido de La Plata hasta la Bahía Samborombón. Posee una extensión de presencia de 4313,714 km2, en los cuales se identificaron cuatro núcleos geográficos. De acuerdo con el criterio B1 de la UICN, proponemos la categoría En Peligro B1ab(i,iii) para P. tetramera. Las amenazas a la supervivencia de la especie son diversas, aunque deben ser mejor estudiadas, siendo la urbanización una de las más importantes. Conclusiones: Debido a la extensión geográfica restringida de P. tetramera y a sus posibles amenazas, resulta imprescindible llevar a cabo acciones para la conservación de esta especie. Dentro de las acciones prioritarias a futuro es fundamental conocer más sobre su capacidad de dispersión y nicho de regeneración.Background and aims: The “ombusillo”, Phytolacca tetramera, is a rare plant endemic to the northeast of Buenos Aires province, Argentina. The objective of this work is to provide a more accurate approximation of its distribution area, to evaluate the conservation status of the species and to list the threats to its conservation. M&M: The occurrence sites were obtained from herbarium specimens, databases, bibliography, and fieldwork. We delimited geographic cores, or potential subpopulations, for groups of “ombusillos”. We surveyed the activities that threaten the survival of the species and finally the conservation status was evaluated using the IUCN criterion B1 (extent of occurrence). Results: Phytolacca tetramera is currently restricted to the coast of the Río de la Plata between 4 and 15 m asl, from the district of La Plata to the Samborombón Bay. It has an extension of presence of 4313.714 km2, in which four geographic cores were identified. In accordance with IUCN criterion B1, we propose the category Endangered B1ab(i,iii) for P. tetramera. The threats to the survival of the species are diverse and need further studies, urbanization being one of the most serious. Conclusions: Due to the restricted geographical extension of P. tetramera and its possible threats, it is essential to carry out actions for the conservation of this species. Among the priority actions for the future, it is essential to know more about its dispersal capacity and regeneration niche.Facultad de Ciencias Naturales y Muse

    Cortisol secretion after adrenocorticotrophin (ACTH) and Dexamethasone tests in healthy female and male dogs

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    <p>Abstract</p> <p>Background</p> <p>For the conclusive diagnosis of Cushing's Syndrome, a stimulating ACTH test or a low suppressive Dexamethasone test is used. Reports in other species than the dog indicate that plasma cortisol concentration after ACTH administration is affected by gender. We investigated the effect of gender on the cortisol response to ACTH and Dexamethasone tests in dogs.</p> <p>Methods</p> <p>Seven healthy adult Cocker Spaniels (4 females and 3 males) were assigned to a two by two factorial design: 4 dogs (2 females and 2 males) received IV Dexamethasone 0.01 mg/kg, while the other 3 dogs received an IV saline solution (control group). Two weeks later the treatments were reversed. After one month, ACTH was given IV (250 μg/animal) to 4 dogs (2 female and 2 males) while the rest was treated with saline solution (control group). Cortisol concentrations were determined by a direct solid-phase radioimmunoassay and cholesterol and triglycerides by commercial kits.</p> <p>Results and Discussion</p> <p>No effect of treatment was observed in metabolite concentrations, but females presented higher cholesterol concentrations. ACTH-treated dogs showed an increase in cortisol levels in the first hour after sampling until 3 hours post injection. Cortisol concentrations in Dexamethasone-treated dogs decreased one hour post injection and remained low for 3 hours, thereafter cortisol concentrations increased. The increase in cortisol levels from one to two hours post ACTH injection was significantly higher in females than males. In Dexamethasone-treated males cortisol levels decreased one hour post injection up to 3 hours; in females the decrease was more pronounced and prolonged, up to 5 hours post injection.</p> <p>Conclusion</p> <p>We have demonstrated that cortisol response to ACTH and Dexamethasone treatment in dogs differs according to sex.</p

    Electroencephalographic signatures of dogs with presumptive diagnosis of canine cognitive dysfunction

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    Canine cognitive dysfunction (CCD) is a highly prevalent neurodegenerative disease considered the canine analog of early Alzheimer's disease (AD). Unfortunately, CCD cannot be cured. However, early therapeutic interventions can slow the progression of cognitive decline and improve quality of life of the patients; therefore, early diagnosis is ideal. In humans, electroencephalogram (EEG) findings specific to AD have been described, and some of them have successfully detect early stages of the disease. In this study we characterized the EEG correlates of CCD, and we compared them with the EEGs of healthy aging dogs and dogs at risk of developing CCD. EEG recordings were performed in 25 senior dogs during wakefulness. Dogs were categorized in normal, at risk of CCD or with CCD according to their score in the Rofina questionnaire. We demonstrated that, quantitative EEG can detect differences between normal dogs and dogs with CCD. Dogs with CCD experience a reduction in beta and gamma interhemispheric coherence, and higher Joint Lempel Ziv complexity. Dogs at risk of developing CCD, had higher alpha power and interhemispheric coherence, making these features potential markers of early stages of the disease. These results demonstrate that quantitative EEG analysis could aid the diagnosis of CCD, and reinforce the CCD as a translational model of early AD.Fil: Mondino, Alejandra. Universidad de la República; UruguayFil: Gutiérrez, Mary. Universidad de la República; UruguayFil: González, Camila. Universidad de la República; UruguayFil: Mateos, Diego Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Matemática Aplicada del Litoral. Universidad Nacional del Litoral. Instituto de Matemática Aplicada del Litoral; Argentina. Universidad Autónoma de Entre Ríos; ArgentinaFil: Torterolo, Pablo. Universidad de la República; UruguayFil: Olby, Natasha. North Carolina State University; Estados UnidosFil: Delucchi, Luis. Universidad de la República; Urugua

    Use of doramectin for treatment of notoedric mange in five cats

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    Nuevas citas de Asteraceae para la provincia de Catamarca (Argentina) New records of Asteraceae for the province of Catamarca (Argentina)

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    Se mencionan los siguientes nuevos registros de Asteráceas para la provincia de Catamarca, Argentina: Baccharis acaulis, B. boliviensis var. latifolia, B. flexuosa, B. glutinosa var. angustissima, B. stenophylla (Tribu Astereae), Eupatorium patens var. tomentosum (Tribu Eupatorieae), Cosmos bipinnatus, C. peucedanifolius var. peucedanifolius, Helianthus annuus (Tribu Heliantheae), Gamochaeta erythractis, G. longipedicellata, Gnaphalium lacteum, Tessaria integrifolia var. integrifolia (Tribu Inuleae), Chuquiraga ruscifolia (Tribu Mutisieae), Senecio crepidifolius, S. subulatus var. subulatus, Xenophyllum incisum var. incisum (Tribu Senecioneae). Se citan además el hábitat de los taxones y las provincias fitogeográficas que integran. Se propone una nueva combinación, Baccharis glutinosa Pers. var. angustissima (DC.) Giuliano.<br>The following Asteraceae are mentioned for the province of Catamarca: Baccharis acaulis, B. boliviensis var. latifolia, B. flexuosa, B. glutinosa var. angustissima, B. stenophylla (Tribe Astereae), Eupatorium patens var. tomentosum (Tribe Eupatorieae), Cosmos bipinnatus, C. peucedanifolius var. peucedanifolius, Helianthus annuus (Tribe Heliantheae), Gamochaeta erythractis, G. longipedicellata, Gnaphalium lacteum, Tessaria integrifolia var. integrifolia (Tribe Inuleae), Chuquiraga ruscifolia (Tribe Mutisieae), Senecio crepidifolius, S. subulatus var. subulatus, Xenophyllum incisum var. incisum (Tribe Senecioneae). The habitat and phytogeographic provinces of the taxa are mentioned. The following new combination is proposed: Baccharis glutinosa Pers. var. angustissima (DC.) Giuliano

    Comparison of cyclosporine concentrations 2 hours post-dose determined using 3 different methods and trough level in pediatric renal transplantation

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    Immunosuppression has been one of the great challenges in pediatric recipients of kidney allografts. Cyclosporine (CsA) has evolved during the past 25 years of transplantation. It requires frequent blood level monitoring because of its narrow therapeutic window and interpatient and intrapatient variability. Neoral (Novartis) is no exception. Ideally, monitoring of blood levels should also include determination of the area under the time-concentration curve (AUC) to better target the therapeutic window, thus avoiding underdosing or overdosing, especially in pediatric patients. A single blood concentration measurement 2 hours after Neoral administration (C2) has been shown to be a more for accurate predictor of drug exposure than trough levels (CO). Therefore, its use may lead to reduction in the incidence and severity of cellular rejection and of CsA toxicity. Some studies have shown that the metabolites/CsA ratio is substantially lower using C2 than CO, however, the between-assay differences for C2 monitoring have not been considered. The purpose of this study was to evaluate CsA C0 and C2 levels, determined using monoclonal fluorescence polarization immunoassay (FPIA)/TDx and enzyme multiplied immunoassay (EMIT). CsA levels were determined using a radioimmunoassay (RIA) in 30 pediatric transplant recipients with stable renal function within 42.7 mean months follow-up. Mean age was 13.4 years; 15 children were girls; 23 patients were recipients of cadaveric kidneys. The mean CsA microemulsion dose was 5.7 mg/kg/d. The 3 methods showed a high correlation between C0 and C2 (r >= 0.97). A linear regression slope was significantly higher for C0 than C2 (P < .001). The CsA concentrations both at C0 and C2 were significantly higher with FPIA than with RIA (P < .009) but no differences were found for EMITT (P = .2). The mean C0 level for FPIA was 22% and 26% higher than RIA and EMIT, respectively. The mean C2, for FPIA was 7% and 12% higher than RIA and EMIT, respectively. In conclusion, CsA levels determined using RIA or EMIT are better than using FPIA/Tx; also, C2 CsA levels are more accurate than C0 in pediatric transplantation patients
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