Cryo-FIB Machining: An Alternative to TEM Cryo-Sections Cut with Diamonds?

Extended abstract of a paper presented at Microscopy and Microanalysis 2011 in Nashville, Tennessee, USA, August 7-August 11, 201

Enhanced Auditory Brainstem Response and Parental Bonding Style in Children with Gastrointestinal Symptoms

The electrophysiological properties of the brain and influence of parental bonding in childhood irritable bowel syndrome (IBS) are unclear. We hypothesized that children with chronic gastrointestinal (GI) symptoms like IBS may show exaggerated brainstem auditory evoked potential (BAEP) responses and receive more inadequate parental bonding. = 0.024). Multiple regression analysis in females also supported these findings.It is suggested that children with chronic GI symptoms have exaggerated brainstem responses to environmental stimuli and inadequate parental behaviors aggravate these symptoms

Nitrate radicals and biogenic volatile organic compounds: Oxidation, mechanisms, and organic aerosol

Oxidation of biogenic volatile organic compounds (BVOC) by the nitrate radical (NO3) represents one of the important interactions between anthropogenic emissions related to combustion and natural emissions from the biosphere. This interaction has been recognized for more than 3 decades, during which time a large body of research has emerged from laboratory, field, and modeling studies. NO3-BVOC reactions influence air quality, climate and visibility through regional and global budgets for reactive nitrogen (particularly organic nitrates), ozone, and organic aerosol. Despite its long history of research and the significance of this topic in atmospheric chemistry, a number of important uncertainties remain. These include an incomplete understanding of the rates, mechanisms, and organic aerosol yields for NO3-BVOC reactions, lack of constraints on the role of heterogeneous oxidative processes associated with the NO3 radical, the difficulty of characterizing the spatial distributions of BVOC and NO3 within the poorly mixed nocturnal atmosphere, and the challenge of constructing appropriate boundary layer schemes and non-photochemical mechanisms for use in state-of-The-Art chemical transport and chemistry-climate models. This review is the result of a workshop of the same title held at the Georgia Institute of Technology in June 2015. The first half of the review summarizes the current literature on NO3-BVOC chemistry, with a particular focus on recent advances in instrumentation and models, and in organic nitrate and secondary organic aerosol (SOA) formation chemistry. Building on this current understanding, the second half of the review outlines impacts of NO3-BVOC chemistry on air quality and climate, and suggests critical research needs to better constrain this interaction to improve the predictive capabilities of atmospheric models.The authors acknowledge support from the International Global Atmospheric Chemistry project (IGAC), the US National Science Foundation (NSF grants AGS-1541331 and AGS-1644979), and Georgia Tech College of Engineering and College of Sciences for support of the workshop on nitrate radicals and biogenic hydrocarbons that led to this review article. N. L. Ng acknowledges support from NSF CAREER AGS-1555034 and US Environmental Protection Agency STAR (Early Career) RD-83540301. S. S. Brown acknowledges support from the NOAA Atmospheric Chemistry, Carbon Cycle and Climate program. A. T. Archibald and B. Ouyang thank NERC for funding through NE/M00273X/1. E. Atlas acknowledges NSF grant AGS-0753200. R. C. Cohen acknowledges NSF grant AGS-1352972. J. N. Crowley acknowledges the Max Planck Society. J. L. Fry, D. A. Day, and J. L. Jimenez acknowledge support from the NOAA Climate Program Office’s AC4 program, award no. NA13OAR4310063 (Colorado)/NA13OAR4310070 (Reed). N. M. Donahue acknowledges NSF AGS-1447056. M. I. Guzman wishes to acknowledge support from NSF CAREER award (CHE-1255290). J. L. Jimenez and D. A. Day acknowledge support from NSF AGS-1360834 and EPA 83587701-0. R. McLaren acknowledges NSERC grant RGPIN/183982-2012. H. Herrmann, A. Tilgner, and A. Mutzel acknowledge the DARK KNIGHT project funded by DFG under HE 3086/25-1. B. Picquet-Varrault acknowledges support from the French National Agency for Research (project ONCEM-ANR-12-BS06-0017-01). R. H. Schwantes acknowledges NSF AGS-1240604. Y. Rudich and S. S. Brown acknowledge support from the USA-Israel Binational Science Foundation (BSF) grant no. 2012013. Y. Rudich acknowledges support from the Henri Gutwirth Foundation. J. Mao acknowledges support from the NOAA Climate Program Office grant no. NA13OAR4310071. J. A. Thornton acknowledges support from NSF AGS 1360745. B. H. Lee was supported by the NOAA Climate and Global Change Postdoctoral Fellowship. R. A. Zaveri acknowledges support from the US Department of Energy (DOE) Atmospheric System Research (ASR) program under contract DE-AC06-76RLO 1830 at Pacific Northwest National Laboratory

The discovAIR project: a roadmap towards the Human Lung Cell Atlas

The Human Cell Atlas (HCA) consortium aims to establish an atlas of all organs in the healthy human body at single-cell resolution to increase our understanding of basic biological processes that govern development, physiology and anatomy, and to accelerate diagnosis and treatment of disease. The Lung Biological Network of the HCA aims to generate the Human Lung Cell Atlas as a reference for the cellular repertoire, molecular cell states and phenotypes, and cell–cell interactions that characterise normal lung homeostasis in healthy lung tissue. Such a reference atlas of the healthy human lung will facilitate mapping the changes in the cellular landscape in disease. The discovAIR project is one of six pilot actions for the HCA funded by the European Commission in the context of the H2020 framework programme. discovAIR aims to establish the first draft of an integrated Human Lung Cell Atlas, combining single-cell transcriptional and epigenetic profiling with spatially resolving techniques on matched tissue samples, as well as including a number of chronic and infectious diseases of the lung. The integrated Human Lung Cell Atlas will be available as a resource for the wider respiratory community, including basic and translational scientists, clinical medicine, and the private sector, as well as for patients with lung disease and the interested lay public. We anticipate that the Human Lung Cell Atlas will be the founding stone for a more detailed understanding of the pathogenesis of lung diseases, guiding the design of novel diagnostics and preventive or curative interventions