245 research outputs found

    Elegant sufficiency: how young Australians are living better with less

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    This research explores Australian consumer society and how the pursuit of materialist lifestyles had led to a problem of wasteful consumerism. This problem amounts to an annual expenditure of $10.5 billion on goods that are not used (Hamilton et. al, 2005). Despite the urgent need for society to mitigate environmental catastrophe and shift towards a sustainable way of living, the problem of wasteful consumerism is only increasing. This research explores why this is the case through an analysis of meaning and motivation for modern consumerism. To address the tension between the problem of wasteful consumerism and sustainability, this research analyses the emerging discourse on sustainable consumption lifestyles and identifies how this theory can translate to individual action. Furthermore, this research identifies how some young people are discontent with the consumerist status quo and as such, are seeking to adopt alternative, sustainable lifestyles. To gain insights into the sustainable lifestyles of young people, fourteen individuals committed to sustainable living (aged 18-35 years) are interviewed in-depth. Their stories are analysed and constructed into lifestyle portraits. The fourteen lifestyle portraits address the three objectives for this research and identify (1) the individual motivations for living sustainably, (2a) how young people are living sustainably, through an analysis of the principles and practices they adopt and (2b) the challenges between the individuals’ consuming ideals and actions. Finally, (3) the biographical analysis explores the spheres of influence the individuals have within their families, peers and broader networks and assesses their potential to lead by example through living better with less

    Evaluation of enzyme immunoassays in the diagnosis of camel (Camelus dromedarius) trypanosomiasis:a preliminary investigation

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    Three enzyme immunoassays were used for the serodiagnosis of Trypanosoma evansi in camels in the Sudan in order to evaluate their ability to discriminate between infected and non-infected animals. Two assays were used for the detection of trypanosomal antibodies, one using specific anti-camel IgG conjugate and another using a non-specific Protein A conjugate. The third assay detected the presence of trypanosomal antigens using anti-T. evansi antibodies in a double antibody sandwich assay. Inspection of the frequency distribution of assay results suggested that the ELISA for circulating trypanosomal antibodies using specific antisera and the ELISA for circulating antigens can distinguish between non-infected camels and infected camels exhibiting patent infections or not. The ELISA using Protein A conjugate to bind non-specifically to camel immunoglobulin did not appear to discriminate between infected and non-infected animals

    The effect of pore-scale contaminant distribution on the reactive decontamination of porous media

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    A porous material that has been contaminated with a hazardous chemical agent is typically decontaminated by applying a cleanser solution to the surface and allowing the cleanser to react into the porous material, neutralising the agent. The agent and cleanser are often immiscible fluids and so, if the porous material is initially saturated with agent, a reaction front develops with the decontamination reaction occurring at this interface between the fluids. We investigate the effect of different initial agent configurations within the pore space on the decontamination process. Specifically, we compare the decontamination of a material initially saturated by the agent with the situation when, initially, the agent only coats the walls of the pores (referred to as the ‘agent-on-walls’ case). In previous work (Luckins et al., European Journal of Applied Mathematics, 31(5):782–805, 2020), we derived homogenised models for both of these decontamination scenarios, and in this paper we explore the solutions of these two models. We find that, for an identical initial volume of agent, the decontamination time is generally much faster for the agent-on-walls case compared with the initially saturated case, since the surface area on which the reaction can occur is greater. However for sufficiently deep spills of contaminant, or sufficiently slow reaction rates, decontamination in the agent-on-walls scenario can be slower. We also show that, in the limit of a dilute cleanser with a deep initial agent spill, the agent-on-walls model exhibits behaviour akin to a Stefan problem of the same form as that arising in the initially saturated model. The decontamination time is shown to decrease with both the applied cleanser concentration and the rate of the chemical reaction. However, increasing the cleanser concentration is also shown to result in lower decontamination efficiency, with an increase in the amount of cleanser chemical that is wasted

    <i>Trypanosoma evansi</i>: Genetic variability detected using amplified restriction fragment length polymorphism (AFLP) and random amplified polymorphic DNA (RAPD) analysis of Kenyan isolates

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    We compared two methods to generate polymorphic markers to investigate the population genetics of Trypanosoma evansi; random amplified polymorphic DNA (RAPD) and amplified restriction fragment length polymorphism (AFLP) analyses. AFLP accessed many more polymorphisms than RAPD. Cluster analysis of the AFLP data showed that 12 T.evansi isolates were very similar (‘type A’) whereas 2 isolates differed substantially (‘type B’). Type A isolates have been generally regarded as genetically identical but AFLP analysis was able to identify multiple differences between them and split the type A T. evansi isolates into two distinct clades

    The role of temperature and drying cycles on impurity deposition in drying porous media

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    We consider a liquid containing impurities saturating a porous material; when the liquid evaporates, the impurities are deposited within the material. Applications include filtration and waterproof textiles. We present a mathematical model incorporating coupling between evaporation, accumulation and transport of the impurities, and the impact of the deposited impurities on the transport of both the suspended impurities and the liquid vapour. By simulating our model numerically, we investigate the role of temperature and repeated drying cycles on the location of the deposited impurities. Higher temperatures increase the evaporation rate so that impurities are transported further into porous material before depositing than for lower temperatures. We quantify two distinct parameter regimes in which the material clogs: (i) the dry-clogging (high-temperature) regime, in which impurities are pushed far into the material before clogging, and (ii) the wet-clogging (high-impurity) regime, in which liquid becomes trapped by the clogging. Clogging restricts the extent to which drying time can be reduced by increasing the temperature

    The role of temperature and drying cycles on impurity deposition in drying porous media

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    We consider a liquid containing impurities saturating a porous material; when the liquid evaporates, the impurities are deposited within the material. Applications include filtration and waterproof textiles. We present a mathematical model incorporating coupling between evaporation, accumulation and transport of the impurities, and the impact of the deposited impurities on the transport of both the suspended impurities and the liquid vapour. By simulating our model numerically, we investigate the role of temperature and repeated drying cycles on the location of the deposited impurities. Higher temperatures increase the evaporation rate so that impurities are transported further into porous material before depositing than for lower temperatures. We quantify two distinct parameter regimes in which the material clogs: (i) the dry-clogging (high-temperature) regime, in which impurities are pushed far into the material before clogging, and (ii) the wet-clogging (high-impurity) regime, in which liquid becomes trapped by the clogging. Clogging restricts the extent to which drying time can be reduced by increasing the temperature

    Trypanosoma evansi in Indonesian buffaloes: evaluation of simple models of natural immunity to infection

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    Deterministic models were employed to investigate the biology of Trypanosoma evansi infection in the Indonesian buffalo. Models were fitted to two age-structured data sets of infection. The Susceptible-Infected-Susceptible (SIS) model was the best supported description of this infection, although the results of the analysis depended on the serological test used; the Tr7 Ag-ELISA was judged the most reliable indicator of infection. Estimated forces of infection increase with age from 1.2 to 2.0 acquisitions per buffalo per year. The buffaloes would clear infection in an estimated mean time period of 16.8 months (95% CIs: 12.5-25.9 months) since acquisition, either by drug treatment by owners or self-cure. A general discussion on the role of immunity in protozoan infections includes consideration that the fitted SIS model would be consistent with strain-specific immunity. The model may become a useful tool for the evaluation of control programmes

    Complete In Vitro Life Cycle of Trypanosoma congolense: Development of Genetic Tools

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    Trypanosoma congolense is a parasite responsible for severe disease of African livestock. Its life cycle is complex and divided into two phases, one in the tsetse fly vector and one in the bloodstream of the mammalian host. Molecular tools for gene function analyses in parasitic organisms are essential. Previous studies described the possibility of completing the entire T. congolense life cycle in vitro. However, the model showed major flaws including the absence of stable long-term culture of the infectious bloodstream forms, a laborious time-consuming period to perform the cycle and a lack of genetic tools. We therefore aimed to develop a standardized model convenient for genetic engineering. We succeeded in producing long-term cultures of all the developmental stages on long-term, to define all the differentiation steps and to finally complete the whole cycle in vitro. This improved model offers the opportunity to conduct phenotype analyses of genetically modified strains throughout the in vitro cycle and also during experimental infections

    Population Genetics of Trypanosoma evansi from Camel in the Sudan

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    Genetic variation of microsatellite loci is a widely used method for the analysis of population genetic structure of microorganisms. We have investigated genetic variation at 15 microsatellite loci of T. evansi isolated from camels in Sudan and Kenya to evaluate the genetic information partitioned within and between individuals and between sites. We detected a strong signal of isolation by distance across the area sampled. The results also indicate that either, and as expected, T. evansi is purely clonal and structured in small units at very local scales and that there are numerous allelic dropouts in the data, or that this species often sexually recombines without the need of the “normal” definitive host, the tsetse fly or as the recurrent immigration from sexually recombined T. brucei brucei. Though the first hypothesis is the most likely, discriminating between these two incompatible hypotheses will require further studies at much localized scales

    Multiple evolutionary origins of Trypanosoma evansi in Kenya

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    Trypanosoma evansi is the parasite causing surra, a form of trypanosomiasis in camels and other livestock, and a serious economic burden in Kenya and many other parts of the world. Trypanosoma evansi transmission can be sustained mechanically by tabanid and Stomoxys biting flies, whereas the closely related African trypanosomes T. brucei brucei and T. b. rhodesiense require cyclical development in tsetse flies (genus Glossina) for transmission. In this study, we investigated the evolutionary origins of T. evansi. We used 15 polymorphic microsatellites to quantify levels and patterns of genetic diversity among 41 T. evansi isolates and 66 isolates of T. b. brucei (n = 51) and T. b. rhodesiense (n = 15), including many from Kenya, a region where T. evansi may have evolved from T. brucei. We found that T. evansi strains belong to at least two distinct T. brucei genetic units and contain genetic diversity that is similar to that in T. brucei strains. Results indicated that the 41 T. evansi isolates originated from multiple T. brucei strains from different genetic backgrounds, implying independent origins of T. evansi from T. brucei strains. This surprising finding further suggested that the acquisition of the ability of T. evansi to be transmitted mechanically, and thus the ability to escape the obligate link with the African tsetse fly vector, has occurred repeatedly. These findings, if confirmed, have epidemiological implications, as T. brucei strains from different genetic backgrounds can become either causative agents of a dangerous, cosmopolitan livestock disease or of a lethal human disease, like for T. b. rhodesiense
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