Assessment of Scale-Loss to Atlantic Salmon (Salmo Salar L.) Smolts from Passage Through an Archimedean Screw Turbine

The potential for external damage to Atlantic salmon (Salmo salar L.) smolts from passage through an Archimedean screw turbine was tested with controlled field trials at two turbine speeds. Change in external condition of smolts was measured by grading photographs of individual fish for scale-loss before and after the tests. Results were compared between turbine-passed and control smolts. There were no significant differences in proportions of fish with new scale-loss between treatment and control smolts. New scale-loss of between 4 and 30% was seen in 7.46% of turbine-passed smolts, exceeding the prevalence in control smolts by 2.46%. Of these, 1.49% had minor scale-loss of 5-9%. Minor scale-loss was more prevalent for both groups at the faster turbine speed, although differences between treatment and control groups were more apparent at the slower speed

Modelling of eddy currents related to large angle magnetic suspension test fixture

This report presents a preliminary analysis of the mathematical modelling of eddy current effects in a large-gap magnetic suspension system. It is shown that eddy currents can significantly affect the dynamic behavior and control of these systems, but are amenable to measurement and modelling. A theoretical framework is presented, together with a comparison of computed and experimental data related to the Large Angle Magnetic Suspension Test Fixture at NASA Langley Research Center

Extension to the dynamic modeling of the large angle magnetic suspension test fixture

The Large Angle Magnetic Suspension Test Fixture (LAMSTF) is a laboratory scale proof-of-concept system. The configuration is unique in that the electromagnets are mounted in a circular planar array. A mathematical model of the system had previously been developed, but was shown to have inaccuracies. These inaccuracies showed up in the step responses. Eddy currents were found to be the major cause of the modeling errors. In the original system, eddy currents existed in the aluminum baseplate, iron cores, and the sensor support frame. An attempt to include the eddy current dynamics in the system model is presented. The dynamics of a dummy sensor ring were added to the system. Adding the eddy current dynamics to the simulation improves the way it compares to the actual experiment. Also presented is a new method of determining the yaw angle of the suspended element. From the coil currents the yaw angle can be determined and the controller can be updated to suspend at the new current. This method has been used to demonstrate a 360 degree yaw angle rotation

Fluid Interactions That Enable Stealth Predation by the Upstream-Foraging Hydromedusa \u3cem\u3eCraspedacusta sowerbyi\u3c/em\u3e

Unlike most medusae that forage with tentacles trailing behind their bells, several species forage upstream of their bells using aborally located tentacles. It has been hypothesized that these medusae forage as stealth predators by placing their tentacles in more quiescent regions of flow around their bells. Consequently, they are able to capture highly mobile, sensitive prey. We used digital particle image velocimetry (DPIV) to quantitatively characterize the flow field around Craspedacusta sowerbyi, a freshwater upstream-foraging hydromedusa, to evaluate the mechanics of its stealth predation. We found that fluid velocities were minimal in front and along the sides of the bell where the tentacles are located. As a result, the deformation rates in the regions where the tentacles are located were low, below the threshold rates required to elicit an escape response in several species of copepods. Estimates of their encounter volume rates were examined on the basis of flow past the tentacles, and trade-offs associated with tentacle characteristics were evaluated

Mapping historical forest biomass for stock-change assessments at parcel to landscape scales

Understanding historical forest dynamics, specifically changes in forest biomass and carbon stocks, has become critical for assessing current forest climate benefits and projecting future benefits under various policy, regulatory, and stewardship scenarios. Carbon accounting frameworks based exclusively on national forest inventories are limited to broad-scale estimates, but model-based approaches that combine these inventories with remotely sensed data can yield contiguous fine-resolution maps of forest biomass and carbon stocks across landscapes over time. Here we describe a fundamental step in building a map-based stock-change framework: mapping historical forest biomass at fine temporal and spatial resolution (annual, 30m) across all of New York State (USA) from 1990 to 2019, using freely available data and open-source tools. Using Landsat imagery, US Forest Service Forest Inventory and Analysis (FIA) data, and off-the-shelf LiDAR collections we developed three modeling approaches for mapping historical forest aboveground biomass (AGB): training on FIA plot-level AGB estimates (direct), training on LiDAR-derived AGB maps (indirect), and an ensemble averaging predictions from the direct and indirect models. Model prediction surfaces (maps) were tested against FIA estimates at multiple scales. All three approaches produced viable outputs, yet tradeoffs were evident in terms of model complexity, map accuracy, saturation, and fine-scale pattern representation. The resulting map products can help identify where, when, and how forest carbon stocks are changing as a result of both anthropogenic and natural drivers alike. These products can thus serve as inputs to a wide range of applications including stock-change assessments, monitoring reporting and verification frameworks, and prioritizing parcels for protection or enrollment in improved management programs.Comment: Manuscript: 24 pages, 7 figures; Supplements: 12 pages, 5 figures; Submitted to Forest Ecology and Managemen

Establishing a malaria diagnostics centre of excellence in Kisumu, Kenya

<p>Abstract</p> <p>Background</p> <p>Malaria microscopy, while the gold standard for malaria diagnosis, has limitations. Efficacy estimates in drug and vaccine malaria trials are very sensitive to small errors in microscopy endpoints. This fact led to the establishment of a Malaria Diagnostics Centre of Excellence in Kisumu, Kenya. The primary objective was to ensure valid clinical trial and diagnostic test evaluations. Key secondary objectives were technology transfer to host countries, establishment of partnerships, and training of clinical microscopists.</p> <p>Case description</p> <p>A twelve-day "long" and a four-day "short" training course consisting of supervised laboratory practicals, lectures, group discussions, demonstrations, and take home assignments were developed. Well characterized slides were developed and training materials iteratively improved. Objective pre- and post-course evaluations consisted of 30 slides (19 negative, 11 positive) with a density range of 50–660 parasites/μl, a written examination (65 questions), a photographic image examination (30 images of artifacts and species specific characteristics), and a parasite counting examination.</p> <p>Discussion and Evaluation</p> <p>To date, 209 microscopists have participated from 11 countries. Seventy-seven experienced microscopists participated in the "long" courses, including 47 research microscopists. Sensitivity improved by a mean of 14% (CI 9–19%) from 77% baseline (CI 73–81 %), while specificity improved by a mean of 17% (CI 11–23%) from 76% (CI 70–82%) baseline. Twenty-three microscopists who had been selected for a four-day refresher course showed continued improvement with a mean final sensitivity of 95% (CI 91–98%) and specificity of 97% (CI 95–100%). Only 9% of those taking the pre-test in the "long" course achieved a 90% sensitivity and 95% specificity, which increased to 61% of those completing the "short" course. All measures of performance improved substantially across each of the five organization types and in each course offered.</p> <p>Conclusion</p> <p>The data clearly illustrated that false positive and negative malaria smears are a serious problem, even with research microscopists. Training dramatically improved performance. Quality microscopy can be provided by the Centre of Excellence concept. This concept can be extended to other diagnostics of public health importance, and comprehensive disease control strategies.</p

Minimal Change Disease Is Associated With Endothelial Glycocalyx Degradation and Endothelial Activation

Endothelial glycocalyx; Glomerular endothelial cell; Minimal change diseaseGlicocàlix endotelial; Cèl·lula endotelial glomerular; Malaltia de canvis mínimsGlicocálix endotelial; Célula endotelial glomerular; Enfermedad de cambios mínimosIntroduction Minimal change disease (MCD) is considered a podocyte disorder triggered by unknown circulating factors. Here, we hypothesized that the endothelial cell (EC) is also involved in MCD. Methods We studied 45 children with idiopathic nephrotic syndrome (44 had steroid sensitive nephrotic syndrome [SSNS], and 12 had biopsy-proven MCD), 21 adults with MCD, and 38 healthy controls (30 children, 8 adults). In circulation, we measured products of endothelial glycocalyx (EG) degradation (syndecan-1, heparan sulfate [HS] fragments), HS proteoglycan cleaving enzymes (matrix metalloprotease-2 [MMP-2], heparanase activity), and markers of endothelial activation (von Willebrand factor [vWF], thrombomodulin) by enzyme-linked immunosorbent assay (ELISA) and mass spectrometry. In human kidney tissue, we assessed glomerular EC (GEnC) activation by immunofluorescence of caveolin-1 (n = 11 MCD, n = 5 controls). In vitro, we cultured immortalized human GEnC with sera from control subjects and patients with MCD/SSNS sera in relapse (n = 5 per group) and performed Western blotting of thrombomodulin of cell lysates as surrogate marker of endothelial activation. Results In circulation, median concentrations of all endothelial markers were higher in patients with active disease compared with controls and remained high in some patients during remission. In the MCD glomerulus, caveolin-1 expression was higher, in an endothelial-specific pattern, compared with controls. In cultured human GEnC, sera from children with MCD/SSNS in relapse increased thrombomodulin expression compared with control sera. Conclusion Our data show that alterations involving the systemic and glomerular endothelium are nearly universal in patients with MCD and SSNS, and that GEnC can be directly activated by circulating factors present in the MCD/SSNS sera during relapse