Bioinformatics service reconciliation by heterogeneous schema transformation

This paper focuses on the problem of bioinformatics service reconciliation in a generic and scalable manner so as to enhance interoperability in a highly evolving field. Using XML as a common representation format, but also supporting existing flat-file representation formats, we propose an approach for the scalable semi-automatic reconciliation of services, possibly invoked from within a scientific workflows tool. Service reconciliation may use the AutoMed heterogeneous data integration system as an intermediary service, or may use AutoMed to produce services that mediate between services. We discuss the application of our approach for the reconciliation of services in an example bioinformatics workflow. The main contribution of this research is an architecture for the scalable reconciliation of bioinformatics services

Utilização de substratos simples e substratos mistos por Debaryomyces hansenii: uma abordagem fisiológica e bioquímica

Poster e resumo apresentado no X Congresso Nacional de Biotecnologia, BIOTEC 2003, Lisboa, 200

EGFR Glycosylation in Cancer

Cancer is a leading cause of mortality around the world. Head and neck (HNC) and esophageal cancers (EC) are the sixth and eighth most common cancers worldwide, respectively. A common feature in both diseases is the overexpression of epidermal growth factor receptor (EGFR). EGFR a transmembrane glycoprotein and cell-surface receptor is mainly involved in regulation of cell proliferation, survival, differentiation and its deregulation is associated with cancer. EGFR is highly glycosylated, and it is known that aberrant glycosylation interferes with its functions. Several EGFR-targeted therapies have been already developed, though showing some undesirable secondary effects, such as skin toxicity, as normal keratinocytes also express EGFR. Identification of a specific target for tumor cells would be essential to minimize those effects. Since EGFR is glycosylated and aberrant glycosylation is a common feature in cancer, identifying a distinct EGFR glycosylation pattern between cancer and normal cells is a promising approach to improve specificity of targeted therapies. In this work, primary human keratinocytes, cell lines and patient-derived tissues representative of HNC and EC were used to implement an optimized strategy to obtain EGFR enriched protein fractions from which EGFR glycosylation pattern could be defined. This thesis reports the results attained for the optimization steps on the different methodologies explored for protein extraction both from cells and tissues, EGFR detection and quantification, EGFR enrichment approaches and glycoprofiling methods (lectin ELISA and LC-MS analysis). Although promising strategies are proposed and validated to extract EGFR from different biological sources in adequate conditions for further mass spectrometry (MS) analysis, some difficulties were found in glycans detection by MS. Further work needs to be done to surpass this method sensitivity problem, as EGFR amounts recovered from enrichment protocols are extremely low, adding to the fact that when dealing with patient-derived samples, specimens are limited in size and availability

Growth Mindset and the Makerspace Educational Environment

This action research project has studied the impact of implementing strategies in a Makerspace on increasing grit in students. The study has been conducted in two fifth grade classes in a mid-western suburban elementary school. During the study, students completed four different Makerspace design challenge activities. Data was collected from Angela Duckworth’s grit survey to establish a baseline of student grit. Documentation of strategies used was collected through an inventory students completed after each design challenge. The data was used to identify changes in grit scores from students’ initial survey results to their final survey results. Student’s survey results were then compared to the total number of strategies used throughout the design challenges. The research data indicated that there was no correlation between the total number of strategies used and an increase in individual grit score. Identifying a tool, or resources to teach and foster grit in students may be increasingly important as grit has been identified as one of the most reliable factors in determining one\u27s success and capacity for academic, professional, or personal success. Keywords: Makerspace, Makerspaces, growth mindset, grit, maker mindset

Shovel Test Pit Paperwork of Transect 3(2) From Lighthouse (8BR234)

This document contains the field notes taken during phase 1 survey for the Lighthouse (8BR234) shovel test pits

Shovel Test Pit Paperwork of Transect 5 From Lighthouse (8BR234)

This document contains the field notes taken during phase 1 survey for the Lighthouse (8BR234) shovel test pits

Shovel Test Pit Paperwork of Transect 6 From Lighthouse (8BR234)

This document contains the field notes taken during phase 1 survey for the Lighthouse (8BR234) shovel test pits

Shovel Test Pit Paperwork of Transect 8 From Lighthouse (8BR234)

This document contains the field notes taken during phase 1 survey for the Lighthouse (8BR234) shovel test pits

Log of All Materials Recovered From Lighthouse (8BR234) (1)

This document is a catalog of artifacts recovered during both phase 1 and phase 2 archaeological excavation. It is a scan of original paper documents generated in the field

Streptococcus Pneumoniae Intracranial Abscess and Post-Infectious Vasculitis

Intracranial abscesses are rare complications of Streptococcus pneumoniae infections, and to our knowledge, there have been no case reports of post-infectious vasculitis developing in such patients. Here we describe the case of a 48-year-old post-splenectomy male who developed post-infectious vasculitis following S. pneumoniae otitis media complicated by mastoiditis, osteomyelitis, meningitis, and intracranial abscess. Clinicians ought to be aware of the possible adverse outcomes of invasive S. pneumoniae and the limitations of current treatment options