早期循证护理对预防重症急性胰腺炎相关性肺损伤的临床 价值研究

Objective: To investigate the severe acute pancreatitis in patient with early application of evidence-based care,where to improve breathing,reduce mortality and shorten the time of ICU. Methods: 80 patients diagnosed with SAP were randomly divided into two groups,A group(control group) 40 cases,B group(evidence-based nursing group) 40 cases,two groups of baseline have no significant difference(P> 0.05).Results: The treatment of respiratory support,B group significantly reduce the rate of endotracheal intubation and invasive ventilation time(P < 0.05). Conclusion: Early evidence-based nursing,significantly reduced the rate of endotracheal intubation patients with SAP,shorten ICU stay time,but did not reduce mortality. 目的  探讨对于重症急性胰腺炎(severe acute pancreatitis，SAP)患者早期应用循证护理，是否改善呼吸情况、降低病死率及缩短入住ICU时间等临床价值。方法  将本院ICU内诊断为SAP患者80例随机分为两组，A组（常规护理对照组）40例，应用常规护理方案；B组（循证护理实验组）40例，给予循证护理方案。两组患者基线水平差异无统计学意义（P > 0.05）。结果  接受呼吸支持治疗方案，B组接受循证护理方案后可显著降低气管插管率及有创通气时间（P < 0.05）；病死率方面，B组在数值上低于A组，但差异无统计学意义，但缩短降低ICU住院时间，B组有明显优势（P < 0.05）。结论  早期循证护理措施，明显降低SAP患者气管插管率，缩短ICU停留时间，但并未降低病死率

Role of the porous structure of the bioceramic scaffolds in bone tissue engineering

The porous structure of biomaterials plays a critical role in improving the efficiency of biomaterials in tissue engineering. Here we fabricate successfully porous bioceramics with accurately controlled pore parameters, and investigate the effect of pore parameters on the mechanical property, the cell seeding proliferation and the vascularization of the scaffolds. This study shows that the porosity play an important role on the mechanical property of the scaffolds, which is affected not only by the macropores size, but also by the interconnections of the scaffolds. Larger pores are beneficial for cell growth in scaffolds. In contrast, the interconnections do not affect cell growth much. The interconnections appear to limit the number of blood vessels penatrating through adjacent pores, and both the pores size and interconnections can determine the size of blood vessels. The results may be referenced on the selective design of porous structure of biomaterials to meet the specificity of biological application

Subcutaneous pedicled propeller flap technique for microscopic reconstruction of eyelid defects

AIM: To describe the subcutaneous pedicled propeller flap technique for the microscopic reconstruction of eyelid defects and evaluate its outcomes. METHODS: The clinical data of 23 patients (23 eyes) who underwent microscopic reconstruction of eyelid defects with the subcutaneous pedicled propeller flap technique were retrospectively analyzed. All patients underwent eyelid tumor resection and one-stage microscopic reconstruction with the subcutaneous pedicled propeller flap for anterior- or posterior-layer eyelid defects. The survival rate of the propeller flap, eyelid function and appearance, tumor recurrence rate, and patient satisfaction were evaluated after the surgery. RESULTS: The patients consisted of 12 men and 11 women, aged 31–82y (mean, 58.9y). The longest follow-up time was 5y, and the shortest was 3mo. All the propeller flaps survived well. There was no significant difference in color and luster between the flap and adjacent tissues, and there was no dog ear phenomenon. No obvious scarring was observed. There were no obvious abnormalities of eyelid morphology or function, and no adverse complications such as exposure keratitis, entropion, ectropion, ptosis, and eyelid retraction. No tumor recurrence was found at the time of the last follow-up. All patients were satisfied with the surgical results. CONCLUSION: The subcutaneous pedicled propeller flap technique for the microscopic reconstruction of eyelid defects has satisfactory outcomes in terms of eyelid function and esthetics, and merits clinical application

Case report of a retroperitoneal cyst

This paper highlights retroperitoneal cysts, its clinical diagnosis, and treatment from a retrospective analysis of clinical data of a 49-year-old female patient diagnosed with retroperitoneal cyst treated in our department. The patient was admitted after an abdominal mass that was discovered on the lower left quadrant for more than six months, showed clinical manifestations. B-mode ultrasound and computed tomography (CT) examinations diagnosed it as a retroperitoneal cyst. A laparotomy was performed for a complete excision of the cyst. Intestinal cysts are rarely reported but retroperitoneal cysts are even more so. Its clinical symptoms are atypical, and therefore easily misdiagnosed before surgery. The best treatment is surgical excision of the cyst, whereby postoperative pathological examination can confirm the diagnosis

Placental expression of AChE, α7nAChR and NF-κB in patients with preeclampsia

Objectives: This study aimed to investigate placental expression of AChE, α7nAChR and NF-κB in patients with preeclampsia and discuss about its clinical significance. Material and methods: mRNA expression levels of acetylcholine (AChE), alpha-7 nicotinic acetylcholine receptor (α7nAChR) and nuclear factor-kB (NF-κB) in placenta were detected by qRT-PCR, and protein levels were determined by immunohis­tological analysis and Western Blot in 35 women with preeclampsia (including 20 cases of mild preeclampsia and 15 cases of severe preeclampsia) and 30 cases in control group, respectively. Results: The expression of AChE mRNA and protein in placenta increased significantly in patients with preeclampsia compared with the control group (p < 0.01). It was lower in patients with severe preeclampsia than in patients with mild preeclampsia (p < 0.05). The expression of α7nAChR mRNA and protein in placenta decreased significantly in patients with preeclampsia compared with the control group (p < 0.01). However, the expression of α7nAChR mRNA and protein in patients with severe preeclampsia was higher than that in patients with mild preeclampsia, without significant difference(p > 0.05). The expression of NF-κB protein in placenta decreased significantly in patients with preeclampsia compared with the control group(p < 0.01). It was higher in patients with severe preeclampsia than in patients with mild preeclampsia (p < 0.05), but there was no significant difference between preeclampsia group and control group in the expression of NF-κB mRNA in placenta (p > 0.05). The results of Western blotting assay were consistent with those of immunohistochemistry. Conclusions: Abnormal expression of AChE, α7nAChR and NF-κB in placenta may be associated with preeclampsia. Cho­linergic anti-inflammatory pathway may play an important role in the pathogenesis of preeclampsia

Virus efficacy of recombined Autographa californica M nucleopolyhedrovirus (AcMNPV) on tea pest Ectropis obliqua

Ectropis obliqua is a major tea pest and chitin synthase (CHS) plays a key role in the pest growth and development. A 192 bp conserved domain from E. obliqua CHS gene was cloned and it was used to construct recombined Autographa californica M nucleopolyhedrovirus (AcMNPV) with double-stranded RNA interference (dsRNAi) method. The recombined AcMNPV virus could propagate in host cells sf9. Injection test showed that the virus efficacy of the recombined AcMNPV on E. obliqua larvae was significantly enhanced. It is considered that the CHS dsRNAi mediated by the nuclear polyhedrosis virus will be interesting for development of alternative bio-pesticide to control the tea pest E. obliqua.Keywords: Chitin synthase, baculovirus, double-stranded RNA interference, Ectropis obliquaAfrican Journal of Biotechnology Vol. 9(33), pp. 5412-5418, 16 August, 201

Shaping symmetric Airy beam through binary amplitude modulation for ultralong needle focus

Needle-like electromagnetic field has various advantages for the applications in high-resolution imaging, Raman spectroscopy, as well as long-distance optical transportation. The realization of such field often requires high numerical aperture (NA) objective lens and the transmission masks. We demonstrate an ultralong needle-like focus in the optical range produced with an ordinary lens. This is achieved by focusing a symmetric Airy beam (SAB) generated via binary spectral modulation with a digital micromirror device. Such amplitude modulation technique is able to shape traditional Airy beams, SABs, as well as the dynamic transition modes between the one-dimensional and two-dimensional (2D) symmetric Airy modes. The created 2D SAB was characterized through measurement of the propagating fields with one of the four main lobes blocked by an opaque mask. The 2D SAB was verified to exhibit self-healing property against propagation with the obstructed major lobe reconstructed after a certain distance. We further produced an elongated focal line by concentrating the SAB via lenses with different NAs and achieved an ultralong longitudinal needle focus. The produced long needle focus will be applied in optical, chemical, and biological sciences.Fil: Zhao Xiang, Fang. University of Science and Technology of China; ChinaFil: Yu Xuan, Ren. Shanghai Institutes for Biological Sciences; ChinaFil: Gong, Lei. University of Science and Technology of China; ChinaFil: Vaveliuk, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Investigaciones Opticas (i); Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; ArgentinaFil: Chen, Yue. University of Science and Technology of China; ChinaFil: Rong De, Lu. University of Science and Technology of China; Chin

Genome-wide identification, phylogenetic and expression analysis of the heat shock transcription factor family in bread wheat (Triticum aestivum L.)

BackgroundEnvironmental toxicity from non-essential heavy metals such as cadmium (Cd), which is released from human activities and other environmental causes, is rapidly increasing. Wheat can accumulate high levels of Cd in edible tissues, which poses a major hazard to human health. It has been reported that heat shock transcription factor A 4a (HsfA4a) of wheat and rice conferred Cd tolerance by upregulating metallothionein gene expression. However, genome-wide identification, classification, and comparative analysis of the Hsf family in wheat is lacking. Further, because of the promising role of Hsf genes in Cd tolerance, there is need for an understanding of the expression of this family and their functions on wheat under Cd stress. Therefore, here we identify the wheat TaHsf family and to begin to understand the molecular mechanisms mediated by the Hsf family under Cd stress.ResultsWe first identified 78 putative Hsf homologs using the latest available wheat genome information, of which 38 belonged to class A, 16 to class B and 24 to class C subfamily. Then, we determined chromosome localizations, gene structures, conserved protein motifs, and phylogenetic relationships of these TaHsfs. Using RNA sequencing data over the course of development, we surveyed expression profiles of these TaHsfs during development and under different abiotic stresses to characterise the regulatory network of this family. Finally, we selected 13 TaHsf genes for expression level verification under Cd stress using qRT-PCR.ConclusionsTo our knowledge, this is the first report of the genome organization, evolutionary features and expression profiles of the wheat Hsf gene family. This work therefore lays the foundation for targeted functional analysis of wheat Hsf genes, and contributes to a better understanding of the roles and regulatory mechanism of wheat Hsfs under Cd stress