EVALUATION OF HEAVY METALS AROUND THE MINING OF DECORATIVE STONE ORE IN SUSONG COUNTY LIAOHE RIVER

In order to study the pollution of heavy metals around Liaohe Fender stone mine in Susong County, the soils at six points and the sediment at four points were selected. The effects of heavy metals Cu, Zn, Pb, Cd, Cr , Ni ,Hg and As were measured, the single factor index and the Nemero index method were used to evaluate the heavy metal elements in soil and sediment. The results showed that the values of heavy metal elements in the soil and sediment were less than 1 and the Pintegrated values were less than 0.85,the mine area was not polluted by heavy metals and belonged to the clean area within the grade Ⅰ

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

The Synthesis of a Coumarin Carbohydrazide Dinuclear Copper Complex Based Fluorescence Probe and Its Detection of Thiols.

Small-molecule thiols, such as cysteine (CYS) and glutathione (GSH), are essential for maintaining the cellular redox environment and play important roles in regulating various cellular physiological functions. A fluorescence probe (compound 1-Cu2+) for thiols based on coumarin carbohydrazide dinuclear copper complex was developed. Compound 1 was synthesized from the reaction of 7-(diethylamino)-2-oxo-2H-chromene-3-carbohydrazide with 4-tert-butyl-2,6- diformylphenol. Accordingly, the copper complex (compound 1-Cu2+) was prepared by mixing compound 1 with 2 equivalents copper ions. Compound 1 had strong fluorescence while compound 1-Cu2+ hardly possessed fluorescence owing to the quenching nature of paramagnetism Cu2+ to the fluorescence molecule excited state. However, the fluorescence intensity of compound 1-Cu2+ was increased dramatically after the addition of thiol-containing amino acids, but not the other non-sulfhydryl amino acids. UV-vis absorption and fluorescence spectra indicated that compound 1-Cu2+ had good selectivity and sensitivity for thiols such as glutathione in CH3CN:H2O (3:2, v/v) PBS solution. The fluorescence imaging experiments implied that compound 1-Cu2+ has potential application in thiol-containing amino acids detection in living cells

Fluorescence intensity (λ_em = 483 nm) of compound 1-Cu²⁺ to various amino acids: the first bars represent the fluorescence intensity upon addition of 4 equivalents of various amino acids; the second bars represent the fluorescence intensity after subsequent addition of 2 equivalents of GSH to the non-sulfhydryl amino acids solution, respectively.

<p>Fluorescence intensity (λ_em = 483 nm) of compound 1-Cu²⁺ to various amino acids: the first bars represent the fluorescence intensity upon addition of 4 equivalents of various amino acids; the second bars represent the fluorescence intensity after subsequent addition of 2 equivalents of GSH to the non-sulfhydryl amino acids solution, respectively.</p

Synthesis procedures of the thiols probe (compound 1-Cu²⁺).

<p>Synthesis procedures of the thiols probe (compound 1-Cu²⁺).</p

UV-vis titration of compound 1 (10 μmol/L) in CH₃CN:H₂O (3:2, v/v) PBS solution upon addition of Cu²⁺.

<p>Inset: UV-vis titration profile of compounds <b>1</b> upon addition of Cu²⁺, the absorption was recorded at 480 nm.</p

Fluorescence emission spectra of compound 1 (10 μmol/L) in CH₃CN:H₂O (3:2, v/v) PBS solution with successive addition of Cu²⁺.

<p>Insert: fluorescence titration profile at 483 nm upon the addition of Cu²⁺ (excited at 445 nm).</p