Public sector reform and the state of performance management in Portugal: is there a gap between performance measurement and its use?

Purpose – This paper analyses the state of performance management in the Portuguese public sector as part of the efforts towards public administration reform. Design/methodology/approach – Theoretically, we took Bouckaert and Halligan’s (2008, pp. 35-39) approach into consideration to analyse the adoption of performance management practices. This approach was supplemented by an adaptation of Pollitt and Bouckaert’s (2011, p. 33) framework to analyse the context for administrative reforms. The used data analysis techniques include documentary analysis (namely legislation and evaluation reports of reform efforts), secondary data analysis and a survey conducted with 296 Portuguese top public managers. Findings – Findings show that Portuguese public sector organizations adopted several tools to measure performance over the years, but failed to incorporate performance information into their management practices or to properly use it for either internal or external purposes. Concerning the ideal types proposed by Bouckaert and Halligan (2008, p. 36), Portugal is considered to fit the ‘Performance Administration’ ideal type, even though it is moving closer to the ‘Managements of Performance’ ideal type. Originality – This is one of the first comprehensive studies on the state of performance management in Portugal framed within the broader context of public sector reforms. The findings will be of interest both to scholars that study public administration reforms and performance management and to Portuguese policymakers and public managers who are interested in understanding and improving the way performance information is measured, incorporated and used in that sector.publishe

Detection and discrimination of microorganisms using Locked Nucleic Acid - Fluorescence In Situ Hybridization (LNA-FISH)

The impact of multispecies biofilms on catheter-associated urinary tract infections outcome is still unclear due to the lack of adequate methodologies to discriminate the populations in situ. Employing fluorescence in situ hybridization (FISH) to discriminate the populations in a biofilm, can contribute to the understanding of microorganisms interactions in this structures. Consequently, this information might help to develop efficient strategies to prevent this disease. This work presents the first study that apply the FISH methodology using a set of LNA and/2’-O-Methyl RNA oligonucleotide probes, for the in situ detection of microorganisms in biofilms formed under conditions similar to the catheter-associated urinary tract infections

Interaction between atypical microorganisms and E. coli in catheter-associated urinary tract biofilms

Most biofilms involved in catheter-associated urinary tract infections (CAUTIs) are polymicrobial, with disease causing (eg Escherichia coli) and atypical microorganisms (eg Delftia tsuruhatensis) frequently inhabiting the same catheter. Nevertheless, there is a lack of knowledge about the role of atypical microorganisms. Here, single and dual-species biofilms consisting of E. coli and atypical bacteria (D. tsuruhatensis and Achromobacter xylosoxidans), were evaluated. All species were good biofilm producers (Log 5.84–7.25 CFU cm−2 at 192 h) in artificial urine. The ability of atypical species to form a biofilm appeared to be hampered by the presence of E. coli. Additionally, when E. coli was added to a pre-formed biofilm of the atypical species, it seemed to take advantage of the first colonizers to accelerate adhesion, even when added at lower concentrations. The results suggest a greater ability of E. coli to form biofilms in conditions mimicking the CAUTIs, whatever the pre-existing microbiota and the inoculum concentration.This work was supported by the Portuguese Science Foundation (FCT), DNA mimics Research Project [Ref. PIC/IC/82815/2007] from the FCT and MCTES; PhD Fellowship [SFRH/BD/82663/2011]; and Postdoctoral Fellowship [SFRH/BPD/74480/2010]. The authors would like to thank to M. Fenice M and A. Steinbuchel for kindly providing the Delftia tsuruhatensis BM90 and Achromobacter xylosoxidans B3 species, respectively

Impact of Delftia tsuruhatensis and Achromobacter xylosoxidans on Escherichia coli dual-species biofilms treated with antibiotic agents

Recently it was demonstrated that for urinary tract infections species with a lower or unproven pathogenic potential, such as Delftia tsuruhatensis and Achromobacter xylosoxidans, might interact with conventional pathogenic agents such as Escherichia coli. Here, single- and dual-species biofilms of these microorganisms were characterized in terms of microbial composition over time, the average fitness of E. coli, the spatial organization and the biofilm antimicrobial profile. The results revealed a positive impact of these species on the fitness of E. coli and a greater tolerance to the antibiotic agents. In dual-species biofilms exposed to antibiotics, E. coli was able to dominate the microbial consortia in spite of being the most sensitive strain. This is the first study demonstrating the protective effect of less common species over E. coli under adverse conditions imposed by the use of antibiotic agents.This work was financially supported by the FCT/MEC with national funds and when applicable co-funded by FEDER in the scope of the P2020 Partnership Agreement [Project UID/ EQU/00511/2013-LEPABE]; FEDER funds through the Operational Programme for Competitiveness Factors – COMPETE, O Novo Norte–North Portugal Regional Operational Programme – ON2 and National Funds through Foundation for Science and Technology – FCT [Project NORTE-07-0124- FEDER-000025–RL2_ Environment&Health and Project ‘DNAmimics’ PIC/IC/82815/2007]; PhD fellowship [SFRH/ BD/82663/2011]; and postdoctoral fellowship [SFRH/ BPD/74480/2010]

An in vitro dynamic model of catheter-associated urinary tract infections to investigate the role of uncommon bacteria on the Escherichia coli microbial consortium

About 9% of nosocomial infections are attributed to catheter-associated urinary tract infections (CAUTIs). Uncommon bacteria (Delftia tusurhatensis) have been isolated in CAUTIs in combination with wellestablished pathogenic bacteria such as E. coli. Nonetheless, the reason why E. coli coexists with other bacteria instead of outcompeting and completely eliminating them are unknown. As such, a flow cell reactor simulating the hydrodynamic conditions found in CAUTIs (shear rate of 15 s-1) was used to characterize the microbial physiology of E. coli and D. tsuruhatensis individually and in consortium, in terms of growth kinetics and substrate uptake. Single-species biofilms showed that up to 48 h the CFU counts significantly increased for both species (p<0.05). After 48 h, both species stabilized with similar CFU values reaching log 6.24 CFU.cm2 for E. coli and log 6.31 CFU.cm2 for D. tsuruhatensis (p>0.05). The assessment of spatial distribution of dual-species biofilms by LNA/2´OMe-FISH revealed that E. coli and D. tsuruhatensis coexist and tend to co-aggregate over time, which implies that bacteria are able to cooperate synergistically. Substrate uptake measurements revealed that in artificial urine medium the bacteria metabolized lactic acid, uric acid (E. coli and D. tsuruhatensis) and citric acid (D. tsuruhatensis). In the consortium, D. tsuruhatensis consumed citric acid more rapidly, presumably leaving more uric acid available in the medium to be used by E. coli. In conclusion, metabolic cooperation between E. coli and uncommon species seems to occur when these species share the same environment, leading to the formation of a stable microbial community

Aptasensor for the detection of Moraxella catarrhalis adhesin UspA2

Innovative point-of-care (PoC) diagnostic platforms are desirable to surpass the deficiencies of conventional laboratory diagnostic methods for bacterial infections and to tackle the growing antimicrobial resistance crisis. In this study, a workflow was implemented, comprising the identification of new aptamers with high affinity for the ubiquitous surface protein A2 (UspA2) of the bacterial pathogen Moraxella catarrhalis and the development of an electrochemical biosensor functionalized with the best-performing aptamer as a bioreceptor to detect UspA2. After cell-systematic evolution of ligands by exponential enrichment (cell-SELEX) was performed, next-generation sequencing was used to sequence the final aptamer pool. The most frequent aptamer sequences were further evaluated using bioinformatic tools. The two most promising aptamer candidates, Apt1 and Apt1_RC (Apt1 reverse complement), had Kd values of 214.4 and 3.4 nM, respectively. Finally, a simple and label-free electrochemical biosensor was functionalized with Apt1_RC. The aptasensor surface modifications were confirmed by impedance spectroscopy and cyclic voltammetry. The ability to detect UspA2 was evaluated by square wave voltammetry, exhibiting a linear detection range of 4.0 × 104–7.0 × 107 CFU mL−1, a square correlation coefficient superior to 0.99 and a limit of detection of 4.0 × 104 CFU mL−1 at pH 5.0. The workflow described has the potential to be part of a sensitive PoC diagnostic platform to detect and quantify M. catarrhalis from biological samples.The study received financial support from the ViBrANT project, which received funding from the EU Horizon 2020 Research and Innovation Programme under the Marie Sklowdowska Curie, grant agreement no. 765042. In addition, the authors acknowledge the financial support from Fundação para a Ciência e Tecnologia (FCT) under the scope of the strategic funding of UID/BIO/04469/2020 unit and of LABBELS—Associate Laboratory in Biotechnology, Bioengineering and Microelectromechanical Systems, LA/P/0029/2020. D.L. and A.S. received additional funding from the Research Council of Norway (grant 294605, Center for Digital Life). L.D.R.M. acknowledges funding from the FCT through the Scientific Employment Stimulus Program (2021.00221.CEECIND).info:eu-repo/semantics/publishedVersio

Fighting methicillin-resistant staphylococcus aureus with targeted nanoparticles

Antimicrobial resistance (AMR) is considered one of the greatest threats to global health. Methicillin-resistant Staphylococcus aureus (MRSA) remains at the core of this threat, accounting for about 90% of S. aureus infections widespread in the community and hospital settings. In recent years, the use of nanoparticles (NPs) has emerged as a promising strategy to treat MRSA infections. NPs can act directly as antibacterial agents via antibiotic-independent activity and/or serve as drug delivery systems (DDSs), releasing loaded antibiotics. Nonetheless, directing NPs to the infection site is fundamental for effective MRSA treatment so that highly concentrated therapeutic agents are delivered to the infection site while directly reducing the toxicity to healthy human cells. This leads to decreased AMR emergence and less disturbance of the individual’s healthy microbiota. Hence, this review compiles and discusses the scientific evidence related to targeted NPs developed for MRSA treatment.info:eu-repo/semantics/publishedVersio