不同年龄和性别精神分裂症患者脑白质扩散张量成像的临床研究*

Objective: Diffusion tensor imaging (DTI) was used to analyze the changes of white matter fiber FA in patients with schizophrenia of different ages and genders, and to explore the reference of clinical imaging. Methods: Retrospective analysis of the clinical diagnosis of schizophrenia patients and healthy subjects in all 50 cases, were given routine examination of brain MRI parallel diffusion tensor imaging, comparison of different age and gender in different parts of the brain white matter changes of FA value. Results: (1) the FA values of white matter in different age groups were different between the patients and the normal group (P < 0.05). The normal group right superior frontal gyrus, left parietal lobe and left anterior cingulate gyrus of the cerebral white matter fiber FA value increased gradually before the age of 30, at the age of 30 and reached the peak gradually decreased after 30 years old. The left frontal gyrus and corpus callosum in patients with group pressure (after) of cerebral white matter fiber FA value increased gradually before the age of 30, at the age of 30 and reached the peak gradually decreased after 30 years old. (2) the FA values of white matter in different parts of male and female patients were different (P < 0.05). The white matter fiber FA in the left anterior capsule of the normal group was higher in males than in females. The FA value of bilateral occipital white matter in male patients was lower than that in female; the FA values of the central white matter in the left and right sides of the brain stem were higher in males than in females. Conclusion: the changes of FA value in the white matter of some parts of the brain in the normal group and the patient group are influenced by age and sex.  目的  运用扩散张量成像分析不同年龄和性别精神分裂症患者脑白质纤维FA值的变化，探讨临床影像学参考依据。方法  回顾性分析经临床确诊的精神分裂症患者和健康者各50例，均作颅脑MRI常规检查并行扩散张量成像，比较不同年龄和性别各部位脑白质FA值变化特点。结果  （1）患者组和正常组不同年龄各部位脑白质FA值有差异（P＜0.05）。正常组中右额上回、左顶叶及左扣带回前部脑白质纤维FA值在30岁前逐渐增高，30岁达高峰，在30岁后逐渐减少。患者组中左额上回及胼胝体压部（后）脑白质纤维FA值在30岁前逐渐增高，30岁达高峰，在30岁后逐渐减少。（2）患者组和正常组男女各部位脑白质FA值有差异（P＜0.05）。发现正常组中左侧内囊前肢脑白质纤维FA值男性较女性高。患者组中双侧枕叶脑白质纤维FA值男性较女性低；脑干左右侧中心脑白质FA值男性较女性高。结论  正常组和患者组大脑某些部位脑白质纤维FA值量的变化受年龄、性别的影响

A combined method for gas-bearing layer identification in a complex sandstone reservoir

Langgu Depression is a mature oil and gas exploration area with complicated lithological and physical properties. The varying formation fluid, low-resistivity hydrocarbon-bearing reservoirs, and non-uniform logging series greatly increase the difficulty of gas reservoir identification. The Monte Carlo method is employed to simulate the neutron–gamma logging responses to gas saturation and the influential factors. According to the result, a new gas identification chart eliminating the influence of porosity and formation water salinity is proposed to identify gas reservoirs in the old wells. At the same time, a fluid factor extracted from array acoustic logging and core measurement data is sensitive to the development of gas-bearing layers and useful for the identification of gas reservoirs in the new wells with array acoustic logging. The field examples show that the new combined method greatly improves the ability to identify gas-bearing layers and works well in old well reexamination and new well interpretation

Quality of reporting of systematic reviews published in “evidence-based” Chinese journals

BACKGROUND: The number of systematic reviews (SRs)/meta-analyses (MAs) has increased dramatically in China over the past decades. However, evaluation of quality of reporting of systematic reviews published has not been undertaken. The objective of this study is to evaluate the quality of reporting of SRs/MAs assessing efficacy and/or harms of clinical interventions published in “evidence-based” Chinese journals. METHODS: Web-based database searches were conducted for the Chinese Journal of Evidence-based Medicine, the Journal of Evidence-Based Medicine, the Chinese Journal of Evidence Based Pediatrics, and the Chinese Journal of Evidence-Based Cardiovascular Medicine. SRs/MAs assessing efficacy and/or harms of clinical interventions were included. The cut-off was December 31st 2011. The PRISMA statement was applied to assess the quality of reporting. Each item was assessed as follows: ‘Yes’ for total compliance, scored ‘1’; ‘partial’ for partial compliance, scored ‘0.5’; and ‘No’ for non-compliance, scored ‘0’. The review was considered to have major flaws if it received a total score of ≤15.0, minor flaws if it received a total score of 15.5 to 21.0, and minimal flaws if it received a total score 21.5 to 27.0. Odds ratios were used for binary variables, and the mean difference was used for continuous variables. Analyses were performed using RevMan 5.0 software. RESULTS: Overall, 487 SRs/MAs were identified and assessed. The included reviews had medium quality with minor flaws based on PRISMA total scores (range: 8.5–26.0; mean: 19.6 ± 3.3). The stratified analysis showed that SRs/MAs with more than 3 authors, from a university, hospital + university cooperation, multiple affiliations (≥2), and funding have significantly higher quality of reporting of SRs/MAs; 58% of the included reviews were considered to have minor flaws (total score of 15.6 to 21.0). Only 9.6% of reviews were considered to have major flaws. Specific areas needing improvement in reporting include the abstract, protocol and registration, and characteristics of the search. CONCLUSIONS: The reporting of SRs published in “evidence-based” Chinese journals is poor and needs to be improved in order for reviews to be useful. SR authors should use the PRISMA checklist to ensure complete and accurate accounts of their SRs

A predator-prey interaction between a marine Pseudoalteromonas sp. and Gram-positive bacteria

Predator-prey interactions play important roles in the cycling of marine organic matter. Here we show that a Gram-negative bacterium isolated from marine sediments (Pseudoalteromonas sp. strain CF6-2) can kill Gram-positive bacteria of diverse peptidoglycan (PG) chemotypes by secreting the metalloprotease pseudoalterin. Secretion of the enzyme requires a Type II secretion system. Pseudoalterin binds to the glycan strands of Gram positive bacterial PG and degrades the PG peptide chains, leading to cell death. The released nutrients, including PG-derived D-amino acids, can then be utilized by strain CF6-2 for growth. Pseudoalterin synthesis is induced by PG degradation products such as glycine and glycine-rich oligopeptides. Genes encoding putative pseudoalterin-like proteins are found in many other marine bacteria. This study reveals a new microbial interaction in the ocean

Integrated Analysis of microRNA and mRNA Transcriptome Reveals the Molecular Mechanism of \u3ci\u3eSolanum lycopersicum\u3c/i\u3e Response to \u3ci\u3eBemisia tabaci\u3c/i\u3e and \u3ci\u3eTomato chlorosis virus\u3c/i\u3e

Tomato chlorosis virus (ToCV), is one of the most devastating cultivated tomato viruses, seriously threatened the growth of crops worldwide. As the vector of ToCV, the whitefly Bemisia tabaci Mediterranean (MED) is mainly responsible for the rapid spread of ToCV. The current understanding of tomato plant responses to this virus and B. tabaci is very limited. To understand the molecular mechanism of the interaction between tomato, ToCV and B. tabaci, we adopted a next-generation sequencing approach to decipher miRNAs and mRNAs that are differentially expressed under the infection of B. tabaci and ToCV in tomato plants. Our data revealed that 6199 mRNAs were significantly regulated, and the differentially expressed genes were most significantly associated with the plant-pathogen interaction, the MAPK signaling pathway, the glyoxylate, and the carbon fixation in photosynthetic organisms and photosynthesis related proteins. Concomitantly, 242 differentially expressed miRNAs were detected, including novel putative miRNAs. Sly-miR159, sly-miR9471b-3p, and sly-miR162 were the most expressed miRNAs in each sample compare to control group. Moreover, we compared the similarities and differences of gene expression in tomato plant caused by infection or co-infection of B. tabaci and ToCV. Taken together, the analysis reported in this article lays a solid foundation for further research on the interaction between tomato, ToCV and B. tabaci, and provide evidence for the identification of potential key genes that influences virus transmission in tomato plants

Integrated Analysis of microRNA and mRNA Transcriptome Reveals the Molecular Mechanism of Solanum lycopersicum Response to Bemisia tabaci and Tomato chlorosis virus

Tomato chlorosis virus (ToCV), is one of the most devastating cultivated tomato viruses, seriously threatened the growth of crops worldwide. As the vector of ToCV, the whitefly Bemisia tabaci Mediterranean (MED) is mainly responsible for the rapid spread of ToCV. The current understanding of tomato plant responses to this virus and B. tabaci is very limited. To understand the molecular mechanism of the interaction between tomato, ToCV and B. tabaci, we adopted a next-generation sequencing approach to decipher miRNAs and mRNAs that are differentially expressed under the infection of B. tabaci and ToCV in tomato plants. Our data revealed that 6199 mRNAs were significantly regulated, and the differentially expressed genes were most significantly associated with the plant-pathogen interaction, the MAPK signaling pathway, the glyoxylate, and the carbon fixation in photosynthetic organisms and photosynthesis related proteins. Concomitantly, 242 differentially expressed miRNAs were detected, including novel putative miRNAs. Sly-miR159, sly-miR9471b-3p, and sly-miR162 were the most expressed miRNAs in each sample compare to control group. Moreover, we compared the similarities and differences of gene expression in tomato plant caused by infection or co-infection of B. tabaci and ToCV. Taken together, the analysis reported in this article lays a solid foundation for further research on the interaction between tomato, ToCV and B. tabaci, and provide evidence for the identification of potential key genes that influences virus transmission in tomato plants