224 research outputs found
Non-Immunoglobulin-E mediated gastrointestinal food allergy in children: diagnosis, management and clinical impact
Background: Despite all research published in the last decades, the prevalence of
food allergy in the paediatric population has continued to increase and is nowadays
recognized as a significant public health problem. In the United Kingdom, studies
have found that between 2.2% and 5.5% of the infants in the first year of life present
with proven food allergies, with the prevalence of cowβs milk protein allergy, the most
common form of non-IgE food allergies, ranging between 1.9% and 4.9%. The
diagnosis of non-IgE mediated food allergy is a real challenge due to its delayed
onset, with symptoms occurring hours to days after ingestion of the culprit and
mainly affecting the gastrointestinal tract. Allergy tests have shown limited use in
these patients, and the diagnosis relies heavily on the recognition of symptoms and
an allergy-focused history. The treatment is based on diet elimination, which can
lead to the development of nutritional disorders and negative impact on the childβs
quality of life.
Objectives: The aims of the publications included in this thesis are, first, to
prospectively investigate the required length of elimination diet to achieve symptom
improvement during the diagnostic process; second, to evaluate the knowledge of
primary health care professionals about diagnosis and management of cowβs milk
protein allergy, to compare with parents views and opinions, and to better
understand diagnosis tools, treatment and tolerance in infants diagnosed with nonIgE food allergies; third, to evaluate possible impacts of the elimination diet on
growth and nutrients deficiencies; and fourth, to analyse the potential impact of nonIgE mediated gastrointestinal allergies on the quality of life of the affected children
and their families.
Methods: Children with suspected non-IgE mediated gastrointestinal food allergies
were recruited and followed prospectively at a large tertiary gastroenterology
department in the United Kingdom (UK) from December 2011 to November 2013.
This study was designed to investigate the impact of non-IgE mediated food allergies
on quality of life, as well as to establish symptoms, childrenβs growth and the
characteristics and impact of dietary elimination. The knowledge and journey of
diagnosis of cowβs milk protein allergy among general practitioners were studied via
surveys. Eosinophilic colitis in infants was studied through a systematic review.
II
Results: In non-IgE mediated gastrointestinal food allergies the required time to
achieve symptom improvement following the initiation of the elimination diet was four
weeks in 98% of our patients. All symptoms individually and collectively have
statistically improved after commencing the elimination diet (p <0.001). Regarding
cowβs milk protein allergy, parents and general practitioners have very different
perceptions about the course of the disease and the familiesβ journeys. An extensive
review of the literature found that eosinophilic colitis occurred more frequently in
male infants, younger than six months old. Half were exclusively breastfed at the
time of the diagnosis. Patients on a diagnostic four-week elimination diet showed an
improvement in the weight-for-age and weight-for-height. It was also noted that 60%
of the cohort had low vitamin D intake and a higher number of patients had a low
intake of copper, zinc, and selenium. Non-IgE mediated food allergies have a
negative impact on the quality of life of the affected children and their families.
Conclusions: The βDiscovering the effect of non-IgE mediated allergies on children
and their familiesβ is a landmark study in the paediatric food allergy field. This welldesigned study investigated and elucidated some important characteristics and
clinical features of this group of patients. Its impact is reflected in the number of
publications derived from the results of this cohort, a large number of citations and
the impact in clinical practice worldwide as much more is now known about this
condition. Studies investigating preventative measures for the development of food
allergies in childhood are also essential in the future
Sorption of microorganisms by wide-porous agarose cryogels containing grafted aliphatic chains of different length
The possibility of fractionation of heterogeneous bacterial populations using wide-porous agarose cryogels containing grafted aliphatic groups with the chain lengths of 4, 7, and 12 carbon atoms was demonstrated for the first time. The maximum sorption of vegetative cells of gram-positive bacteria (60%) was shown for the polymeric carrier with the chain length of 4 carbon atoms, while the hypometabolic cells appearing in the population after prolonged (60-day) cultivation were trapped by wide-porous affinity sorbents with C7- and C12- aliphatic groups much better than vegetative cells. Β© Pleiades Publishing, Ltd 2009
Digital receivers for low-frequency radio telescopes UTR-2, URAN, GURT
This paper describes digital radio astronomical receivers used for decameter
and meter wavelength observations. This paper describes digital radio
astronomical receivers used for decameter and meter wavelength observations.
Since 1998, digital receivers performing on-the-fly dynamic spectrum
calculations or waveform data recording without data loss have been used at the
UTR-2 radio telescope, the URAN VLBI system, and the GURT new generation radio
telescope. Here we detail these receivers developed for operation in the strong
interference environment that prevails in the decameter wavelength range. Data
collected with these receivers allowed us to discover numerous radio
astronomical objects and phenomena at low frequencies, a summary of which is
also presented.Comment: 24 pages, 15 figure
Study protocol of a multicentre, randomised, controlled trial evaluating the effectiveness of probiotic and peanut oral immunotherapy (PPOIT) in inducing desensitisation or tolerance in children with peanut allergy compared with oral immunotherapy (OIT) alone and with placebo (the PPOIT-003 study)
Introduction: Peanut allergy is the the most common cause of life-threatening food-induced anaphylaxis. There is currently no effective long-term treatment. There is a pressing need for definitive treatments that improve the quality of life and prevent fatalities. Allergen oral immunotherapy (OIT) is a promising approach, which is effective at inducing desensitisation; however, OIT has a limited ability to induce sustained unresponsiveness (SU). We have previously shown that a novel treatment comprising a combination of the probiotic Lactobacillus rhamnosus CGMCC 1.3724 with peanut OIT (Probiotic Peanut Oral ImmunoTherapy (PPOIT)) is highly effective at inducing SU, with benefit persisting to 4 years after treatment cessation in the majority of initial treatment responders. Here we describe the protocol for a Phase IIb multicentre, double-blind, randomised, controlled trial (PPOIT-003) with dual primary objectives to evaluate the effectiveness of PPOIT at inducing SU (assessed at 8 weeks after treatment cessation) compared with placebo treatment and peanut OIT alone, in children with peanut allergy. Methods and analysis: 200 children 1 to 10 years of age with current peanut allergy confirmed by failed double-blind placebo-controlled food challenge (DBPCFC) at study screening will be recruited from three tertiary paediatric hospitals in Australia. There are three intervention armsβPPOIT, peanut OIT alone or placebo. Interventions are administered once daily for 18 months. The dual primary outcomes are: (1) the proportion of children who attain 8-week SU in the PPOIT group versus placebo group and (2) the proportion of children who attain 8-week SU in the PPOIT group versus OIT group. Ethics and dissemination: This study has been approved by the Human Research Ethics Committees at the Royal Childrenβs Hospital (HREC 35246) and the Child and Adolescent Health Service (RGS 2543). Results will be published in peer-reviewed journals and disseminated via presentations at international conferences.Adriana Chebar Lozinsky, Paxton Loke, Francesca Orsini, Michael OβSullivan, Susan L. Prescott, Michael S Gold, Patrick Quinn, Audrey DunnGalvin, Mimi LK Tang, on behalf of the PPOIT study tea
ΠΡΠΈΠΎΠ³Π΅Π»ΠΈ ΠΏΠΎΠ»ΠΈΠ²ΠΈΠ½ΠΈΠ»ΠΎΠ²ΠΎΠ³ΠΎ ΡΠΏΠΈΡΡΠ°, ΡΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½Π½ΡΠ΅ ΠΈΠ· ΡΠ°ΡΡΠ²ΠΎΡΠΎΠ² ΠΏΠΎΠ»ΠΈΠΌΠ΅ΡΠ° Π² Π΄ΠΈΠΌΠ΅ΡΠΈΠ»ΡΡΠ»ΡΡΠΎΠΊΡΠΈΠ΄Π΅ Ρ Π΄ΠΎΠ±Π°Π²ΠΊΠ°ΠΌΠΈ ΡΠ΅ΡΡΠ°ΠΌΠ΅ΡΠΎΠΊΡΠΈΡΠΈΠ»Π°Π½Π°
Organicβinorganic hybrid cryogels of poly(vinyl alcohol) (PVA) containing silica inclusions (SiO2) were obtained and studied. Such inclusions were formed in the course of hydrolytic polycondensation (sol-gel process) of tetramethoxysilane (TMOS) introduced into concentrated polymer solutions in dimethyl sulfoxide (DMSO). PVA concentration in these solutions was 60, 80 or 100 g/L; while the concentration of TMOS was varied in a range of 0.15β0.61 mol/L. The polymer solutions were subjected to the cryogenic treatment at temperatures by 40 Β°Π‘ lower than DMSO crystallization temperature (+18.4Β°C). The frozen samples were thawed out at a heating rate of 0.03Β°/min. It is shown that moderate freezing at β21.6 Β°C, then frozen storage and subsequent thawing of the initial reaction mixture PVA/DMSO/TMOS/acid catalyst resulted in the formation of strong macroporous PVA/SiO2 cryogels. Such cryogel materials are hybrid systems, because the gel-forming polymer and the silica containing moieties belong to the content of the gel phase. The basis of this intermolecular interaction is hydrogen bonding between OH groups of the adjacent chains. This leads to the formation of modified microcrystallinity zones that perform as the nodes of the three-dimensional network of cryogels. The effects of significant increase in their rigidity and heat-resistance with increasing PVA and TMOS concentration in the initial feed were also observed. It was shown that the success of the synthesis of transparent elastic and heat-resistant PVA/SiO2 cryogels depends on the choice of the optimal ratio between the precursors and the combined effect of the liquid (methanol and water) and silicon-containing components on the formation of multiple hydrogen bonds and microcrystallites.ΠΠΎΠ»ΡΡΠ΅Π½Ρ ΠΈ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½Ρ ΠΎΡΠ³Π°Π½ΠΎ-Π½Π΅ΠΎΡΠ³Π°Π½ΠΈΡΠ΅ΡΠΊΠΈΠ΅ Π³ΠΈΠ±ΡΠΈΠ΄Π½ΡΠ΅ ΠΊΡΠΈΠΎΠ³Π΅Π»ΠΈ ΠΏΠΎΠ»ΠΈΠ²ΠΈΠ½ΠΈΠ»ΠΎΠ²ΠΎΠ³ΠΎ ΡΠΏΠΈΡΡΠ° (ΠΠΠ‘), ΡΠΎΠ΄Π΅ΡΠΆΠ°ΡΠΈΠ΅ ΠΊΡΠ΅ΠΌΠ½Π΅Π·Π΅ΠΌΠ½ΡΡ ΡΠΎΡΡΠ°Π²Π»ΡΡΡΡΡ (SiO2), ΠΎΠ±ΡΠ°Π·ΡΡΡΡΡΡΡ Π² ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠ΅ ΡΠ΅Π°ΠΊΡΠΈΠΉ Π³ΠΈΠ΄ΡΠΎΠ»ΠΈΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΏΠΎΠ»ΠΈΠΊΠΎΠ½Π΄Π΅Π½ΡΠ°ΡΠΈΠΈ (Π·ΠΎΠ»Ρ-Π³Π΅Π»Ρ-ΠΏΡΠΎΡΠ΅ΡΡΠ°) ΡΠ΅ΡΡΠ°ΠΌΠ΅ΡΠΎΠΊΡΠΈΡΠΈΠ»Π°Π½Π° (Π’ΠΠΠ‘), Π²Π²ΠΎΠ΄ΠΈΠΌΠΎΠ³ΠΎ Π² ΠΈΡΡ
ΠΎΠ΄Π½ΡΠΉ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠΈΡΠΎΠ²Π°Π½Π½ΡΠΉ ΡΠ°ΡΡΠ²ΠΎΡ ΠΏΠΎΠ»ΠΈΠΌΠ΅ΡΠ° Π² Π΄ΠΈΠΌΠ΅ΡΠΈΠ»ΡΡΠ»ΡΡΠΎΠΊΡΠΈΠ΄Π΅ (ΠΠΠ‘Π). Π‘ΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅ ΠΠΠ‘ Π² ΡΠ°ΠΊΠΈΡ
ΡΠ°ΡΡΠ²ΠΎΡΠ°Ρ
ΡΠΎΡΡΠ°Π²Π»ΡΠ»ΠΎ 60, 80 ΠΈΠ»ΠΈ 100 Π³/Π»; ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΡ Π’ΠΠΠ‘ Π²Π°ΡΡΠΈΡΠΎΠ²Π°Π»ΠΈ ΠΎΡ 0.15 Π΄ΠΎ 0.61 ΠΌΠΎΠ»Ρ/Π»; ΠΊΡΠΈΠΎΠ³Π΅Π½Π½ΡΡ ΠΎΠ±ΡΠ°Π±ΠΎΡΠΊΡ ΡΠ°ΡΡΠ²ΠΎΡΠΎΠ² ΠΏΠΎΠ»ΠΈΠΌΠ΅ΡΠ° ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ ΠΏΡΠΈ ΡΠ΅ΠΌΠΏΠ΅ΡΠ°ΡΡΡΠ΅, Π½Π° 40Β° Π½ΠΈΠΆΠ΅ ΡΠΎΡΠΊΠΈ ΠΊΡΠΈΡΡΠ°Π»Π»ΠΈΠ·Π°ΡΠΈΠΈ ΡΠΈΡΡΠΎΠ³ΠΎ ΠΠΠ‘Π (+18.4 Β°C); Π·Π°ΠΌΠΎΡΠΎΠΆΠ΅Π½Π½ΡΠ΅ ΠΎΠ±ΡΠ°Π·ΡΡ ΠΎΡΡΠ°ΠΈΠ²Π°Π»ΠΈ, Π½Π°Π³ΡΠ΅Π²Π°Ρ ΡΠΎ ΡΠΊΠΎΡΠΎΡΡΡΡ 0.03Β°/ΠΌΠΈΠ½. ΠΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ Π½Π΅Π³Π»ΡΠ±ΠΎΠΊΠΎΠ΅ Π·Π°ΠΌΠΎΡΠ°ΠΆΠΈΠ²Π°Π½ΠΈΠ΅ (β21.6 Β°C), Π²ΡΠ΄Π΅ΡΠΆΠΈΠ²Π°Π½ΠΈΠ΅ Π² Π·Π°ΠΌΠΎΡΠΎΠΆΠ΅Π½Π½ΠΎΠΌ ΡΠΎΡΡΠΎΡΠ½ΠΈΠΈ ΠΈ ΠΏΠΎΡΠ»Π΅Π΄ΡΡΡΠ΅Π΅ ΠΎΡΡΠ°ΠΈΠ²Π°Π½ΠΈΠ΅ ΠΈΡΡ
ΠΎΠ΄Π½ΠΎΠΉ ΡΠ΅Π°ΠΊΡΠΈΠΎΠ½Π½ΠΎΠΉ ΡΠΌΠ΅ΡΠΈ ΠΠΠ‘/ΠΠΠ‘Π/Π’ΠΠΠ‘/ΠΊΠΈΡΠ»ΠΎΡΠ½ΡΠΉ ΠΊΠ°ΡΠ°Π»ΠΈΠ·Π°ΡΠΎΡ ΠΏΡΠΈΠ²ΠΎΠ΄ΠΈΡ ΠΊ ΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΠΏΡΠΎΡΠ½ΡΡ
ΠΌΠ°ΠΊΡΠΎΠΏΠΎΡΠΈΡΡΡΡ
ΠΊΡΠΈΠΎΠ³Π΅Π»Π΅ΠΉ ΠΠΠ‘/SiO2. ΠΠΎΠ»ΡΡΠ΅Π½Π½ΡΠ΅ ΠΊΡΠΈΠΎΠ³Π΅Π»Π΅Π²ΡΠ΅ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ ΡΠ²Π»ΡΡΡΡΡ Π³ΠΈΠ±ΡΠΈΠ΄Π½ΡΠΌΠΈ Π² ΡΠ²ΡΠ·ΠΈ Ρ Π²ΠΎΠ·Π½ΠΈΠΊΠ½ΠΎΠ²Π΅Π½ΠΈΠ΅ΠΌ ΡΡΡΠΎΠΉΡΠΈΠ²ΡΡ
ΠΊΠΎΠ½ΡΠ°ΠΊΡΠΎΠ² ΠΌΠ°ΠΊΡΠΎΠΌΠΎΠ»Π΅ΠΊΡΠ»Ρ ΠΠΠ‘βΠΎΠ»ΠΈΠ³ΠΎΡΠΈΠ»ΠΎΠΊΡΠ°Π½Ρ, Π΄Π²ΠΈΠΆΡΡΠ΅ΠΉ ΡΠΈΠ»ΠΎΠΉ ΡΠ°ΠΊΠΎΠ³ΠΎ ΠΌΠ΅ΠΆΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΠΎΠ³ΠΎ Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΠΈΡ ΡΠ²Π»ΡΠ΅ΡΡΡ Π²ΠΎΠ΄ΠΎΡΠΎΠ΄Π½ΠΎΠ΅ ΡΠ²ΡΠ·ΡΠ²Π°Π½ΠΈΠ΅ ΠΌΠ΅ΠΆΠ΄Ρ ΠΠ-Π³ΡΡΠΏΠΏΠ°ΠΌΠΈ ΡΠΎΡΠ΅Π΄Π½ΠΈΡ
ΡΠ΅ΠΏΠ΅ΠΉ, ΠΏΡΠΈΠ²ΠΎΠ΄ΡΡΠ΅Π΅ ΠΊ ΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΡ ΠΌΠΎΠ΄ΠΈΡΠΈΡΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
Π·ΠΎΠ½ ΠΌΠΈΠΊΡΠΎΠΊΡΠΈΡΡΠ°Π»Π»ΠΈΡΠ½ΠΎΡΡΠΈ, Π²ΡΠΏΠΎΠ»Π½ΡΡΡΠΈΡ
ΡΡΠ½ΠΊΡΠΈΡ ΡΠ·Π»ΠΎΠ² ΠΏΡΠΎΡΡΡΠ°Π½ΡΡΠ²Π΅Π½Π½ΠΎΠΉ ΡΠ΅ΡΠΊΠΈ ΠΊΡΠΈΠΎΠ³Π΅Π»Π΅ΠΉ. Π£ΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½Ρ ΡΡΡΠ΅ΠΊΡΡ ΡΡΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎΠ³ΠΎ Π²ΠΎΠ·ΡΠ°ΡΡΠ°Π½ΠΈΡ ΠΆΠ΅ΡΡΠΊΠΎΡΡΠΈ ΠΈ ΡΠ΅ΠΏΠ»ΠΎΡΡΠΎΠΉΠΊΠΎΡΡΠΈ ΠΊΡΠΈΠΎΠ³Π΅Π»Π΅ΠΉ ΠΠΠ‘/SiO2 ΠΏΠΎ ΠΌΠ΅ΡΠ΅ ΠΏΠΎΠ²ΡΡΠ΅Π½ΠΈΡ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΠΈ ΠΠΠ‘ ΠΈ Π’ΠΠΠ‘ Π² ΠΈΡΡ
ΠΎΠ΄Π½ΠΎΠΉ ΡΠΈΡΡΠ΅ΠΌΠ΅. ΠΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ ΡΡΠΏΠ΅Ρ
ΡΠΈΠ½ΡΠ΅Π·Π° ΠΏΡΠΎΠ·ΡΠ°ΡΠ½ΡΡ
ΡΠΏΡΡΠ³ΠΈΡ
ΠΈ ΡΠ΅ΡΠΌΠΎΡΡΠΎΠΉΠΊΠΈΡ
ΠΊΡΠΈΠΎΠ³Π΅Π»Π΅ΠΉ ΠΠΠ‘/SiO2 Π·Π°Π²ΠΈΡΠΈΡ ΠΊΠ°ΠΊ ΠΎΡ Π½Π°Ρ
ΠΎΠΆΠ΄Π΅Π½ΠΈΡ ΠΎΠΏΡΠΈΠΌΠ°Π»ΡΠ½ΠΎΠ³ΠΎ ΡΠΎΠΎΡΠ½ΠΎΡΠ΅Π½ΠΈΡ ΠΌΠ΅ΠΆΠ΄Ρ ΠΈΡΡ
ΠΎΠ΄Π½ΡΠΌΠΈ Π²Π΅ΡΠ΅ΡΡΠ²Π°ΠΌΠΈ, ΡΠ°ΠΊ ΠΈ ΠΎΡ ΡΠΎΠ²ΠΌΠ΅ΡΡΠ½ΠΎΠ³ΠΎ Π²ΠΎΠ·Π΄Π΅ΠΉΡΡΠ²ΠΈΡ ΠΆΠΈΠ΄ΠΊΠΈΡ
(ΠΌΠ΅ΡΠ°Π½ΠΎΠ» ΠΈ Π²ΠΎΠ΄Π°) ΠΈ ΠΊΡΠ΅ΠΌΠ½ΠΈΠΉΡΠΎΠ΄Π΅ΡΠΆΠ°ΡΠΈΡ
ΠΊΠΎΠΌΠΏΠΎΠ½Π΅Π½ΡΠΎΠ² Π½Π° ΠΏΡΠΎΡΠ΅ΡΡΡ ΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΡ ΠΌΠ½ΠΎΠΆΠ΅ΡΡΠ²Π΅Π½Π½ΡΡ
Π²ΠΎΠ΄ΠΎΡΠΎΠ΄Π½ΡΡ
ΡΠ²ΡΠ·Π΅ΠΉ ΠΈ ΠΌΠΈΠΊΡΠΎΠΊΡΠΈΡΡΠ°Π»Π»ΠΈΡΠΎΠ².
Two-Photon Fluorescence Microscopy Imaging of Cellular Oxidative Stress Using Profluorescent Nitroxides
A range of varying chromophore nitroxide free radicals and their nonradical methoxyamine analogues were synthesized and their linear photophysical properties examined. The presence of the proximate free radical masks the chromophoreβs usual fluorescence emission, and these species are described as profluorescent. Two nitroxides incorporating anthracene and fluorescein chromophores (compounds 7 and 19, respectively) exhibited two-photon absorption (2PA) cross sections of approximately 400 G.M. when excited at wavelengths greater than 800 nm. Both of these profluorescent nitroxides demonstrated low cytotoxicity toward Chinese hamster ovary (CHO) cells. Imaging colocalization experiments with the commercially available CellROX Deep Red oxidative stress monitor demonstrated good cellular uptake of the nitroxide probes. Sensitivity of the nitroxide probes to H2O2-induced damage was also demonstrated by both one- and two-photon fluorescence microscopy. These profluorescent nitroxide probes are potentially powerful tools for imaging oxidative stress in biological systems, and they essentially βlight upβ in the presence of certain species generated from oxidative stress. The high ratio of the fluorescence quantum yield between the profluorescent nitroxide species and their nonradical adducts provides the sensitivity required for measuring a range of cellular redox environments. Furthermore, their reasonable 2PA cross sections provide for the option of using two-photon fluorescence microscopy, which circumvents commonly encountered disadvantages associated with one-photon imaging such as photobleaching and poor tissue penetration
ΠΠ½Π΄ΡΠΊΡΠΈΡ ΠΎΡΡΠ΅ΠΎΠ³Π΅Π½Π΅Π·Π° ΠΊΠΎΡΡΠ½ΠΎΠΉ ΡΠΊΠ°Π½ΠΈ Π½ΠΈΠΆΠ½Π΅ΠΉ ΡΠ΅Π»ΡΡΡΠΈ ΠΊΡΠΎΠ»ΠΈΠΊΠ° Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ ΠΊΡΠΈΠΎΠ³Π΅Π½Π½ΠΎβΡΡΡΡΠΊΡΡΡΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠ³ΠΎ Π³ΡΠ±ΡΠ°ΡΠΎΠ³ΠΎ Π°Π»ΡΠ±ΡΠΌΠΈΠ½ΠΎΠ²ΠΎΠ³ΠΎ 3D-Π½ΠΎΡΠΈΡΠ΅Π»Ρ, Π½Π°Π³ΡΡΠΆΠ΅Π½Π½ΠΎΠ³ΠΎ Π±ΠΈΠΎΡΠ΅Π³ΡΠ»ΡΡΠΎΡΠΎΠΌ
Objective: to study the induction of osteogenesis caused by introducing into the defect area broadly porous cryogenically structured 3D carriers, based on serum albumin and loaded with a bioregulator isolated from bovine serum on an experimental model of mandible defect in rabbits in vivo.Materials and methods. Cryogenically structured sponges in the form of cylindrical specimens, 5 mm in diameter and 5 mm in height, prepared from bovine serum albumin, were used as the bioregulator carrier. The experimental laboratory animals were male Chinchilla rabbits, weighing 2β2.5 kg. Bone tissue was skeletonized under anesthesia (intramuscular anesthetic Zoletil 100) with a 3-cm incision in the angle of the mandible and a 5-mm-diameter cutter was used to create a 2β3-mm deep defect to install an appropriate-size albumin sponge. A total of 24 animals participated in the experiment. X-ray control of the defect area was performed in vivo on day 14 using PanExam+ (Kavo) device (20 m X-ray). Histological examination of tissues was carried out at day 30 after the defect using a light microscope.Results. Experiments performed indicate an active restoration of bone tissue in the extensive defect area when using an albumin-based 3D carrier with the inclusion of a bioregulator as compared to the control experiments. There were osteointegrative and osteoinductive processes, almost complete decomposition (biodegradation) of albumin sponge with formation of islands of dense bone tissue with small foci of coarse fibrous tissue in the defect. This demonstrated good dynamics of recovery processes at this stage of healing.Conclusion. Under the action of a serum bioregulator contained in an albumin-based sponge, the repair process leads to restoration of normal bone tissue without formation of bone callus and altered bone tissue different from the native one.Π¦Π΅Π»Ρ ΡΠ°Π±ΠΎΡΡ: ΠΈΠ·ΡΡΠΈΡΡ in vivo Π½Π° ΠΌΠΎΠ΄Π΅Π»ΠΈ ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ°Π»ΡΠ½ΠΎΠ³ΠΎ ΠΊΠΎΡΡΠ½ΠΎΠ³ΠΎ Π΄Π΅ΡΠ΅ΠΊΡΠ° Π½ΠΈΠΆΠ½Π΅ΠΉ ΡΠ΅Π»ΡΡΡΠΈ ΠΊΡΠΎΠ»ΠΈΠΊΠ° ΠΈΠ½Π΄ΡΠΊΡΠΈΡ ΠΎΡΡΠ΅ΠΎΠ³Π΅Π½Π΅Π·Π°, Π²ΡΠ·ΡΠ²Π°Π΅ΠΌΡΡ Π²Π½Π΅ΡΠ΅Π½ΠΈΠ΅ΠΌ Π² ΠΎΠ±Π»Π°ΡΡΡ Π΄Π΅ΡΠ΅ΠΊΡΠ° ΡΠΈΡΠΎΠΊΠΎΠΏΠΎΡΠΈΡΡΡΡ
ΠΊΡΠΈΠΎΠ³Π΅Π½Π½ΠΎ-ΡΡΡΡΠΊΡΡΡΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
3D-Π½ΠΎΡΠΈΡΠ΅Π»Π΅ΠΉ Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ ΡΡΠ²ΠΎΡΠΎΡΠΎΡΠ½ΠΎΠ³ΠΎ Π°Π»ΡΠ±ΡΠΌΠΈΠ½Π°, Π½Π°Π³ΡΡΠΆΠ΅Π½Π½ΡΡ
Π±ΠΈΠΎΡΠ΅Π³ΡΠ»ΡΡΠΎΡΠΎΠΌ, Π²ΡΠ΄Π΅Π»Π΅Π½Π½ΡΠΌ ΠΈΠ· ΡΡΠ²ΠΎΡΠΎΡΠΊΠΈ ΠΊΡΠΎΠ²ΠΈ ΠΊΡΡΠΏΠ½ΠΎΠ³ΠΎ ΡΠΎΠ³Π°ΡΠΎΠ³ΠΎ ΡΠΊΠΎΡΠ°.ΠΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ. Π ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅ Π½ΠΎΡΠΈΡΠ΅Π»Ρ Π±ΠΈΠΎΡΠ΅Π³ΡΠ»ΡΡΠΎΡΠ° ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π»ΠΈ ΠΊΡΠΈΠΎΠ³Π΅Π½Π½ΠΎ-ΡΡΡΡΠΊΡΡΡΠΈΡΠΎΠ²Π°Π½Π½ΡΠ΅ Π³ΡΠ±ΠΊΠΈ Π² Π²ΠΈΠ΄Π΅ ΡΠΈΠ»ΠΈΠ½Π΄ΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΎΠ±ΡΠ°Π·ΡΠΎΠ² Π΄ΠΈΠ°ΠΌΠ΅ΡΡΠΎΠΌ 5 ΠΌΠΌ ΠΈ Π²ΡΡΠΎΡΠΎΠΉ ΡΠ°ΠΊΠΆΠ΅ 5 ΠΌΠΌ, ΠΏΡΠΈΠ³ΠΎΡΠΎΠ²Π»Π΅Π½Π½ΡΠ΅ ΠΈΠ· Π±ΡΡΡΠ΅Π³ΠΎ ΡΡΠ²ΠΎΡΠΎΡΠΎΡΠ½ΠΎΠ³ΠΎ Π°Π»ΡΠ±ΡΠΌΠΈΠ½Π°. ΠΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΡ Ρ Π»Π°Π±ΠΎΡΠ°ΡΠΎΡΠ½ΡΠΌΠΈ ΠΆΠΈΠ²ΠΎΡΠ½ΡΠΌΠΈ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ Π½Π° ΠΊΡΠΎΠ»ΠΈΠΊΠ°Ρ
ΠΏΠΎΡΠΎΠ΄Ρ Π¨ΠΈΠ½ΡΠΈΠ»Π»Π° Π²Π΅ΡΠΎΠΌ 2β2,5 ΠΊΠ³, ΡΠ°ΠΌΡΠ°Ρ
. ΠΠΎΠ΄ Π½Π°ΡΠΊΠΎΠ·ΠΎΠΌ (Π²Π½ΡΡΡΠΈΠΌΡΡΠ΅ΡΠ½ΡΠΉ Π½Π°ΡΠΊΠΎΠ· Zoletil 100) ΡΠ°Π·ΡΠ΅Π·ΠΎΠΌ Π΄ΠΎ 3 ΡΠΌ Π² ΠΎΠ±Π»Π°ΡΡΠΈ ΡΠ³Π»Π° Π½ΠΈΠΆΠ½Π΅ΠΉ ΡΠ΅Π»ΡΡΡΠΈ ΡΠΊΠ΅Π»Π΅ΡΠΈΡΠΎΠ²Π°Π»ΠΈ ΠΊΠΎΡΡΠ½ΡΡ ΡΠΊΠ°Π½Ρ ΠΈ ΡΡΠ΅Π·ΠΎΠΉ Π΄ΠΈΠ°ΠΌΠ΅ΡΡΠΎΠΌ 5 ΠΌΠΌ ΡΠΎΠ·Π΄Π°Π²Π°Π»ΠΈ Π΄Π΅ΡΠ΅ΠΊΡ Π³Π»ΡΠ±ΠΈΠ½ΠΎΠΉ 2β3 ΠΌΠΌ Π΄Π»Ρ ΡΡΡΠ°Π½ΠΎΠ²ΠΊΠΈ Π°Π»ΡΠ±ΡΠΌΠΈΠ½ΠΎΠ²ΠΎΠΉ Π³ΡΠ±ΠΊΠΈ ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²ΡΡΡΠ΅Π³ΠΎ ΡΠ°Π·ΠΌΠ΅ΡΠ°. ΠΡΠ΅Π³ΠΎ Π² ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ΅ ΠΏΡΠΈΡΡΡΡΡΠ²ΠΎΠ²Π°Π»ΠΎ 24 ΠΆΠΈΠ²ΠΎΡΠ½ΡΡ
. ΠΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ ΡΠ΅Π½ΡΠ³Π΅Π½ΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠΉ ΠΊΠΎΠ½ΡΡΠΎΠ»Ρ ΠΎΠ±Π»Π°ΡΡΠΈ Π΄Π΅ΡΠ΅ΠΊΡΠ° Π½Π° 14-Π΅ ΡΡΡΠΊΠΈ ΠΏΡΠΈΠΆΠΈΠ·Π½Π΅Π½Π½ΠΎ Π½Π° Π°ΠΏΠΏΠ°ΡΠ°ΡΠ΅ PanExam+ (Kavo), 20 ΠΌΠ Π΅Π½ΡΠ³Π΅Π½. ΠΠΈΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠ΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ ΡΠΊΠ°Π½Π΅ΠΉ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ Π½Π° 30-Π΅ ΡΡΡΠΊΠΈ ΠΏΠΎΡΠ»Π΅ Π½Π°Π½Π΅ΡΠ΅Π½ΠΈΡ Π΄Π΅ΡΠ΅ΠΊΡΠ° Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ ΡΠ²Π΅ΡΠΎΠ²ΠΎΠ³ΠΎ ΠΌΠΈΠΊΡΠΎΡΠΊΠΎΠΏΠ°.Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½Π½ΡΡ
ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠΎΠ² ΡΠ²ΠΈΠ΄Π΅ΡΠ΅Π»ΡΡΡΠ²ΡΡΡ ΠΎΠ± Π°ΠΊΡΠΈΠ²Π½ΠΎΠΌ Π²ΠΎΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΈΠΈ ΠΊΠΎΡΡΠ½ΠΎΠΉ ΡΠΊΠ°Π½ΠΈ Π² ΠΎΠ±Π»Π°ΡΡΠΈ ΠΎΠ±ΡΠΈΡΠ½ΠΎΠ³ΠΎ Π΄Π΅ΡΠ΅ΠΊΡΠ° ΠΏΡΠΈ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠΈ Π°Π»ΡΠ±ΡΠΌΠΈΠ½ΠΎΠ²ΠΎΠ³ΠΎ 3D-Π½ΠΎΡΠΈΡΠ΅Π»Ρ ΠΈΠΌΠ΅Π½Π½ΠΎ Ρ Π²ΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅ΠΌ Π±ΠΈΠΎΡΠ΅Π³ΡΠ»ΡΡΠΎΡΠ° ΠΏΠΎ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ Ρ ΠΊΠΎΠ½ΡΡΠΎΠ»ΡΠ½ΡΠΌΠΈ ΠΎΠΏΡΡΠ°ΠΌΠΈ. ΠΠ°ΡΠ΅Π³ΠΈΡΡΡΠΈΡΠΎΠ²Π°Π½Ρ ΠΏΡΠΎΡΠ΅ΡΡΡ ΠΎΡΡΠ΅ΠΎΠΈΠ½ΡΠ΅Π³ΡΠ°ΡΠΈΠ²Π½ΠΎΠΉ ΠΈ ΠΎΡΡΠ΅ΠΎΠΈΠ½Π΄ΡΠΊΡΠΈΠ²Π½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ, ΠΏΡΠ°ΠΊΡΠΈΡΠ΅ΡΠΊΠΈ ΠΏΠΎΠ»Π½ΠΎΠ΅ ΡΠ°Π·Π»ΠΎΠΆΠ΅Π½ΠΈΠ΅ (Π±ΠΈΠΎΠ΄Π΅Π³ΡΠ°Π΄Π°ΡΠΈΡ) Π°Π»ΡΠ±ΡΠΌΠΈΠ½ΠΎΠ²ΠΎΠΉ Π³ΡΠ±ΠΊΠΈ Ρ ΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ Π½Π° ΠΌΠ΅ΡΡΠ΅ Π΄Π΅ΡΠ΅ΠΊΡΠ° ΠΎΡΡΡΠΎΠ²ΠΊΠΎΠ² ΠΏΠ»ΠΎΡΠ½ΠΎΠΉ ΠΊΠΎΡΡΠ½ΠΎΠΉ ΡΠΊΠ°Π½ΠΈ Ρ Π½Π΅Π±ΠΎΠ»ΡΡΠΈΠΌΠΈ ΠΎΡΠ°Π³Π°ΠΌΠΈ Π³ΡΡΠ±ΠΎΠ²ΠΎΠ»ΠΎΠΊΠ½ΠΈΡΡΠΎΠΉ ΡΠΊΠ°Π½ΠΈ, ΡΡΠΎ Π³ΠΎΠ²ΠΎΡΠΈΡ ΠΎ Ρ
ΠΎΡΠΎΡΠ΅ΠΉ Π΄ΠΈΠ½Π°ΠΌΠΈΠΊΠ΅ Π²ΠΎΡΡΡΠ°Π½ΠΎΠ²ΠΈΡΠ΅Π»ΡΠ½ΡΡ
ΠΏΡΠΎΡΠ΅ΡΡΠΎΠ² Π½Π° Π΄Π°Π½Π½ΠΎΠΌ ΡΡΠΎΠΊΠ΅ Π·Π°ΠΆΠΈΠ²Π»Π΅Π½ΠΈΡ.ΠΠ°ΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅. ΠΠΎΠ»ΡΡΠ΅Π½Π½ΡΠ΅ Π΄Π°Π½Π½ΡΠ΅ ΡΠΊΠ°Π·ΡΠ²Π°ΡΡ Π½Π° ΡΠΎ, ΡΡΠΎ ΠΏΠΎΠ΄ Π΄Π΅ΠΉΡΡΠ²ΠΈΠ΅ΠΌ ΡΡΠ²ΠΎΡΠΎΡΠΎΡΠ½ΠΎΠ³ΠΎ Π±ΠΈΠΎΡΠ΅Π³ΡΠ»ΡΡΠΎΡΠ° Π² ΡΠΎΡΡΠ°Π²Π΅ Π°Π»ΡΠ±ΡΠΌΠΈΠ½ΠΎΠ²ΠΎΠΉ Π³ΡΠ±ΠΊΠΈ ΠΏΡΠΎΡΠ΅ΡΡ ΡΠ΅ΠΏΠ°ΡΠ°ΡΠΈΠΈ ΠΏΡΠΈΠ²ΠΎΠ΄ΠΈΡ ΠΊ Π²ΠΎΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΈΡ Π½ΠΎΡΠΌΠ°Π»ΡΠ½ΠΎΠΉ ΠΊΠΎΡΡΠ½ΠΎΠΉ ΡΠΊΠ°Π½ΠΈ Π±Π΅Π· ΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΠΊΠΎΡΡΠ½ΠΎΠΉ ΠΌΠΎΠ·ΠΎΠ»ΠΈ ΠΈ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½Π½ΠΎΠΉ ΠΊΠΎΡΡΠ½ΠΎΠΉ ΡΠΊΠ°Π½ΠΈ, ΠΎΡΠ»ΠΈΡΠ½ΠΎΠΉ ΠΎΡ Π½Π°ΡΠΈΠ²Π½ΠΎΠΉ ΡΠΊΠ°Π½ΠΈ
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