Light-sheet microscopy: a tutorial

This paper is intended to give a comprehensive review of light-sheet (LS) microscopy from an optics perspective. As such, emphasis is placed on the advantages that LS microscope configurations present, given the degree of freedom gained by uncoupling the excitation and detection arms. The new imaging properties are first highlighted in terms of optical parameters and how these have enabled several biomedical applications. Then, the basics are presented for understanding how a LS microscope works. This is followed by a presentation of a tutorial for LS microscope designs, each working at different resolutions and for different applications. Then, based on a numerical Fourier analysis and given the multiple possibilities for generating the LS in the microscope (using Gaussian, Bessel, and Airy beams in the linear and nonlinear regimes), a systematic comparison of their optical performance is presented. Finally, based on advances in optics and photonics, the novel optical implementations possible in a LS microscope are highlighted.Peer ReviewedPostprint (published version

DIONISIO 3.0: Comprehensive 3D nuclear fuel simulation through PCMI cohesive and PLENUM models

We present a new version of the DIONISIO code, extending its capabilities to three-dimensional domains. Adding to the functionality that divides the rod in a user-defined number of segments, the user can now choose the dimensionality of the domain in which a representative pellet-gap-cladding system is solved. To achieve this, we have developed a new algorithm to simulate the contact between pellet and cladding based in Cohesive Finite Elements, a natural mode to approach this issue. We present our results testing this kind of contact element in order to validate the concept. Alongside the contact algorithm, we have included a model of the plenum domain in three dimensions, treated using an external FEM mesh created ad hoc. DIONISIO reads this mesh adapting it to the specific case to be simulated and solves the energy equation inside the plenum following specific boundary conditions. We compare the new models to selected experiments under irradiation, in normal or accident conditions, for validation, with results showing a high correlation with said experiments.Fil: Goldberg, Ezequiel. Comision Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. Gerencia Ciclo del Combustible Nuclear; Argentina. Universidad Nacional de San Martín. Instituto Sabato; ArgentinaFil: Loza Peralta, Matías E.. Comision Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. Gerencia Ciclo del Combustible Nuclear; Argentina. Universidad Nacional de San Martín. Instituto Sabato; ArgentinaFil: Soba, Alejandro. Comision Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. Gerencia Ciclo del Combustible Nuclear; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

Effect of Enogen Feed Corn Inclusion in Conventional and Natural Finishing Cattle Diets

Increasing the extent of starch digestibility during finishing could allow producers to improve cattle efficiency. A finishing performance study was conducted to determine the effect of Enogen Feed Corn inclusion as dry-rolled corn and corn silage in comparison to a control corn hybrid within natural and conventional feeding programs for heifers and steers. Cattle in the conventional feeding program received implants and the ration included feed additives, while cattle on the natural program were not implanted and the ration did not contain feed additives. The inclusion of Enogen Feed Corn had no impact on steer or heifer finishing performance. The use of implants and feed additives in the conventional feeding program increased hot carcass weight 12.2% in steers and 7.0% in heifers. When compared to cattle in the natural program, feeding cattle in a conventional program improved feed conversion by 19.4% in steers and 13.0% in heifers

Latinas in the Legal Academy: Progress and Promise

The 2022 Inaugural Graciela Oliva ́rez Latinas in the Legal Academy (“GO LILA”) Workshop convened seventy-four outstanding and powerful Latina law professors and professional legal educators (collectively, “Latinas in the legal academy,” or “LILAs”) to document and celebrate our individual and collective journeys and to grow stronger together. In this essay, we, four of the Latina law professors who helped to co-found the GO LILA Workshop, share what we learned about and from each other. We invite other LILAs to join our community and share their stories and journeys. We hope that the data and lessons that we share can inspire other Latinas to join the legal academy. We encourage law schools to honor the transformation that our presence and contributions have brought to legal education and scholarship and to join us in considering how our path forward can be even more impactful and sustaining

Variabilité spatiale de la teneur en eau de surface des sols nus par mesures in situ et imagerie radar

National audienceOn présente l'analyse géostatistique de la teneur en eau de surface (0-6 cm de profondeur) collectée les 12 et 13 Mars 2009, sur une quinzaine de parcelles de sol nu d'un petit bassin péri-urbain proche de Lyon. Les mesures in situ, ont été collectées à deux échelles : une échelle locale sur des croix de longueur 20m et un pas d'espace de 1m et une échelle parcellaire sur 3 transects avec un pas de 20m environ. Les résultats montrent une corrélation de quelques m à échelle fine et de 20 à 50m à l'échelle de la parcelle. Après correction du bruit, calibration radiométrique et correction des effets géométriques et de pente, la comparaison des moyennes par parcelles issues de l'image radar TerraSAR-X et des mesures in situ est satisfaisante (R2=0.43) mais l'analyse intra-parcellaire reste à affiner. / This paper presents the geostatistical analysis of surface soil water content (0-6 cm depth), collected on March 12-13 2009, in about 15 bare soil fields located in a small suburban catchment close to Lyon. In situ data were sampled at two scales : a local scale on 20m-long crosses with a space step of about 1m; a field scale, with 3 transects and a space scale of about 20m. The results show a correlation of a few meters at the local scale and of about 20-50m at the field scale. After correction of the noise, radiometric calibration, geometric and slope effect correction, the comparison of the field averages derived from the TerraSAR-X image and of in situ data is satisfactory (R2=0.43), but the intra-field variability should be studied in more details

Evaluation of Vivalto\u3csup\u3e®\u3c/sup\u3e on Growth Performance and Carcass Characteristics in Growing and Finishing Beef Steers

A finishing trial conducted at the Pan-handle Research, Extension, and Education Center (PREEC) near Scottsbluff, NE evaluated the effect of Vivalto®, a B-vitamin complex, on the feeding performance and carcass characteristics of feedlot steers. The design of the study was completely randomized block design with three treatments, control without Vivalto®, 1g Vivalto®/steer/day, and 2g Vivalto®/steer/day. Cattle fed the diets that included Vivalto® feed had lower intakes during the first 56 days than cattle that received no Vivalto® in the diet. No significant differences were observed for ADG, intake, feed efficiency and carcass characteristics of the entire feeding period. No significant differences in prevalence of liver abscesses were found between treatments and prevalance of A+ abscesses was also insignificant

Comparison of Corn Silage and Earlage in Finishing Diets when fed as a Roughage on a Neutral Detergent Fiber Basis

The finishing trial was conducted to evaluate the ability of earlage to serve as a roughage source compared to corn silage when both sources were balanced on an NDF basis. Steers were fed in a randomized block design with a 2x2 treatment arrangement, with one factor being NDF source (corn silage or earlage) and the other factor being inclusion ( normal amount of roughage provided by corn silage or earlage as the only grain source and roughage source). No significant interactions were observed between roughage source and inclusion level on steer performance. Steers fed corn silage and grain had greater intakes and gains than steers fed earlage. Steers fed less NDF had more efficient feed conversion, and greater gain with heavier hot carcass weights