Variations of the Milankovitch frequencies in time

The sensitivity of the amplitudes and frequencies in the development of the Earth's orbital and rotational elements involved in the astronomical theory of paleoclimates (eccentricity, obliquity, and climate precession), to the Earth-Moon distance and consequently to the length of the day and to the dynamical ellipticity of the Earth has been discussed for the last billions of years. The shortening of the Earth-Moon distance and of the length of the day, as well as the lengthening of the dynamical ellipticity of the Earth back in time induce a shortening of the fundamental astronomical periods for precession and obliquity. At the same time, the amplitudes of the different terms in the development of the obliquity are undergoing a relative enlargement of about 50 percent at 2 x 10(exp 9) yr BP but the independent term is increasing very weakly (less than 0.1 percent). In other words, the value of the obliquity, which lies within a range of 21.7 to 24.9 deg over the Quarternary was restricted to a range of 22.5 to 24.1 deg at 2 x 10(exp 9) yr BP. On the other hand, the amplitudes in the development of the climatic precession do not change. Moreover, these changes in the frequencies and amplitudes for both obliquity and climatic precession are larger for longer period terms. Finally, the periods in the eccentricity development are not influenced by the variation of the lunar distance. But the motion of the solar system, especially of the inner planets, was shown to be chaotic. It means that it is impossible to compute the exact motion of the planets over more than about 100 Myr, and the fundamental frequencies of the systems are not fixed quantities, but are slowly varying with time. As long as we consider the most important terms, the maximum deviation from the present-day value of the 19-kyr precessional period due to the chaotic motion of the solar system only does not reach more than a few tens of years around 80 Myr BP. Therefore the shortening of the obliquity and climatic precession periods is mostly driven by the change in the lunar distance and the consequent variations in the dynamical ellipticity of the Earth's angular speed. At first sight, the deviation in the period for the eccentricity can be neglected, as the chaotic behavior of the solar system implies a relative change of the main periods by less than 0.2 percent, 1.4 percent, and 1.9 percent respectively, this maximum change being achieved around 80 Myr BP. This implies, in particular, that the eccentricity periods for Quarternary climate studies may be considered more or less constant for pre-Quaternay times and equal to their Quaternary values

Glucosylation of xenobiotics in maize, soybean and arabidopsis thaliana

Many natural products and xenobiotics become glucosylated in the course of their metabolism in plants. This reaction is catalysed by type 1 UDP-glucose dependent glucosyltransferases (GTs), a super-family of enzymes which differ in their substrate specificity and which are able to glucosylate hydroxy, amino and carboxylic acid groups to form conjugates with altered bioactivities as compared with the parent aglycones. This study has focused both on NGT and OGT enzymes active towards amino and hydroxy groups, respectively, present in natural products and pesticide metabolites in two major crops (Zea mays and Glycine max) and the model plant Arabidopsis thaliana. A sensitive radioactive enzyme assay was developed to monitor conjugating activity in vitro and the substrate specificity of N-GTs and O-GTs determined in the three plant species with respect to xenobiotic detoxification. 3,4-Dichloroaniline was found to be the optimal N-GT substrate and 2,4,5-trichlorophenol the preferred 0-GT substrate in all the species tested. In addition, O-GT activities were also determined with other phenols of both natural and synthetic origin. To confirm the importance of N-GTs and O-GTs in xenobiotic detoxification, plant metabolism studies were carried out with [(^14)C]-p-nitrophenol and [(^14)C]-3,4-dichloroaniline. In each case, O- I N-glucosylation was found to be a major route of detoxification respectively. To determine whether or not herbicide-safeners could enhance the glucosylation of xenobiotics as had been demonstrated for the S-glutathionylation of herbicides in cereals; soybean, maize and Arabidopsis were treated with a range of safeners. The plants were then either fed with radiolabelled [(^14)C]-3,4-dichloroaniline, or extracted and assayed for O-GT and N-GT activities. In all species, safener treatment had no significant effect on the rate of uptake of radioactivity following feeding with [(^14)C]-3,4-dichloroaniline. However, specific safeners were found to enliance N-GT and O-GT activities in etiolated shoots, roots or suspension cultures in all species where tested. Several attempts were made to clone GT enzymes from soybean and maize based on a combination of bioinformatic and PCR approaches, the latter using conserved blocks of sequence in type 1 plant GTs to amplify up partial cDNAs. Using PCR and analysis of soybean expressed tagged accessions, it was possible to assemble a full-length cDNA from soybean which encoded a GT resembling an arbutin synthase GT from Rauvolfia serpentina. Although the resulting GT (GmGT32_l) could be expressed as a recombinant polypeptide in E.coli, the resulting protein was inactive and accumulated in the insoluble inclusion bodies. In the case of maize, a GT termed Z/wRP was identified as a random sequenced clone from a proprietary maize cDNA library. However, ZmRP could not be translated into protein using bacterial expression systems. Instead, an alternative proteomics approach at isolating plant GTs involved in xenobiotic detoxification was undertaken in Arabidopsis, using suspension cultured cells as the starting material. The major N-GT conjugating activity towards 3,4-dichloroaniline was purified 9552-fold using a combination of hydrophobic interaction, ion exchange, and affinity chromatographies. The resulting 50 kDa polypeptide was digested with trypsin and the peptide fragments analysed by MALDI TOF MS. Database analysis unambiguously identified the Arabidopsis protein as UGT72B1 (NM 116337). Following the completion of the Ph.D. programme the activity of GT72B1 towards 3,4-dichloroaniline, 2,4,5-trichlorophenol and other xenobiotics was confirmed and an account of the studies earned out on Arabidopsis GTs published (Lao, et al., 2003), (Loutre, et al., 2003)

On the importance of initial conditions for simulations of the Mid-Holocene climate.

Three simulations of the Mid-Holocene (6 ka) climate were performed with the ECBilt-CLIO-VECODE coupled atmosphere-ocean-vegetation model to study the impact of initial conditions. These experiments were forced with identical 6 ka forcings (orbital parameters and atmospheric greenhouse gas concentrations) and differed only in initial conditions. Two simulations were designed as equilibrium experiments, with one being initialized with preindustrial conditions as required by the protocol of the Paleoclimate Modelling Intercomparison Project (PMIP), while in a second experiment early Holocene (9 ka) initial conditions were used. These equilibrium simulations were run for 2100 years with 6 ka forcings. The third experiment was set up as a transient simulation, also starting from early Holocene conditions, but forced with annually changing orbital parameters and greenhouse gas levels. The results of the last 100 years are compared and reveal no statistically significant differences, showing that in this model the initial conditions have no discernible impact on the 6 ka climate. This suggests that the PMIP set-up for 6 ka simulations is valid, with the condition that spin-up phase should be long enough (at least 550 years) to allow the deep ocean to adjust to the change in forcings

Climate of the last millennium: a sensitivity study

Seventy-one sensitivity experiments have been performed using a two-dimensional sector-averaged global climate model to assess the potential impact of six different factors on the last millennium climate and in particular on the surface air temperature evolution. Both natural (i.e, solar and volcanism) and anthropogenically-induced (i.e. deforestation, additional greenhouse gases, and tropospheric aerosol burden) climate forcings have been considered.Comparisons of climate reconstructions with model results indicate that all the investigated forcings are needed to simulate the surface air temperature evolution. Due to uncertainties in historical climate forcings and temperature reconstructions, the relative importance of a particular forcing in the explanation of the recorded temperature variance is largely function of the forcing time series used. Nevertheless, our results indicate that whatever the historical solar and volcanic reconstructions may be, these externally driven natural climate forcings are unable to dive climate responses comparable in magnitude and time to the late-20th-century temperature warming while for earlier periods combination of solar and volcanic forcings can explain the Little Ice Age and the Medieval Warm Period. Only the greenhouse gas forcing allows the model to simulate an accelerated warming rate during the last three decades. The best guess simulation (largest similarity with the reconstruction) for the period starting 1850 AD requires however to include anthropogenic sulphate forcing as well as the impact of deforestation to constrain the magnitude of the greenhouse gas twentieth century warming to better fit the observation. On the contrary, prior to 1850 AD mid-latitude land clearance tends to reinforce the Little Ice age in our simulations

Equatorial insolation: from precession harmonics to eccentricity frequencies

Since the paper by Hays et al. (1976), spectral analyses of climate proxy records provide substantial evidence that a fraction of the climatic variance is driven by insolation changes in the frequency ranges of obliquity and precession variations. However, it is the variance components centered near 100 kyr which dominate most Upper Pleistocene climatic records, although the amount of insolation perturbation at the eccentricity driven periods close to 100-kyr (mainly the 95 kyr- and 123 kyr-periods) is much too small to cause directly a climate change of ice-age amplitude. Many attempts to find an explanation to this 100-kyr cycle in climatic records have been made over the last decades. Here we show that the double maximum which characterizes the daily irradiation received in tropical latitudes over the course of the year is at the origin in equatorial insolation of not only strong 95 kyr and 123 kyr periods related to eccentricity, but also of a 11-kyr and a 5.5-kyr periods related to precession

Factors controlling the last interglacial climate as simulated by LOVECLIM1.3

The last interglacial (LIG), also identified to the Eemian in Europe, began at approximately 130 kyr BP and ended at about 115 kyr BP (before present). More and more proxy-based reconstructions of the LIG climate are becoming more available even though they remain sparse. The major climate forcings during the LIG are rather well known and therefore models can be tested against paleoclimatic data sets and then used to better understand the climate of the LIG. However, models are displaying a large range of responses, being sometimes contradictory between them or with the reconstructed data. Here we would like to investigate causes of these differences. We focus on a single climate model, LOVECLIM, and we perform transient simulations over the LIG, starting at 135 kyr BP and run until 115 kyr BP. With these simulations, we test the role of the surface boundary conditions (the time-evolution of the Northern Hemisphere (NH) ice sheets) on the simulated LIG climate and the importance of the parameter sets (internal to the model, such as the albedos of the ocean and sea ice), which affect the sensitivity of the model. The magnitude of the simulated climate variations through the LIG remains too low compared to reconstructions for climate variables such as surface air temperature. Moreover, in the North Atlantic, the large increase in summer sea surface temperature towards the peak of the interglacial occurs too early (at ∼128 kyr BP) compared to the reconstructions. This feature as well as the climate simulated during the optimum of the LIG, between 131 and 121 kyr BP, does not depend on changes in surface boundary conditions and parameter sets. The additional freshwater flux (FWF) from the melting NH ice sheets is responsible for a temporary abrupt weakening of the North Atlantic meridional overturning circulation, which causes a strong global cooling in annual mean. However, the changes in the configuration (extent and albedo) of the NH ice sheets during the LIG only slightly impact the simulated climate. Together, configuration of and FWF from the NH ice sheets greatly increase the magnitude of the temperature variations over continents as well as over the ocean at the beginning of the simulation and reduce the difference between the simulated climate and the reconstructions. Lastly, we show that the contribution from the parameter sets to the climate response is actually very modest

The Eurasian ice sheet reinforces the East Asian summer monsoon during the interglacial 500 000 years ago

Deep-sea and ice-core records show that interglacial periods were overall less "warm" before about 420 000 years ago than after, with relatively higher ice volume and lower greenhouse gases concentration. This is particularly the case for the interglacial Marine Isotope Stage 13 which occurred about 500 000 years ago. However, by contrast, the loess and other proxy records from China suggest an exceptionally active East Asian summer monsoon during this interglacial. A three-dimension Earth system Model of Intermediate complexity was used to understand this seeming paradox. The astronomical forcing and the remnant ice sheets present in Eurasia and North America were taken into account in a series of sensitivity experiments. Expectedly, the seasonal contrast is larger and the East Asian summer monsoon is reinforced compared to Pre-Industrial time when Northern Hemisphere summer is at perihelion. Surprisingly, the presence of the Eurasian ice sheet was found to reinforce monsoon, too, through a south-eastwards perturbation planetary wave. The trajectory of this wave is influenced by the Tibetan plateau

How can a glacial inception be predicted?

The Early Anthropogenic Hypothesis considers that greenhouse gas concentrations should have declined during the Holocene in absence of humankind activity, leading to glacial inception around the present. It partly relies on the fact that present levels of northern summer incoming solar radiation are close to those that, in the past, preceded a glacial inception phenomenon, associated to declines in greenhouse gas concentrations. However, experiments with various numerical models of glacial cycles show that next glacial inception may still be delayed by several ten thousands of years, even with the assumption of greenhouse gas concentration declines during the Holocene. Furthermore, as we show here, conceptual models designed to capture the gross dynamics of the climate system as a whole suggest also that small disturbances may sometimes cause substantial delays in glacial events, causing a fair level of unpredictability on ice age dynamics. This suggests the need of a validated mathematical description of the climate system dynamics that allows us to quantify uncertainties on predictions. Here, it is proposed to organise our knowledge about the physics and dynamics of glacial cycles through a Bayesian inference network. Constraints on the physics and dynamics of climate can be encapsulated into a stochastic dynamical system. These constraints include, in particular, estimates of the sensitivity of the components of climate to external forcings, inferred from plans of experiments with large simulators of the atmosphere, oceans and ice sheets. On the other hand, palaeoclimate observations are accounted for through a process of parameter calibration. We discuss promises and challenges raised by this programme.Comment: Contribution to the special issue of 'The Holocene' on the Early Anthropogenic Hypotheses. W.R. Ruddiman, M. Crucifix, F. Oldfiel