Efeito das sobras de mandioca no ganho de peso de fêmeas bovinas e bubalinas.

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Carcass quality of buffalo (Bubalus bubalis) finished in silvopastoral system in the Eastern Amazon, Brazil.

Avaliou-se a influência da dieta sobre a qualidade de carcaça de búfalos mestiços das raças Murrah e Mediterrâneo, alimentados com concentrado tradicional milho/soja ou resíduos agroindustriais tortas de coco e dendê. Os animais foram sacrificados em abatedouro comercial, e realizadas análises físicas e físico-químicas na carcaça, após 24 horas, e de cor e textura instrumentais no músculo Longissimus dorsi, após 48 horas de resfriamento. Os animais suplementados com a torta de dendê adquiriram melhor conformação de carcaça e acabamento de gordura, e observaram-se diferenças (P<0,05) no percentual de gordura na carcaça e na espessura de gordura de cobertura

Limited risk of Zika virus transmission by five Aedes albopictus populations from Spain

Background: Aedes albopictus, the Asian tiger mosquito, is an exotic invasive species in Europe. It has substantial public health relevance due to its potential role in transmitting several human pathogens. Out of the European countries, Spain has one of the highest risk levels of autochthonous arbovirus transmission due to both the high density of Ae. albopictus and the extensive tourist influx from vector-endemic areas. This study aims to investigate the susceptibility of five Ae. albopictus populations from mainland Spain and the Balearic Islands to a Brazilian Zika virus (ZIKV) strain. Methods: The F1 generation of each Ae. albopictus population was orally challenged with a ZIKV-infected blood meal (1.8 × 10 PFU/ml). At 7 and 14 days post-infection (dpi), mosquito bodies (thorax and abdomen) and heads were individually analysed through RT-qPCR to determine the infection rate (IR) and dissemination rate (DR), respectively. The saliva of infected mosquitoes was inoculated in Vero cells and the transmission rate was assessed by plaque assay or RT-qPCR on ∼33 individuals per population. Results: The IR and DR ranged between 12-88%, and 0-60%, respectively, suggesting that ZIKV is capable of crossing the midgut barrier. Remarkably, no infectious viral particle was found in saliva samples, indicating a low ability of ZIKV to overcome the salivary gland barrier. A subsequent assay revealed that a second non-infective blood meal 48 h after ZIKV exposure did not influence Ae. albopictus vector competence. Conclusions: The oral experimental ZIKV infections performed here indicate that Ae. albopictus from Spain become infected and disseminate the virus through the body but has a limited ability to transmit the Brazilian ZIKV strain through biting. Therefore, the results suggest a limited risk of autochthonous ZIKV transmission in Spain by Ae. albopictus

Índices de conforto térmico desenvolvidos para búfalas criadas em clima tropical quente e úmido.

Para desenvolver índices de conforto térmico para bubalinos, foram utilizadas 20 búfalas Murrah, criadas na Amazônia Oriental, durante um ano. Foram mensuradas temperatura do ar (TA), umidade relativa do ar (UR), temperatura de ponto de orvalho (TPO) e temperatura de globo negro (TGN), além das variáveis fisiológicas temperatura retal (TR), frequência respiratória (FR) e temperatura da pele (TP). Foram feitas análises de regressão multivariadas, utilizando-se o modelo de correlações canônicas. Foram determinados dois pares de índices (Geral e Prático), contendo o Indicativo das Condições Climáticas de Conforto de Búfalas (ICCCB) e o Indicativo do Conforto Ambiental para Búfalas (ICAB). A interpretação dos resultados é dada em escalas que indicam conforto, perigo, estresse e emergência. Os índices foram validados e podem ser utilizados, em pares, para diagnóstico de estresse térmico em bubalinos na região amazônica

Efeito da densidade de plantas na incidência de podridão branca em cebola.

O objetivo deste estudo foi avaliar o efeito do adensamento de plantas na incidência de podridão branca em cebola em solo naturalmente contaminado com o patógeno.bitstream/item/201079/1/BPD-183-15-08-2019-1.pd

Performance of intraspecific hybrids (Kouillou x Robusta) of Coffea canephora Pierre.

The experiment was carried out in 2005 in the municipality of Ouro Preto do Oeste at the experimental station of Embrapa Rondônia. 256 genotypes were evaluated, from nine hybridizations among parents of Kouillou (Cpafro 121, Cpafro 194, Cpafro 199 and Emcapa 03) and Robusta (Robusta 1675, Robusta 2258 and Robusta 640). The experimental design was a randomized complete block design, where each cross was represented by a maximum of 32 plants, distributed in four replications. The spacing used was 3.5 x 1.5 m. The average of six harvests and the yield of hulled coffee was 74.90 bags ha-1. Highlight for progeny is Emcapa 03 x Cpafro 194 with average yield of 97.71 bags ha-1. The estimated heritability observed for yield of coffee benefited, was 78.96%. The evaluation of six production seasons was allowed to verify the reliability of the genetic parameters in each set of crops. It was possible to observe that only the evaluation of the 1st and 2nd crops would be enough to estimate the genetic parameters with efficiency, with a coefficient of determination (R2) of 79.10%. With the inclusion of the 3rd crop, R2 estimates rose to 81.10%. The individual evaluations (per plant) carried out in 2006/2007, 2007/2008 and 2008/2009 crops showed the superiority of the plants from crosses 7, 8 and 9. In relation to resistance of orange rust, most of the genotypes were resistant to disease, and 75% of the genotypes did not present any type of infection symptoms. Among the susceptible genotypes, there was a variation between 1 and 15% in the degree of lesion intensity