Synergism between fungal enzymes and bacterial antibiotics may enhance biocontrol

The interactions between biocontrol fungi and bacteria may play a key role in the natural process of biocontrol, although the molecular mechanisms involved are still largely unknown. Synergism can occur when different agents are applied together, and cell wall degrading enzymes (CWDEs) produced by fungi can increase the efficacy of bacteria. Pseudomonas spp. produce membrane-disrupting lipodepsipeptides (LDPs) syringotoxins (SP) and syringomycins (SR). SR are considered responsible for the antimicrobial activity, and SP for the phytotoxicity. CWDEs of Trichoderma spp. synergistically increased the toxicity Of SP25-A or SRE purified from P. syringae against fungal pathogens. For instance, the fungal enzymes made Botrytis cinerea and other phytopathogenic fungi, normally resistant to SP25-A alone, more susceptible to this antibiotic. Pseudomonas produced CWDEs in culture conditions that allow the synthesis of the LDPs. Purified bacterial enzymes and metabolites were also synergistic against fungal pathogens, although this mixture was less powerful than the combination with the Trichoderma CWDEs. The positive interaction between LDPs and CWDEs may be part of the biocontrol mechanism in some Pseudomonas strains, and co-induction of different antifungal compounds in both biocontrol bacteria and fungi may occur

Rupture Process of the 2004 Sumatra–Andaman Earthquake from Tsunami Waveform Inversion

The aim of this work is to infer the slip distribution and rupture velocity along the rupture zone of the 26 December 2004 Sumatra–Andaman earthquake from available tide gage records of the tsunami. We selected waveforms from 14 stations, distributed along the coast of the Indian Ocean. Then we subdivided the fault plane into 16 subfaults (both along strike and downdip) following the geometry and mechanism proposed by Banerjee et al. (2005) and computed the corresponding Green’s functions by numerical solution of the shallow-water equations through a finitedifference method. The slip distribution and rupture velocity were determined simultaneously by means of a simulated annealing technique. We compared the recorded and synthetic waveforms in the time domain, using a cost function that is a trade-off between the L1 and L2 norms. Preliminary tests on a synthetic dataset, together with a posteriori statistical analysis of the model ensemble enabled us to assess the effectiveness of the method and to quantify the model uncertainty. The main finding is that the best source model features a nonuniform distribution of coseismic slip, with high slip values concentrated into three main patches: the first is located in the southern part of the fault, off the coast of the Aceh Province; the second between 6.5 N and 11 N; and the third at depth, between 11 N and 14 N. Furthermore, we estimated that the rupture propagated at an average speed of 2.0 km/sec

Exploring health care professionals’ experiences of supporting LGBTQ+ patients: a qualitative study

BACKGROUND: Patients from the LGBTQ+ community report negative healthcare experiences, such as healthcare professionals (HCPs) making assumptions about their identities. Research shows that HCPs report not having enough knowledge to facilitate an open conversation with patients from the LGBTQ+ community, leading to patients feeling ignored. AIMS: To explore HCPs’ experiences of supporting patients from the LGBTQ+ community. METHOD: Semi-structured interviews were conducted with HCPs recruited from the research teams’ professional network. Data were analysed using deductive thematic analysis. FINDINGS: HCPs reported positive and negative experiences, as well as a variety of barriers and facilitators to effective communication, with patients from the LGBTQ+ community. HCPs discussed how clinical practice could improve, for example, by developing more inclusive training that is specific to the HCPs’ clinical group. CONCLUSIONS: HCP training needs to be more inclusive of LGBTQ+ identities. It should be tailored to the HCPs’ patient group as this better reflects the varied needs of different clinical groups. Chelsea Pearce and Claudio di Lorito explore health care professionals’ experiences of supporting LGBTQ+ patient

Exploring the individual experiences of LGBT+ patients with general practice staff: an interview-based study

In this qualitative study, Sophie Whyman and Claudio Di Lorito explore the experiences of LGBT+ patients in general practice. / Background: General practitioners (GPs) are usually the first port of call for health-related issues. The attitudes and behaviours of staff working in general practice can influence patient outcomes and access to services. / Aims: This study aimed to explore the experiences of LGBT+ patients with staff working in general practice. / Methods: Qualitative interviews were conducted with LGBT+ participants recruited through the York LGBT Forum and social media and analysed using thematic analysis. / Findings: Six participants were included. The participants reported mixed experiences, which had a great impact on their disclosure, treatment outcomes and future service access. Recommended improvements included standardised training and administrative changes. / Conclusion: There is a need for standardised NHS-wide inclusiveness training to support staff in general practice to better understand non-heteronormative experiences in their clinical practice

Ageing in forensic psychiatric secure settings: the views of members of staff

Background: Although the prevalence of older patients in forensic psychiatric services is increasing, research around service provision for this population is very limited. We aimed to gather the views of members of staff on how well secure services are meeting the challenges of an ageing population.Methods: Three focus groups were carried out with 13 members of staff working with older patients in secure services. A topic guide, based on the research team’s previous research, guided the sessions. The focus groups were audio-recorded, transcribed and analysed through thematic analysis.Results: Two themes were identified: (1) Identifying patients’ needs, which focused on how promptly any emerging issues in the older patients are identified and reported; (2) addressing patients’ needs, which focused on how the unique needs of the older patients are addressed, once established.Conclusions: There are unique age-related issues that may have an impact on the older patients’ opportunities for recovery, including a lack of specialist training for members of staff, prolonged stay in secure care and a limited number of age-relevant activities. Far from optimal, provision requires improvement through the active involvement of the primary stakeholders

Ageing in forensic psychiatric secure settings: the voice of older patients

Older patients account for around 20% of the population in secure forensic psychiatric services in the UK. However, little qualitative research has investigated the experience of ageing in secure settings. This study aimed to gather the individual views of a sample of patients over 50 years old in the UK at different levels of security. Fifteen participants were selected and underwent one-on-one qualitative interviews. The interviews were analysed through thematic analysis, which generated seven themes: Self-agency, activities, social life, practical matters, recovery, physical health and service improvement. Study findings highlighted the complexity of ageing in secure settings. Despite the positive feedback reported in aspects such as physical health care, education opportunities, staff and support of religious practices, participants experienced added barriers to recovery, caused by social isolation/withdrawal and activities/treatment that did not respond to their complex age-related needs, generating poor motivation to engage. Our findings call for the development/implementation of programmes tailored to the unique needs of older patients. This process requires an active involvement of the primary stakeholders and further patient-centred research

The characteristics and needs of older forensic psychiatric patients: a cross-sectional study in secure units within one UK regional service

Although the number of older patients in forensic psychiatric settings is increasing, there is limited information around their sociodemographic characteristics, needs and cognitive abilities. This cross-sectional study focuses on patients aged ≥50 years in three (high, medium and low) secure forensic psychiatric services in England. The study comprises: 1. Analysis of sociodemographic characteristics of all patients (n = 94) in the services; 2. Analysis of the clinical notes and administration of needs and cognitive assessments to a subsample of patients (n = 41). The main outcomes include: sociodemographic characteristics, data on residency, risk, violence, mental and physical health, cognitive ability and individual needs. Data analysis is carried out through descriptive tests and correlation and inferential analyses of outcomes. Results evidence that most patients are White-British single males aged 50–54 years and 88% have at least one physical health condition. A quarter of the patients has cognitive impairment. The most common psychiatric disorder is Personality Disorder (60%); comorbid psychiatric disorders are prevalent (54%). Length of stay averages 6+ years and is longest in high security. Patients’ needs are mostly met. The least met needs are social opportunities. Future comparative research against younger populations could give better context to research findings from this study

Rupture Process of the April 18, 1906 California Earthquake from near-field Tsunami Waveform Inversion

The April 18, 1906 M8 California earthquake generated a small local tsunami that was recorded in the near-field by the Presidio, San Francisco tide-gage, located near the Golden Gate. We investigate the causative, tsunamigenic seismic source by forward modeling and nonlinear inversion of the Presidio marigram. We use existing seismological and geological observations to fix the fault system geometry and the surface slip on the onland portions of the San Andreas fault (SAF). We perform synthetic inversions to show that the single, near-field marigram constrains the main features of the rupture on the portion of the SAF system offshore of the Golden Gate. Finally we perform nonlinear inversions for the slip distribution and the timing of the rupture of the 1906 earthquake. Our results, in agreement with previous studies, identify a dilatational step-over and show a bi-lateral rupture, possibly originating or propagated through the step-over region. We find that little or no co-seismic slip on normal faults in the step-over region is required to fit the marigram, and we obtain adequate fits when allowing delays in the source initiation times of up to 3 minutes on the various fault segments. We constrain slip to be of about 5-6 meters for the onshore portion of the SAF to the northwest of the Golden Gate, in agreement with 1906 surface observations of fault offset. Our results favour the hypothesis of a vertical dip for a currently aseismic SAF to the southeast of the Golden Gate, under the San Francisco Peninsula