20 research outputs found
Advances in Video Coding for Broadcast Applications
1Department of Biomedic Engineering, Electronics and Telecommunications, Universita Politecnica delle Marche, Via Brecce Bianche 12, 60131 Ancona, Italy 2Video Broadcasting Coding, Basingstoke, UK 3STMicroelectronics, Research & Innovation, 20041 Milano, Italy 4 Image and Video Processing Group (GPIV), Multimedia Applications and Telecommunications Institute (iTEAM), Technical University of Valencia, Camino de Vera, 46022 Valencia, Spain 5Electrical and Computer Engineering, Indiana University-Purdue University, 723 West Michigan Street, SL160, Indianapolis, IN 46202, US
Assessment of aflatoxin M1 enrichment factor in cheese produced with naturally contaminated milk
Aflatoxin M1 (AFM1) is a well-known carcinogenic compound that may contaminate milk and dairy products. Thus, with the regulation 1881/2006, the European Union established a concentration limit for AFM1 in milk and insisted on the importance of defining enrichment factors (EFs) for cheese. In 2019, the Italian Ministry of Health proposed four different EFs based on cheeseâs moisture content on a fat-free basis (MMFB) for bovine dairy products. This study aimed to define the EFs of cheese with different MFFB. The milk used for cheesemaking was naturally contaminated with different AFM1 concentrations. Results showed that all the EF average values from this study were lower than those of the Italian Ministry of Health. Hence, the current EFs might need to be reconsidered for a better categorization of AFM1 risk in cheese
Analysis and Tools for Improved Management of Connectionless and Connection-Oriented BLE Devices Coexistence
With the introduction of low-power wireless technologies, like Bluetooth Low Energy (BLE), new applications are approaching the home automation, healthcare, fitness, automotive and consumer electronics markets. BLE devices are designed to maximize the battery life, i.e., to run for long time on a single coin-cell battery. In typical application scenarios of home automation and Ambient Assisted Living (AAL), the sensors that monitor relatively unpredictable and rare events should coexist with other sensors that continuously communicate health or environmental parameter measurements. The former usually work in connectionless mode, acting as advertisers, while the latter need a persistent connection, acting as slave nodes. The coexistence of connectionless and connection-oriented networks, that share the same central node, can be required to reduce the number of handling devices, thus keeping the network complexity low and limiting the packet's traffic congestion. In this paper, the medium access management, operated by the central node, has been modeled, focusing on the scheduling procedure in both connectionless and connection-oriented communication. The models have been merged to provide a tool supporting the configuration design of BLE devices, during the network design phase that precedes the real implementation. The results highlight the suitability of the proposed tool: the ability to set the device parameters to allow us to keep a practical discovery latency for event-driven sensors and avoid undesired overlaps between scheduled scanning and connection phases due to bad management performed by the central node
Virulence Factors and Antimicrobial Resistance Profile of Escherichia Coli Isolated from Laying Hens in Italy
Colibacillosis is the most common bacterial disease in the poultry industry. The isolation
of Escherichia coli (E. coli) strains with multiple resistance to various classes of antimicrobials has
been increasing in recent years. In this study, antimicrobial resistance features, serotyping and
the presence of avian pathogenic Escherichia coli (APEC) virulence genes were investigated on a
total of 71 E. coli strains isolated during outbreaks of colibacillosis in laying hens. The correlation
between these features was evaluated. The most frequently isolated serogroups were O2 and O88.
Resistance was often detected with nalidixic acid (49%) and ampicillin (38%), while all strains were
sensitive to ceftiofur and florfenicol. Overall, 25% of the isolates showed resistance to at least three
or more antimicrobial classes (multidrug-resistant strains), and 56% of the isolates were defined
as APEC strains (due to the presence of at least five virulence genes). Correlation between the
different parameters (virulence genes, serogroup and antimicrobial resistance) did not reveal relevant
associations. The comparison of the obtained results with those of similar studies highlighted
the importance of continuous monitoring in order to have a better understanding of colibacillosis.
An evaluation of the national epidemiological situation would allow, especially with regard to
antimicrobial resistance, to focus on the right measures in order to prioritize the available resources
for effective disease control
First Report of Caseous Lymphadenitis by <i>Corynebacterium pseudotubercolosis</i> and Pulmonary Verminosis in a Roe Deer (<i>Capreolus capreolus</i> Linnaeus, 1758) in Italy
Caseous lymphadenitis is a chronic debilitating disease typical of small ruminants, but it is also noted in several other domestic and wild species. In this report, we present the first documented case in Italy of pseudotuberculosis in a roe deer (Capreolus capreolus, Linnaeus 1758) found dead in the mountains of ForlĂŹ-Cesena province, Emilia Romagna region. The carcass underwent necropsy according to standard protocols, revealing generalized lymphadenopathy and severe apostematous pneumonia with multifocal and encapsulated abscesses. Corynebacterium pseudotuberculosis was isolated from the lung parenchyma, lymph nodes and abscesses. Additionally, severe parasitic bronchopneumonia of the caudal lobes and gastrointestinal strongyle infestation were detected. To our knowledge, this is the first documented case of CLA referable to C. pseudotubercolosis in a roe deer in Italy
Prevalence of Different <i>Salmonella enterica</i> Subspecies and Serotypes in Wild Carnivores in Emilia-Romagna Region, Italy
Salmonella is a pathogen of considerable health concern, given its zoonotic potential, and, in Italy, is the most frequently reported causative agent for foodborne outbreaks. Wild animals and in particular wild carnivores may be carriers of different Salmonella enterica subspecies and serotypes. Given their potential role as reservoirs, surveillance activities are necessary. This study aims to investigate the presence of different Salmonella subspecies and serotypes in wild carnivores in the Emilia-Romagna Region. A total of 718 fox (Vulpes vulpes), 182 badger (Meles meles) and 27 wolf (Canis lupus) carcasses, submitted between 2016â2022, were included for the present work. Gender and age data were collected along with geographical coordinates of carcassâ discovery site. Contents of the large intestine were sampled and cultured according to ISO 6579-1 and both serogroup and serotype identification were performed according to ISO/TR 6579-3:2014. Salmonella was retrieved from 42 foxes (6%), 21 badgers (12%) and 3 wolves (12%), respectively. Isolated Salmonella enterica strains belonged to 4 different subspecies and 25 different serotypes. S. veneziana and S. typhimurium were the most frequent serotypes found (11/67 and 10/67, respectively). In conclusion, zoonotic serotypes were found in all these species of wildlife, thus confirming their potential role in the ecology of Salmonella spp
Potential Applications of Essential Oils for Environmental Sanitization and Antimicrobial Treatment of Intensive Livestock Infections
The extensive use of antibiotics has contributed to the current antibiotic resistance crisis. Livestock infections of Salmonella spp, Clostridium spp. and E. coli antimicrobial-resistant bacteria represent a public threat to human and animal health. To reduce the incidence of these zoonoses, essential oils (EOs) could be effective antibiotic alternatives. This study aims at identifying EOs safe for use, effective both in complementary therapy and in the environmental sanitization of intensive farming. Natural products were chemo-characterized by gas chromatography. Three S. Typhimurium, three C. perfringens and four E. coli strains isolated from poultry and swine farms were used to assess the antimicrobial properties of nine EOs and a modified GR-OLI (mGR-OLI). The toxicity of the most effective ones (Cinnamomum zeylanicum, Cz; Origanum vulgare, Ov) was also evaluated on porcine spermatozoa and Galleria mellonella larvae. Cz, Ov and mGR-OLI showed the strongest antimicrobial activity; their volatile components were also able to significantly inhibit the growth of tested strains. In vitro, Ov toxicity was slightly lower than Cz, while it showed no toxicity on G. mellonella larvae. In conclusion, the study confirms the importance of evaluating natural products to consolidate the idea of safe EO applications in reducing and preventing intensive livestock infections
Table_1_Efficacy of live attenuated, vector and immune complex infectious bursal disease virus (IBDV) vaccines in preventing field strain bursa colonization: A European multicentric study.DOCX
Infectious bursal disease virus (IBDV) is among the most relevant and widespread immunosuppressive agents, which can severely damage poultry farming by causing direct losses, predisposing the host to secondary diseases and reducing the efficacy of vaccination protocols against other infections. IBDV has thus been the object of intense control activities, largely based on routine vaccination. However, the need for protecting animals from the infection in the first period of the production cycle, when the bursa susceptibility is higher, clashes with the blanketing effect of maternally derived antibodies. To overcome this issue, other strategies have been developed besides live attenuated vaccines, including vector vaccines and immune complex (icx) ones. The present study aims to investigate, in field conditions, the efficacy of these approaches in preventing IBDV infection in laying chickens vaccinated with either live attenuated, vector or immune complex (icx) vaccines. For this purpose, a multicentric study involving 481 farms located in 11 European countries was organized and IBDV infection diagnosis and strain characterization was performed at 6 weeks of age using a molecular approach. Vaccine strains were commonly detected in flocks vaccinated with live or icx vaccines. However, a significantly higher number of field strains (characterized as very virulent IBDVs) was detected in flocks vaccinated with vector vaccines, suggesting their lower capability of preventing bursal colonization. Different from vector vaccines, live and icx ones have a marked bursal tropism. It can thus be speculated that vaccine virus replication in these sites could limit vvIBDV replication by direct competition or because of a more effective activation of innate immunity. Although such different behavior doesn't necessarily affect clinical protection, further studies should be performed to evaluate if vvIBDV replication could still be associated with subclinical losses and/or for viral circulation in a âvaccinated environmentâ could drive viral evolution and favor the emergence of vaccine-escape variants.</p
Interoperability issues among smart home technological rameworks
Population aging may be seen both as a human success story, the triumph of public health, medical advancements and economic development over diseases and injures, and as one of the most challenging phenomena that society faces in this century. Assistive technology in all its possible implementations (from Telemedicine to Ambient Assisted Living, and Ambient Intelligence) represents an emerging answer to the needs of the new generation of older adults whose desire is to live longer with a higher quality of life. Objective of this paper is to present the results of a public financed action for the development and implementation of an âintegration platformâ for Ambient Assisted Living that includes features of home automation (energy management, safety, comfort, etc.) and introduces âsmart objectsâ, to monitor activities of daily living and detect any abnormal behavior that may represent a danger, or highlight symptoms of some incipient disease
Telecommunications in the ICT Age: From Research to Applications
The human society in the information age deeply relies on digital information processing, communication and storage. Photonic routing and switchingis expected to be exploited in future all-optical networks. Channel coding is needed in order to protect information against natural disturbances, and modern coding schemes are able to reach the ultimate limits predicted by Shannon. On the other hand, postquantum cryptography is necessary for assuring security against cyber attackers, possibly provided with quantum computers. Source coding, especially in video data compression, is recommended for optimizing the bandwidth usage. Spread spectrum systems can solve the problem of radio transmissions over common frequency bands. These technologies are of crucial importance for the evolution of networks and of the whole Internet, allowing people to interact each other and access information in the web. Nowadays, the conventional Internet of people has moved into the pervasive Internet of Things providing innovative services in a variety of application fields. In this respect, domotic systems, based on ambient and wearable sensors, appear of dramatic importance in the design of future assisted living protocols