Letter to Sonora Dodd from Robert H. Lord, June 20, 1911

Letter to Sonora Dodd from Robert H. Lord, with envelope and business card. Robert Lord was The Shop-Keeper of Lord\u27s Little Shop, Springfield, Illinois.https://digitalcommons.whitworth.edu/fathers-day-correspondence/1002/thumbnail.jp

Folding-competent and folding-defective forms of Ricin A chain have different fates following retrotranslocation from the endoplasmic reticulum

We report that a toxic polypeptide retaining the potential to refold upon dislocation from the endoplasmic reticulum (ER) to the cytosol (ricin A chain; RTA) and a misfolded version that cannot (termed RTAΔ), follow ER-associated degradation (ERAD) pathways in Saccharomyces cerevisiae that substantially diverge in the cytosol. Both polypeptides are dislocated in a step mediated by the transmembrane Hrd1p ubiquitin ligase complex and subsequently degraded. Canonical polyubiquitylation is not a prerequisite for this interaction because a catalytically inactive Hrd1p E3 ubiquitin ligase retains the ability to retrotranslocate RTA, and variants lacking one or both endogenous lysyl residues also require the Hrd1p complex. In the case of native RTA, we established that dislocation also depends on other components of the classical ERAD-L pathway as well as an ongoing ER–Golgi transport. However, the dislocation pathways deviate strikingly upon entry into the cytosol. Here, the CDC48 complex is required only for RTAΔ, although the involvement of individual ATPases (Rpt proteins) in the 19S regulatory particle (RP) of the proteasome, and the 20S catalytic chamber itself, is very different for the two RTA variants. We conclude that cytosolic ERAD components, particularly the proteasome RP, can discriminate between structural features of the same substrate

U.S. GLOBEC Georges Bank long-term moored program : part 1 - mooring configuration

As part of the U.S. GLOBEC Northwest Atlantic/Georges Bank program, moorings were deployed on Georges Bank as part of the broad-scale survey component to help measure the temporal variability of both physical and biological characteristics on the Bank. The array consisted of a primary mooring site on the Southern Flank which was maintained for the full 5-year duration of the field program, plus secondary moorings, with fewer sensors and of shorter duration, in the well-mixed water on the Crest and in the cod/haddock spawning region on the Northeast Peak. Temperature and conductivity (salinity) were measured at 5-m intervals, ADCP velocity profiles were obtained with 1-m vertical resolution, and bio-optical packages (measuring fluorescence, optical transmission and photosynthetically active radiation) were deployed at 10-m and 40-m depths. Bottom pressure was measured at the Southern Flank site. The buoy design, sensors and mooring configuration is presented and discussed below, and the data obtained is presented and discussed in an accompanying reports “U.S. GLOBEC Georges Bank Long-Term Moored Program: Part 2 – Yearly Data Summary and Report,” and “U.S. GLOBEC Georges Bank Long-Term Moored Program: Part 3 – Data Summary.”Funding was provided by the National Science Foundation under grant numbers OCE-93-13670, OCE-96-32348, OCE98-06379, OCE-98-06445 and OCE-02-27679

CLIVAR Mode Water Dynamics Experiment (CLIMODE) fall 2006 R/V Oceanus voyage 434 November 16, 2006–December 3, 2006

CLIMODE (CLIVAR Mode Water Dynamic Experiment) is a research program designed to understand and quantify the processes responsible for the formation and dissipation of North Atlantic subtropical mode water, also called Eighteen Degree Water (EDW). Among these processes, the amount of buoyancy loss at the ocean-atmosphere interface is still uncertain and needs to be accurately quantified. In November 2006, cruise 434 onboard R/V Oceanus traveled in the region of the separated Gulf Stream and its recirculation, where intense oceanic heat loss to the atmosphere in the winter is believed to trigger the formation of EDW. During this cruise, the surface mooring F that was anchored in the core of the Gulf Stream was replaced by a new one, as well as two subsurface moorings C and D located on the southeastern edge of the stream. Surface drifters, ARGO and bobbers RAFOS floats were deployed, CTD profiles and water samples were also carried out. This array of instruments will permit a characterization of EDW with high spatial and temporal resolutions and accurate in-situ measurements of air-sea fluxes in the EDW formation region. The present report documents this cruise, the methods and locations for the deployments of instruments and some evaluation of the measurements from these instruments.Funding was provided by the National Science Foundation under contract No. OCE04-2453

Iatrogenic Spinal Cord Injury Resulting From Cervical Spine Surgery.

STUDY DESIGN: Retrospective cohort study of prospectively collected data. OBJECTIVE: To examine the incidence of iatrogenic spinal cord injury following elective cervical spine surgery. METHODS: A retrospective multicenter case series study involving 21 high-volume surgical centers from the AOSpine North America Clinical Research Network was conducted. Medical records for 17 625 patients who received cervical spine surgery (levels from C2 to C7) between January 1, 2005, and December 31, 2011, were reviewed to identify occurrence of iatrogenic spinal cord injury. RESULTS: In total, 3 cases of iatrogenic spinal cord injury following cervical spine surgery were identified. Institutional incidence rates ranged from 0.0% to 0.24%. Of the 3 patients with quadriplegia, one underwent anterior-only surgery with 2-level cervical corpectomy, one underwent anterior surgery with corpectomy in addition to posterior surgery, and one underwent posterior decompression and fusion surgery alone. One patient had complete neurologic recovery, one partially recovered, and one did not recover motor function. CONCLUSION: Iatrogenic spinal cord injury following cervical spine surgery is a rare and devastating adverse event. No standard protocol exists that can guarantee prevention of this complication, and there is a lack of consensus regarding evaluation and treatment when it does occur. Emergent imaging with magnetic resonance imaging or computed tomography myelography to evaluate for compressive etiology or malpositioned instrumentation and avoidance of hypotension should be performed in cases of intraoperative and postoperative spinal cord injury

Training of Instrumentalists and Development of New Technologies on SOFIA

This white paper is submitted to the Astronomy and Astrophysics 2010 Decadal Survey (Astro2010)1 Committee on the State of the Profession to emphasize the potential of the Stratospheric Observatory for Infrared Astronomy (SOFIA) to contribute to the training of instrumentalists and observers, and to related technology developments. This potential goes beyond the primary mission of SOFIA, which is to carry out unique, high priority astronomical research. SOFIA is a Boeing 747SP aircraft with a 2.5 meter telescope. It will enable astronomical observations anywhere, any time, and at most wavelengths between 0.3 microns and 1.6 mm not accessible from ground-based observatories. These attributes, accruing from the mobility and flight altitude of SOFIA, guarantee a wealth of scientific return. Its instrument teams (nine in the first generation) and guest investigators will do suborbital astronomy in a shirt-sleeve environment. The project will invest $10M per year in science instrument development over a lifetime of 20 years. This, frequent flight opportunities, and operation that enables rapid changes of science instruments and hands-on in-flight access to the instruments, assure a unique and extensive potential - both for training young instrumentalists and for encouraging and deploying nascent technologies. Novel instruments covering optical, infrared, and submillimeter bands can be developed for and tested on SOFIA by their developers (including apprentices) for their own observations and for those of guest observers, to validate technologies and maximize observational effectiveness.Comment: 10 pages, no figures, White Paper for Astro 2010 Survey Committee on State of the Professio

The Current State of Performance Appraisal Research and Practice: Concerns, Directions, and Implications

On the surface, it is not readily apparent how some performance appraisal research issues inform performance appraisal practice. Because performance appraisal is an applied topic, it is useful to periodically consider the current state of performance research and its relation to performance appraisal practice. This review examines the performance appraisal literature published in both academic and practitioner outlets between 1985 and 1990, briefly discusses the current state of performance appraisal practice, highlights the juxtaposition of research and practice, and suggests directions for further research

ISO--LWS observations of the two nearby spiral galaxies: NGC6946 and NGC1313

(Abridged) We present the analysis of the main FIR fine structure lines emission in NGC1313 and NGC6946. We calculate that a component probably associated with the diffuse disks contributes <~40% in N6946 and ~30 % in N1313 to the total [CII] emission. The main PDR physical parameters responsible for the neutral atomic gas emission in N1313 and N6946 do not significantly differ from what Malhotra etal (2001) found by modelling the integrated emission of a sample of 60 normal galaxies,although there are evidences for a beam averaged contribution of a less active component inside NGC6946 higher than its contribution in the integrated emission of normal galaxies. CO and [CII] in N6946 are well correlated with a mean [CII]/CO ratio similar to that of the normal galaxies sample. In N1313 the [CII]/CO seems to systematically increase from the North to the south, along the S-shaped spiral arm, indicating much more inhomogeneous conditions than in N6946. HI and [CII] in N6946 are completely de-correlated, probably because they arise from different gas components. In N1313 we successfully detect two distinct gas components: a cirrus-like component where HI and [CII] are weakly correlated as observed in our Galaxy, and a component associated with dense PDRs completely de-correlated from HI as observed in N6946.Finally, we find that the HI residing in dense PDRs and presumably recently photo-dissociated, constitutes a few % of the total HI. In turn, this dense gas component produces most of the [CII] emission emitted by the atomic neutral medium.Comment: Latex, 100 pages, 11 Figures, 11 Tables. Accepted for publication in A

A case of autism with an interstitial deletion on 4q leading to hemizygosity for genes encoding for glutamine and glycine neurotransmitter receptor sub-units (AMPA 2, GLRA3, GLRB) and neuropeptide receptors NPY1R, NPY5R

BACKGROUND: Autism is a pervasive developmental disorder characterized by a triad of deficits: qualitative impairments in social interactions, communication deficits, and repetitive and stereotyped patterns of behavior. Although autism is etiologically heterogeneous, family and twin studies have established a definite genetic basis. The inheritance of idiopathic autism is presumed to be complex, with many genes involved; environmental factors are also possibly contributory. The analysis of chromosome abnormalities associated with autism contributes greatly to the identification of autism candidate genes. CASE PRESENTATION: We describe a child with autistic disorder and an interstitial deletion on chromosome 4q. This child first presented at 12 months of age with developmental delay and minor dysmorphic features. At 4 years of age a diagnosis of Pervasive Developmental Disorder was made. At 11 years of age he met diagnostic criteria for autism. Cytogenetic studies revealed a chromosome 4q deletion. The karyotype was 46, XY del 4 (q31.3-q33). Here we report the clinical phenotype of the child and the molecular characterization of the deletion using molecular cytogenetic techniques and analysis of polymorphic markers. These studies revealed a 19 megabase deletion spanning 4q32 to 4q34. Analysis of existing polymorphic markers and new markers developed in this study revealed that the deletion arose on a paternally derived chromosome. To date 33 genes of known or inferred function are deleted as a consequence of the deletion. Among these are the AMPA 2 gene that encodes the glutamate receptor GluR2 sub-unit, GLRA3 and GLRB genes that encode glycine receptor subunits and neuropeptide Y receptor genes NPY1R and NPY5R. CONCLUSIONS: The deletion in this autistic subject serves to highlight specific autism candidate genes. He is hemizygous for AMPA 2, GLRA3, GLRB, NPY1R and NPY5R. GluR2 is the major determinant of AMPA receptor structure. Glutamate receptors maintain structural and functional plasticity of synapses. Neuropeptide Y and its receptors NPY1R and NPY5R play a role in hippocampal learning and memory. Glycine receptors are expressed in very early cortical development. Molecular cytogenetic studies and DNA sequence analysis in other patients with autism will be necessary to confirm that these genes are involved in autism

Discriminatory optical force for chiral molecules

We suggest that the force F exerted upon a chiral molecule by light assumes the form F = a∇w + b∇h under appropriate circumstances, where a and b pertain to the molecule whilst w and h are the local densities of electric energy and helicity in the optical field; the gradients ∇ of these quantities thus governing the molecule’s centre-of-mass motion. Whereas a is identical for the mirror- image forms or enantiomers of the molecule, b has opposite signs; the associated contribution to F therefore pointing in opposite directions. A simple optical field is presented for which ∇w vanishes but ∇h does not, so that F is absolutely discriminatory. We then present two potential applications: a Stern–Gerlach-type deflector capable of spatially separating the enantiomers of a chiral molecule and a diffraction grating to which chiral molecules alone are sensitive; the resulting diffraction patterns thus encoding information about their chiral geometry