9,160 research outputs found
Forward: The Reagan Legacy and the Strategic Defense Initiative;The Reagan Legacy and the Strategic Defense Initiative: Foreword
Forward: The Reagan Legacy and the Strategic Defense Initiative;The Reagan Legacy and the Strategic Defense Initiative: Foreword
Genomic analysis of eight native plasmids of the phytopathogen Pseudomonas syringae
ComunicaciĂłn a conferenciaThe pPT23A family of plasmids (PFPs) appears to be indigenous to the plant pathogen Pseudomonas syringae and these plasmids are widely distributed and widely transferred among pathovars of P. syringae and related species. PFPs are sources of accessory genes for their hosts that can include genes important for virulence and epiphytic colonization of plant leaf surfaces. Further understanding of the evolution of the pPT23A plasmid family and the role of these plasmids in P. syringae biology and pathogenesis, requires the determination and analysis of additional complete, closed plasmid genome sequences. Therefore, our main objective was to obtain complete genome sequences from PFPs from three different P. syringae pathovars and perform a comparative genomic analysis. In this work plasmid DNA isolation, purification by CsCl-EtBr gradients, and sequencing using 454 platform, were used to obtain the complete sequence of P. syringae plasmids. Different bioinformatic tools were used to analyze the plasmid synteny, to identify virulence genes (i.e. type 3 effectors) and to unravel the evolutionary history of PFPs. Our sequence analysis revealed that PFPs from P. syringae encode suites of accessory genes that are selected at different levels (universal, interpathovar and intrapathovar). The conservation of type IVSS encoding conjugation functions also contributes to the distribution of these plasmids within P. syringae populations. Thus, this study contributes to unravel the genetic basis of the role of PFPs in different P. syringae lifestyles.Universidad de Málaga. Campus de Excelencia Internacional AndalucĂa Tech
Comparative genomic analysis of native pseudomonas syringae plasmids belonging to the ppt23 a family reveals their role in p. Syringae epiphytic and pathogenic lifestyles
Backgrounds
The pPT23A family of plasmids (PFPs) appears to be indigenous to the plant pathogen Pseudomonas syringae and these plasmids are widely distributed and widely transferred among pathovars of P. syringae and related species. PFPs are sources of accessory genes for their hosts that can include genes important for virulence and epiphytic colonization of plant leaf surfaces.
Objectives
Further understanding of the evolution of the pPT23A plasmid family and the role of these plasmids in P. syringae biology and pathogenesis, requires the determination and analysis of additional complete, closed plasmid genome sequences. Therefore, our main objective was to obtain complete genome sequences of PFPs from three different P. syringae pathovars and perform a comprehensive comparative genomic analysis.
Methods
In this work plasmid DNA isolation, purification by CsCl-EtBr gradients, and sequencing using 454 platform, were carried out to obtain the complete sequence of P. syringae plasmids. Different bioinformatic tools were used to analyze the plasmid synteny, to identify virulence genes (i.e. type 3 effectors) and to unravel the evolutionary history of PFPs.
Conclusions
Our sequence analysis revealed that PFPs from P. syringae encode suites of accessory genes that are selected at different levels (universal, interpathovar and intrapathovar). The conservation of type IVSS encoding conjugation functions also contributes to the distribution of these plasmids within P. syringae populations. Thus, this study contributes to unravel the genetic bases of the role of PFPs in different P. syringae lifestyles.
This work was supported by grants Proyecto de Excelencia, Junta de AndalucĂa (P07-AGR-02471; P12-AGR-1473) and by Michigan State University AgBioResearch.This work was supported by grants Proyecto de Excelencia, Junta de AndalucĂa (P07-AGR-02471; P12-AGR-1473) and by Michigan State University AgBioResearch; Universidad de Málaga. Campus de Excelencia Internacional AndalucĂa Tech
Large-Scale Correlations in the Lyman-alpha Forest at z = 3-4
We present a study of the spatial coherence of the intergalactic medium
toward two pairs of high-redshift quasars with moderate angular separations
observed with Keck/ESI, Q1422+2309A/Q1424+2255 (z_em = 3.63, theta = 39") and
Q1439-0034A/B (z_em = 4.25, theta = 33"). The crosscorrelation of transmitted
flux in the Lyman-alpha forest shows a 5-7 sigma peak at zero velocity lag for
both pairs. This strongly suggests that at least some of the absorbing
structures span the 230-300/h_70 proper kpc transverse separation between
sightlines. We also statistically examine the similarity between paired spectra
as a function of transmitted flux, a measure which may be useful for comparison
with numerical simulations. In investigating the dependence of the correlation
functions on spectral characteristics, we find that photon noise has little
impact for S/N >~ 10 per resolution element. However, the agreement between the
autocorrelation along the line sight and the crosscorrelation between
sightlines, a potential test of cosmological geometry, depends significantly on
instrumental resolution. Finally, we present an inventory of metal lines. These
include a a pair of strong C IV systems at z ~ 3.4 appearing only toward
Q1439B, and a Mg II + Fe II system present toward Q1439 A and B at z = 1.68.Comment: 33 pages, 13 figures, submitted to Ap
Application of OCT to examination of easel paintings
We present results of applying low coherence interferometry to gallery paintings. Infrared low coherence interferometry is capable of non-destructive examination of paintings in 3D, which shows not only the structure of the varnish layer but also the paint layers
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