Crayfish Harvesting Practices in the Southern Atchafalaya River Basin: Quantitative and Qualitative Assessment of Harvester Techniques and Hydrologic Connectivity Influence on Harvesting Strategies

Fisheries produce important impacts around the world through the exploitation of a wide range of species. In Louisiana, crayfish is the most emblematic crustacean and supports a multi-million industry based on pond culture and harvest from natural habitats. Although the economic value (USD) of wild-harvested crayfish has decreased from 10% to 3% of total crayfish value from 2013 to 2015, wild harvested crayfish are highly desired by many consumers and have a strong socio-cultural importance in Louisiana and other Gulf of Mexico coastal regions. This project evaluated harvesting practices by: 1) field observation and mapping of harvest sites in southwestern Atchafalaya River Basin; and 2) directed interviews with individual harvesters. Field observations included trap locations, water quality, habitat components, and fishery independent sampling. Weekly field observations were conducted along four transects across a gradient of water quality over two harvesting seasons (2015 and 2016). I also assessed floodplain connectivity with river water sources by conservative tracers sampled at each water quality site biweekly during 2016. Directed interviews of 23 harvesters provided data on fishing strategies, factors used to decide when to start fishing, and selection of harvesting locations. Trap density was first assessed for spatial autocorrelation by Pearson Chi-Square Quadrat Test and Nearest Neighbor Tests and then by generalized linear models including water quality, habitat, harvester answers and conservative tracers. Analyses demonstrated that trap locations were not random, i.e., traps were set in relatively clear water (NTU \u3c 69.4) in in depths from 1-3 m or 3-3.6 m. Very few traps were set high turbidity water regardless of depth. Trap density was positively associated with river water inputs, based on conservative tracer results. Harvester interviews corroborated the importance of tradition (35% - 47%) and depth (88%) when starting harvesting and setting traps. Additionally, harvesters (\u3e 40%) considered water color (likely a surrogate for turbidity) important for trap locations. Although harvesters may not be using water vii quality and chemistry data, their harvesting practices do follow water movements, likely based on accumulated experience with depth, flow velocity and turbidity

The blood labyrinthine barrier in the human normal and Meniere's disease macula utricle.

The ultrastructural organization of the blood labyrinthine barrier (BLB) was investigated in the human vestibular endorgan, the utricular macula, using postmortem specimens from individuals with documented normal auditory and vestibular function and surgical specimens from patients with intractable Meniere's disease. Transmission electron microscopic analysis of capillaries located in the normal human utricular stroma showed vascular endothelial cells with few pinocytotic vesicles, covered by a smooth and uniform basement membrane surrounded by pericyte processes. Meniere's disease specimens revealed differential ultrastructural pathological changes in the cellular elements of the microvasculature. With moderate degeneration of the BLB, there were numerous vesicles within the vascular endothelial cells (VECs), with increased numbers at the abluminal face, pericyte process detachment and disruption of the perivascular basement membrane surrounding the VECs. With severe degeneration of the BLB, there was severe vacuolization or frank apparent necrosis of VECs and loss of subcellular organelles. A higher severity of BLB degenerative changes was associated with a higher degree of basement membrane thickening and edematous changes within the vestibular stroma. This study presents the first ultrastructural analysis of the capillaries constituting the BLB in the human vestibular macula utricle from normal and Meniere's disease

Evidence for oxidative stress in the developing cerebellum of the rat after chronic mild carbon monoxide exposure (0.0025% in air)

<p>Abstract</p> <p>Background</p> <p>The present study was designed to test the hypothesis that chronic very mild prenatal carbon monoxide (CO) exposure (25 parts per million) subverts the normal development of the rat cerebellar cortex. Studies at this chronic low CO exposure over the earliest periods of mammalian development have not been performed to date. Pregnant rats were exposed chronically to CO from gestational day E5 to E20. In the postnatal period, rat pups were grouped as follows: Group A: prenatal exposure to CO only; group B: prenatal exposure to CO then exposed to CO from postnatal day 5 (P5) to P20; group C: postnatal exposure only, from P5 to P20, and group D, controls (air without CO). At P20, immunocytochemical analyses of oxidative stress markers, and structural and functional proteins were assessed in the cerebellar cortex of the four groups. Quantitative real time PCR assays were performed for inducible (iNOS), neuronal (nNOS), and endothelial (eNOS) nitric oxide synthases.</p> <p>Results</p> <p>Superoxide dismutase-1 (SOD1), SOD2, and hemeoxygenase-1 (HO-1) immunoreactivity increased in cells of the cerebellar cortex of CO-exposed pups. INOS and nitrotyrosine immunoreactivity also increased in blood vessels and Purkinje cells (PCs) of pups from group-A, B and C. By contrast, nNOS immunoreactivity decreased in PCs from group-B. Endothelial NOS immunoreactivity showed no changes in any CO-exposed group. The mRNA levels for iNOS were significantly up-regulated in the cerebellum of rats from group B; however, mRNA levels for nNOS and eNOS remained relatively unchanged in groups A, B and C. Ferritin-H immunoreactivity increased in group-B. Immunocytochemistry for neurofilaments (structural protein), synapsin-1 (functional protein), and glutamic acid decarboxylase (the enzyme responsible for the synthesis of the inhibitory neurotransmitter GABA), were decreased in groups A and B. Immunoreactivity for two calcium binding proteins, parvalbumin and calbindin, remained unchanged. The immunoreactivity of the astrocytic marker GFAP increased after prenatal exposure.</p> <p>Conclusion</p> <p>We conclude that exogenously supplied CO during the prenatal period promotes oxidative stress as indicated by the up-regulation of SOD-1, SOD-2, HO-1, Ferritin-H, and iNOS with increased nitrotyrosine in the rat cerebella suggesting that deleterious and protective mechanisms were activated. These changes correlate with reductions of proteins important to cerebellar function: pre-synaptic terminals proteins (synapsin-1), proteins for the maintenance of neuronal size, shape and axonal quality (neurofilaments) and protein involved in GABAergic neurotransmission (GAD). Increased GFAP immunoreactivity after prenatal CO-exposure suggests a glial mediated response to the constant presence of CO. There were differential responses to prenatal vs. postnatal CO exposure: Prenatal exposure seems to be more damaging; a feature exemplified by the persistence of markers indicating oxidative stress in pups at P20, following prenatal only CO-exposure. The continuation of this cellular environment up to day 20 after CO exposure suggests the condition is chronic. Postnatal exposure without prenatal exposure shows the least impact, whereas prenatal followed by postnatal exposure exhibits the most pronounced outcome among the groups.</p

Enhancing M Currents: A Way Out for Neuropathic Pain?

Almost three decades ago, the M current was identified and characterized in frog sympathetic neurons (Brown and Adams, 1980). The years following this discovery have seen a huge progress in the understanding of the function and the pharmacology of this current as well as on the structure of the underlying ion channels. Therapies for a number of syndromes involving abnormal levels of excitability in neurons are benefiting from research on M currents. At present, the potential of M current openers as analgesics for neuropathic pain is under discussion. Here we offer a critical view of existing data on the involvement of M currents in pain processing. We believe that enhancement of M currents at the site of injury may become a powerful strategy to alleviate pain in some peripheral neuropathies

Data-based system representations from irregularly measured data

Non-parametric representations of dynamical systems based on the image of a Hankel matrix of data are extensively used for data-driven control. However, if samples of data are missing, obtaining such representations becomes a difficult task. By exploiting the kernel structure of Hankel matrices of irregularly measured data generated by a linear time-invariant system, we provide computational methods for which any complete finite-length behavior of the system can be obtained. For the special case of periodically missing outputs, we provide conditions on the input such that the former result is guaranteed. We illustrate with an example how the resulting representation provides a more computationally efficient method for low-rank matrix completion when compared to an alternative method.Comment: submitted to IEEE Transactions on Automatic Contro

Identification from data with periodically missing output samples

The identification problem in case of data with missing values is challenging and currently not fully understood. For example, there are no general nonconservative identifiability results, nor provably correct data efficient methods. In this paper, we consider a special case of periodically missing output samples, where all but one output sample per period may be missing. The novel idea is to use a lifting operation that converts the original problem with missing data into an equivalent standard identification problem. The key step is the inverse transformation from the lifted to the original system, which requires computation of a matrix root. The well-posedness of the inverse transformation depends on the eigenvalues of the system. Under an assumption on the eigenvalues, which is not verifiable from the data, and a persistency of excitation-type assumption on the data, the method based on lifting recovers the data-generating system. (Preprint submitted to Automatica)European Research Council (ERC)/European Union’s Horizon 2020 research and innovation programme/948679/E

Molecular dynamics simulation of the regrowth of nanometric multigate Si devices

We use molecular dynamics (MD) simulation techniques to study the regrowth of nanometric multigate Si devices, such as fins and nanowires, surrounded by free surfaces and interfaces with amorphous material. Our results indicate that atoms in amorphous regions close to lateral free surfaces or interfaces rearrange at a slower rate compared to those in bulk due to the discontinuity of the lateral crystalline template. Consequently, the recrystallization front which advances faster in the device center than at the interfaces adopts new orientations. Regrowth then proceeds depending on the particular orientation of the new amorphous/crystal interfaces. In the particular case of (110) oriented fins, the new amorphous/crystal interfaces are aligned along the (111) direction, which produces frequent twining during further regrowth. Based on our simulation results, we propose alternatives to overcome this defected recrystallization in multigate structures: device orientation along (100) to prevent the formation of limiting {111} I amorphous/crystal interfaces and presence of a crystalline seed along the device body to favor regrowth perpendicular to the lateral surfaces/interfaces rather than parallel to them. (C) 2012 American Institute of Physics. [doi :10.1063/1.3679126

Addressing stability issues in mediated complex contract negotiations for constraint-based, non-monotonic utility spaces

Negotiating contracts with multiple interdependent issues may yield non- monotonic, highly uncorrelated preference spaces for the participating agents. These scenarios are specially challenging because the complexity of the agents’ utility functions makes traditional negotiation mechanisms not applicable. There is a number of recent research lines addressing complex negotiations in uncorrelated utility spaces. However, most of them focus on overcoming the problems imposed by the complexity of the scenario, without analyzing the potential consequences of the strategic behavior of the negotiating agents in the models they propose. Analyzing the dynamics of the negotiation process when agents with different strategies interact is necessary to apply these models to real, competitive environments. Specially problematic are high price of anarchy situations, which imply that individual rationality drives the agents towards strategies which yield low individual and social welfares. In scenarios involving highly uncorrelated utility spaces, “low social welfare” usually means that the negotiations fail, and therefore high price of anarchy situations should be avoided in the negotiation mechanisms. In our previous work, we proposed an auction-based negotiation model designed for negotiations about complex contracts when highly uncorrelated, constraint-based utility spaces are involved. This paper performs a strategy analysis of this model, revealing that the approach raises stability concerns, leading to situations with a high (or even infinite) price of anarchy. In addition, a set of techniques to solve this problem are proposed, and an experimental evaluation is performed to validate the adequacy of the proposed approaches to improve the strategic stability of the negotiation process. Finally, incentive-compatibility of the model is studied.Spain. Ministerio de Educación y Ciencia (grant TIN2008-06739-C04-04

Thermal Dose Inactivation of Escherichia coli by Magnetic Induced Hyperthermia

Background: Apoptosis of mutated cells via magnetic hyperthermia has gained advocacy as technology capable of being used in lieu of chemotherapy for targeting cancer tumors. Progress of nanotechnology offers effective remote heating of magnetic fluid via hyperthermia. The heating and specific power absorption of these nanoparticles use in the magnetic fluid are dependent on particle properties and treatment locations. Methods: Nanoparticles were fabricated using microfluidic system by interaction of two solutions containing 2Fe(NO3)3+FeSO4 and NaOH+2%Dextran to create nanostructured media with a biocompatible dextran coating and a Fe3O4 core. The nanoparticles, of a concentration of 5mg/ml, were placed in a vile containing Luria-Bertani (LB) media with approximately 2.0x108 cells. The vile was inserted into a DM100 Series Magnetic Hyperthermia Device that provides an alternating magnetic field of 300 Gauss with a frequency of 604KHz. Results: Magnetite produced via the microfluidic systems at flow rate of 0.04mL/s showed uniform particle size distribution with average size 10nm and saturation magnetization up to 60emu/g as well as pure-phase of Fe3O4 with high crystallinity. Zero-Field-Cooled and Field-Cooled measurements indicated a superparamagnetic nature of as synthesized particles with a low blocking temperature that varies by the amount of dextran introduced in the mixture. Conclusions: The superparamagnetic nanoparticles were heated up to 60°C, inciting a heat shock effect that led to the destruction of the E.coli bacteria. The specific power absorption value obtained was 130 W/g, showing that magnetite–dextran nanostructured fluid appears to be a promising active media for the local magnetic hyperthermia for cancer therapy