Micro e nanoencapsula??o de ?leo de semente de rom? para aplica??o em embalagens ativas

O presente trabalho foi realizado com apoio da Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - Brasil (CAPES) - C?digo de Financiamento 001.O ?leo de semente de rom?, obtido a partir da prensagem a frio das sementes da rom?zeira (Punica granatum L.), vem ganhando aten??o devido aos seus efeitos ben?ficos ? sa?de associados ? sua composi??o rica em compostos antioxidantes. No entanto a utiliza??o desse ?leo ? limitada por sua baixa solubilidade aquosa e elevada possibilidade de degrada??o oxidativa, limita??es que podem ser contornadas atrav?s da sua encapsula??o. A busca por solu??es para melhorar a qualidade dos alimentos, reduzir o uso de aditivos sint?ticos e diminuir os impactos ambientais ? uma demanda crescente dos consumidores nos dias atuais. Uma alternativa interessante para a resolu??o desses problemas s?o as embalagens ativas provenientes de fontes naturais e renov?veis. O objetivo desse trabalho foi a produ??o, caracteriza??o e compara??o entre a utiliza??o de micro e nanopart?culas lip?dicas no desenvolvimento de embalagens biodegrad?veis ativas. As nanopart?culas foram produzidas pelo m?todo de homogeneiza??o a quente seguido por sonica??o, j? para obten??o das micropart?culas o m?todo de homogeneiza??o a quente foi associado a agita??o por rotor-estator. As formula??es de nanopart?culas apresentaram di?metro m?dio na faixa de 308 a 325 nm, enquanto as micropart?culas apresentaram di?metros na faixa de 9,45 a 10,84 ?m, demostrando efic?cia dos m?todos de obten??o das part?culas. Os valores de ?ndice de polidispers?o das nanopart?culas indicam formula??es homog?neas e monodispersas. Os altos valores de potencial zeta negativo observados indicam estabilidade da dispers?o coloidal. As an?lises de difra??o de raios X e espectroscopia no infravermelho evidenciaram a compatibilidade entre os componentes das amostras. Os valores de efici?ncia de encapsula??o obtidos variaram de 40,0 a 66,1%, indicando encapsula??o satisfat?ria do ?leo na matriz. O processo de encapsula??o promoveu a prote??o da atividade antioxidante do ?leo, visto que foi observada diminui??o da porcentagem de inibi??o de DPPH de 55,4% para valores na faixa de 41,4 a 51,3% pela incorpora??o em carreadores lip?dicos. A adi??o de part?culas ? matriz de amido de mandioca gerou filmes mais densos e sol?veis que o filme controle. No geral, as amostras apresentaram superf?cie homog?nea e propriedades mec?nicas superiores ?s do filme controle. A capacidade de inibi??o do radical oxidante dos filmes (16,7 a 20,4%) indica que as part?culas foram capazes de promover maior prote??o dos compostos ativos do ?leo durante o processo de produ??o e acondicionamento dos filmes, o que propicia sua aplica??o para uma variedade de produtos sens?veis a oxida??o.Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES)Disserta??o (Mestrado) ? Programa de P?s-gradua??o em Ci?ncia e Tecnologia de Alimentos, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2022.Pomegranate seed oil, obtained from the cold pressing of pomegranate seeds (Punica granatum L.), has been gaining attention due to its beneficial health effects, associated with its composition rich in antioxidant compounds. The use of this oil is limited by its low aqueous solubility and the high possibility of oxidative degradation, limitations that can be overcome through encapsulation. There is a growing consumer demand for active packaging from natural and renewable sources, as alternatives to improve food quality, use fewer synthetic additives and bring less environmental impacts. The objective of this study was the production, characterization and comparison between the use of lipid micro and nanoparticles for the development of biodegradable active packaging. The nanoparticles were produced by the hot homogenization method followed by sonication and the first method was associated with rotor-stator agitation to obtain microparticles. The nanoparticle formulations showed an average diameter in the range from 308 to 325 nm, while the microparticles presented diameters in the range from 9.45 to 10.84 ?m, demonstrating the effectiveness of the methods for obtaining the particles. The polydispersity index values of the nanoparticles indicate homogeneous and monodisperse formulations. The high negative zeta potential values observed indicate stability of the colloidal dispersion. X-ray diffraction and infrared spectroscopy analyzes showed the compatibility between the components of the samples. The encapsulation efficiency values obtained ranged from 40.0 to 66.1%, indicating satisfactory encapsulation of the oil in the matrix. The encapsulation process promoted the protection of the antioxidant activity of the oil, since a decrease in the percentage of inhibition was observed from 55.4% to values in the range of 41.4 to 51.3%. The addition of particles to the cassava starch matrix generated films with higher density, soluble and opaque than the control film. In general, the samples showed a homogeneous surface and mechanical properties slightly superior to those of the control film. The ability to inhibit the oxidant radical of the films (16.7 to 20.4%) indicates that the particles were able to promote greater protection of the active compounds of the oil during the film production and storage process, which provides its application for a variety of oxidation sensitive products

Comparison Of The Regiospecific Distribution From Triacylglycerols After Chemical And Enzymatic Interesterification Of High Oleic Sunflower Oil And Fully Hydrogenated High Oleic Sunflower Oil Blend By Carbon-13 Nuclear Magnetic Resonance

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)The nutritional and organoleptic attributes of oils can proceed via interesterification of oils blends catalyzed by enzymes or chemicals. Enzymatic interesterification processes are preferred due the regiospecific outcome. Traditionally, monitoring of distribution of fatty acids (FA) in glycerol backbone is performed by enzymatic and chromatographic methods that are time-consuming, involving a series of chemical manipulations employing large volumes of organic solvents. Alternatively, carbon-13 nuclear magnetic resonance (C-13 NMR) is a fast and reliable technique that could be applied to determine the saturated and unsaturated FA distribution of the triacylglycerols (TAGs) present in high oleic sunflower oil (SO) and fully hydrogenated high oleic sunflower oil (HSO) blends and their interesterification products. The enzymatic interesterification was conducted employing the immobilized lipase from Thermomyces lanuginosus (Lipozyme TL IM), the results show that the process was not completely regiospecific at sn-1,3 positions, due to the spontaneous acyl migration from position sn-2 to sn-1,3. (C) 2016 Elsevier Ltd. All rights reserved.212641647CAPESCNPqFAPESP [2012/09318-0]Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP

A Chemometric Model Applied to Fatty Acid Determination in Blood

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Changes in fatty acid profiles have been associated with several pathophysiological processes. Gas chromatography-mass spectrometry (GC-MS) data monitoring of selected ions was used with principal components analysis (PCA), revealing a set of relevant ions for quantification and characterization of fatty acids. This protocol was successfully applied to the analyses of fatty acids in different human blood lipids, allowing the quantification of several fatty acids and revealing their unsaturation numbers. Moreover the presences of contaminants, artifacts and co-eluitions in the chromatogram were also revealed without any additional analyses. Thus, fatty acid constituents of triglycerides, phospholipids and esters of cholesterol present in plasma and erythrocyte membranes were accurately determined, with repeatability, low limits of determination and of quantification.24101599+Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Agencia Nacional do Petroleo (Brazilian Agency for Petroleum - ANP, SP, Brazil)University of FloridaNSFFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP