Label free biosensor for screening estrogenic activity

640-645Estrogens and estrogen mimics prevalent in aquatic environment are of great environmental concern because of their endocrine disrupting and carcinogenic activities. Looking to the wide variety of natural as well as structurally different synthetic estrogen mimics, a reliable in-vitro assay is required for screening the estrogenic activity of endocrine disrupting compounds (EDCs). Surface plasmon resonance (SPR) is one of the most promising analytical tools to monitor the high-performance biomolecular interactions in a label free, real time format. Present paper demonstrates a facile SPR based affinity bioassay employing estrogen receptor-α, human (hERα) functionalized self assembled monolayer covalently bound onto the gold sensor chip as recognition species. A successful interaction of potential estrogen mimics with estrogen receptor is evidenced by net rise in SPR angle. The assay has been validated in terms of optimum experimental conditions and specificity with estrogen as a positive control showing maximum estrogenic activity. As a proof of concept, proposed affinity assay is tested for screening the estrogenic activity of progesterone, pregnenolone, tamoxifen, and bisphenol-A as representative examples of potential EDCs of different classes

Label free biosensor for screening estrogenic activity

Estrogens and estrogen mimics prevalent in aquatic environment are of great environmental concern because of their endocrine disrupting and carcinogenic activities. Looking to the wide variety of natural as well as structurally different synthetic estrogen mimics, a reliable in-vitro assay is required for screening the estrogenic activity of endocrine disrupting compounds (EDCs). Surface plasmon resonance (SPR) is one of the most promising analytical tools to monitor the high-performance biomolecular interactions in a label free, real time format. Present paper demonstrates a facile SPR based affinity bioassay employing estrogen receptor-a, human (hERa) functionalized self assembled monolayer covalently bound onto the gold sensor chip as recognition species. A successful interaction of potential estrogen mimics with estrogen receptor is evidenced by net rise in SPR angle. The assay has been validated in terms of optimum experimental conditions and specificity with estrogen as a positive control showing maximum estrogenic activity. As a proof of concept, proposed affinity assay is tested for screening the estrogenic activity of progesterone, pregnenolone, tamoxifen, and bisphenol-A as representative examples of potential EDCs of different classes