European stone fruit Yellows phytoplasma in Japanese plum and Myrobalan plum in Bosnia and Herzegovina

Stone fruits from commercial as well as abandoned orchards were evaluated for European Stone Fruit Yellows phytoplasma (ESFY) presence during 2004-2007 years. Orchards were monitored in western and southern districts of Bosnia and Herzegovina. In the first survey conducted in period of 2004 till 2005 the causal agent of ESFY was identified on peach (Prunus persica) and apricot (Prunus armeniaca) plants in both surveyed districts. During 2007, a new survey was performed and samples were taken from symptomatic and symptomless plants of European plum (Prunus domestica), Japanese plum (Prunus salicina), Myrobalan plum (Prunus cerasifera) and cherry (Prunus avium). Samples were analyzed using real-time PCR and nested PCR approaches. In this extended survey, the presence of ESFY phytoplasma was additionally identified in Japanese plum and myrobalan plum trees.Keywords: Bosnia and Herzegovina, myrobalan plum, Japanese plum, phytoplasma, ESFY, PC

Pome fruit viruses in Bosnia and Herzegovina

During autumn 2005 and summer 2006, field surveys were carried out to assess the sanitary status of pome fruit trees in Bosnia and Herzegovina. Inspections were done in the main pome fruit growing areas including 10 orchards, 2 nurseries and one varietal collection. A total of 65 apple and 50 pear cultivars were tested by biological indexing for the presence of Apple chlorotic leaf spot virus (ACLSV), Apple stem pitting virus (ASPV), Apple stem grooving virus (ASGV) and Apple mosaic virus (ApMV). The average infection level was 81%. Both species showed a similar infection rate (83% for apple and 78% for pear). The most frequent viruses of apple were ACLSV (72%) and ASPV (69%), and of pear ASGV (69%) and ACLSV (64%). The same samples were also tested by ELISA, with a lower virus detection rate compared to the biological indexing. Multiplex RT-PCR results of 20 randomly selected apple cultivars were in line with biological indexing. Results of our surveys report for the first time th e presence of ACLSV, ASPV, ASGV and ApMV on pome fruits in Bosnia and Herzegovina.Keywords: Malus, biological indexing, ELISA, multiplex RT-PCR, sanitary statu

Supplementary data for article: Paíga, P.; Lolić, A.; Hellebuyck, F.; Santos, L. H. M. L.; Correia, M.; Delerue-Matos, C. Development of a SPE-UHPLC-MS/MS Methodology for the Determination of Non-Steroidal Anti-Inflammatory and Analgesic Pharmaceuticals in Seawater. Journal of Pharmaceutical and Biomedical Analysis 2015, 106, 61–70. https://doi.org/10.1016/j.jpba.2014.06.017

Supplementary material for: [https://doi.org/10.1016/j.jpba.2014.06.017]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/1674

Detection by tissue printing hybridization of Pome fruit viroids in the mediterranean basin

Available data on the incidence and biodiversity of pome fruit viroids in the Mediterranean basin are limited. Before starting a research survey to fill this gap, a tissue-printing hydridization (TPH) method to detect Apple scar skin viroid (ASSVd), Pear blister canker viroid (PBCVd) and Apple dimple fruit viroid (ADFVd) has been developed and validated. Afterward, TPH was used in large-scale indexing of pome fruit viroids in Bosnia and Herzegovina, Malta, Lebanon and Turkey. A total of about 1,000 trees was randomly collected and tested. Positive results obtained by TPH were confirmed by at least one additional detection method (RT-PCR and/or Northern-blot hybridization) and viroids were finally identified by sequencing full-length cDNA clones. PBCVd was detected in 13%, 12.4% and 5.4% of the tested pear trees in Bosnia and Herzegovina, Malta and Turkey, respectively, showing a wider diffusion of this viroid than expected. In contrast, ASSVd was never detected and ADFVd was only found in symptomatic trees (cv. Starking Delicious) in Lebanon, confirming a restricted presence of these viroids in the Mediterranean basin. Altogether, these data support the use of TPH as an easy and valuable tool for exploring pome fruit viroid spread. Keywords: Viroid disease, viroid spread, pome fruit trees, detection methods, molecular hybridizatio

Antioxidant and Antimicrobial Activity of Some Tetradentate Schiff Bases and Their Cu(II) Complexes

Schiff bases, and their Cu(II) complexes, are known for their biological activity. In this work, antibacterial activity against Gram-negative strains of Escherichia coli, Pseudomonas aeruginosa and Staphylococcus pyogenes, as well as Gram-positive Staphylococcus pyogenes and Pseudomonas aeruginosa was studied, together with antifungal activity against Candida, Aspergillus, and Mucor strains. Also, technically simple, and rapid tests like ABTS, HORAC, and ORAC were used to investigate the antioxidant activity in order to compare obtained results with different type of tests

Supplementary data for article: Paíga, P.; Lolić, A.; Hellebuyck, F.; Santos, L. H. M. L.; Correia, M.; Delerue-Matos, C. Development of a SPE-UHPLC-MS/MS Methodology for the Determination of Non-Steroidal Anti-Inflammatory and Analgesic Pharmaceuticals in Seawater. Journal of Pharmaceutical and Biomedical Analysis 2015, 106, 61–70. https://doi.org/10.1016/j.jpba.2014.06.017

Supplementary material for: [https://doi.org/10.1016/j.jpba.2014.06.017]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/1674

Supplementary material for the article: Elshaflu, H.; Todorović, T. R.; Nikolić, M.; Lolić, A.; Višnjevac, A.; Hagenow, S.; Padrón, J. M.; García-Sosa, A. T.; Djordjevic, I. S.; Grubišic, S.; et al. Selenazolyl-Hydrazones as Novel Selective MAO Inhibitors with Antiproliferative and Antioxidant Activities: Experimental and In-Silico Studies. Frontiers in Chemistry 2018, 6 (JUL). https://doi.org/10.3389/fchem.2018.00247

Supplementary material for: [https://doi.org/10.3389/fchem.2018.00247]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2172

Supplementary data for article: Lolić, A.; Paíga, P.; Santos, L. H. M. L.; Ramos, S.; Correia, M.; Delerue-Matos, C. Assessment of Non-Steroidal Anti-Inflammatory and Analgesic Pharmaceuticals in Seawaters of North of Portugal: Occurrence and Environmental Risk. Science of the Total Environment 2015, 508, 240–250. https://doi.org/10.1016/j.scitotenv.2014.11.097

Supplementary material for: [https://doi.org/10.1016/j.scitotenv.2014.11.097]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/1653

Influence of the Structure on the Antioxidant Activity of Tetradentate Schiff Bases and their Copper(II) Complexes: Possible Mechanisms

The influence of the structure on the antioxidant activity of a series of Schiff bases and their copper(II) complexes as well as possible mechanisms of antioxidant activity were investigated. Schiff bases are derived from the condensation of ethane-1,2-diamine or propane-1,2-diamine and pentane-2,4-dione and/or 1-phenylbutane-1,3-dione. The corresponding complexes were synthesized. The antioxidant activity in vitro, determined using reversed-phase thin layer chromatography assay (RP TLC) based on the reaction with 2,2'-diphenyl-1-picrylhydrazyl, was expressed as Trolox equivalent antioxidant capacity (TEAC). The influence of the structure was observed on the basis of interpretable and informative descriptors calculated by hybrid density functional theory (DFT) methods. Structural features responsible for the antioxidant activity of the investigated compounds were discussed. Copper(II) complexes showed a significantly higher antioxidant activity than their corresponding ligands. Calculated quantum-chemical descriptors gave an insight to the reaction mechanism of the scavenging/antioxidant activity of tetradentate Schiff bases and their copper(II) complexes and two different reaction mechanisms were proposed

Cluster analysis of higher-education competitiveness in selected European countries

The subject of research in this paper is higher-education competitiveness on account of its impact on the enhancement of social and economic competitiveness, as well as on the growth of human capital and creation of social knowledge. The purpose of this paper is to group the selected European countries according to higher-education competitiveness, by means of the hierarchical cluster analysis method, with a special focus on the position of Serbia. Higher-education competitiveness in the chosen countries is analysed by means of three indicators of competitiveness: the ratio of the number of students per number of inhabitants, the number of students per number of employed, as well as the amount of budgetary funds allocated per student. The research results indicate different higher-education competitiveness in the analysed countries and also the fact that, according to this analysis, Serbia is in the group of countries with low competitiveness of higher education