We can only solve the problem with coordinated action:Two-factor authentification is a must

Digitisation is turning large companies into gold mines for cybercriminals. How can the public and private sectors arm themselves to combat this cyber threat

Als algoritmen routinewerk overnemen nekt dat ook expertise

Kunstmatige intelligentie gaat ons werk transformeren. Ook juristen ontkomen er niet aan. Als je tot voor kort het maken van een contract wilde automatiseren, moest je alle opties en keuzes vooraf bedenken en in het model stoppen. Je kreeg dus als resultaat wat je erin stopte, niets meer, niets minder. Nu voer je een zelflerend algoritme een grote stapel contracten en vindt het zelf zijn weg in alle relevante opties en bijbehorende bepalingen die ervaren advocaten door de jaren hebben bedacht. Het algoritme als neerslag van onze collectief opgebouwde contextuele ervaring in het opstellen van contracten, als dat maar geen banen gaat kosten

Closing the circle, to open a creative space: Can the intentions of avowedly psychoanalytic research methodologies be fulfilled in methods that are deeply congruent with this epistemology? A methodological study to inform future psychoanalytic research endeavours.

In this study, I have grappled with the challenge of developing a start-to-finish qualitative research method deeply congruent with the ontology and epistemology of psychoanalysis. I have drawn on the history of psychoanalytic research interviewing, most specifically the work of Hollway and Jefferson (Free Association Narrative Interview, 2000/2013) and Holmes (Reverie Research Method, 2017, 2019), which has been thoroughly analysed and taken forward by Archard (e.g. 2019, 2021). Through a process of sifting and synthesising existing literature, I have produced a nine-step guide for undertaking psychoanalytically informed interviews and four novel data-analysis questions. I operationalised my method with a research informant on the topic of her experience of Child and Adolescent Mental Health Service’s (CAMHS) Initial Assessments. Questions of ethics permeate discussions around taking psychoanalytic concepts outside the context in which they developed (e.g. Holmes, 2019). I offer a contribution to this debate through consideration of how the unconscious is theorised in interview-based research. I draw upon Bollas’s theory of the receptive unrepressed unconscious (e.g. 2006) and Bion’s theory of thinking (1962/1991) to counter criticisms of researchers veering beyond the research brief, into analysing the informants themselves. This can happen when researchers interpret contents of the informant’s repressed unconscious that may emerge in the research context. I illustrate how psychoanalytic supervision can be used in a novel way, contributing unconscious data from the freely associative conversation this entails. I then consider my data through the concept of parallel process (e.g. Morrissey & Tribe, 2001; Sumerel, 1994) as an ethical alternative to the controversial transport of the psychoanalytic concept of transference-countertransference dynamics out of the clinic setting. I chose substantial transcript excerpts for the vivid way in which they seem to capture the dynamic unconscious-to-unconscious communication process. I show how I explored these using my four data-analysis questions and in so doing, I take a step towards filling the methodological gap identified by Attride-Stirling (2001) regarding the need to analyse data in a systematic and clearly disclosed way. I am guided throughout by Bion’s (1970/2004) proposition of the ultimately unknowable nature of reality and Allison’s idea of a modest epistemology (cited in Stänicke et al., 2020)

8. klassi õpilaste tekstimõistmise tasemed bioloogiaõpikute kolme õppetunni materjali näitel

http://www.ester.ee/record=b4482700*es

Closing the circle, to open a creative space: Can the intentions of avowedly psychoanalytic research methodologies be fulfilled in methods that are deeply congruent with this epistemology? A methodological study to inform future psychoanalytic research endeavours.

In this study, I have grappled with the challenge of developing a start-to-finish qualitative research method deeply congruent with the ontology and epistemology of psychoanalysis. I have drawn on the history of psychoanalytic research interviewing, most specifically the work of Hollway and Jefferson (Free Association Narrative Interview, 2000/2013) and Holmes (Reverie Research Method, 2017, 2019), which has been thoroughly analysed and taken forward by Archard (e.g. 2019, 2021). Through a process of sifting and synthesising existing literature, I have produced a nine-step guide for undertaking psychoanalytically informed interviews and four novel data-analysis questions. I operationalised my method with a research informant on the topic of her experience of Child and Adolescent Mental Health Service’s (CAMHS) Initial Assessments. Questions of ethics permeate discussions around taking psychoanalytic concepts outside the context in which they developed (e.g. Holmes, 2019). I offer a contribution to this debate through consideration of how the unconscious is theorised in interview-based research. I draw upon Bollas’s theory of the receptive unrepressed unconscious (e.g. 2006) and Bion’s theory of thinking (1962/1991) to counter criticisms of researchers veering beyond the research brief, into analysing the informants themselves. This can happen when researchers interpret contents of the informant’s repressed unconscious that may emerge in the research context. I illustrate how psychoanalytic supervision can be used in a novel way, contributing unconscious data from the freely associative conversation this entails. I then consider my data through the concept of parallel process (e.g. Morrissey & Tribe, 2001; Sumerel, 1994) as an ethical alternative to the controversial transport of the psychoanalytic concept of transference-countertransference dynamics out of the clinic setting. I chose substantial transcript excerpts for the vivid way in which they seem to capture the dynamic unconscious-to-unconscious communication process. I show how I explored these using my four data-analysis questions and in so doing, I take a step towards filling the methodological gap identified by Attride-Stirling (2001) regarding the need to analyse data in a systematic and clearly disclosed way. I am guided throughout by Bion’s (1970/2004) proposition of the ultimately unknowable nature of reality and Allison’s idea of a modest epistemology (cited in Stänicke et al., 2020)

Importance of disulphide bonds for vaccinia virus L1R protein function

L1R, a myristylated late gene product of vaccinia virus, is essential for formation of infectious intracellular mature virions (IMV). In its absence, only viral particles arrested at an immature stage are detected and no infectious progeny virus is produced. Previous studies have shown that the L1R protein is exclusively associated with the IMV membrane and that myristylation is required for correct targeting. The L1R protein contains six cysteine amino acid residues that have all been shown to participate in intramolecular disulphide bonds. However, it was not clear what role, if any, the disulfide bonds play in the membrane topology of the L1R protein. To address this question, a comprehensive library of L1R mutants in which the cysteine residues have been mutated to serine (either individually or in combination) were tested for their ability to rescue a L1R conditional lethal mutant virus under non-permissive conditions. Much to our surprise, we determined that C57 was not essential for production of infectious IMV. These results suggest that protein disulphide isomerases may be involved in reorganization of disulfide bonds within the L1R protein