Stressprävention im Jugendalter. Entwicklung eines Trainingsprogramms

Beyer A, Lohaus A. Stressprävention im Jugendalter. Entwicklung eines Trainingsprogramms. In: Röhrle B, ed. Prävention und Gesundheitsförderung, Band III: Kinder und Jugendliche. Fortschritte der Gemeindepsychologie und Gesundheitsförderung. Vol 16. Tübingen: DGVT-Verlag; 2007

Stressprävention im Jugendalter. Entwicklung eines Trainingsprogramms

Beyer A, Lohaus A. Stressprävention im Jugendalter. Entwicklung eines Trainingsprogramms. In: Röhrle B, ed. Prävention und Gesundheitsförderung, Band III: Kinder und Jugendliche. Fortschritte der Gemeindepsychologie und Gesundheitsförderung. Vol 16. Tübingen: DGVT-Verlag; 2007

Problemlösen und Entspannung als Stressbewältigungsstrategien im Kindesalter

Lohaus A, Klein-Heßling J. Problemlösen und Entspannung als Stressbewältigungsstrategien im Kindesalter. In: Röhrle B, Sommer G, eds. Prävention und Gesundheitsförderung. Fortschritte der Gemeindepsychologie und Gesundheitsförderung. Vol 4. Tübingen: DGVT-Verlag; 1999: 233-251

Trypsin-Free Cultivation of 3D Mini-Tissues in an Adaptive Membrane Bioreactor

The production of large scaffold-free tissues is a key challenge in regenerative medicine. Nowadays, temperature-responsive polymers allow intact tissue harvesting without needing proteolytic enzymes. This method is limited to tissue culture plastic with limited upscaling capacity and plain process control. Here, a thermoresponsive hollow fiber membrane bioreactor is presented to produce large scaffold-free tissues. Intact tissues, rich in cell-to-cell connections and ECM, are harvested from a poly(N-vinylcaprolactam) microgel functionalized poly(ether sulfone)/poly(vinylpyrrolidone) hollow fiber membrane by a temperature shift. The harvested 3D tissues adhere in successive cultivation and exhibit high vitality for several days. The facile adsorptive coating waives the need for extensive surface treatment. The research is anticipated to be a starting point for upscaling the production of interconnected tissues enabling new opportunities in regenerative medicine, large-scale drug screening on physiological relevant tissues, and potentially opening new chances in cell-based therapies. © 2020 The Authors. Advanced Biosystems published by Wiley-VCH Gmb

Trypsin‐Free Cultivation of 3D Mini‐Tissues in an Adaptive Membrane Bioreactor

The production of large scaffold-free tissues is a key challenge in regenerative medicine. Nowadays, temperature-responsive polymers allow intact tissue harvesting without needing proteolytic enzymes. This method is limited to tissue culture plastic with limited upscaling capacity and plain process control. Here, a thermoresponsive hollow fiber membrane bioreactor is presented to produce large scaffold-free tissues. Intact tissues, rich in cell-to-cell connections and ECM, are harvested from a poly(N-vinylcaprolactam) microgel functionalized poly(ether sulfone)/poly(vinylpyrrolidone) hollow fiber membrane by a temperature shift. The harvested 3D tissues adhere in successive cultivation and exhibit high vitality for several days. The facile adsorptive coating waives the need for extensive surface treatment. The research is anticipated to be a starting point for upscaling the production of interconnected tissues enabling new opportunities in regenerative medicine, large-scale drug screening on physiological relevant tissues, and potentially opening new chances in cell-based therapies. © 2020 The Authors. Advanced Biosystems published by Wiley-VCH Gmb