Effects of in-hospital diuretic therapy on electrolytes concentration, renal function and survival in 85 dogs with acute congestive heart failure

Critically hill patients with acute congestive heart failure (CHF) may often show haemoconcentration, dysnatremia, dyskalemia and increased azotemia, due to aggressive diuretic therapy. Haemocon-centration is associated with lower risk of mortality, while dysnatremia and dyskalemia are associated with higher mortality in human medicine. The aim of this study was to retrospectively evaluate the impact of in-hospital diuretic therapy for CHF on selected laboratory parameters and long-term mortality. Dogs with clinical and radiological evidence of CHF confirmed by echocardiography were included. Blood samples collected through out the hospitalization at presentation (T0) and discharge (T1) were: venous blood gas analysis (VBGA), serum creatinine (sCr), blood ureanitrogen (BUN), microhematocrit (Htc) and total proteins (TP). Length of hospital stay, ACVIM class and other clinical indices were recorded. Haemoconcentration was defined as a simultaneous increase in Htc and total protein. A total of 85 dogs (45 male and 40 female; mean age 11.07 \ue003 2.54 years; mean weight 8.86 \ue003 6.92 kg) were included. Thirty-six dogshad previous episodes of CHF. Mean length of in-hospital stay was 31.15 \ue003 17.35 hours. Treatment protocol included a single furosemide endovenous bolus at 2 mg/kg followed by multiple 1 mg/kg bolus/hour until respiratory rate reach 40 respiratory rate. Each dog received 8.6 \ue003 2.8 mg/kg and 11.1 \ue003 2.9 mg/kg furosemide in 24 and 48 hours respectively. Ten dogs received higher furosemide doses or torasemide bolus. Haemoconcentration was reached in the 33% of dogs. Considering the VBGA and biochemistry results, the number of dogs showing extra-range values (T0-T1) were respectively: hyponatremia (10-23), hypernatremia (13-17), hypokalemia (18-30), hyperkalemia (10-10), hypocloremia (46-61), increased BUN (26-34), increased sCr (3-8). Fourty-one dogs experienced cardiac death, 12 during hospitalization, the remaining dogs between 3 and 721 days after admission. Stepwise backward regression demonstrated haemoconcentration (HR 0.33) and disnatremia (HR 2.85) influence over outcome. Statisti-cally significant correlation (Pearson) was seen between furosemide dose and kalemia (r = 120.32, P = 0.014) and between BUN and sCr (r = 0.27, P = 0.021). No correlation was seen between furosemide dose and the variables sCr, BUN, Htc and between sCr and Htc. In conclusion, haemoconcentration and disnatremia affected the out-come in dogs with CHF. Haemoconcentration was associated withlower risk of mortality and had to be considered a target in CHF therapy. In-hospital diuretic therapy increased electrolyte disorder due to loop diuretics inhibition of the renal Na, K, Cl cotransporter in the Henle's loop and disnatremia was a risk factor for adverse outcome. Diuretics doses and haemoconcentration didn't play a direct role in inducing renal disfunction

De novo a-to-i rna editing discovery in lncrna

Background: Adenosine to inosine (A-to-I) RNA editing is the most frequent editing event in humans. It converts adenosine to inosine in double-stranded RNA regions (in coding and noncoding RNAs) through the action of the adenosine deaminase acting on RNA (ADAR) enzymes. Long non-coding RNAs, particularly abundant in the brain, account for a large fraction of the human transcriptome, and their important regulatory role is becoming progressively evident in both normal and transformed cells. Results: Herein, we present a bioinformatic analysis to generate a comprehensive inosinome picture in long non-coding RNAs (lncRNAs), using an ad hoc index and searching for de novo editing events in the normal brain cortex as well as in glioblastoma, a highly aggressive human brain cancer. We discovered >10,000 new sites and 335 novel lncRNAs that undergo editing, never reported before. We found a generalized downregulation of editing at multiple lncRNA sites in glioblastoma samples when compared to the normal brain cortex. Conclusion: Overall, our study discloses a novel layer of complexity that controls lncRNAs in the brain and brain cancer

Dynamics of two coupled vortices in a spin valve nanopillar excited by spin transfer torque

We investigate the dynamics of two coupled vortices driven by spin transfer. We are able to independently control with current and perpendicular field, and to detect, the respective chiralities and polarities of the two vortices. For current densities above $J=5.7*10^7 A/cm^2$, a highly coherent signal (linewidth down to 46 kHz) can be observed, with a strong dependence on the relative polarities of the vortices. It demonstrates the interest of using coupled dynamics in order to increase the coherence of the microwave signal. Emissions exhibit a linear frequency evolution with perpendicular field, with coherence conserved even at zero magnetic field

Multiple retrospective analysis of survival and evaluation of 4 cardiac death predictors in a population of dogs affected by 5 degenerative mitral valve disease in ACVIM class C treated 6 with different therapeutic protocols

Clinical records of dogs with spontaneous degenerative mitral valve disease (DMVD) with clinical signs related to congestive heart failure (CHF) that had been recruited during routine clinical practice, between 2001 and 2018 at the Cardiology Unit of the Veterinary Teaching Hospital (University of Milan) were included in this retrospective cohort study. Baseline echocardiographic data were evaluated. Median survival times (MSTs) were calculated. Data on therapeutic treatment, ISACHC or ACVIM classes were reviewed based on the inclusion period and type of endpoint (i.e. cardiac death or death for other causes). The main goal of this data review was to retrospectively evaluate 259 clinical records of subjects belonging to ACVIM C class examined between 2001 to 2018 together with the 202 examined between 2010 to 2018. The MSTs of these subjects was 531 d (2001-2018) and 335.5 d (2010-2018), respectively. Univariate survival regression analysis for subjects included from 2010 to 2018 showed the following variables as being significantly related to cardiac death (CD): LA/Ao ratio (HR 2.754, p=0.000), E wave (HR 2.961, p=0.000), E/A ratio (HR 1.372, p=0.000), EDVI (HR 1.007, p=0.000), ESVI (HR 1.012, p=0.026), Allo(d) (HR 4.018, p=0.000) andAllo(s) (HR 2.674, p=0.049), age (HR 1.006, p=0.009) and PH severity (HR=1.309, p=0.012). Multivariate analysis, adjusted for age, showed that the only variable that determined a statistically significant difference in MST was PH severity (HR 1.334, p=0.033). The type of therapeutic treatment within this class was not significant for the MST of the subjects

Synthesis and biological evaluation of new bis-indolinone derivatives endowed with cytotoxic activity

A series of new Knoevenagel adducts, bearing two indolinone systems, has been synthe-sized and evaluated on 60 human cancer cell lines according to protocols available at the National Cancer Institute (Bethesda, MD, USA). Some derivatives proved to be potent antiproliferative agents, showing GI50 values in the submicromolar range. Compound 5b emerged as the most active and was further studied in Jurkat cells in order to determine the effects on cell-cycle phases and the kind of cell death induced. Finally, oxidative stress and DNA damage induced by compound 5b were also analyzed

Graphene-assisted control of coupling between optical waveguides

The unique properties of optical waveguides electrically controlled by means of graphene layers are investigated. We demonstrate that, thanks to tunable losses induced by graphene layers, a careful design of silicon on silica ridge waveguides can be used to explore passive PT-symmetry breaking in directional couplers. We prove that the exceptional point of the system can be probed by varying the applied voltage and we thus propose very compact photonic structures which can be exploited to control coupling between waveguides and to tailor discrete diffraction in arrays

Design, fabrication, and characterization of deep-etched waveguide gratings

One-dimensional (1-D) deep-etched gratings on a specially grown AlGaAs wafer were designed and fabricated. The gratings were fabricated using state-of-the-art electron beam lithography and high-aspect-ratio reactive ion etching (RIE) in order to achieve the required narrow deep air slots with good accuracy and reproducibility. Since remarkable etch depths (up to 1.5 /spl mu/m), which completely cut through the waveguide core layer, have been attained, gratings composed of only five periods (and, thus, shorter than 6 /spl mu/m) have a bandgap larger than 100 nm. A defect was introduced by increasing the width of the central semiconductor tooth to create microcavities that exhibit a narrow transmission peak (less than 7 nm) around the wavelength of 1530 nm. The transmission spectra between 1460 and 1580 nm have been systematically measured, and the losses have been estimated for a set of gratings, both with and without a defect, for different periods and air slot dimensions. Numerical results obtained via a bidirectional beam propagation code allowed the evaluation of transmissivity, reflectivity, and diffraction losses. By comparing experimental results with the authors' numerical findings, a clear picture of the role of the grating's geometric parameters in determining its spectral features and diffractive losses is illustrated

Mitophagy contributes to endothelial adaptation to simulated microgravity

Exposure to real or simulated microgravity is sensed as a stress by mammalian cells, which activate a complex adaptive response. In human primary endothelial cells, we have recently shown the sequential intervention of various stress proteins which are crucial to prevent apoptosis and maintain cell function. We here demonstrate that mitophagy contributes to endothelial adaptation to gravitational unloading. After 4 and 10 d of exposure to simulated microgravity in the rotating wall vessel, the amount of BCL2 interacting protein 3, a marker of mitophagy, is increased and, in parallel, mitochondrial content, oxygen consumption, and maximal respiratory capacity are reduced, suggesting the acquisition of a thrifty phenotype to meet the novel metabolic challenges generated by gravitational unloading. Moreover, we suggest that microgravity induced-disorganization of the actin cytoskeleton triggers mitophagy, thus creating a connection between cytoskeletal dynamics and mitochondrial content upon gravitational unloading

Sistemas agroflorestais agroecológicos em Rondônia: classes de solos e crescimento de espécies florestais.

Sistemas agroflorestais são associações de cultivos anuais com espécies florestais e em alguns casos pastagens e/ou animais, escalonados no tempo e no espaço. Este trabalho teve por objetivo caracterizar alguns sistemas agroflorestais agroecológicos em Rondônia do ponto de vista da classe de solo e crescimento de espécies florestais. Foram visitados 24 sistemas em seis municípios, as áreas georeferenciadas, elaborado mapa de classe de solos e avaliados crescimento em altura e DAP de espécies florestais. Foi verificado que apesar do nível de fertilidade do solo ser considerado suficiente em alguns locais, o crescimento das espécies foi prejudicado pelo espaçamento inadequado. Os sistemas estudados estão situados em sua maioria em Latossolo Vermelho Eutrófico, e apenas um em Cambissolo Háplico Distrófico

Nutrientes e biomassa em sistemas agroflorestais com ênfase no cupuaçuzeiro, em solo de baixa fertilidade.

O uso de sistemas agroflorestais é uma maneira de minimizar a degradação do solo causada pela agricultura de derruba e queima. Este trabalho teve por objetivo modelos de sistemas agroflorestais apropriados às condições de solo de baixa fertilidade. A área experimental é localizada no estado Rondônia. noroeste da Amazônia brasileira, latossolo amarelo, textura argilosa. O experimento foi composto pelos tratamentos: 1) castanheira-do-Brasil x Cupuaçuzeiro; 2) freijó-louro x cupuaçuzeiro; 3) pupunheira x cupuaçuzeiro; 4) castanheira-do-Brasil; 5) Freijó-louco; 6) Pupunheira. Foram avaliadas as produções de frutos, o crescimento das essencias florestais, a imobilização e a exportação de nutrientes e a capacidade de ciclagem de nutrientes nos sistemas. A partir dos resultados deste estudo, a castanheira-do-brasil se mostrou boa opção para associar com cupuaçuzeiro em solos de baixa fertilidade; na formação de sistemas agroflorestais, tendo o cupuaçuzeiro como componente principal, onde o freijó-louro e a pupunheira estejam presentes, deve-se considerar a utilização de adubação química; a serrapilheira no sistema castanheira x cupuaçuzeiro representou uma fonte de ingresso de nutrientes para a produção de frutos de cupuaçu; a produção de biomassa aérea da castanheira-do-brasil não foi afetada pela consorciação, enquanto a do freijó-louro sim.bitstream/item/70944/1/BP1-sistemasagroflorestais.pd